1.An immunosynapse - sites of the membrane glucosyl- phosphate idylinositol
Journal of Medical Research 2001;15(2):42-44
The extracellular proteins bound with the membrane through the sites of glucosyl-phosphat idylinositol (GPI) to generate the complexes of protein with GPI. The GPIs have concentrated structure to generate glycolipid rich sites in the membrane. These sites have inert property with detergent. The GPI bound extracellular proteins can transmit the sign to cells. This sign is the same as this transmitted from receptor MD. The exist of structure GPI may be an example of the concentration of many suitable molecules which intact mutually to implement optimal a function. These GPI related with the costimulation phenomena and their play likes as a synapse MD
Membranes
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Proteins
2.The Association of Cytokines with Severe Dengue in Children
Julia N.A. Mangione ; Nguyen Tien Huy ; Nguyen Thi Phuong Lan ; Evaristus Chibunna Mbanefo ; Tran Thi Ngoc Ha ; Lam Quoc Bao ; Cao Thi Phi Nga ; Vo Van Tuong ; Tran Van Dat ; Tran Thi Thuy ; Ha Manh Tuan ; Vu Thi Que Huong ; Kenji Hirayama
Tropical Medicine and Health 2014;42(4):137-144
Background: Dengue virus infection is a major public health problem. A hypothesis put forward for severe dengue is the cytokine storm, a sudden increase in cytokines that induces vascular permeability. Previous studies and our recent meta-analysis showed that IL-6, IL-8, IFNγ, TNFα, VEGF-A and VCAM-1 are associated with dengue shock syndrome. Therefore, in this study we aim to validate the association of these cytokines with severe dengue. Methods & Findings: In a hospital based-case control study in Vietnam, children with dengue fever, other febrile illness and healthy controls were recruited. Dengue virus infection was confirmed by several diagnostic tests. Multiplex immunoassay using Luminex technology was used to measure cytokines simultaneously. A positive association with dengue shock syndrome was found for VCAM-1, whereas a negative association was found for IFNγ. Furthermore, multivariate logistic analysis also showed that VCAM-1 and IFNγ were independently correlated with dengue shock syndrome. Conclusion: IFNγ and VCAM-1 were associated with dengue shock syndrome, although their role in the severe dengue pathogenesis remains unclear. Additional studies are required to shed further light on the function of these cytokines in severe dengue.
3.The Association of Cytokines with Severe Dengue in Children
Julia N.A. Mangione ; Nguyen Tien Huy ; Nguyen Thi Phuong Lan ; Evaristus Chibunna Mbanefo ; Tran Thi Ngoc Ha ; Lam Quoc Bao ; Cao Thi Phi Nga ; Vo Van Tuong ; Tran Van Dat ; Tran Thi Thuy ; Ha Manh Tuan ; Vu Thi Que Huong ; Kenji Hirayama
Tropical Medicine and Health 2014;():-
Background: Dengue virus infection is a major public health problem. A hypothesis put forward for severe dengue is the cytokine storm, a sudden increase in cytokines that induces vascular permeability. Previous studies and our recent meta-analysis showed that IL-6, IL-8, IFNγ, TNFα, VEGF-A and VCAM-1 are associated with dengue shock syndrome. Therefore, in this study we aim to validate the association of these cytokines with severe dengue. Methods & Findings: In a hospital based case control study in Vietnam, children with dengue fever, other febrile illness and healthy controls were recruited. Dengue virus infection was confirmed by several diagnostic tests. Multiplex Immunoassay using Luminex technology was used to measure cytokines simultaneously. A positive association with dengue shock syndrome was found for VCAM-1, whereas a negative association was found for IFNγ. Furthermore, the multivariate logistic analysis also showed that VCAM-1 and IFNγ were independently correlated with dengue shock syndrome. Conclusion: IFNγ and VCAM-1 were associated with dengue shock syndrome, although their role in the severe dengue pathogenesis remains unclear. Additional studies are required to further investigate the function of these cytokines in severe dengue.
4. Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack
Dao Thi Thanh HIEN ; Tran Phi LONG ; Tran Phuong THAO ; Jeong-Hyung LEE ; Duong Thu TRANG ; Nguyen Thi Thu MINH ; Pham VAN CUONG ; Nguyen Hai DANG ; Do Thi Ngoc LAN ; Nguyen Tien DAT
Asian Pacific Journal of Tropical Biomedicine 2019;9(1):18-23
Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The level of NO produced in the culture media was determined by Griess method. The iNOS and COX-2 protein expressions were analyzed by Western blot. The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model. Mice mortality was monitored for 5 days after injection of LPS. The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques. Results: The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vivo models. The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions. In the septic shock model, ELA dose-dependently protected mice from LPS-induced mortality. Further study on the isolated components of ELA indicated that 9,10-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract. Conclusions: These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS, and COX-2 and protects mice from LPS-induced mortality in septic shock model.