One of the constituents of the oriental traditional pill named Balok was Tuber Stephaniae rotundae, the active principle of which was rotundine. The test solutions used for the analysis of rotundine were standardized after ISO/IEC 17027 and GLP, the equipment was adjusted after ISO/IEC 17025. The proposed HPLC technique was proved to be appropriate and stable for the quantitative determination of rotundine. The examined concentration showed the linear correlation between the rotundine concentration and the pic surface. The reproducibility of the method was high, with RSD = 0.98%. The accuracy rate of the method was 100.01%, with RSD = 2.0%.
Chromatography, High Pressure Liquid ; Berberine Alkaloids

An HPLC method has been established to determine l-tetrahydropalmatine (rotundine) extracted by chloroform from powders of Stephania viridiflavens, S. dielsiana and Stephania sp. (Menispermaceae). The technique was carried out on an RP-18 (250 x 4.0 mm; 5 m) column with a diode array detector or UV detector at  = 283 nm, using a mixture of 0.05 M potassium dihydrogen phosphate, acetonitrile and triethylamine (62.5 : 37.5 : 0.5) adjusted to pH 4.0 by concentrated phosphoric acid as a mobile phase. The HPLC method proved accurate, specise and sensitive. The results show that differences exist in the content of l-tetrahydropalmatine between species.
Chromatography, High Pressure Liquid ; Berberine Alkaloids

54 cases of hydatidiform mole pregnancy were studied at the Department of Gynecology and Obsterics of the Central Hospital from June 2000 to July 2001, in comparing with 124 cases of normal immature delivery of living neonate. A high rate of clinical symptoms were noted including vaginal hemorrhage in the first trimester, a more unterine height than in normal fetal age, anemia, severe vomit, pre-eclampsia, hyperthyroidism, luteino-adenoma cyst of > 5 cm diametre. There is a positive relation between vaginal bleeding, uterine height before the curettage and the state of luteino adenoma cyst with Chorio complication. Related factors of hydatidiform mole pregnancy were nutritional status, occupation, age, history of abortment and water source hygiene.
Diagnosis ; Hydatidiform Mole ; Pregnancy

Aims: The objective of the study was to isolate bacteriophages and conduct a comprehensive analysis of their potential against Xanthomonas oryzae pv. oryzae (Xoo) strains in the Mekong Delta, Vietnam. Methodology and results: Twelve Xoo strains were isolated from rice fields located in the Mekong Delta, Vietnam. Among these strains, three strains Xoo L019, L020 and L024, showed the highest disease index of bacterial blight. Four phages specific to Xoo were isolated from soil, water and leaf samples, and their morphologies were determined. In a test against 12 Xoo strains, phage L541, MLA23 or W41 could infect 10 of the 12 Xoo strains, while phage LBH01 could infect 8 of the 12 Xoo strains. The stability of the phages to pH, organic solvents, UV-A and UV-B was also evaluated. Conclusion, significance and impact of study The initial characterization of the phages indicates their potential as biocontrol agents against bacterial blight in rice. The study is one of the very first studies about Xoo phages in rice in Vietnam.