1.An experimental study of the effect of methylchlorotetracycline on growth of coronal suture in rats.
Korean Journal of Orthodontics 1980;10(1):7-14
To study some informations on the morphogenesis and developmental process of the coronal suture in rats, the author performed daily oral administrations of semethylchlorotetracycline, a kind of tetracycline group, in the amount of 30mg/kg of body weight to the female rats from the 7th day of pregnaney to the time of delivery. Microscopic evaluation was undertaked on the fetal rats in the experimental group. The subject of this experiment were defined to the fetal rats of each group at 1st, 3rd, 7th and 14th day after birth. All these fetal rats were sacrificed and the heads were removed. All the tissue sections were fixed with 10% formalin, Bouin' and Carnoy' solution and then stained by Hematoxylin-Van Gieson stain, or Feulgen and Rossenbeck, Periodic acid Schiff, and prepared for alcian blue reaction. The results were as follows; 1. The directions of osteogenic fibers were arranged irregulary during first 3 days, but after the 7th day they tended to change radial dirdctions like control group. 2. The density of deep stained cells by Feulgen-Rossenbeck rection were shown less in the experimental group than that in the control group in first 3 days, but there was shown no significant difference between both groups after the 7th day. 3. PAS reaction in early stage was generally negative in the experimental group unlike as in the control group, but diffuse reaction was observed in the loose middle zone like as in the control geoup agter 14th day. 4. Alcian blue reaction was negative in cambial zone, and slightly positive in uniting zone compared with control group in early stage. After 14th day, however, there was observed a tendency of moderately positive reaction.
Administration, Oral
;
Alcian Blue
;
Animals
;
Body Weight
;
Female
;
Formaldehyde
;
Head
;
Humans
;
Morphogenesis
;
Parturition
;
Periodic Acid
;
Periodic Acid-Schiff Reaction
;
Rats*
;
Sutures*
;
Tetracycline
2.Human Bruch's Membrane: Regional and Age-Related Alterations.
Warne HUH ; David A NEWSOME ; Willam R GREEN
Journal of the Korean Ophthalmological Society 1986;27(3):327-331
Changes in the thickness and composition of Bruch's membrane with aging have been described by several observers and related to the development of macular with advancing ageby region, comparing the macular area with more peripheral areas. In the younger (less than. 50 years) eyes we studied, the thickness of macular Bruch's membrane was about equal to that of peripheral Bruch's membrane. With aging, however, the thickness of Bruch's membrane was greater in the periphery than the macula. With increasing age, Bruch's membrane increased in basophilic reaction, PAS positivity and the detectable sialomucin fraction. In the macular region, but not in more peripheral regions, there is a preferential thickening of the interchoriocapillaris "teeth" of Bruch's degeneration by some. Using a combination of histochemical and electronmicroscopic techniques, we have examined the state of Bruch's membrane membrane material. By electronmicroscopy, in older eyes, the elastin layer of Bruch's membrane appears fragmented, while, in the periphery, it is continuous and resembles that of younger eyes. In summary, Bruch's membrane exhibits little regional difference in thickness in younger eyes, but the macular region becomes relatively thinner than more peripheral regions in older eyes. With advancing age, the deposition of extracellular matrix materials in appatite and fragmentation of the elastin layer is dramatically more prominent in the macular region than in the periphery. This regional variation may be related, at least in part, to some aspects of macular degeneration.
Aging
;
Basophils
;
Bruch Membrane*
;
Elastin
;
Extracellular Matrix
;
Humans*
;
Macular Degeneration
;
Membranes
;
Periodic Acid-Schiff Reaction
;
Sialomucins
3.Lipofuscin pigment in adult Clonorchis sinensis.
Seung Yull CHO ; Kye Yong SONG ; Bong Jin RAH
The Korean Journal of Parasitology 1983;21(1):32-39
Adult Clonorchis sinensis from infected human cases may have black pigment in their parenchyme. In present paper, the nature of the pigment was investigated. The pigment distributed in cytoplasm of parenchymal cells of the worms. Histochemically the pigment was positive by periodic acid-Schiff, Ziehl-Neelsen, and lipofuscin stainings. The pigment particle contained many, various sized membrane structure as well as electron dense granules when observed by electron microscopy. Staining characteristics of the black pigment, together with electron microscopic findings, strongly indicated that the pigment was lipofuscin.
parasitology-helminth-trematoda
;
Clonorchis sinensis
;
lipofuscin
;
electron microscopy
;
periodic acid-Schiff
;
Ziehl-Neelsen
4.Comparison of different methods for PAS staining of renal biopsy tissue sections.
Zhan-Mei ZHOU ; Fang YANG ; Wei CAO
Journal of Southern Medical University 2012;32(3):371-373
OBJECTIVETo compare the performance of a modified PAS staining, traditional PAS staining, Lyon's PAS staining, and Tsunahico Watanabe staining for staining sections of renal biopsy tissue.
METHODSThe sections of the renal biopsy tissue were stained with the 4 methods and their staining performance was compared.
RESULTSThe modified PAS staining method produced a better contrast and a higher resolution and showed a greater stability after repeated use than the other 3 methods for staining the renal tissue sections (P<0.05).
CONCLUSIONThe modified PAS staining method shows a better applicability than the other 3 PAS methods for staining sections of renal biopsy tissue.
Biopsy ; Humans ; Kidney ; pathology ; Periodic Acid-Schiff Reaction ; methods ; Staining and Labeling ; methods
5.Three Cases of Hidroacanthoma Simplex.
Dong Ju SHIN ; Jae Won JANG ; Seok Jong LEE ; Jae Bok JUN ; Han Ik BAE
Korean Journal of Dermatology 1999;37(12):1817-1822
Hidroacanthoma simplex (HS) is a rare benign intraepidermal tumor thought to originate from the intraepidermal eccrine duct. Many authors regard it as an intraepidermal form of eccrine poroma but there is still a discussion as to the histogenesis of HS. Clinically, it is more common in older adults and affects both sexes equally. It is found more often on the lower extremities and trunk. The clinical appearance is not characteristic, but the lesions are usually flat or slightly elevated verrucous brown plaques. Histopathologically, the tumor shows characteristic intraepidermal nests and is one of a number of cutaneous lesions showing Borst-Jadasshon phenomenon, that is, tendency to form nests within the epidermis. The tumor cells composing nests show a uniform cuboidal appearance and a round, deeply basophilic nucleus typically. The cytoplasm of tumor cell gives a positive PAS reaction that is diastase-labile. We report herein three cases of HS with the results of immunohistochemical studies.
Adult
;
Basophils
;
Cytoplasm
;
Epidermis
;
Humans
;
Lower Extremity
;
Periodic Acid-Schiff Reaction
;
Poroma
6.A Case of Lichen Amyloidosis Associated with Pdoriasis.
Jai Il YOON ; Seok Beom PARK ; Sang Duck KIM
Korean Journal of Dermatology 1999;37(7):922-925
Psoriasis is merely recognized as a disorder associated with pruritus. But in some cases, psoriasis does induce pruritus which could alter the daily lives of the people affected. Pruritus in psoriasis has been reported to be influenced by various external and internal factors and as a consequence, aggravation of psoriasis is possible by scratching due to the well-known Koebners phenomenen. Lichen amyloidosis, on the other hand, appears as multiple papules with intense pruritus. Recently, there are some evidence emphasizing pruritus as a main factor in forming amyloid deposits. We report a case of 65-year-old lady who was suffering psoriasis for 25 years with associated pruritic papular lesions on both of her thighs, knees and ankles. The diagnosis of Lichen amyloidosis was confirmed by Periodic Acid Schiff and Dylon stain. We discuss the role of pruritus in lichen amyloidosis and psoriasis and speculate that pruritus induced by lichen amyloidosis may induce a vicious cycle in aggravating psoriasis.
Aged
;
Amyloidosis*
;
Ankle
;
Diagnosis
;
Hand
;
Humans
;
Knee
;
Lichens*
;
Periodic Acid
;
Plaque, Amyloid
;
Pruritus
;
Psoriasis
;
Thigh
7.Emperipolesis within Megakaryocyte in Hepatic Hemopoiesis.
Dae Jin KIM ; Hyun Cheol YANG ; Joong Keun AHN ; Sung Su KIM ; Kyung Yong KIM ; Won Bok LEE
Korean Journal of Anatomy 1999;32(5):593-604
This study is designed to detect the emperipolesis in megakaryocyte in fetal liver, which is an important organ of hemopoiesis, during rat development, and to compare the activity of erythropoiesis in fetal liver with that of emperipolesis in megakaryocyte. In order to find that which causes are more related to emperipolesis, we applied periodic acid Schiff reagent, which is special staining method for megakaryocyte and used electron microscope. The size and maturity of magakaryocyte gradually developed with age. The number of megakaryocyte increased in similar proportion to the activity of erythropoiesis. Emperipolesis occurred in more mature megakaryocyte (most stage III). The majority of cells enclosed within megakaryocyte, were the precursor of erythrocytes. Emperipolesis was observed for the first time at 14 day of gestation. The highest frequency of emperipolesis showed 20% of whole megakaryocyte at 16 day of gestation, when the activity of erythropoiesis was most vigorous. The frequency of emperipolesis began to decrease after then, but megakaryocyte was most numerous at 17 day of gestation during fetal and neonatal period. At 19 day of gestation, stage IV megakaryocytes, just before the stage producing platelet, began to appear. Megakaryocyte was not observed after postnatal 10 day. In conclusion, it was found that the emperipolesis in megakaryocyte occurred in the rat fetal liver and was extreme the emperipolesis most observed at the time of most vigorous erythropoiesis during the development of rat fetal liver. It is suggested that the frequency of emperipolesis within megakaryocyte is more closely related with the activity of erythropoiesis in fetal liver than the that of megakaryocytopoiesis, before bone marrow acts as an important organ of hematopoiesis. It is also suggested that the emperipolesis contributes to the production of platelet during gestation period and the maturation of erythrocyte.
Animals
;
Blood Platelets
;
Bone Marrow
;
Emperipolesis*
;
Erythrocytes
;
Erythropoiesis
;
Hematopoiesis
;
Liver
;
Megakaryocytes*
;
Periodic Acid
;
Pregnancy
;
Rats
;
Thrombopoiesis
8.The value of Alcian blue periodic acid Schiff staining and Ki-67 expression in diagnosing gastric reactive epithelial hyperplasia and dysplasia.
Zhong Yue SHI ; Wei Hua HOU ; Ying WANG ; Zhong Qiu TIAN ; Qing CAO ; Xin Meng GUO ; Jun LU ; Xue LI ; Hong CHEN ; Mu Lan JIN
Chinese Journal of Pathology 2022;51(8):713-718
Objective: To investigate the clinicopathological characteristics of reactive epithelial hyperplasia and dysplasia in the stomach, as well as the clinical value of mucin special staining and proliferating cell nuclear antigen (Ki-67) in distinguishing the two gastric lesions. Methods: The clinical pathological data of 63 patients with gastric reactive epithelial hyperplasia, 54 patients with low-grade dysplasia, and 63 patients with high-grade dysplasia diagnosed from May 2018 to May 2021 in Beijing Chaoyang Hospital, Capital Medical University, Beijing, China were analyzed. Alcian blue periodic acid Schiff (AB-PAS) and Ki-67 staining were performed to examine the mucin staining pattern, number of Ki-67 positive cells, Ki-67 staining patterns in the three groups of lesions, and histopathologic characteristics. Results: The positive rates of AB-PAS in the reactive epithelial hyperplasia and gastric dysplasia groups were 87.3%(55/63) and 10.3%(12/117), respectively. The expression of AB-PAS in the reactive epithelial hyperplasia was gradually increased from the base to the surface of the epithelium. In low-grade dysplasia and high-grade dysplasia, there was no mucin present in the dysplasia epithelium. The difference between the two groups was statistically significant (P<0.01). The positive rate of Ki-67 in the epithelial reactive hyperplasia (>10%) was 81.0% (51/63), and the positive cells were mainly located in the neck and middle parts of the mucosal glands (58/63, 92.1%). In the low-grade dysplasia group, the positive rate of Ki-67 (>10%) was 90.7%(49/54); the positive cells were mainly located in the upper mucosa (33/54, 61.1%), showing a banded distribution pattern; in the high-grade dysplasia group, the positive rate (>10%) was 95.2%(60/63), and the positive cells were mainly located in the whole mucosa (49/63, 77.8%), showing a diffuse/diffuse scattered distribution pattern. The three groups had statistically different rates and distribution patterns of Ki-67 expression (P<0.01). Conclusion: The gastric epithelial reactive hyperplasia and dysplasia can be differentiated using clinicopathological features, AB-PAS staining and Ki-67 expression pattern.
Alcian Blue
;
Humans
;
Hyperplasia
;
Ki-67 Antigen/metabolism*
;
Periodic Acid
;
Staining and Labeling
;
Stomach Neoplasms/diagnosis*
9.Correlation between the expression of collgen IV, VEGF and vasculogenic mimicry.
Xi-shan HAO ; Bao-cun SUN ; Shi-wu ZHANG ; Xiu-lan ZHAO
Chinese Journal of Oncology 2003;25(6):524-526
OBJECTIVETo study the relationship between collgen (Col) IV, VEGF secreted by the tumor cells and vasculogenic mimicry (VM).
METHODS158 bi-phase differential malignant tumor specimens were alloted and made into tissue microarray. These tissue microarray sections were stained with CD31, periodic acid-Schiff (PAS) and Col IV. Subsequently, distributive trait of Col IV and the difference of VEGF expression were analyzed.
RESULTSThe basement membrane of VM was PAS and Col IV positive. The expression of VEGF in bi-phase differential malignant tumor with VM was less than that in those without VM. The difference of VEGF expression in malignant melanoma and alveolar rhabdomyosarcoma was significant (P < 0.05).
CONCLUSIONCollgen IV and periodic acid-Schiff positive material take part in constructing the basement membrane of vasculogenic mimicry. The difference of the VEGF expression proves that vasculogenic mimicry can sustain the tumor blood supply.
Collagen Type IV ; analysis ; Humans ; Immunohistochemistry ; Neoplasms ; blood supply ; chemistry ; pathology ; Periodic Acid-Schiff Reaction ; Vascular Endothelial Growth Factor A ; analysis
10.A Clinicopathological Study of the Multiple Trichoepitheliomas.
Tae Ahn CHUNG ; Kyung Hyung SEO ; Ho Sun JANG ; Kyung Sool KWON ; Chang Keun OH
Korean Journal of Dermatology 1997;35(5):909-914
BACKGROUND: The diagnostic dilemma in differentiation between multiple and solitary trichoepitheliomas from basal cell carcinoma(BCC) is well documented. Some authors reported that up to 35% of trichoepitheliomas were misdiagnosed initially as BCC, especially the keratinizing variant. OBJECTIVE: The purpose of this study was to review the clinicopathological characteristics of the multiple trichoepitheliomas and to suggest specific findings and useful special staining methods for differentiating from BCC. METHODS: We reviewed 14 cases of multiple trichoepitheliomas from a clinicopathological aspect. We performed H&E, periodic acid Schiff, Giemsa, Fite, and alcian blue staining using the formalin-fixed paraffin-embedded samples of multiple trichoepitheliomas. RESULTS: The average age at onset was 24.7 and 78.6% of the cases showed autosomal dominant inheritance patterns. The sites of frequent involvement were the nasolabial folds and nose, and the average size of the lesions was 4.3 x 4.3 mm. The most important histopathological finding in differentiation was papillary mesenchymal tissue, and this was well observed with the Giemsa stain. Several other findings were also useful in differentiation, such as stellate structures in cystic walls, parakeratosis and eosinophilic necrotic bodies in the cystic walls, primitive hair follicles, epidermal connections, and mast cell infiltration. CONCLUSION: Several staining methods, such as the Giemsa, periodic acid Schiff, Fite, and alcian blue stains, were useful in differentiating the trichoepitheliomas from BCCs.
Alcian Blue
;
Azure Stains
;
Coloring Agents
;
Eosinophils
;
Hair Follicle
;
Inheritance Patterns
;
Mast Cells
;
Nasolabial Fold
;
Nose
;
Parakeratosis
;
Periodic Acid