The investigation on the herbal of Euphorbia humifusa Wild. was carried out to find its anti-HBV constituents. The isolation and purification were performed by chromatography such as macroporous resin, polyamide, Sephadex LH-20, MCI GEL CHP 20P and so on. Based on the spectral analysis, seven phenols were identified as brevifolin (1), brevifolin carboxylic acid (2), methyl brevifolincarboxylate (3), phyllanthussin E methyl ester (4), sanguisorbic acid dilactone (5), 3,3'-2-di-O-methyl ellagic acid (6), ellagic acid (7). Among them, Compounds 2-6 were isolated from this plant for the first time.
Euphorbia ; chemistry ; Phenols ; chemistry ; isolation & purification

Eight phenolic compounds (1-7) were isolated from the methanol extract of the root of Rhodiola dumulosa. Their structures were identified as kaemperol (1), Quercetin (2), Gallic acid (3), (+) -Isolariciresinol-3alpha-O-beta-D-glucopyranoside (4), (-)-Isolariciresinol-3alpha-O-beta-D-glucopyra-noside (5), kaemperol-3-O-beta-D-glucopyranoside-7-alpha-O-L-rhamnoside (6), rutin (7) respectively on the basis of chemical and spectroscopic evidence. The compounds 3-7 were isolated from R. dumulosa for the first time.
Kaempferols ; chemistry ; isolation & purification ; Phenols ; chemistry ; isolation & purification ; Quercetin ; chemistry ; isolation & purification ; Rhodiola ; chemistry ; Rutin ; chemistry ; isolation & purification

OBJECTIVETo study the phenolic constituents of Dendrobium aphyllum.

METHODVarious chromatographic techniques were used to isolate and purify the constituents, their physico-chemical properties and spectral data were employed to elucidate their structures.

RESULTNine bibenzyls and two benzylethanyl compounds were isolated and identified as: moscatilin (1), gigantol (2), batatasin (3), tristin (4), 3, 5, 4'-trihydroxylbibenzyl (5), 3, 5-dimethoxyl-4, 4'-dihydroxylbibenzyl (6), moscatin (7), 2, 4, 7-trihydroxyl-9, 10-dihydrophenanthrene (8), hircinol (9), 2-(4-hydroxyphenyl) ethyl-beta-D-glucopyranoside, salidroside (10) and p-hydroxylbenzylacetic acid (11).

CONCLUSIONAll compounds were obtained firstly from the plant, and the compounds 10 and 11 were isolated in this genus for the first time.

Dendrobium ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Phenols ; chemistry ; isolation & purification

OBJECTIVETo investigate the chemical constituents of the brown alga Leathesia nana.

METHODCompounds were isolated by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were elucidated with spectroscopic methods including IR, MS and NMR.

RESULTSix compounds were isolated from ethanolic extract of the brown alga L. nana and their structures were identified as 2,2',3,3'-tetrabromo-4,4',5,5'-tetrahydroxydiphenyl methane, 2,2',3-tribromo-3', 4,4', 5-tetrahydroxy-6'-ethyloxymethyldiphenyl methane, 2,3-dibromo-4,5-dihydroxybenzyl alcohol, 2,3-dibromo-4,5-dihydroxybenzyl methyl ether, 3-bromo-4-hydroxybenzoic acid and 2-bromo-4,5-dihydroxybenzaldehyde.

CONCLUSIONAll these compounds were obtained from this species for the first time.

Benzhydryl Compounds ; chemistry ; isolation & purification ; Ethanol ; Phaeophyta ; chemistry ; Phenols ; chemistry ; isolation & purification

Phenolic compounds, the most abundant secondary metabolites in plants, have received more and more attention in recent years because of their distinct bioactivities. This review summarizes different types of phenolic compounds and their extraction and analytical methods used in the recent reports, involving 59 phenolic compounds from 52 kinds of plants. The extraction methods include solid-liquid extraction, ultrasound-assisted extractions, microwave-assisted extractions, supercritical fluid extraction, and other methods. The analysis methods include spectrophotometry, gas chromatography, liquid chromatography, thin-layer chromatography, capillary electrophoresis, and near-infrared spectroscopy. After illustrating the specific conditions of the analytical methods, the advantages and disadvantages of each method are also summarized, pointing out their respective suitability. This review provides valuable reference for identification and/or quantification of phenolic compounds from natural products.
Phenols ; chemistry ; isolation & purification ; Plant Extracts ; chemistry ; isolation & purification ; Plants ; chemistry

OBJECTIVETo study the anti-inflammatory and antioxidant effects of Petasites tricholobus and its phenolic components.

METHODPhenolic compounds were separated purified by column chromatographic methods such as macroporous resin D-101, silica gel, ODS, MCI GEL CHP 20P, Sephadex LH-20. Their structures were identified on the basis of physicochemical property and multiple spectral data.

RESULTNineteen phenolic compounds were separated from 95% ethanol extracts from P. tricholobus Franch. and identified as sulfonated benzyl glucoside (1), 3-(4beta-D-glucopyranosyloxy-3, 5-dimethoxy) -phenyl-2E-propenol (2), dihydrosyringin (3), tangshenosides II (4), 4-hydroxy-2,6-dimethoxyphenol-1-O-beta-D-glucopyranoside (5), 4-hydroxymethyl-2, 6-dimethoxyphenyl-1-O-beta-D-glucopyranoside (6), arbutin (7), rutin (8), kaempferol-3-O-alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranoside (9), quercetin-3-O-beta-D-glucopyranoside (10), kaempferol-3-O-beta-D-glucopyranoside (11), afzelin (12), petasiphenol (13), caffeic acid (14), chlorogenic acid (15), 2-hydroxy-5-acetylbenzoic acid (16), p-hydroxy-benzoic acid (17), protocatechuic aldehyde (18) , and p-hydroxy-phenylpropionic acid (19).

CONCLUSIONAbove result shows that phenolic compounds contained in P. tricholobus mainly include simple phenols, phenolic glycosides, coffee acid and flavonoid glycosides. Among them, compound 1 was separated from the composite family for the first time; compounds 2-7, 9, 11, 12, 16, 19 were separated from the genus Petasites for the first time, and the others were separated from the plant for the first time. These compounds have been proved to have pharmacological effects such as anti-inflammation, antibiosis, antioxidantion.

Anti-Inflammatory Agents ; analysis ; isolation & purification ; Antioxidants ; analysis ; isolation & purification ; Asteraceae ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Phenols ; analysis ; isolation & purification

OBJECTIVETo study the chemical constituents of the roots of Stellera chamaejasme.

METHODThe chemical constituents were separated and purified by chromatographic method after solvent extraction and were identified by spectroscopic analysis.

RESULTTwo phenolic compounds were obtained and determined as stelleranol (1) and umbelliferone-7-O-glucoside (2).

CONCLUSIONCompound 1 was a new compound, and compound 2 was isolated from this plant for the first time.

Magnetic Resonance Spectroscopy ; Molecular Structure ; Phenols ; chemistry ; isolation & purification ; Thymelaeaceae ; chemistry

OBJECTIVETo study the chemical constituents from the roots of Distylium myricoides.

METHODThe chemical constituents were isolated and separated with chromatographic techniques, and structures were identified by spectroscopic methods.

RESULTEight phenolic compounds were isolated and identified as: 4-hydroxy-2-methoxyphenol 1-O-beta-D-(6'-O-galloyl) glucopyranoside (1), 4-hydroxy-3-methoxy-phenol 1-O-beta-D-(6'-O-galloyl) glucopyranoside (2), 3,4,5-tri methoxyphenol 1-O-beta-D-(6'-O-galloyl) glucopyranoside (3), 1,2,3,4,6-penta-O-galloyl-beta-D-glucopyranoside (4), methyl gallate (5), ethyl gallate (6), 3,4-dihydroxybenzoic acid (7), gallic acid (8).

CONCLUSIONAll compounds were isolated from the genus Distylium for the first time.

Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Hamamelidaceae ; chemistry ; Phenols ; chemistry ; Plant Roots ; chemistry

This research was carried out to study the secondary metabolites of endophytic fungus Aspergillosis fumigatus from Euphorbia royleana. The endophytic fungus A. fumigatus was fermented by solid fermentation,and purified by various chromatographic methods after extraction. The structures of the compounds were identified by1 H-NMR,13 C-NMR and HSQC,HMBC spectra and physicchemical properties. Three compounds were isolated and their structures were identified as 3-( 3,4-dihydroxybenzoyl)-5-( 3,4-dihydroxyphenyl)-6-methyl-5,6-dihydro-2 H-pyran-2-one( 1),hydroxysydonic acid( 2) and 11-hydroxysydonic acid( 3). Compound 1 is a new compound.
Aspergillus fumigatus/chemistry* ; Endophytes/chemistry* ; Euphorbia/microbiology* ; Fermentation ; Phenols/isolation & purification*

OBJECTIVETo study the compounds from the whole plant of Galium aparine var. tenerum.

METHODThe various column chromatographic techniques were applied to isolate the chemical constituents. MS, NMR and 2D-NMR spectroscopic techniques were uesd to identify chemical structures.

RESULTSix compounds were isolated from the EtOAc-soluble part of the 95% ethanol extract of the plant, and their chemical structures were identified as 1-(4-hydroxyphenyl)-ethanone (1), vanillic acid (2), 3,4-dihydroxybenzoic acid (3), p-hydroxycinnamic acid (4), gallic acid (5), 4-hydroxytruxillic acid (6).

CONCLUSIONThe NMR data of compound 6 were completely assigned by 2D-NMR techniques, including 1H-1H COSY, HMQC and HMBC spectra. This was the first time to report isolation of the compound 6 from natural product. The compounds 1-5 was isolated from the genus Galium for the first time.

Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Galium ; chemistry ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Phenols ; chemistry ; isolation & purification