Background: Salpingostomy for ectopic pregnancy in patients who has aspirations to having a baby is a necessity. Objective: To discover the factors affecting the outcome of salpingostomy. Subjects and method: 400 patients with none or one baby treated by salpingectomy or salpingostomy for ectopic pregnancy in National Hospital of Obstetrics and Gynecology from July 2006 to June 2007 were studied. The data was analyzed with T test and logistic regression. Results: 92 of 400 patients (23%) were treated by salpingostomy. The risk of salpingectomy for patients with size of pregnancy mass >2 cm (measured by ultrasound) was 2.48 times higher than that of mass <=2 cm (95% CI: 1.50-4.12). All of cases with positive fetal heart beat were treated by salpingectomy. The danger of salpingectomy for patients with preoperative level of bhCG >3000 UI/L increased by 6.65 fold in comparison with that of bhCG <=3000 UI/L (95% CI: 2.99-15.27). The risk of salpingostomy for patients with the size of pregnancy mass >3 cm is 7.43 times as much as that of mass <=3 cm (95% CI: 3.89-14.39). Conclusion: The chance of salpingostomy for patients having the size of pregnancy mass <=3 cm and bhCG <=3000 UI/L was 24.1%.
Ectopic pregnancy ; salpingostomy

Background: Peripheral blood stem cells are today the mains source of cells for transplantation. The number of CD34 in peripheral blood of adult healthy volunteers is very low, so we have to mobilize. Objectives: To apply a protocol to collect CD4 cells in peripheral blood of normal donors mobilized by using G-CSF. Subjects and method: G-CSF (Leukokin) at a dose 10 mcg/kg/day was injected subcutaneously in 5 consecutive days for mobilization CD34 from 5 healthy donors. Count the number of CD34 everyday and collect CD34 cells in 5th \ufffd?6th day by automated blood cell separator (COBE-Spectra), observed clinical and hematilogy, biochemistry symtoms spontaneously. Results: The number of CD34 harvested is 215,5 \ufffd?570,2 x 106 CD34/donor. Almost other of parameters of hematology and biochemistry of donors were normal after one week from the last separation. Conclusion: Mobilization with G-CSF and aphaeresis of periphral blood stem cell from normal donors is feasibility and safety. The number of CD34 can be to allograft for an adult patient.
Hematopoietic Stem Cells ; Granulocyte Colony-Stimulating Factor ;

Background: Massive blood transfusion is common in surgery: trauma, complicated surgery, organ implantation. Stored blood contains much lactate, ammonia, products of metabolism. These products have negative effects on patient\u2019s metabolic function which may be a cause of death. Objective: To study the change of blood biochemical indexes in surgical patients with massive blood transfusion at Viet Duc hospital. Subjects and method: A prospective study was conducted in the adult patients who had emergency and operation at Viet Duc hospital, from December/2004 to August/2006. All of them were transfused over 3000 ml of blood within 24 hours (pre-operation, intra-operation and post-operation). Results: During the period of study, there were 70 surgical patients with massive blood transfusion: men were more than woman (64%) and the mean age: 38\xb117. The survival rate was significantly improved (57%). The average transfused blood per patient per 24 hours was 3995ml\xb11067ml. There was a decrease in the value of pH, acid-base and HCO3 (the average pH value 7.218\xb10.181, lowest value 6.7). The average value of pH and HCO3\xac was higher in the survival patients than died patients (p<0.05). The mean level of K+ was 3.8\xb10.96mml/l. The increase in the level of K+ had a positive association with the amount of transfused blood. The mean level of Ca++ \xac\xacdecreased under the normal level (0.98mml). Conclusion: It should monitor blood gas and electrolytes continuously in surgical patients with massive blood transfusion in order to identify disorders soon for timely treatment.
Blood Transfusion

Background: Virus is one of main causes of children acute encephalitis syndrome in countries of Asia south-east. In Vietnam, apart from Japanese Encephalitis Virus which is considered as main cause of children acute encephalitis syndrome, there are other viral pathologies, of which is Nam Dinh virus. Nam Dinh virus \ufffd?a novel Arbovirus was isolated from acute encephalitis syndrome patient in northern Vietnam, 2002. The circulation of this virus has been recognized in the north, central, and highland regions. Objective: To observe the multiplication of Nam Dinh virus on the Aedes albopictus cell line clone C6/36. Materials and method: In this study the ultra-thin section method was used to observe the multiplication of the Nam Dinh virus on the Aedes albopictus cell line clone C6/36, 48 hours post-infection. Results and Conclusion: Nam Dinh encephalitis virus got used to Aedes albopictus cell line clone C6/36 and damaged cells, 24-48 hours post-infection. Its multiplication is taking place in the cytoplasm, a typical characteristic of RNA virus. Nucleocapsids of the virus were found in vacuoles of the cell. Proteincapsid of the virus was synthesized in a rough endoplasmic reticulum (rER). After assembling in the cytoplasm, the virus is released from the cell by budding and used the cell membrane as its envelope.
Viruses/growth & ; development ; Encephalitis ;

Background: Umbilical cord blood - Stem cell CD34 are attracted to study and apply to cure many serious diseases. But the quantity of the cell is small, so it only used to children. Therefore, studying to find out the high effect collected process is necessary. Objective: studying to find out the high effect process of collecting hematopoietic stem cell from umbilical cord blood. Subjects and methods: the study included 112 umbilical cord blood specimens collected from healthy mother delivering at National Hospital of Obstetric and Gynecology.Results: The condition of the mother was healthy, with no risk factors of infection or genetic disease. The average age of mothers was 28 \xb1 5 years old, average weight: 63 \xb1 5 kg. Age of fetus was from 39-41 weeks, average weight: \ufffd?3000g. Collection technique: cutting the umbilical cord after delivery 6-10 seconds, cutting the umbilical cord far from neonatal 6 cm, blood collection continued after the collection of placenta, all processes must be implemented rapidly in the 4 -5 minutes in aseptic condition. With the above process it can be obtained with high effect blood: volume was 90 - 120ml (48%). CD34 cell count: 1.6 \xb1 0.3x106, the number of eukaryotic cells: 13.53 \xb1 1.7x108/unit of the umbilical cord. Conclusions: The process can take the large volume of umbilical cord blood, which can be used for children and low weight adult. \r\n', u'\r\n', u'\r\n', u'
Fetal Blood

Background: Fragile X Syndrome (FXS) is the most common cause of inherited mental retardation. The absence of Fragile X Mental Retardation (FMRP) in Fragile X syndrome changes other proteins. Objective: To detect changes of glycoprotein in human serum of Fragile X syndrome. Subject and methods: Affinity chromatography with lectin concanavalin A (ConA) used to receive glycoprotein. The collected glycoprotein was then separated using 2-D electrophoresis. The protein spots were further excised, trypsin digested, and analyzed by nano LC couple with ESI-MS/MS and identified by MASCOT v1.8 software. Results and conclusion: 5 glycoproteins showed the different expression levels in the serum of Fragile X syndrome. Haptoglobin, Ig-J were increased and ceruloplasmin, transferring, Ig kappa were decreased. Using affinity chromatography with lectin concanavalin A (ConA), glycoprotein was received and divided on 2 ways electrokinetic chromatography. The mixture protein was identified with a reliability of 99.5% by 2 ways liquid chromatography combined with continuous spectrum mass.
Fragile X syndrome ; Fragile X Mental Retardation ; proteomics

Background: HIV (human immunodeficiency virus) is the virus that causes AIDS. This virus is passed from one person to another through blood-to-blood and sexual contact. In addition, infected pregnant women can pass HIV to their baby during pregnancy or delivery, as well as through breast-feeding. Objectives:To study the CCR5- 32 and SDF 1-3 A allelic frequence in the HIV -1 infected mothers and their children. Subjects and method: Amplificated on CCR5 and SDF1 gene by PCR and restriction of this fragment length polymorphisme (RLFP) assay for detection of the mutated gene by EcoR1 and Hpall. Results: No mutation of CCR5 was found but only mutation identified at the SDF1 gene. Mutation identified at the SDF1 gene of the mother was: homozygote 2.7% (accounted for 2/37 cases), heterozygote 40.54% (accounted for 15/37 cases) and at the children: homozygote 5.4% (accounted for 1/37 cases), heterozygote 45.95% (accounted for 17/37 cases). The CCR5 chemokin receptor is a co-receptor for M trofic HIV-1 strains, which predominate in the early stage of the HIV disease and SDF-1 natural ligand for the CXCR4 reception. The mutation of these genes protect from HIV-1 infection (slow progression).\r\n', u'Conclusion: It\u2019s necessary to find the mutation of CCR5 and CCR2b related the progression of HIV patients. \r\n', u'
HIV-1/ metabolism ; Receptors ; CCR5

Background: The change of blood pressure during hemodialysis has been noted for long time. However, there were few studies on the rise of blood pressure during hemodialysis. The clinical meaning of hypertension during hemodialysis has not been understood clearly. Objective: To study the role of calcium concentration in dialysate in the rise of blood pressure during hemodialysis sessions. Subjects and method: Prospective study performed on 9 stable patients on chronic hemodialysis treated at Viet Duc Hospital including 5 female and 4 male patients. The mean age of patients was 47.6 years. The patients had period 1 of 10 weeks of treatment using dialysate 1 A (with calcium concentration 1.8 mmol/l) and then they were switched to period 2 of 10 other weeks using dialysate 3A (with calcium concentration 1.25 mmol/l). Results:The blood pressure of patients during the period 2 using 3A dialysate was better controlled during hemodialysis sessions. The response to erythropoietin treatment was similar in both periods. The serum calcium was lower after using 3A dialysate. Conclusions: Using dialysate with lower calcium concentration can be helpful for controlling the hypertension during hemodialysis sessions. The appropriate calcium concentration in dialysate needs to be selected to avoid the hypocalcaemia in chronic hemodialysis patients.
Renal Dialysis ; Hypertension

Background: The reduction of erythrocytes from cord blood is very need for long - term storage of C034 cells for transplantation. Reduced erythrocyte will reduces preservative blood volume, preservatives and freely HST when defrosting, so stem cells are better protected. Objectives: To study selection of the best centrifugal procedure to reduce maximal erythrocytes and lose minimal C034 cells from cord blood. Subjects and methods: 20 blood samples selected from 60 cord blood units was used for this study. The study was carried out through two steps. In the first step, the centrifugal speed was fixed and the centrifugal time was changed.In the second step, the centrifugal time was fixed, the centrifugal speed was changed. From collected results the best appropriate procedure to reduce erythrocytes from cord blood have been selected. Results: The procedure of gradient centrifuge with speed of 500g in 6 minutes isolated> 50% of erythrocytes, kept > 84% of CD34 cells and then centrifuge of 1000 g in 10 minutes reduced about 40% of volume of nuclear cell - suspension. Conclusion: The procedure can use for preparation of stem cell suspension from cord blood to storage in nitrogen liquid. \r\n', u'\r\n', u'
Erythrocytes/ pathology ; Fetal Blood/ chemistry ; drug effects ; immunology