1.Thirteen new peptaibols with antimicrobial activities from Trichoderma sp.
Pan TANG ; Dan HUANG ; Kai-Xuan ZHENG ; Dan HU ; Ping DAI ; Chuan-Hui LI ; Sheng-Ying QIN ; Guo-Dong CHEN ; Xin-Sheng YAO ; Hao GAO
Chinese Journal of Natural Medicines (English Ed.) 2023;21(11):868-880
From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K (1-7), and six new 14-residue peptaibols, harzianins NPDG J-O (8-13). Additionally, four previously characterized 18-residue peptaibols neoatroviridins A-D (14-17) were also identified. The structural configurations of the newly identified peptaibols (1-13) were determined by comprehensive nuclear magnetic resonance (NMR) and high-resolution electrospray ionization tandem mass spectrometry (HR-ESI-MS/MS) data. Their absolute configurations were further determined using Marfey's method. Notably, compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue. In antimicrobial assessments, all 18-residue peptaibols (1-7, 14-17) exhibited moderate inhibitory activities against Staphylococcus aureus 209P, with minimum inhibitory concentration (MIC) values ranging from 8-32 μg·mL-1. Moreover, compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709, with a MIC value of 16 μg·mL-1.
Peptaibols/chemistry*
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Trichoderma/metabolism*
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Tandem Mass Spectrometry/methods*
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Anti-Infective Agents/pharmacology*
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Spectrometry, Mass, Electrospray Ionization/methods*