1.Primary research on vertical HIV-1 transmission in utero from mother-to-infant in Ho Chi Minh City
Journal of Medical Research 2005;33(1):5-11
The vertical HIV transmission from mother to infant can occur in the pregnant period (in utero), during labor or after delivery from breastfeeding. It’s difficult to diagnose HIV-infected newborns by means of serology test, since IgG antibody can infiltrate through placenta from HIV-infected mothers and therefore the serology test is always positive in their babies, even in case the babies were not HIV-infected. In this study, the vertical mother-to-infant HIV-1 transmission in utero was detected by using polymerase chain reaction (PCR) with p17, c2v3 (gp120) and gp41 primers. 37 of 37 (100%) mother blood specimens and 2 of 37 (5.4%) their newborn umbilical cord blood samples were HIV-1 PCR positive. The PCR success rate with P17 and gp41 primers was much higher than that of PCR with c2v3 primer, especially on umbilical cord blood samples. It is recommended that a PCR test using the least 2 primers (p17 and gp41) should be performed at birth to detect HIV-1 seropositive infants and to allow the initiation of antiretroviral therapy. It is necessary to follow up baby health and perform repeatedly HIV-1 PCR on their blood samples to diagnose the timing of HIV-1 transmission.
HIV
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Pregnant Women
2.The expression of HLA class I on the cells of Nasopharyngeal Carcinoma (NPC) tissue
Journal of Vietnamese Medicine 2004;295(2):6-10
15 patients with NPC were diagnosed by pathology were undifferentated carcinoma. The authors used the indirected immuno-fluorescent technique with the monoclonal antibody to determine HLA class I at squarmous cell of NPC tissue. HLA class I expressed weakly: 2112.8% Pan ABC; 10.838.37% B2M. There was a correlation between the prognosis groups and expressing of HLA class I
Nasopharyngeal Neoplasms
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Carcinoma
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Diagnosis
3.Amount of peripheral B and T lymphocytes in healthy medical students
Journal of Vietnamese Medicine 2004;295(2):36-39
Study on 160 the third students of Ha Noi Medical College (85 males, 75 females), the age from 19 to 25. The B and T lymphocytes play an important role in humoral as well as cellar immuno response. Study on the amount of peripheral B and T lymphocytes is useful to assess the biological features in Vietnamese. The amount of lymphocytes with CD4, CD8, CD3, CD19 and the ratio of CD4/CD8, CD4/CD3, CD8/CD3, were determined by FACS-Count and indirected immuno fluorescent technique
Lymphocytes
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Students, Medical
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Cells
4.Study of the mutation of the CCR5 and SDF1 gene in the HIV-1 infected mothers and their children
Anh Thi Thu Phan ; Thuy Thanh Nguyen ; Lan Thi Phuong Nguyen
Journal of Medical Research 2007;47(2):16-22
Background: HIV (human immunodeficiency virus) is the virus that causes AIDS. This virus is passed from one person to another through blood-to-blood and sexual contact. In addition, infected pregnant women can pass HIV to their baby during pregnancy or delivery, as well as through breast-feeding. Objectives:To study the CCR5- 32 and SDF 1-3 A allelic frequence in the HIV -1 infected mothers and their children. Subjects and method: Amplificated on CCR5 and SDF1 gene by PCR and restriction of this fragment length polymorphisme (RLFP) assay for detection of the mutated gene by EcoR1 and Hpall. Results: No mutation of CCR5 was found but only mutation identified at the SDF1 gene. Mutation identified at the SDF1 gene of the mother was: homozygote 2.7% (accounted for 2/37 cases), heterozygote 40.54% (accounted for 15/37 cases) and at the children: homozygote 5.4% (accounted for 1/37 cases), heterozygote 45.95% (accounted for 17/37 cases). The CCR5 chemokin receptor is a co-receptor for M trofic HIV-1 strains, which predominate in the early stage of the HIV disease and SDF-1 natural ligand for the CXCR4 reception. The mutation of these genes protect from HIV-1 infection (slow progression).\r\n', u'Conclusion: It\u2019s necessary to find the mutation of CCR5 and CCR2b related the progression of HIV patients. \r\n', u'
HIV-1/ metabolism
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Receptors
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CCR5
5.Molecular and Clinicopathological Features of Gastrointestinal Stromal Tumors in Vietnamese Patients
Quoc Dat NGO ; Quoc Thang PHAM ; Dang Anh Thu PHAN ; Anh Vu HOANG ; Thi Ngoc Ha HUA ; Sao Trung NGUYEN
Journal of Pathology and Translational Medicine 2019;53(6):361-368
BACKGROUND: Gastrointestinal stromal tumors (GISTs) are the most frequent mesenchymal neoplasms of the gastrointestinal tract. Management of GIST patients is currently based on clinicopathological features and associated genetic changes. However, the detailed characteristics and molecular genetic features of GISTs have not yet been described in the Vietnamese population.METHODS: We first identified 155 patients with primary GIST who underwent surgery with primary curative intent between 2011 and 2014 at University Medical Center at Ho Chi Minh City, Vietnam. We evaluated the clinicopathological features and immunohistochemical reactivity to p53 and Ki-67 in these patients. Additionally, KIT genotyping was performed in 100 cases.RESULTS: The largest proportion of GISTs was classified as high-risk (43.2%). Of the 155 GISTs, 52 (33.5%) were positive for Ki-67, and 58 (37.4%) were positive for p53. The expression of Ki-67 and p53 were correlated with mitotic rate, tumor size, risk assessment, and tumor stage. Out of 100 GIST cases, KIT mutation was found in 68%, of which 62 (91.2%) were found in exon 11, two (2.9%) in exon 9, and four (5.8%) in exon 17. No mutation in exon 13 was identified. Additionally, KIT mutations did not correlate with any clinicopathological features.CONCLUSIONS: The expression of Ki-67 and p53 were associated with high-risk tumors. Mutations in exon 11 were the most commonly found, followed by exon 17 and exon 9. Additionally, KIT mutation status was not correlated with any recognized clinicopathological features.
Academic Medical Centers
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Asian Continental Ancestry Group
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Exons
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Gastrointestinal Stromal Tumors
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Gastrointestinal Tract
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Humans
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Molecular Biology
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Risk Assessment
;
Vietnam
6.Mental Health Among Healthcare Workers During the COVID-19 Pandemic in Vietnam
Nhan Phuc Thanh NGUYEN ; Ha Phan Ai NGUYEN ; Cao Khoa DANG ; Minh Tri PHAN ; Huynh Ho Ngoc QUYNH ; Van Tuan LE ; Chinh Van DANG ; Tinh Huu HO ; Van Trong PHAN ; Thang Van DINH ; Thang PHAN ; Thi Anh Thu DANG
Journal of Preventive Medicine and Public Health 2024;57(1):37-46
Objectives:
The objective of this study was to characterize mental health issues among Vietnamese healthcare workers (HCWs) and to identify related factors.
Methods:
A cross-sectional study was conducted with 990 HCWs in 2021. Their mental health status was measured using the Depression, Anxiety, and Stress Scale.
Results:
In total, 49.9%, 52.3%, and 29.8% of respondents were found to have depression, anxiety, and stress, respectively. The multivariable linear regression model revealed that factors associated with increased anxiety scores included depression scores (β, 0.45; 95% confidence interval [CI], 0.39 to 0.51) and stress scores (β, 0.46; 95% CI, 0.41 to 0.52). Factors associated with increased depression scores included being frontline HCWs (β, 0.57; 95% CI, 0.10 to 1.10), stress scores (β, 0.50; 95% CI, 0.45 to 0.56), and anxiety scores (β, 0.41; 95% CI, 0.36 to 0.47), while working experience was associated with reduced depression scores (β, -0.08; 95% CI, -0.16 to -0.01). Factors associated with increased stress scores included working experience (β, 0.08; 95% CI, 0.00 to 0.16), personal protective equipment interference with daily activities (β, 0.55; 95% CI, 0.07 to 1.00), depression scores (β, 0.54; 95% CI, 0.48 to 0.59), and anxiety scores (β, 0.45; 95% CI, 0.39 to 0.50), while age was associated with reduced stress scores (β, -0.12; 95% CI, -0.20 to -0.05).
Conclusions
Specific interventions are necessary to enhance and promote the mental health of HCWs so they can successfully cope with the circumstances of the pandemic.
7.Identification of Enterovirus C105 for the first time in New Zealand
Cong Thanh Duong ; Tran Hien Nguyen ; Anh Tuan Nguyen ; Thi Thanh Ha Hoang ; Hong Thang Pham ; Thi THanh Ha Nguyen ; Anh Tuan Le ; Dai Quang Tran ; Hong Tram Tran ; Le Hai Nguyen ; Thi Thu Huong Phan ; Hai Son Vo ; Hoang Duc Bui ; Thien Nga Nguyen ; David Jacka ; Keith Sabin
Western Pacific Surveillance and Response 2015;6(1):52-54
In this report we describe a new approach in HIV sentinel surveillance that was piloted in Viet Nam in 2009 and is currently being rolled out in all provinces. It comprises a brief behavioural questionnaire added to the HIV sentinel surveillance surveys conducted routinely among people who inject drugs, female sex workers and men who have sex with men. Timely reporting of data from this system has resulted in improvements to HIV prevention efforts for most at-risk populations.
8.HER2 Status and Its Heterogeneity in Gastric Carcinoma of Vietnamese Patient.
Dang Anh Thu PHAN ; Vu Thien NGUYEN ; Thi Ngoc Ha HUA ; Quoc Dat NGO ; Thi Phuong Thao DOAN ; Sao Trung NGUYEN ; Anh Tu THAI ; Van Thanh NGUYEN
Journal of Pathology and Translational Medicine 2017;51(4):396-402
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) is related to the pathogenesis and poor outcome of numerous types of carcinomas, including gastric carcinoma. Gastric cancer patients with HER2 positivity have become potential candidates for targeted therapy with trastuzumab. METHODS: We investigated 208 gastric cancer specimens using immunohistochemistry (IHC), fluorescence in situ hybridization and dual in situ hybridization (ISH). We also investigated the concordance between IHC and ISH. The correlation between HER2 status and various clinicopathological findings was also investigated. RESULTS: In total, 15.9% (33/208) and 24.5% (51/208) of gastric cancers showed HER2 gene amplification and protein overexpression, respectively. A high level of concordance between ISH and IHC analyses (91.3%, κ = 0.76) was found. A significant correlation between HER2 status and intestinal-type (p < .05) and differentiated carcinomas (p < .05) was also noted. The HER2 heterogeneity was high in gastric cancers; we found 68.8% phenotypic heterogeneity and 57.6% genotypic heterogeneity. Heterogeneity in HER2 protein expression and gene amplification showed a close association with diffuse histologic type and IHC 2+. CONCLUSIONS: HER2 protein overexpression and gene amplification were detected in 24.5% and 15.9% of gastric cancer specimens, respectively. Intestinal-type showed a higher level of HER2 protein overexpression and gene amplification than diffuse type. HER2 status also showed a significant relationship with well- and moderately-differentiated carcinomas. The ratio of phenotypic and genotypic heterogeneity of HER2 was high in gastric carcinomas and was associated with HER2 IHC 2+ and diffuse histologic type.
Asian Continental Ancestry Group*
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Fluorescence
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Gene Amplification
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Genes, erbB-2
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Humans
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Immunohistochemistry
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In Situ Hybridization
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Population Characteristics*
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Receptor, Epidermal Growth Factor
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Stomach Neoplasms
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Trastuzumab
9.Immunohistochemical expression in idiopathic inflammatory myopathies at a single center in Vietnam
Dat Quoc NGO ; Si Tri LE ; Khanh Hoang Phuong PHAN ; Thao Thi Phuong DOAN ; Linh Ngoc Khanh NGUYEN ; Minh Hoang DANG ; Thien Thanh LY ; Thu Dang Anh PHAN
Journal of Pathology and Translational Medicine 2024;58(4):174-181
Background:
The identification of idiopathic inflammatory myopathies (IIMs) requires a comprehensive analysis involving clinical manifestations and histological findings. This study aims to provide insights into the histopathological and immunohistochemical aspects of IIMs.
Methods:
This retrospective case series involved 56 patients diagnosed with IIMs at the Department of Pathology, University of Medicine and Pharmacy at Ho Chi Minh City, from 2019 to 2023. The histology and immunohistochemical expression of HLA-ABC, HLA-DR, C5b-9, Mx1/2/3, and p62 were detected.
Results:
We examined six categories of inflammatory myopathy, including immunemediated necrotizing myopathy (58.9%), dermatomyositis (DM; 23.2%), overlap myositis (8.9%), antisynthetase syndrome (5.4%), inclusion body myositis (IBM; 1.8%), and polymyositis (1.8%). The average age of the patients was 49.7 ± 16.1 years, with a female-to-male ratio of 3:1. Inflammatory cell infiltration in the endomysium was present in 62.5% of cases, perifascicular atrophy was found in 17.8%, and fiber necrosis was observed in 42 cases (75.0%). Rimmed vacuoles were present in 100% of cases in the IBM group. Immunohistochemistry showed the following positivity rates: HLA-ABC (89.2%), HLA-DR (19.6%), C5b-9 (57.1%), and Mx1/2/3 (10.7%). Mx1/2/3 expression was high in DM cases. p62 vacuole deposits were noted in the IBM case. The combination of membrane attack complex and major histocompatibility complex I helped detect IIMs in 96% of cases.
Conclusions
The diagnosis of IIMs and their subtypes should be based on clinical features and histopathological characteristics. Immunohistochemistry plays a crucial role in the diagnosis and differentiation of these subgroups.