No abstract available.
Immunoglobulin G* ; Pentetic Acid* ; Polymers*

We evaluated the renal function could be estimated by the visual analysis of the perfusion images of Tc-99m DTPA renal scan. Renal scan, creatinine clearance(CCr) and serum creatinine(s-Cr) were obtained in 105 patients. Intensity of renal activity(RA) at 6 sec perfusion image after the first visualization of abdominal aortic activity(A or A) was considered as the parameter for estimating renal function. Intensity of RA was scored from grade(Gr.) 1 to 3(Gr. 1: RAsplenic activity(SA), Gr. 2: RA A or A, Gr. 3: RA60 mL/min. The P.P.V. of Gr. 2 RA was 92% for s-Cr from 1.5 to less than 3.5mg/dL and 83% for CCr value from 20 to less than 60mL/min. The P.P.V. of Gr. 3 RA was 95% for s-Cr3.5mg/dL and 95% for CCr<20mL/min. As the intensity of RA of perfusion images decreased, renal function more decreased. We could detect renal dysfunction by visual analysis of perfusion images of renal scan.
Creatinine ; Humans ; Pentetic Acid* ; Perfusion*

The previous reports have indicated that both the DMSA scan and the DTPA scan were significantly related with the relative renal function ( RRF) and there was a close relationship between the DMSA scan and the DTPA scan in the evaluation of the RRF We reviewed our experience with 24 cases, in which the DMSA scan and the DTPA scan were performed within 2 weeks from January 1987 to April 1991. The DMSA scan was more reliable than the DTPA scan and the difference of the RRF between right and left side by the DMSA scan was more apparent than that by the DTPA scan. However, in infants, both scans showed the reversed results. Our study suggests that DMSA scan is more reliable than the DTPA scan in the evaluation of the RRF, but both renal scans are inappropriate in infants, especially under the age of 1 months.
Humans ; Hydronephrosis* ; Infant ; Pentetic Acid* ; Succimer*

PURPOSE: We evaluated whether increased split renal function ratio (%) on the renal scan with Tc-99m diethylenetriamine pinta-acetic acid (DTPA) represent elevated renal function in the obstructed kidney. MATERIALS AND METHODS: From 1994 to 1995, we evaluated 36 patients with unilateral hydronephrosis due to ureteropelvic junction (UPJ) obstruction and split renal function ratios of the affected kidneys were calculated using Tc-99m DTPA renal scan. RESULTS: Of the 36 patients, 7 patients showed that split renal function ratios of the affected kidneys were all over 52%. To assess the significance of the split renal function ratio on the Tc-99m DTPA renal scan, we performed Tc-99m mercaptoacetyltriglycine (MAG3) renal scan with the time interval of 3 days and calculated the split renal function ratios of the affected kidneys on the MAG3 renal scan in all 7 patients. The split renal function ratios of the affected kidneys on the MAG3 renal scan were also higher than those of the normal kidney. And the split renal function ratios of the affected kidneys on the MAG3 renal scan were well correlated with those of DTPA renal scan (r=0.83). CONCLUSIONS: Our results suggest that the increased split renal function ratio in affected kidney due to UPJ obstruction is not an artifact of the DTPA renogram.
Artifacts ; Humans ; Hydronephrosis ; Kidney* ; Pentetic Acid

Re-188 is suitable for endovascular liquid-balloon brachytherapy for the prevention of restenosis after angioplasty. Re-188 was concentrated to 3700 MBq/ml and labeled with DTPA. According to dosimetric calculation, it took 420 seconds using Re-188 solution with concentration of 3700 MBq/ml to irradiate 17.6 Gy to the target at 1 mm from the balloon surface. Software was made to estimate the irradiation time. MIRD calculation with dynamic bladder model yielded the whole body dose of Re-188-DTPA as 0.005mGy/MBq in case of balloon rupture and release of the whole amount into the blood.
Angioplasty ; Brachytherapy ; Pentetic Acid ; Rupture ; Urinary Bladder

In clinical practice, Tc-99m DTPA renal scan has been using for screening for the presence of renal dysfunction to determine the need for early treatment in kidney transplantation patients. We measured glomerular filtration rate(GFR) using Gates method during the routine Tc-99m DTPA renal scan, predicted creatinine clearance estimated by Cockcroft and Gault formula and 24-hour creatinine clearance and compared each other in 88 kidney transplantation patients simultaneously. The range of renal uptake(%) of Tc-99m DTPA was from 1.8% to 10.4% and the correlation between 24-hour creatinine clearance and renal uptake showed Y=7.176X8.975Y=creatinine clearance(ml/min), X=renal uptake(%) and the correlation coefficient was 0.771. The correlation coefficient between GFR (ml/min) using Tc-99m DTPA renal scan and predicted creatinine clearance was 0.765. The correlation coefficient between predicted creatinine clearance and 24-hour creatinine clearance was 0.850. We concluded that the measurement of GFR using Tc-99m DTPA renal scan was clinically useful in kidney transplantation patients with the advantage of simplicity, low expense, opportunity for renal imaging.
Creatinine ; Filtration ; Humans ; Kidney Transplantation* ; Kidney* ; Mass Screening ; Pentetic Acid*

Tc-99m mercaptoacetyltriglycine (MAG3) is a new Tc-99m renal agent that is excreted by tubular function. To evaluate the efficacy of Tc-99m MAG3, renal scans with Tc-99m MAG3 and Tc-99m diethylenetriamine penta-acetic acid(DTPA) were performed in patients with upper urinary tract obstruction within the time interval of 3 days. Of the 36 patients 17 had ureteropelvic junction (UPJ) strictures, 5 had renal stones, 10 had ureteral stones, and 4 had ureterovesical junction (UVJ) strictures. The patient ages were ranged from 1 to 72 years and the numbers of male and female were 22 and 14, respectively. Split renal function ratio(%), time parameters and image quality using target to background ratio of the Tc-99m MAG, renal scan were compared with those of Tc-99m DTPA, in terms of age group (less than 16, 16 or more), obstruction site (UPJ or proximal to UPJ, distal to UPJ) and treatment pre-treatment, post-treatment state). The split rena1 function ratio(%) of Tc-99m MAG, was well correlated with that of Tc-99m DTPA (r=0.92). There was no statistically significant difference between time parameters of Tc-99m MAG, and Tc-99m DTPA. The image quality of Tc-99m MAG, was superior to that of Tc-99m DTPA (p<0.05). In conclusion, Tc-99m MAG, renal scan had a superior image quality to that of Tc-99m DTPA and correlated well with other Tc-99m DTPA parameters. Thus, Tc-99m MAG, renal scan may be considered to replace Tc-99m DTPA in the evaluation of patients with upper urinary tract obstruction.
Constriction, Pathologic ; Female ; Humans ; Male ; Pentetic Acid* ; Ureter ; Urinary Tract*

PURPOSE: The present paper reports a systematic study on the effect of bifunctional chelators (BFC) namely, NOTA, DOTA, and DTPA, on the radiochemical formulation, in vitro stability, and in vivo biological properties of ⁶⁸Ga-labeled RGD peptide derivatives.METHODS: The three RGD conjugates namely, NOTA-Bn-E-[c(RGDfk)]₂, DOTA-Bn-E-[c(RGDfk)]₂, and DTPA-Bn-E-[c(RGDfk)]₂ were radiolabeled with ⁶⁸Ga and the radiolabeling was optimized with respect to the ligand amount, radiolabeling time, and temperature. Further, the ⁶⁸Ga complexes were assessed for their in vitro and in vivo stabilities. The biodistribution studies of the three radiolabeled conjugates were carried out in C57BL/6 mice bearing melanoma tumor at 30 min and 1 h post-adimistration.RESULTS: NOTA-Bn-E-[c(RGDfk)]₂ could be radiolabeled with ⁶⁸Ga at room temperature while DOTA-Bn-E-[c(RGDfk)]₂ and DTPA-Bn-E-[c(RGDfk)]₂ were radiolabeled at high temperature. ⁶⁸Ga-NOTA-Bn-E-[c(RGDfk)]₂ was found to be the most kinetically rigid in in vitro stability assay. The uptake of the three radiolabeled peptide conjugates in melanoma tumor was comparable at 1 h post-administration (NOTA; DOTA; DTPA (% I.D./g):: 2.78 ± 0.38; 3.08 ± 1.1; 3.36 ± 0.49). However, the tumor/background ratio of ⁶⁸Ga-NOTA-Bn-E-[c(RGDfk)]₂ was the best amongst the three radiotracers. ⁶⁸Ga-complexes of NOTA-Bn-E-[c(RGDfk)]₂ and DOTABn-E-[c(RGDfk)]₂ showed excellent in vivo stability while ⁶⁸Ga-DTPA-Bn-E-[c(RGDfk)]₂ showed significant metabolic degradation.CONCLUSION: These studies show that ⁶⁸Ga-NOTA-Bn-E-[c(RGDfk)]₂ would be the most appropriate ⁶⁸Ga-labeled radiotracer and the most amenable for kit formulation.
Animals ; Chelating Agents ; In Vitro Techniques ; Melanoma ; Mice ; Pentetic Acid ; Peptides

This study was designed to prospect the 'In-labelled paclitaxel as tumor imaging agent. In order to provide a taxol molecule with a functional group which is able to chelate In-lll, taxol-DTPA conjugate and 2-hemisuccinyltaxol were synthesized by esterification of taxol at C-2 on C-13 carbon with DTPA anhydride and succinic anhydride, respectively. Synthesis yield of the taxol derivatives was 34% for taxol- DTPA and 80% for 2'-hemisuccinyltaxol. Cytotoxicity of the taxol derivatives were measured by MTT method toward cell lines HT29, B16, P388, and CT26. The cytotoxic activities of the taxol derivatives were maintained, although less active than taxol. Radiolabelling of the taxol derivatives were proceeded directly with 111InCh or indirectly with 111In-citrate(ligand-exchange method). The ligand-exchane methocl was not suitable because some precipitat:es appeared during the reaction. On the contrary, by direct radiolabelling methnd, we were able to obtain taxol DTPA-111In in 100% radiochemical yield. However, 2'-hemisuccinyltaxol was not labellecl by both methods. Yield and radiochemiral purity of the radiolabelled com- pound were determined by HPI.C, paper chromatography and instant thin layer chromatography. Taxol-DTPA-111In was characterized to be hydrophilic by lipophi- licity test, and nearly non-adhesive to HT29, E316, P388, and CT26 by cell hinding affinity test. Binding affinity of the taxol-DTPA-111In complex to serum proteins was also examined by protein precipitation with 30% trichloroacetic acid. The results showed that 309o of the taxol-DTPA-111In complex binds with serum proteins.
Blood Proteins ; Carbon ; Cell Line ; Chromatography, Paper ; Chromatography, Thin Layer ; Esterification ; Paclitaxel* ; Pentetic Acid ; Trichloroacetic Acid

PURPOSE: Chitin and chitosan are nontoxic natural chelators that chelate radiostrontium effectively. The purpose of this study was to compare radiostrontium chelation of chitin and chitosan with that of well known chemical chelators, namely EDTA and DTPA. MATERIALS AND METHODS: The chelaton rates of chitin, chitosan, EDTA and DTPA were compared using a column chromatography method (Sephadex G-25M, Sweden). Three kinds of chitins and four kinds of chitosans were used. All of them were water soloble. RESULTS: Phosphated chitosan showed the highest chelation yield of more than 97% at pH 7. All of chitins, chitosans, EDTA and DTPA showed chelation yield of more than 90% independent of varing pH level. CONCLUSION: Chitin and chitosan have similar chelation rate as compared with EDTA and DTPA.
Chelating Agents ; Chitin* ; Chitosan* ; Chromatography ; Edetic Acid* ; Hydrogen-Ion Concentration ; Pentetic Acid*