Species Da cam was identified as being Hedyotis capitellata Wall. Ex G.Don var. molis Piere ex Pit. It is used as a drug in traditional medicine. Primary results on our studies of Da cam have been presented that: the of Da cam contained alkaloid, saponin, iridoid, tanin. We have extracted and isolated from the leaves caulis of Da cam Sapogenin KLD3 and determinated it was Olanaolic acid
chemistry ; Hedyotis ; Pharmaceutical Preparations

Basing on common chemical reactions, thin layer chromato graphy, absouptive ultraviolet spectrum some parameters were determined for preparation containing benzodiazepine in HCM City. The efficiency of extraction of benzodiazepan in 6 self made sample: Rhino sweet drink, coca-cola, combined food, soya milk, 333 beer and urine. Results found 17 active substances of diazepine group in 131 specialities of commercial propriatary name in HCM City, where diazepam had accounted higher rate in narcotic intoxixation cases. In criminal cases involved in tranquillisant narcotics, the most common substance were diazepan, flunitrazepan, clonazepan, bromazepan, alprazolam, clorazepat, oxazepan and nor-diazepan
Benzodiazepines ; Pharmaceutical Preparations ; Chemistry

143 bacterial strains isolated in Vietnam for the cephalosporin C acylase activity. Among them, 116 strains showed growth on the LB plates containing cephalosporin and 28 strains did not grow. From 7 strains which grew very strongly on the LB plates containing cephalosporin C, 6 strains designated as 71, 91, DT3, M6, 03 and Tb23 were selected for further cultivation in the liquid culture medium and for determination of CA activity. The cell growth curves of these strain were very similar. The cell density reached a maximum at 24-30 hours of cultivation. Among three strains 71, DT3 and Tb23 evaluated for the CA activity, DT3 showed the highest CA activity. Thus, the strain DT3 was identified as Serratia marcescens sp. DT3. This is the first report about the Serratia strain producing CA enzyme
Pharmaceutical Preparations ; pharmacy ; chemistry

The chemical composition of Rhizoma Polygonati (RP) after processing was investigated. The result: All 4 process methods also procedure products no causing itch, black, sweet, soft, plastic. The chemical composition of unrefined Rhizoma Polygonati contained Alcaloid, Flavonoid, Sterol, Sugar, Iridoid glycosid, Amino acid and Fat. On the thin layer chromatograph, total alcaloid of Rhizoma Polygonati processed by a way had 4 spots, total flanoid had 4 spots, 1 spots of them cleare blue. The total sugar content of Rhizoma Polygonati processed by a way is 33,4% and of unrefined Rhizoma Polygonati is 12,63%. Both unrefined RP and processed RP also have 17 acid amin, among 7 essential acid amin for the body. The content of this acid amin of processed sample is usually higher than of unrefined sample
Chemistry ; Pharmacology ; Pharmaceutical Preparations

The root of Hedyotis capitellata Wall. ex G.Don var. mollis Piere ex Pit. Rubiaceae, has been investigated chemical constituents. The roots contained alkaloids, saponin, tannin, anthranoid. Of these, 3 compounds were purified and their IR,UV, EIMS, NMR spectra were determined and showed that: They were Hedyocapitine, Capitelline and Di- (2’- ethyl-1’-hexyl) phthalate (or1,2- benzenedicarboxylic acid, Di- (2’-ethyl-1’-hexyl) ester).
Chemistry ; Plant Roots ; Pharmaceutical Preparations

The leaves of Blumea balsamifera DC Ha Giang was studied. The content of oils was determined by steam distiling method with improved oils quantitative instrument at Medicine Department of Ha Noi College of Pharmacy. The oils was analyzed by GC/MS. The result: The oils from leaves of Blumea balsamifera DC Ha Giang includes 29 components. The main components are: borneol 57,82%, beta-caryophylen 8,27%, denta-cadiol 7,95%, carryophylen oxid 3,1%, patchoulen 2,98%, veridiflorol 2,54% and 23 other components with content approximately 1%. Camphor only exist in the oils with content 1,12%. At Ha noi, 29 components and 18 components at Kak Mil was determined
Chemistry ; Medicine, Traditional ; Pharmaceutical Preparations

The initial investigation of pesticides using cultivation of medical plants in ThietTru, BinhMinh, KhoaiChau, HungYen was reported. Almost pesticides in common use for cultivation of medicinal plants there were recorded and classified. The results of investigation showed that they were often used extensively and in a wrong way. although the Organo-chlorine pesticides (except endosulfan) were no longer in use but at least one prohibited pesticide (methamidophos) were still illegally overused. Further investigation in broader areas for some other localities should be undertaken for systematical evaluation of pesticides use in the cultivation of medicinal plants, and especially its impact. Residual pesticides in herbal medicines should be determined as well, since then, some active measure of management and method of control will be proposed in order to strengthen the quality assurance of this precious resource of medicines.
chemistry ; Pharmaceutical Preparations ; Plants, Medicinal ; Medicine, Traditional

OBJECTIVETo compare the contents of five anthraquinone components in five different processed products from Rheum palamatum.

METHODThe contents of aleo-emodin, rhein, emodin, chrysophanol and physcion were determined simultaneously by HPLC on plogilent TC-C18 (2) column at 35 degrees C with the methanol-0.1% phosphoric acid (85: 15). The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the five components was better over 0.999 9 and the average recoveries were 96.44%, 98.11%, 99.30%, 98.00% and 97.86%, respectively.

CONCLUSIONThe results showed the remarkable variation regulations show in the five different processed products. Compared to the no-parched pieces, the contents of the five anthraquinone components have evidently increased in the braising with liquor and the charring products, and reduced in the vinegar and the liquor sauted pieces.

In this paper, the inline turbidity sensor technology was used to quantify the turbidity of the solution during the dissolution of Chinese medicine granules. The probe measurement position and the magnetic stirring speed were optimized. As a result, the stirring speed was 400 r·min~(-1), and the probe position was at 1/4 of the diameter of the beaker. The measurement results were accurate and reliable. Totally 105 batches of commercially available Chinese medicine granules were collected and dissolved according to the requirements of the Chinese Pharmacopoeia. At the time point of 5 min, 57 batches of granules were completely dissolved, and the corresponding turbidity values ranged between 0-70 FTU; 32 batches of granules showed a slight turbidity, and the corresponding turbidity values ranged between 70-350 FTU; 14 batches of granule solution were turbid, and the corresponding turbidity values ranged between 350-2 000 FTU; two batches of granule solution were heavily turbid, and the corresponding turbidity values were >2 000 FTU. Among the above results, the number of batches in line with the pharmacopoeia dissolution requirement was 84.76%, and the dissolution of some granules still needed to be improved. The turbidity sensor recorded the change curve of turbidity value over time(solubility behavior curve). The degree of important of disintegration and dissolution during the dissolution process showed disintegration > dissolution, disintegration≈dissolution, disintegration < dissolution. The dissolution behavior of the granules can be classified into three categories. The analysis of the mechanism in the process of granule solubility provides a basis for product process improvement.
Medicine, Chinese Traditional ; Pharmaceutical Preparations/chemistry* ; Solubility ; Tablets ; Technology, Pharmaceutical

AIMTo investigate the interaction between drugs and ordered phospholipid membrane using immobilized artificial membrane chromatography (IAMC).

METHODSIAMC was used to determine the interaction drugs with phospholipid membrane, expressed as membrane affinity (lg kIAM). An n-octanol/buffer system was also employed as the reference hydrophobicity (lg Do/w,7.4).

RESULTSWithin the range of used acetonitrile percentages (phi) 0-30% in mobile phase, retention index (lg kIAM) showed excellent correlation with phi. Intercepts of fitted straight lines between lg kIAM and phi were comparable but slopes were much different for the three organic modifiers (acetonitrile, ethanol and methanol). Effects by adding CH2 substituent on lipophilicity difference (delta lg kIAM and delta lg Do/w,7.4) were similar for p-hydroxyl benzoic methyl ester to butyl ester, whereas different for p-hydroxylbenzoic acid to methyl ester.

CONCLUSIONIAMC system is a convenient, efficient and rapid tool for determining membrane interaction.