1.Studies on chemical constituents of the mycelia from fermented culture of Flammulina velutipes.
China Journal of Chinese Materia Medica 2003;28(11):1038-1040
OBJECTIVETo study the chemical constituents from the mycelia of Flammulina velutipes.
METHODThe compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of spectral analysis (IR, EL-MS, FAB-MS, 1H-NMR, 13NMR).
RESULTSeven compounds were identified as cyclo-(R-pro-R-leu) (1), cyclo-(R-isoleu-R-leu) (2), phenylalanine (3), alanine (4), leucine (5), guanosine (6), adenosine (7),
CONCLUSIONThe compounds 1-6 were isolated from the mycelia of Flammulina velutipes for the first time.
Agaricales ; chemistry ; Alanine ; chemistry ; isolation & purification ; Dipeptides ; chemistry ; isolation & purification ; Fermentation ; Leucine ; chemistry ; isolation & purification ; Mycelium ; chemistry ; Peptides, Cyclic ; chemistry ; isolation & purification ; Phenylalanine ; chemistry ; isolation & purification
2.Tyrosinase inhibition of Potentilla bifurca.
Xianglan PIAO ; Yanze TIAN ; Xiaoyuan MI ; Jian CUI
China Journal of Chinese Materia Medica 2009;34(15):1952-1954
OBJECTIVETo identify the tyrosinase inhibitory constituent quickly from Potentilla bifurca.
METHODThe active constituent was found through fraction collecting and tyrosinase inhibitory activity by bioassay-linked HPLC method.
RESULTThe methanol extracts and BuOH fraction of Potentilla bifurca showed strong tyrosinase inhibitory activities. From BuOH fraction of Potentilla bifurca, the tyrosinase inhibitory constituent was isolated and identified as flavonoid, quercetin-4'-O-beta-D-glucoside. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid (IC50 = 0.28 mmol x L(-1)) with IC50 value of 0.001 9 mmol x L(-1).
CONCLUSIONBioassay-linked HPLC fractionation method was provided for determination the active constituents quickly from herbal medicines.
Enzyme Inhibitors ; chemistry ; isolation & purification ; Kinetics ; Peptides ; chemistry ; isolation & purification ; Plant Extracts ; chemistry ; isolation & purification ; Potentilla ; chemistry
3.Three cyclic dipeptides from the root of Psammosilene tunicoides.
Zhong-tao DING ; Zhi-juan BAO ; Xue-qiong YANG ; Ming JIANG
China Journal of Chinese Materia Medica 2003;28(4):337-339
OBJECTIVE[corrected] To study the cyclic peptides from Psammosilene tunicoides.
METHODThe constituents were separated and purified with chromatographic methods, identified by NMR, MS, UV and IR.
RESULTThree cyclic dipeptides: cyclo(-Pro-Val-) (1), cyclo(-Pro-Ala-) (2) and cyclo(-Pro-Pro-) (3), were isolated and identified.
CONCLUSIONCompound 1 and 2 are new natural products, compound 3 was isolated for the first time from Psammosilene tunicoides.
Caryophyllaceae ; chemistry ; Dipeptides ; chemistry ; isolation & purification ; Molecular Structure ; Peptides, Cyclic ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry
4.Cyclopeptides from Rubia schumanniana.
Bin KUANG ; Jun-ting FAN ; Si-meng ZHAO ; Ning-hua TAN
China Journal of Chinese Materia Medica 2012;37(17):2563-2570
OBJECTIVETo separate and identify cyclopeptides of tubers of Rubia schumanniana.
METHODThe 70% methanol extracts from tubers of Rubia schumanniana were separated and purified by silica gel, RP-18, Sephedax LH-20 and HPLC. Their structures were identified by spectral analysis.
RESULTNine cyclopeptides were separated and identified as RA- II (1), RA-V (2), RA-VIII (3), rubiyunnanin C (4), RA-X (5), RY-II (6), RA- I (7), RA-XIII (8) and RA-XIII-OMe (9), respectively.
CONCLUSIONAll of nine cyclopeptides were separated from R. schumanniana for the first time.
Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Molecular Structure ; Peptides, Cyclic ; analysis ; isolation & purification ; Rubia ; chemistry
5.Isolation and mass spectrometric analysis of antioxidant peptides from enzymatic hydrolysates of roasted pills of Asini Corii Colla.
Xiao-Chen LI ; Li-Hua YAN ; Zhi-Min WANG ; Qi-Wei ZHANG ; Jian-Ping GAO ; Liang-Mian CHEN ; Jin-Yu WANG ; Yan TONG ; Gui-Feng ZHANG
China Journal of Chinese Materia Medica 2013;38(7):1021-1025
A fraction named GFC-1 with high antioxidant activities in vitro was isolated from the enzymatic hydrolysates of roasted pills of Asini Corii Colla, and the peptides in this fraction were identified. The enzymatic hydrolysates were isolated and purified with anion exchange chromatography and Sephadex G-25 filtration chromatography successively. GFC-1, a fraction isolated from the hydrolysates, exhibited the highest DPPH and ABTS scavenging capacity (DPPH 47. 95% at 2.0 g x L(-1) and ABTS 97.20% at 0.40 g x L(-1). Nine peptides from GFC-1 were identified by LC-ESI-MS/MS coupled with TurboSEQUEST search software and Swiss-Prot data base, and a high repetition core sequence GPAGPP*GPP* was also found.
Animals
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Antioxidants
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chemistry
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isolation & purification
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Equidae
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Hydrolysis
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Mass Spectrometry
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Peptides
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chemistry
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isolation & purification
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Protein Hydrolysates
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chemistry
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Skin
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chemistry
6.Research development of chemistry and bioactive activity of plant peptides.
Yan LIANG ; Xiao-qin WU ; Ying ZHANG
China Journal of Chinese Materia Medica 2006;31(9):709-714
As the technologies of separation, purification and determination develop rapidly, more and more peptide compounds, which have special bioactive and medical value, have been separated from natural plants, such as oligopeptides and cyclopeptides. The chemical structures and function of these plant peptides have been researched profoundly. This paper mainly reviews the composition, structure, bioactive function and medicine value of representative plant peptides in recent years, and can give some references about research and application of plant bioactive peptides.
Animals
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Antihypertensive Agents
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chemistry
;
isolation & purification
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pharmacology
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Antineoplastic Agents, Phytogenic
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chemistry
;
isolation & purification
;
pharmacology
;
Antioxidants
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chemistry
;
isolation & purification
;
pharmacology
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Glutathione
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chemistry
;
isolation & purification
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pharmacology
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Gramicidin
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chemistry
;
isolation & purification
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pharmacology
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Humans
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Hypoglycemic Agents
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chemistry
;
isolation & purification
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pharmacology
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Oligopeptides
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chemistry
;
isolation & purification
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pharmacology
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Peptides
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chemistry
;
isolation & purification
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pharmacology
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Peptides, Cyclic
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chemistry
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isolation & purification
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pharmacology
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Plants
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chemistry
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Plants, Medicinal
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chemistry
7.The recent research progress of chemistry of marine natural products.
Qing-wen SHI ; Li-geng LI ; Yu-fang WANG ; Chang-hong HUO ; Man-li ZHANG
Acta Pharmaceutica Sinica 2010;45(10):1212-1223
Ocean is a unique and excellent resource that provides a diverse array of intriguing natural products. Marine natural products have demonstrated significant and extremely potent biological activities and have captured the attention of natural products chemists in the past few decades. It is increasingly recognized that a wealth of fascinating natural products and novel chemical entities will play a dominant role in the discovery of useful leads for the development of pharmaceutical agents and provide useful probes to lead to breakthroughs in a variety of life-science fields. This article focused on the research progress of chemistry of marine natural products in recent five years.
Alkaloids
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chemistry
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isolation & purification
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pharmacology
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Animals
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Anti-Infective Agents
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chemistry
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isolation & purification
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pharmacology
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Antineoplastic Agents
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chemistry
;
isolation & purification
;
pharmacology
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Aquatic Organisms
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chemistry
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Biological Products
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chemistry
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isolation & purification
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pharmacology
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Humans
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Macrolides
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chemistry
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isolation & purification
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pharmacology
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Marine Biology
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Marine Toxins
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chemistry
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isolation & purification
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pharmacology
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Molecular Structure
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Peptides
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chemistry
;
isolation & purification
;
pharmacology
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Steroids
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chemistry
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isolation & purification
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pharmacology
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Terpenes
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chemistry
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isolation & purification
;
pharmacology
8.Study on chemical constituents from leaves of Tripterygium wilfordii.
Xu CAO ; Chuangjun LI ; Jingzhi YANG ; Baixing WEI ; Yongming LUO ; Dongming ZHANG
China Journal of Chinese Materia Medica 2011;36(8):1028-1031
In order to study the chemical constituents of the leaves of Tripterygium wilfordii and provide references for the bio-active study, we isolated nine compounds from the dried leaves of Tripterygium wilfordii. Their structures were determined by application of spectroscopic (NMR, MS) and chemical methods. These compounds were isolated and identified as (+)-lyoniresinol (1), (+)-isolariciresinol (2), burselignan (3), dibutyl phthalate (4), cyclo-(S-Pro-R-Phe) (5), cyclo-(S-Pro-R-Leu) (6), cyclo-(S-Pro-S-Ile) (7), 3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (8) and daucosterol (9). Compounds 1-3, 5-8 were isolated from this plant for the first time.
Anisoles
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chemistry
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isolation & purification
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Dibutyl Phthalate
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chemistry
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isolation & purification
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Lignin
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chemistry
;
isolation & purification
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Magnetic Resonance Imaging
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methods
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Mass Spectrometry
;
methods
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Naphthalenes
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chemistry
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isolation & purification
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Naphthols
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chemistry
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isolation & purification
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Peptides, Cyclic
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chemistry
;
isolation & purification
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Plant Extracts
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chemistry
;
isolation & purification
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Plant Leaves
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chemistry
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Sitosterols
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chemistry
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isolation & purification
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Tripterygium
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chemistry
9.Peptides and polyketides isolated from the marine sponge-derived fungus Aspergillus terreus SCSIO 41008.
Xiao-Wei LUO ; Yun LIN ; Yong-Jun LU ; Xue-Feng ZHOU ; Yong-Hong LIU
Chinese Journal of Natural Medicines (English Ed.) 2019;17(2):149-154
Two new isomeric modified tripeptides, aspergillamides C and D (compounds 1 and 2), together with fifteen known compounds (compounds 3-17), were obtained from the marine sponge-derived fungus Aspergillus terreus SCSIO 41008. The structures of the new compounds, including absolute configurations, were determined by extensive analyses of spectroscopic data (NMR, MS, UV, and IR) and comparisons between the calculated and experimental electronic circular dichroism (ECD) spectra. Butyrolactone I (compound 11) exhibited strong inhibitory effects against Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB) with the IC being 5.11 ± 0.53 μmol·L, and acted as a noncompetitive inhibitor based on kinetic analysis.
4-Butyrolactone
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analogs & derivatives
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chemistry
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isolation & purification
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pharmacology
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Animals
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Aspergillus
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chemistry
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Chemistry Techniques, Analytical
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Dipeptides
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chemistry
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isolation & purification
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pharmacology
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Enzyme Inhibitors
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chemistry
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isolation & purification
;
pharmacology
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Indoles
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chemistry
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isolation & purification
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pharmacology
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Molecular Structure
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Mycobacterium tuberculosis
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drug effects
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Peptides
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chemistry
;
isolation & purification
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pharmacology
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Polyketides
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chemistry
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isolation & purification
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pharmacology
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Porifera
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microbiology
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Protein Tyrosine Phosphatases
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chemistry
10.Progress on the studies of cyclic lipopeptides.
Jin-shan TANG ; Hao GAO ; Yi DAI ; Kui HONG ; Xin-sheng YAO
Acta Pharmaceutica Sinica 2008;43(9):873-883
Cyclic lipopeptide, also named as acylpeptide, which was characteristic with novel structures, was paid more attention in the recent years. Cyclic lipopeptide showed various bioactivities including antibacterial, anti-tumor, anti-inflammatory, etc. Cyclic lipopeptide originated mainly from the second metabolites of microorganism, such as Cyanobacterium, Bacterium, Actinomyces, etc. The bacteria included the genus of Bacillus and Pseudomonas. In this account, the review has been made on the development of cyclic lipopeptide.
Anti-Bacterial Agents
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pharmacology
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Antibiotics, Antineoplastic
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pharmacology
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Bacillus
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chemistry
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Cyanobacteria
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chemistry
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Daptomycin
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isolation & purification
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pharmacology
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Humans
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Lipopeptides
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chemistry
;
isolation & purification
;
pharmacology
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Peptides, Cyclic
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chemistry
;
isolation & purification
;
pharmacology
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Pseudomonas
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chemistry