In recent years, the soil borne disease of medicinal plants becomes severely during the process of cultivation and directly endangered the production and quality of raw materials used in traditional Chinese medicine (TCM). The chemical pesticides have been constantly used to prevent and control the soil borne disease, but only a few are effective. Meanwhile, the excessive uses of chemical pesticides also lead pesticide residues in TCM, which often exceed limit of the standard, and harm the human health and cause environmental pollution. Therefore, biological control has become a hot research point for its environmental advantages. This paper mainly discussed the mechanisms of different species of microorganisms, which could control the soil borne disease of medicinal plants, from the following aspects: improving host plants' nutrient absorption, the nutrient and space competition with the pathogenic bacteria, changing the morphology and anatomical structure of roots, adjusting the host plants' endogenous hormones, restoring the balance of host rhizosphere soil microecology and activating the host plants' defense system etc. Then put forward the prospect of biocontrol agents in the future.
Medicine, Chinese Traditional ; Pest Control, Biological ; methods ; Plant Diseases ; prevention & control ; Plants, Medicinal ; Soil Microbiology

UNLABELLEDTo study the effects of the integrated pest control techniques on growth of host larvae of Cordyceps sinensis.

METHODThe integrated pest control techniques were compared with conventional techniques to evaluate the effects on growth of host larvae.

RESULT AND CONCLUSIONThe results showed that the techniques had broken the balance of the microbial living in the material, produced effective inhibition on the pests, raised the survival rate and promoted the growth of the host larvae at the same time.

Animals ; Cordyceps ; physiology ; Larva ; growth & development ; microbiology ; Moths ; growth & development ; microbiology ; Pest Control, Biological ; methods

In order to explore the environmental pest management method of Dorysthenes hydropicus, three strains of entomopathogenic nematodes, viz. Heterorhabditis bacteriphora (H06), Steinernema scapterisci (SS), S. carpocapsae (All) were used on larvae of Dorysthenes hydropicus, with treatments of 0, 5 000 and 10 000 nematodes each larva. The result showed that these three strains viz. All, H06 and SS had high lethal effects on the larvae. Lethal rates had dose-effects relationship with inoculation amounts. High dose treatments resulted in high mortalities and led to quick death, especially in the treatment of H06. Treatment of H06 with 10 000 nematodes per larva resulted in 100% mortality after inoculated 4 days. Different strains of these nematodes had various lethal characters, H06 with only one peak mortality, the larvae died quickly after inoculated, while All and SS with 2 peak mortalities, there was a stable stage with low mortality between the 2 peak mortalities. Entomopathogenic nematodes could be used as a hopeful method for controlling of Dorysthenes hydropicus in fields.
Animals ; Larva ; parasitology ; Moths ; growth & development ; parasitology ; physiology ; Nematoda ; physiology ; Pest Control, Biological ; methods

A type of entomopathogenic fungus of soil in Citrus grandis 'tomentosa' production base was isolated and identified with morphological and molecular biological methods, including pathogenesis, spore characteristic and ITS sequence analysis were conducted. The results showed that eighteen entomopathogenic fungi strains were isolated from the Tenebrio molitor infected in the soil samples, which were identified as Metarhizium anisopliae var. anisopliae. Based on results above, we concluded that there was quantity of Metarhizium resources in this area. These provided the useful information for controlling some pests of C. grandis by using these strains of fungus.
Animals ; Citrus ; parasitology ; Metarhizium ; isolation & purification ; physiology ; Pest Control, Biological ; methods ; Soil Microbiology

GAP production of medicinal plants needs diseases' green control during their cultivation processes. In this article, The authors summarized the achievements in cultural control and biological control to crops' diseases, taking the characteristics of medicinal plants into account, we put out the notion of the green control to medicinal plants' diseases, and indicated that the green control for medicinal plants' diseases should combine with cultural control, modem phytopathology methods, biological control and essential pesticides, besides, we introduced some suggestions and the prospect, to provide a reference for green control of medicinal plants' diseases in their GAP production.
Agriculture ; methods ; Pest Control, Biological ; methods ; Plant Diseases ; microbiology ; parasitology ; prevention & control ; Plants, Medicinal ; chemistry ; growth & development ; microbiology ; parasitology

Pasteuria penetrans will build-up faster where there is a high initial nematode density and can suppress root-knot nematode populations in the roots of tomato plants. The effect of different initial densities of nematode (Meloidogyne javanica) (150, 750, 1500, 3000) and P. penetrans infected females (F1, F3) densities (F0=control and AC=absolute control without nematode or P. penetrans inoculum) on the build-up of Pasteuria population was investigated over four crop cycles. Two major points of interest were highlighted. First, that within a confined soil volume, densities of P. penetrans can increase >100 times within 2 or 3 crop cycles. Second, from a relatively small amount of spore inoculum, infection of the host is very high. There were more infected females in the higher P. penetrans doses. The root growth data confirms the greater number of females in the controls particularly at the higher inoculum densities in the third and fourth crops. P. penetrans generally caused the fresh root weights to be higher than those in the control. P. penetrans has shown greater reduction of egg masses per plant at most densities. The effects of different initial densities of M. javanica and P. penetrans on the development of the pest and parasite populations were monitored. And no attempt was made to return the P. penetrans spores to the pots after each crop so the build-up in actual numbers of infected females and spores under natural conditions may be underestimated.
Animals ; Bacillus ; pathogenicity ; physiology ; Lycopersicon esculentum ; growth & development ; parasitology ; Pest Control, Biological ; methods ; Tylenchoidea ; growth & development ; microbiology ; Women

The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus of Glomus intraradices and/or infection with pathogenic strain of Phytophthora capsici, and the biological control effect of G. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated with G. intraradices (Gi), (2) plants pre-inoculated with G. intraradices and then infected with P. capsici (Gi+Pc), (3) plants infected with P. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal colonization rate was reduced by about 10% in pathogen challenged plants. Root mortality caused by infection of P. capsici was completely eliminated by pre-inoculation with antagonistic G. intraradices. On the ninth day after pathogen infection, Peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only 21.2% in Gi+Pc-treated roots, compared with the control, respectively. Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase (PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9% in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major POD isozymes (45,000, 53,000 and 114,000) were present in Pc-treated roots, while two major bands (53,000 and 114,000) and one minor band (45,000) were present in spectra of Gi+Pc-treated roots, the 45,000 POD isozyme was significantly suppressed by G. intraradices, suggesting that the 45,000 POD isozyme was induced by the pathogen infection but not induced by the antagonistic G. intraradices. A 60,000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the inoculation of antagonistic G. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper plants infected by P. capsici. The results suggested that G. intraradices is a potentially effective protection agent against P. capsici.
Capsicum ; cytology ; enzymology ; microbiology ; Lignin ; metabolism ; Pest Control, Biological ; methods ; Phyllachorales ; cytology ; physiology ; Phytophthora ; cytology ; physiology ; Plant Proteins ; metabolism

In recent years, root rot diseases of Chinese herbal medicine have been posing grave threat to the development of the traditional Chinese medicine industry. This article presents a review on the occurring situation of the root rot disease, including the occurrence of the disease, the diversity of the pathogens, the regional difference in dominant pathogens,and the complexity of symptoms and a survey of the progress in bio-control of the disease using antagonistic microorganisms. The paper also discusses the existing problems and future prospects in the research.
Animals ; Antibiosis ; Bacteria ; growth & development ; Fungi ; physiology ; Nematoda ; growth & development ; Pest Control, Biological ; methods ; Plant Diseases ; microbiology ; parasitology ; prevention & control ; Plant Roots ; microbiology ; parasitology ; Plants, Medicinal ; microbiology ; parasitology

Four rhizobacteria selected out of over 500 isolates from rhizosphere of the vegetables in China were further studied for suppression of the root-knot nematode and soil-borne fungal pathogens in laboratory and greenhouse in Belgium. They were identified as Brevibacillus brevis or Bacillus subtilis by Biolog test and partial 16s rDNA sequence comparison. They not only inhibited the radial growth of the root-infecting fungi Rhizoctonia solani SX-6, Pythium aphanidermatum ZJP-1 and Fusarium oxysporum f.sp. cucumerinum ZJF-2 in vitro, but also exhibited strong nematicidal activity by killing the second stage larvae of Meloidogyne javanica to varying degrees in the greenhouse. The toxic principles of bacterium B7 that showed the highest juvenile mortality were partially characterized. The active factors were heat stability and resistance to extreme pH values. B7 used either as seed dressing or soil drench significantly reduced the nematode populations in the rhizosphere and enhanced the growth of mungbean plants over the controls in the presence or absence of R. solani.
Alphaproteobacteria ; physiology ; Animals ; Pest Control, Biological ; methods ; Phaseolus ; microbiology ; parasitology ; Seeds ; microbiology ; parasitology ; Survival Analysis ; Survival Rate ; Symbiosis ; physiology ; Tylenchoidea ; microbiology ; pathogenicity

The entomopathogenic bacterium, Xenorhabdus nematophila was isolated from the hemolymph of Galleria mellonella infected with Steinernema carpocapsae. The bacterial cells and its metabolic secretions have been found lethal to the Galleria larvae. Toxic secretion in broth caused 95% mortality within 4 d of application whereas the bacterial cells caused 93% mortality after 6 d. When filter and sand substrates were compared, the later one was observed as appropriate. Similarly, bacterial cells and secretion in broth were more effective at 14% moisture and 25 degrees C temperature treatments. Maximum insect mortality (100%) was observed when bacterial concentration of 4x10(6) cells/ml was used. Similarly, maximum bacterial cells in broth (95%) were penetrated into the insect body within 2 h of their application. However, when stored bacterial toxic secretion was applied to the insects its efficacy declined. On the other hand, when the same toxic secretion was dried and then dissolved either in broth or water was proved to be effective. The present study showed that the bacterium, X. nematophila or its toxic secretion can be used as an important component of integrated pest management against Galleria.
Animals ; Bacterial Proteins ; pharmacology ; Bacterial Toxins ; pharmacology ; Larva ; drug effects ; microbiology ; Moths ; drug effects ; microbiology ; Nematoda ; microbiology ; Pest Control, Biological ; methods ; Survival Analysis ; Survival Rate ; Xenorhabdus ; metabolism ; pathogenicity