1.Effects of salicylates on the susceptibility of klebseilla pneumoniae to cephalosporins.
Phil Youl RYU ; Seung Ryong GOO ; Sun Sick CHUNG
Journal of the Korean Society for Microbiology 1993;28(2):95-103
No abstract available.
Cephalosporins*
;
Pneumonia*
;
Salicylates*
2.Expression of antigens during HL-60 cell differentiation.
Jong Suk OH ; In Chol KANG ; Phil Youl RYU ; Boo Ahn SHIN ; Hyun Chul LEE
Journal of the Korean Cancer Association 1991;23(3):536-543
No abstract available.
HL-60 Cells*
;
Humans
3.In vitro effect of ciprofloxacin on vibrio vulnificus.
Phil Youl RYU ; Boo Ahn SHIN ; Joon Haeng RHEE ; Sun Sik CHUNG ; Tai Hew AHN
Journal of the Korean Society for Microbiology 1992;27(4):345-357
No abstract available.
Ciprofloxacin*
;
Vibrio vulnificus*
;
Vibrio*
4.In vitro ractericidal activity of quinolones against vibrio vulnificus.
Phil Youl RYU ; Jung Inn CHOI ; Joon Haeng RHEE ; Sun Sik CHUNG
Journal of the Korean Society for Microbiology 1992;27(5):449-456
No abstract available.
Quinolones*
;
Vibrio vulnificus*
;
Vibrio*
5.Study on the Effectivity and Stability of the Fortified Ophthalmic Solutions.
Jai Bong KIM ; Sang Ki JEONG ; Yeoung Geol PARK ; Phil Youl RYU
Journal of the Korean Ophthalmological Society 1993;34(5):468-473
The questions concerning storage temperature and storage duration of the fortified ophthalmic antibiotic solutions are raised. The aim of the current study is to evaluate the influence of fortified ophthalmic antibiotic solutions on its biological and physical properties according to the storage temperature and duration as time goes by. We examined the changes of pH, absorbance spectra, and anti-microbial activity of cefradine(63mg/ml)and gentamicin sulfate(13.6mg/ml) over a four-week period. The results were as follows: There was no difference between the potency of fortified GM solutions stored at 4 degrees C and that at 24 degrees C for 4 weeks. The cefradine stored 24 degrees C exhibited significant changes in both its tJotency and stability after ithe seventh day of storage. The cefradine stored at 4 degrees C exhibited changes in Its absorbance spectra day by day and potency after 14th day.
Cephradine
;
Gentamicins
;
Hydrogen-Ion Concentration
;
Ophthalmic Solutions*
6.The Role of Vibrio vulnificus Capsular Polysaccharide as a Virulence Factor.
Sun Sik CHUNG ; Hyun Chul LEE ; Boo Ahn SHIN ; Shee Eun LEE ; Phil Youl RYU ; Won Seok KIM
Journal of the Korean Society for Microbiology 1998;33(5):425-434
To evaluate the role of capsular polysaccharide (CPS) as a virulence factor, the interaction of V. vulnificus with mouse peritoneal macrophages and serum, which are involved in the clearance of bacteria from blood and other tissues, were examined. In this study, MO6-24/0 (wild strain; hemolysin- and capsule-positive), MO6-24/I' (acapsular spontaneous mutant), CVD 752 (acapsular transposon mutant), and CVD 707 (hemolysin-negative and capsule-positive mutant) were used. The strain with CPS (MO6-24/0 and CVD 707) were more resistant to phagocytosis by mouse peritoneal macrophages compared with acapsular strains (MO6-24/T and CVD 752), and the resistance to phagocytosis was not changed by serum opsonin in the capsular strains. Acapsular strains were more susceptible to serum bactericidal activity than the capsular strains through the classical complement pathway. MO6-24/0 strain were detected in blood, spleen, liver and lung at 4 hours after intraperitoneally infection, whereas CVD 752 were not detected. All tested strains could induced the transcription of inflammatory cytokine gene such as IL-1, IL-6, IL-10 and TNF-u, and their inductions were not decreased by cytochalasin B treatment. This results demonstrate that CPS of V. vulnificus plays an important role in V. vulnificus infection through interfering nonspecific host defense system such as blood clearance and phagocytosis.
Animals
;
Bacteria
;
Complement Pathway, Classical
;
Cytochalasin B
;
Interleukin-1
;
Interleukin-10
;
Interleukin-6
;
Liver
;
Lung
;
Macrophages, Peritoneal
;
Mice
;
Phagocytosis
;
Spleen
;
Vibrio vulnificus*
;
Vibrio*
;
Virulence*
7.Effects of cAMP and cGMP on the blockade of TCR-CD3-mediated cytoplasmic free calcium increased by cholera toxin in human peripheral blood T lymphocytes.
Boo Ahn SHIN ; Phil Youl RYU ; Shee Eun LEE ; Mee Young JANG ; Hyuck IM ; Hyun Chul LEE
Korean Journal of Immunology 1992;14(2):287-295
No abstract available.
Calcium*
;
Cholera Toxin*
;
Cholera*
;
Cytoplasm*
;
Humans*
;
T-Lymphocytes*
8.Effects of Biofilm Formation on The Antimicrobial Susceptibility of Staphylococcus aureus.
Kyung Jin CHO ; Shen JIN ; Jinghua CUI ; Taek Rim YOON ; Phil Youl RYU
Journal of Bacteriology and Virology 2008;38(4):197-205
Staphylococcus aureus induces chronic infection in form of biofilm that exists in the host cells and arthroplastic prosthesis surface. In this study, the biofilm formation ability of S. aureus clinically isolated from bacteremia patients, biofilm processing and relationship of resistance to antibiotics, and difference of biofilm formation ability on different prosthetic material surfaces were studied. All of them formed biofilm and especially 6 strains of S. aureus had high ability of biofilm formation. In addition, it was found that some strains with higher biofilm formation ability make more higher polysaccharide layer production. When S. aureus ATCC 25923 forms biofilm, minimal bactericidal concentration (MBC) of biofilm bacteria is more increased than that of the planktonic state bacteria about one thousand folds. Especially, after 6 hours from starting on biofilm formation, the resistance to antibiotics was increased by more than 256 microgram/ml of MBC to every antibiotics and after 8 hours prominent increase (more than 4096 microgram/ml) was noted. Biofilm formation after bacterial adherence to plastic cover-slip was increased with time-dependent manner. Microcolonies were formed after 5 hours from a point that bacteria adhere to plastic cover-slip surface and after 6 hours biofilm was diffusely formed on entire surface, and then after 8 hours very thick biofilm was formed. Thicker biofilm was found on cobalt-chromium than titanium surface. These results suggest that titanium alloy materials are better than cobalt-chromium to minimize S. aureus biofilm formation on the arthroplastic material surface. Also, when microcolonies are formed after adherence of S. aureus to the arthroplastic material surface, resistance to antibiotics is starting.
Alloys
;
Anti-Bacterial Agents
;
Bacteremia
;
Bacteria
;
Biofilms
;
Humans
;
Plankton
;
Plastics
;
Prostheses and Implants
;
Staphylococcus
;
Staphylococcus aureus
;
Titanium
9.Genotypic and Phenotypic Characterization of Enteropathogenic Escherichia coli Isolated from Diarrheal Patients in Gwangju.
Sun Hee KIM ; Hyeon Je SONG ; Jae Keun CHUNG ; Dong Rong HA ; Phil Youl RYU ; Jong Bin LEE
Journal of Bacteriology and Virology 2006;36(3):167-174
Enteropathogenic Escherichia coli (EPEC) have been implicated in human diarrhea in several countries. Central to EPEC-mediated disease is its ability to cause intestinal lesions, known as attaching and effacing (A/E) lesion. We investigated 92 EPEC strains isolated from patients with diarrhea in Gwangju for their genotypic and phenotypic characteristics. Sixteen (17.4%) of all strains were found to be typical EPEC because they were bfpA gene positive by PCR. The most of typical EPEC isolates (87.5%) showed a localized adhesion (LA) pattern in Hep-2 cell adherence assay, whereas, only 11 atypical EPEC isolates (14.5%) were adhered to Hep-2 cells in a localized manner. Thirteen of the EPEC strains studied belonged to classical O-serogroups of EPEC and 7 isolates were classified as nonclassical EPEC serogroup and the other isolates could not be serotyped with our antisera. The subtypes of eae, tir, espA and espB genes which are major virulence genes concerned of A/E lesion on chromosome were analyzed by multiplex PCR for finding the original resource. The results showed that the composition of these genes subtypes was homogenous and heterogenous in 12 and 26 isolates, respectively. The others were non-determined type in terms of the gene subtype because of genetic diversity of intimin-coding eae genes. Our findings indicated that EPEC isolates from patients with diarrhea were diverse genetically and phenotypically, which require further study in regard to their virulence and epidemiological significance.
Diarrhea
;
Enteropathogenic Escherichia coli*
;
Genetic Variation
;
Gwangju*
;
Humans
;
Immune Sera
;
Multiplex Polymerase Chain Reaction
;
Polymerase Chain Reaction
;
Virulence
10.A Case of Endobronchial Fibroepithelial Polyp.
Young KIM ; Chang Youl LEE ; Sung Jun HWANG ; Je Phil CHOI ; Hyuing Jung KIM ; Chul Min AHN ; Young Hoon RYU ; Sang Jin KIM
Tuberculosis and Respiratory Diseases 2001;51(6):609-614
Benign endobronchial tumors are rare diseases with an incidenced of between 1 and 5% of all lung tumors. An endobronchial fibroepithelial polyp is an extremely rare form of benign bronchial tumor. Clinically, an endobronchial fibroepithelial polyp causes an airway obstruction and obstructive pneumonitis as does other endobronchial tumors. Therefore, it is important to differentiate an endobronchial fibroepithelial polyp pathologically from other benign endobronchial tumors and bronchogenic carcinomas. Here, we report a case of an endobronchial fibroepithelial polyp, in a 25-year-old man who had suffered from chest discomfort upon deep breathing with a brief review of the relevant literature.
Adult
;
Airway Obstruction
;
Carcinoma, Bronchogenic
;
Humans
;
Lung
;
Pneumonia
;
Polyps*
;
Rare Diseases
;
Respiration
;
Thorax