Panax notoginseng is a perennial Chinese medicinal plant, which has serious continuous cropping obstacles and is prone to a variety of diseases and insect pests during the growth process. At present, the prevention and control of pests and diseases is mainly carried out through chemical pesticides, and the consequent pesticide residues of P. notoginseng have attracted much attention. This study reviewed the types and detection methods of pesticide residues in P. notoginseng from 1981 to 2021, and compared the limits of pesticide residues in P. notoginseng in China and abroad to provide a reference for rational application of pesticides in P. notoginseng and quality control of medicinal materials, thereby promoting the sustainable development of the P. notoginseng industry in China. Currently, there are only 40 published papers on pesticide residues of P. notoginseng, which is indicative of a serious problem of insufficient research. At present, hundreds of pesticide residues in P. notoginseng can be detected simultaneously by using chromatography-tandem mass spectrometry. The pesticides detected have gradually changed from early prohibited ones, such as dichlorodiphenyl trichloroethane(DDT), benzene hexachloride(BHC), and parathion, to low toxic ones(e.g., dimethomorph, procymidone, propicona-zole, and difenoconazole). The dietary risk from pesticide residues in P. notoginseng is low, which would not cause harm to consu-mers. This study concluded that in the future, the development of the quality standard for pesticide residues of P. notoginseng should be actively carried out. To increase the pesticides used in actual production in the quality standard based on the existing ones and to guide farmers to use pesticides scientifically will be the focus of future work.
China ; Panax notoginseng ; Pesticide Residues/analysis* ; Pesticides/analysis* ; Plants, Medicinal

OBJECTIVETo establish a method for the simultaneous determination of 15 pesticides residues in Radix Paeoniae Alba by large volume injection-gas chromatography-mass spectrometry(LVI-GC-MS).

METHODSThe pesticides, including organochlorine pesticides, organophosphorus pesticides and pyrethroid insecticides, were analyzed by LVI-GC-MS using DB-5MS capillary column (30 m X 250 μm, 0.25 μm). The column temperature programming: initial temperature 40 degree for 1 min, with the increasing rate of 20 degree/min to 210 degree for 2 min, then with the increasing rate of 5 degree/min to 280 degree for 22 min. The flow of carrier gas was 1.0 ml/min with the injection volume of 15 μl.

RESULTSThe calibration curves of the pesticides were linear in the specified concentration ranges with correlation coefficients of 0.9937-0.9995. The average recoveries of the pesticides in Radix Paeoniae Alba spiked at two spiked levels ranged from 60.4 % to 106.8 % (for pendimethalin and 4, 4'-DDE those were 53.1 % and 45.2 %) with relative standard deviation(RSD) of 3.6 % to 18.6 % and the detection limits (S/N=3) were in the range of 0.16 μg/kg to 3.59 μg/kg.

CONCLUSIONThe established method for determination of multi-pesticide residue in Radix Paeoniae Alba is rapid, convenient and accurate with high sensitivity and low-cost.

Gas Chromatography-Mass Spectrometry ; methods ; Paeonia ; chemistry ; Pesticide Residues ; analysis

OBJECTIVETo investigate pesticide abuse on fruits and vegetables in Tianjin Area, to detect pesticide residues in fruits and vegetables, to study the methods for reducing pesticide residues in fruits and vegetables.

METHODS(1) A questionnaire on the pesticide application during growing fruit and vegetable was administered to 185 farmers in Tianjin Area. (2) According to the information from the questionnaire survey, fruit and vegetable samples were collected in four seasons around the year and measured for organophosphorus pesticide residues by gas chromatography. (3) Fruit and vegetable samples contained pesticide residue were treated by scald, immersion in 0.15% and 0.30% detergent solution, immersion in pure water, peeling and cutting root and pesticide residues were measured before and after the treatment.

RESULTSThe percentage of pesticide abuse in growing fruit and vegetable was 65.00% in Tianjin area, and 31.60% of the fruits and vegetable samples collected in summer were positive for high toxic organophosphorus pesticides. Significant decrease of pesticide residue in fruit and vegetable was found by scald, immersion in 0.15% and 0.30% detergent solution, as well as peeling and cutting root, and over 80.00% pesticide residue in the samples could be reduced by scald.

CONCLUSIONSIt is necessary to strengthen pesticide abuse control and market surveillance and inspection, in order to reduce the harmful effects of pesticide residue in fruit and vegetables to human health.

Chromatography, Gas ; Fruit ; chemistry ; Pesticide Residues ; analysis ; Vegetables ; chemistry

Measurements were made of the residual level of chlorinated hydrocarbon compound and mercury compound in the tissues of wild birds and herbs in Korea from November 1974 to August 1975. Aldrin was detected in all of the wild birds analyzed. The residue levels of aldrin varied from 0.353ppm to 16.115ppm. Among the tissues analyzed, the feathers contained the highest concentration of aldrin, but chloridane could not be detected in wild birds. The pesticides detected in wild birds were (alpha+beta)-BHC gamma-BHC, delta-BHC, heptachlor, aldrin, TDE and DDT. Dieldrin was detected only in the stomach of eastern dunlin caught at the Nakdong River basin. Residue levels of mercury were measured in all wild birds analyzed. Among tissues analyzed for mercury compound concentration, here also the feathers showed the highest level. The feathers of the eastern dunlin showed a high content of mercury compound which was 76.665 ppm at the highest level. Herbs used as material for oriental remedies were contaminated by chlorinated hydrocarbons which were (alpha+beta)-BHC, gamma-BHC, dieldrin, DDT, heptachlor, TDE, aldrin and epoxide. The insect materials from Cicadae testa, Bombycis corpus, and Scolopendia were much more contaminated by pesticides than plant materials. Herbs cultivated in arable areas were also found to be more contaminated by pesticides than wild ones. Herbs, on the whole, contained lower levels of chlorinated hydrocarbons than wild birds. The incidence of pesticide residues in natural products and in wild birds, however, should be considered as a global environmental pollution problem. The present investigation could contribute as a baseline study for the monitoring of pesticide pollution, its application and dispersal, and the hazard limit for food and human health.
Animal ; Birds/metabolism* ; Human ; Hydrocarbons, Chlorinated/analysis ; Korea ; Mercury/analysis ; Pesticide Residues/analysis* ; Plants/analysis*

The present study counted the frequency of detection technologies and monitoring frequency of pesticide species by frequency analysis based on the 28 258 pieces of data on pesticide content of Chinese medicinal materials in CNKI, calculated the detection rate and exceeding rate of different types of pesticides, and systematically analyzed the pesticide residue pollution of Chinese medicinal materials. The results showed that there were 40 types of pesticides with detection rates higher than 10%, where new pesticides such as organochlorines and nicotine accounted for 55%, and organic phosphorus, pyrethroids, and carbamates accounted for 17.5%, 15.0%, and 12.5%, respectively. Seventeen types of pesticides exceeded the standard to varying degrees, including 12 types(70.59%) with exceeding rates not higher than 5%, four types(23.53%) with exceeding rates in the range of 5%-10%, and one type(5.88%) with an exceeding rate higher than 10%. As revealed by the analysis results of the past five years, the pesticide residue pollution of Chinese medicinal materials showed a downward trend. Compared with the conditions at worst, organochlorines decreased by about 2/3 in detection rate and 47.23% in exceeding rate, carbamates by about 1/2 in detection rate and 10.78% in exceeding rate, organic phosphorus by 3/4 in detection rate and 7.22% in exceeding rate, pyrethroids by 1/2 in detection rate and 11.05% in exceeding rate, and other types by about 1/2 in detection rate but not exceeded the standard. In general, pesticide residues in Chinese medicinal materials and safety have been significantly improved. However, pesticide residues are still important factors affecting the quality and safety of Chinese medicinal materials. It is suggested to further improve the control standards of pesticide residues in Chinese medicinal materials, strengthen the monitoring of pesticides used in practical production, and promote the ecological planting mode to facilitate the high-quality development of the Chinese medicinal material industry.
China ; Hydrocarbons, Chlorinated/analysis* ; Pesticide Residues/analysis* ; Pesticides/analysis* ; Pyrethrins/analysis*

Carbamates ; analysis ; Fruit ; chemistry ; Organophosphorus Compounds ; analysis ; Pesticide Residues ; analysis ; Vegetables ; chemistry

Biocompatible Materials ; analysis ; Chromatography ; methods ; Chromatography, Gas ; methods ; Chromatography, Liquid ; methods ; Paraquat ; analysis ; Pesticide Residues ; analysis

Panax ginseng, a perennial herb, is prone to diseases and insect pests in the growth process, which are primarily prevented and treated by pesticides. However, due to the lack of standardization in the types, frequencies, and doses of pesticides, pesticide residues have become the main exogenous pollutants of P. ginseng. To explore the risk of pesticide residues in P. ginseng, this paper summarized and analyzed the common pesticide residues in P. ginseng, detection techniques, and pesticide residue limit stan-dards based on the published literature in recent years. The results revealed that the main pesticide residues in P. ginseng were organochlorine pesticides, such as tetrachloronitrobenzene, pentachloronitrobenzene, and hexachlorobenzene, and the detection techniques were dominated by gas chromatography(GC), liquid chromatography(LC), or those combined with mass spectrometry(MS). Because of the long half-life and difficulty in degradation, organochlorine pesticides have become the main factor affecting the export of P. ginseng. It is worth mentioning that P. ginseng has been classified as food in Japan, South Korea, the European Union, and other countries, and the standards of pesticide residues and limits are stricter than those in China. The quality and safety of P. ginseng are prerequisites for the efficacy of Chinese medicine and the development of traditional Chinese medicine. The formulation of scientific and effective standards for pesticide application and limits would promote the high-quality development of the P. ginseng industry.
Gas Chromatography-Mass Spectrometry ; Hydrocarbons, Chlorinated/analysis* ; Panax/chemistry* ; Pesticide Residues/analysis* ; Pesticides/analysis*

OBJECTIVETo establish an analysical method for the quantitative determination of 18 kinds of organophosphate pesticide residues in Flos Lonicerae.

METHODThe pesticides in samples were extracted with acetone by Ultrasonic wave Assistant Extraction (UAE), purified by Solid-Phase Extraction (SPE). Using PFPD detecter gas chromatography with HP-1701 column.

RESULT AND CONCLUSIONAll 18 pesticides were separated well in 30 minutes. The detection limits was 4.0-18.0 microg x kg(-1). The range of recovery and RSD were 83.64%-88.65% and 2.8%-6.0%, respectively. And it met the requirements of pesticide multi-residues analysis.

Chromatography, Gas ; methods ; Flowers ; chemistry ; Lonicera ; chemistry ; Organophosphorus Compounds ; analysis ; Pesticide Residues ; analysis ; Plants, Medicinal ; chemistry

OBJECTIVETo establish a gas chromatography method for simultaneous determination of organochlorine and pyrethroid pesticide residues in Viscum coloratum by cloud-point extraction (CPE).

METHODPesticides were extracted with the non-ionic surfactant Triton X-100. The apparatus was gas chromatography with electron capture detector and the separation was performed on an Hp-5 column. The pesticide residues were calculated by external standard method.

RESULTGood linear relation was obtained over the range of 5-500 microg L(-1) for organochlorine and 10-1,000 microg L(-1) for pyrethroid. The limits of detection was 1.5-7.5 microg kg(-1). The average recoveries of organochlorine and pyrethroid were 74.15% -111.6% with corresponding RSD of 4.0% -9.1%.

CONCLUSIONThe sample and rapid method was applied to pesticide residues determination.

Chromatography, Gas ; methods ; Limit of Detection ; Octoxynol ; chemistry ; Pesticide Residues ; analysis ; Plant Extracts ; analysis ; Viscum ; chemistry