3.Postoperative Changes of Continuous Curvilinear Capsulorhexis.
Journal of the Korean Ophthalmological Society 1998;39(6):1153-1158
As a growing number of surgeons are perforining cataract extraction by phacoernulsification and the continuous curvilinear capsulorhexis [CCC] has rapidly increased in popularity as the anterior capsulotomy technique, its advantages have been reported recently. But its complications induced by capsular contraction after ccc have been noted, and so we have studied the change in anterior capsular opening area and refractive error after ccc. One surgeon performed cataract extraction using ccc and phacoernulsification, and implanted posterior chamber IOL [optic size 5. 5mm, Pharmacia 812A] in the bag in 48 eyes, and we measured the area of anterior capsular opening and refractive error [spherical equivalent] at I week and 3 months after operation. The average percentage of anterior capsular shrinkage rate at 3 months was 14.8% and showed statistically significant difference [P<0.05], but. had not correlation with initial ccc size. The average refractive change at 3 months was 0. 2D hyperopic shift but did not show statistically significant difference.
Capsulorhexis*
;
Cataract Extraction
;
Perforin
;
Phacoemulsification
;
Refractive Errors
4.The Role of the Cytokines (IL-12, IFN-gamma) in the Generalized Shwartzman Reaction Induced by Vibrio Vulnificus Cytolysin.
Seok Don PARK ; Dong Lim KIM ; Sang Won LEE ; Jae Hoon CHUN ; Seung Hoon CHA
Korean Journal of Dermatology 2000;38(5):622-628
No Abstract Available.
Cytokines*
;
Perforin*
;
Shwartzman Phenomenon*
;
Vibrio vulnificus*
;
Vibrio*
5.Induction of Apoptosis by Vibrio vulnificus Cytolysin Through Activation of Caspase-3 in Endothelial Cells.
Byung Hyun PARK ; Kang Beom KWON ; Young Hoon LEE ; Jae Han JANG ; Jin Woo PARK
Journal of Bacteriology and Virology 2001;31(4):333-341
No abstract available.
Apoptosis*
;
Caspase 3*
;
Endothelial Cells*
;
Perforin*
;
Vibrio vulnificus*
;
Vibrio*
6.A case report of adult onset of primary hemophagocytic syndrome with literature review.
Yi-ni WANG ; Zhao WANG ; Xiao-lin WANG
Chinese Journal of Hematology 2012;33(4):291-293
OBJECTIVETo introduce the clinical manifestations and laboratory tests of adult onset of primary hemophagocytic syndrome (HPS), and to investigate the essentials of diagnosis and the genotype characteristics in adult onset patient.
METHODSThe definite diagnosis of HPS was made according to HLH-2004. Exons of PRF1, STX11, UNC13D, SH2D1A and RAB27A genes coding region were amplified using polymerase chain reaction.
RESULTSA 48-year-old man was admitted to our hospital because of recurrent fever, pancytopenia and lymph node enlargement. His laboratory test revealed bone marrow hemophagocytosis, elevated ferritin level (2000 µg/L), reduced level of NK cell activity (20.13%) and elevated soluble CD25 level (12277 U/ml). Based on the HLH-2004 diagnostic criteria, the patient was diagnosed as HPS. The patient had viral infection, and no other primary disease was identified that would cause HPS. The patient responded poorly to anti-viral therapy. DNA sequencing was used to confirm that perforin gene mutations might be one of the causes of the patient suffered from primary HPS.
CONCLUSIONSAlthough primary HPS usually affects infants and young children, it also occurred in teens and adults. It is essential to perform genetic screenings to patient whose illnesses recur with unknown causes. In addition, detection of molecular genetic alterations can be used to distinguish primary HPS from acquired HPS.
Humans ; Lymphohistiocytosis, Hemophagocytic ; diagnosis ; genetics ; Male ; Middle Aged ; Perforin ; genetics
7.The Expression of Peforin, Fas-ligand, and Granzyme B in Peripheral Blood Lymphocytes of Renal Allograft Recipients.
Gyu Tae SHIN ; Seung Jung KIM ; Kyung Ai MA ; Young Il CHOI ; Jung Eun KIM ; Jong Woo LEE ; Heung Soo KIM ; Tae Seung LEE ; Chang Kwon OH ; Do Hun KIM
Korean Journal of Nephrology 2002;21(3):414-422
BACKGROUND: Previous findings demonstrated that the expression of cytotoxic effector molecules is increased in acute rejection of renal allografts. In the present study, we serially examined the gene expression of perforin, granzyme B and Fas ligand(FasL) in peripheral blood lymphocytes(PBLs) of renal allograft recipients to assess the potential of their expression as a marker of acute rejection. METHODS: PBLs were isolated from blood samples taken on days 2, 4, 6, 8, 10 and 12 after transplantation. Competitive PCR was performed to evaluate the abundance of mRNA of perforin, granzyme B and FasL. The mean value of each molecule plus 2 SD for the control group was set as a discriminatory level. RESULTS: When all measured samples were compared, perforin expression was significantly higher in patients with acute rejection than in the control group(1.84+/-3.01 versus 0.71+/-0.48, p=0.01). The percentage of perforin expression exceeding the discriminatory level was also significantly higher in patients with acute rejection(p=0.0003). Five patients in the rejection group(5/7, 71.4%) showed perforin expression exceeding the discriminatory level, while only 1 patient in the control group did so(1/8, 12.5%)(p= 0.02). Perforin expression of days 0 and 1 of rejection crisis was the highest over the study period. No consistent pattern of granzyme B and FasL expression was identified in relation to rejection crisis. CONCLUSION: Gene expression of perforin by PBLs was upregulated in accordance with acute rejection, thus offering the possibility that it may be utilized as a marker of acute rejection.
Allografts*
;
Gene Expression
;
Granzymes*
;
Humans
;
Kidney Transplantation
;
Lymphocytes*
;
Perforin
;
Polymerase Chain Reaction
;
RNA, Messenger
8.Development of NK cell expansion methods using feeder cells from human myelogenous leukemia cell line.
Blood Research 2014;49(3):154-161
BACKGROUND: Natural killer (NK) cells constantly survey surrounding tissues and remove newly generated cancer cells, independent of cancer antigen recognition. Although there have been a number of attempts to apply NK cells for cancer therapy, clinical application has been somewhat limited because of the difficulty in preparing a sufficient number of NK cells. Therefore, ex vivo NK cell expansion is one of the important steps for developing NK cell therapeutics. METHODS: CD3+ depleted lymphocytes were cocultured with IL-2 and with feeder cells (peripheral blood mononuclear cells [PBMCs], K562, and Jurkat) for 15 days. Expanded NK cells were tested for cytotoxicity against cancer cell lines. RESULTS: We compared feeder activities of three different cells-PBMC, K562, and Jurkat. K562 expanded NK cells by almost 20 fold and also showed powerful cytotoxic activity against cancer cells. K562-NK cells remarkably expressed the NK cell activation receptors, NKG2D, and DNAM-1. K562-NK cells exhibited more than two-fold production of cytotoxic granules compared with Jurkat-NK cells, producing more perforin and granzyme B than naive NK cells. CONCLUSION: Our findings suggest that K562 are more efficient feeder cells than Jurkat or PBMCs. K562 feeder cells expanded NK cells by almost 20 fold and showed powerful cytotoxic activity against cancer cells. We herein propose an intriguing approach for a design of NK cell expansion.
Cell Line*
;
Feeder Cells*
;
Granzymes
;
Humans
;
Interleukin-2
;
Killer Cells, Natural*
;
Leukemia, Myeloid*
;
Lymphocytes
;
Perforin
9.Expression of perforin and granzyme-B in peripheral blood lymphocyte in patients with prostate cancer and the clinical significance.
Jingliang HE ; Liuxun LI ; Wensu WEI ; Jingchao WEI ; Zhi LONG ; Yichuan ZHANG ; Leye HE
Journal of Central South University(Medical Sciences) 2015;40(4):387-391
OBJECTIVE:
To explore the expression of perforin and granzyme-B in peripheral blood lymphocyte (PBL) in patients with prostate cancer (PCa) and the clinical significance.
METHODS:
The expressions of perforin and granzyme-B in PBL were detected by fluorescence quantitative reverse transcription polymerase chain reaction. The results of perforin and granzyme-B expression were compared among patients with PCa (n=60), patients with BPH (benign prostatic hyperplasia, n=40) and healthy controls (n=20).
RESULTS:
Th e expressions of perforin and granzyme-B in patients with PCa were significantly lower than that in patients with BPH or that in the healthy controls (P<0.05), respectively. Furthermore, in PCa patients with low pathological grade, the expressions of perforin and granzyme-B in PBL was statistically higher than that in patients with high pathological grade (P<0.05). The expressions of perforin and granzyme-B in PCa patients at high clinical stage was statistically lower than that in PCa patients at low clinical stage (P<0.05).
CONCLUSION
The results of this study suggest that development and progression of PCa might be associated with poor immune status of patients.
Case-Control Studies
;
Granzymes
;
metabolism
;
Humans
;
Lymphocytes
;
enzymology
;
Male
;
Perforin
;
metabolism
;
Prostatic Hyperplasia
;
Prostatic Neoplasms
;
immunology
10.Analysis of PRF1gene variant in a child with late-onset familial hemophagocytic lymphohistiocytosis type 2 and severe central nervous system disease.
Qiulin DING ; Xia GUO ; Qiang LI
Chinese Journal of Medical Genetics 2019;36(6):592-594
OBJECTIVE:
To detect genetic mutations in a child with late-onset hemophagocytic lymphohistiocytosis (HLH).
METHODS:
Clinical data of an 8-year-5-month-old girl with recurrent HLH and severe central nervous system disease was analyzed. Next-generation sequencing was used to detect mutation in the exons and adjacent introns of 17 genes associated with HLH. Suspected mutations were confirmed by Sanger sequencing. Influence of mutations on protein function was predicted with SIFT and PolyPhen-2 software.
RESULTS:
The child was found to carry compound heterozygous mutations of the PRF1 gene. Among these, the c.1349C>T (p.Thr450Met) mutation, with a SIFT predictive value of -4.921 (Deleterious variant) and a PolyPhen-2 predictive value of 1.000 (Probably damaging), was inherited from her father and known to be pathogenic. The c.1273dupT (p.Trp425fsX457) mutation was inherited from her mother and previously unreported, which resulted in the deletion of almost the entire C2 domain (amino acid residues 413 to 540) and carboxyl terminal of perforin, which seriously affected the function of the protein.
CONCLUSION
The c.1349C>T (p.Thr450Met) and c.1273 dupT (p.Trp425fsX457) compound heterozygous mutations of the PRF1 gene probably underlie the disease in this patient.
Central Nervous System Diseases
;
Child
;
Exons
;
Female
;
Humans
;
Lymphohistiocytosis, Hemophagocytic
;
Mutation
;
Perforin