Study 87 patients with systemic lupus erythematosus (SLE) treated at National Institute of Demato-Venereology. Among serotypes, prevalence of DR2 in patient group was higher (25.3%). This study, similar to previous studies of Southeast Asia and Asia countries, suggested the relationship of SLE and HLA-DR2, and confirmed again DR2 was a main serotype of HLA-DRB1 locus that determined sensitivity of SLE in Southeast Asia and Asia people.
Lupus Erythematosus, Systemic ; HLA-DR Antigens ; Therapeutics

Patients with systemic lupus erythematosus (SLE) diagnosed and treated at National Institute of Demato-Venereology, based on 4 criteria of SLE classifications. In control group, volunteers were taken blood samples to analyse genotypes. Study the relationship of SLE and polymorphism of FcR-coded gene, authors suggested FcRIIIB genotype may be risk factor of SLE, and FcRIIIB genotype isn’t related to occurrence of SLE.
Lupus Erythematosus, Systemic ; Diagnosis ; Therapeutics

Participants of this study were 11 healthy people. Method of amplification for a specific gene fragment in ADN was used. Sample 1 was controlled by PCR-SSO technique in National Laboratory for Histointegration of Saint-Luis Hospital, Paris. Sample 11 was verified by PCR-RFLP in Immunological Laboratory of Department for Microbiology and Immunology, Aichi University, Japan. Result of verification was similar to that of PCR-SSP technique.
Polymerase Chain Reaction ; HLA-DR Antigens

Participants of this study are 11 healthy people. Method of amplification for a specific gene fragment in ADN was used. Sample 1 was controlled by PCR-SSO technique in National Laboratory for Histointegration of Saint-Luis Hospital, Paris. Sample 11 was controlled by PCR-RFLP in Immunological Laboratory of Department for Microbiology and Immunology, Aichi University, Japan. Result of control was similar to that of PCR-SSP technique.
Phenotype ; Polymerase Chain Reaction ; HLA Antigens

69 patients with systemic lupus erythema (SLE) at the Central Institute of Dermatology and Venerology were studied on. The control group included 57 healthy blood donors. The investigation on the existance of 2bp compression phenomenon on exon 29 using PCR technique showed that: C4A deficiency caused by 2bp compression on exon 29 was not detected on these patients of SLE and in control subjects also. There was no relation between the deficiency of the gene encoding C4A caused by 2bp compression on exon 29 and the development of SLE.
Lupus Erythematosus, Systemic/deficiency ; Control Groups ; Tissue Donors

Study on 99 patients with systemic lupus erythematosus (SLE) who were diagnosed and treated at Dermatology hospital, and all patients had surveyed genotype of FC gamma RIIA. The control group consisted of 93 voluntary blood donors who were surveyed genotype of FCRIIA. Genotype of FCRIIA was defined by PCR-SSP method. Results: 4 PCR reactions were used to determine genotype of FCRIIA. The frequency of R/R131, R/H131 and H/H131 in 99 patients occurred in 15 (15.1%), 74 (74.1%) and 10 (10.1%), respectively and in 93 control persons were 11 (11.8%), 50 (53.8%) and 32 (34.4%), respectively. The distribution of frequency of 3 genotype between 2 groups was different with 2 = 16.16 and p<0.01. Genotype of FCRIIA could be the risk factors for the appearance of SLE in Vietnamese people.
Lupus Erythematosus, Systemic ; Diagnosis

Background: Human Heparansulphate interacting protein (hHip) has been shown to participate in biological processes of cells. Several studies indicated that hHip transcript is up regulated in several of cancer tissues including those of thyroid, colon, breast and prostate. Antibody against hHIP is necessary for methods to evaluate protein level of HIP in cancer tissues. Objectives:The aims of study was to induce anti hHIP antibody in rabbit and purify and conserve purified anti hHIP antibody. Subjects and method: The study included 9 adult and healthy rabbits with the weight 2 - 2.5kg. Immunization hHIP peptide-KLH in rabbit. Purify anti hHIP antibody using affinity chromatography. Results: The results shown synthesize hHIP peptide and conjugate it with carrier protein. Sensitive rabbit better meet with hHIP-KLH antibody. The Ig concentration obtained in sensitive rabbit was rather high and equal. Immunization hHIP-KLH successfully in rabbit. Obtainment valuable amount of anti hHIP antibody. Conclusion: Successfully induce and purify anti hHIP antibody from rabbit. Establish a standard protocol for polyclonal antibody against small peptide in rabbit.\r\n', u'\r\n', u'
Carrier Proteins/ administration & ; dosage ; chemistry ; Rabbits

Background: Hemophilia A is a genetic bleeding disorder that results from a deficiency in factor VIII. The prevalence of Hemophilia in Vietnam is rather high (2/34830 people) and Vietnam has high usage demand for factor VIII in the treatment and prevention of the disease. Therefore, it is necessary to study and produce recombinant blood \u2013 coagulation factor WIII. Objective: To clone successfully A1A2 and A3C2 gen fragment encoding factor VIII. Subject and Method: Amplify A1A2 and A3C2 gene fragments by PCR from human cDNA. PCR products were ligated into cloning vector pQE \u2013 30UA. Recombinant plasmids were transformed into E.coli DH5 alpha host strain. Inserted A1A2 and A3C2 gene fragments were checked by PCR and restriction enzymes. Result: Successfully amplifying functional gene fragments encoding factor VIII using specific primers. Conclusion: Obtaining pQE \u2013 30UA vector carrying A1A2 and A3C2 fragments encoding factor VIII. This is the premise result for the next studies on synthesis of recombinant factor WIII and application of genetic therapy.
Hemophilia ; Blood \u2013 coagulation factor VIII

Background: Suicide continues to be a leading cause of death worldwide. Recently, more than 45,000 children in the age group of 10 to 19 years died by suicide, making it the second leading cause of death in the age group of 15 to 19, surpassed only by traffic accidents, tuberculosis, and fighting. Objective: To determine the prevalence of suicidal ideation among adolescents whose parents are separated/divorced; and to explore the factors associated with suicidal ideation and describe adverse experiences among adolescents. Methods: A cross-sectional descriptive study was conducted in 309 adolescents with separated/separated parents in Hue City. Data was collected through direct interviews using a structured questionnaire. Suicidal ideation was defined as the presence of thoughts or plans related to suicide within the last 12 months. Multivariate logistic regression was applied to identify factors associated with suicidal ideation in adolescents with separated/ separated parents. Results: The study found that 15.5% (95% CI:11.7 - 20.1) of adolescents with separated /separated parents reported experiencing suicidal thoughts, in which men accounted 8.4% (95% CI:5.6 – 11.2) and women accounted 7.1% (95% CI:4.5 - 10.6). Several factors were identified as increasing the risk of suicidal ideation, including alcohol use (OR = 3.24; 95% CI:1.42 - 7.42), hyperactivity/inattention (OR = 4.96; 95% CI:1.58 - 15.605), and a poor quality of family relationships (OR = 4.82; 95% CI:1.26 - 18.50). On the contrary, certain factors were found to reduce the risk, including being in the 14-15 age group of 14-15 (OR = 0.26; 95% CI:0.10 - 0.69) and participating in physical activity (OR = 0.44; 95% CI:0.21 – 0.94). Conclusions: The research highlights a significant percentage of adolescents with separated / divided parents who experience suicidal ideation. Therefore, it is imperative for families, schools, and society to develop comprehensive strategies to monitor and address various risky behaviours among students simultaneously.