Objective: To examine the prevalence and risk factors of criminal behavior among persons with schizophrenia in a rural area. Methods: A 14-year prospective follow-up study (1994 - 2008) was conducted on criminal behavior, clinical symptoms, social functioning, treatment and caring status among 510 persons with schizophrenia in Xinjin County, Chengdu. The instruments included the Present State Examination (PSE-9), Social Disability Screening Schedule (SDSS), and Positive and Negative Syndrome (PANSS) .Results: Totally 489 patients (95.9%) were followed up. The life-time prevalence of criminal behavior among these patients was 13.5%. Criminal behavior was more likely to happen among the persons who were unmarried (19.1%), younger t (41.2 ± 16.6) years old], and had earlier onset age [(28.4 ± 10.4) years old] .In the follow-up data, the rates of patients'criminal behavior were relatively higher among these patients who had poor family economic status (16.6%), no caregiver (28. 6%), homelessness (25.0%), and higher positive (14.1 ± 7.4), negative (19.3±10.2) and total scores (68.7±28.7) of PANSS (P<0.05) .Conclusion: The criminal behavior in patients with schizophrenia is common in rural areas and may be affected by multi-factors. The risk factors of criminal behavior in persons with schizophrenia, such as their marriage, family intervention and social support services, should be considered for community mental health policy making, community mental health services and family interventions.

Purpose To investigate the diagnosis,differential diagnosis and clinical manifestation of primary plasma cell leukemia (PPCL) and lymphoma with increased plasma cell.Methods Through clinical data and cell morphology,flow cytometry (FCM),immunofixation electrophoresis and immunohistochemistry of EliVision two-step examination were used to analyze 7 cases of PPCL and 3 cases of lymphoma with increased plasma cell.Results All patients with PPCL and lymphoma with increased plasma cell presented with anemia,thrombocytopenia,fever,liver and spleen and lymph node swelling.The proportion of plasma cells in peripheral blood morphology were larger than 20％,accompanied by morphological abnormality.FCM of peripheral blood showed all 7 cases of PPCL expressed CD38 and CD138,CD56 expression in the 2 cases and CD20 in the 2 cases.The light chain (Lamda,Kappa) showed a monoclonal restricted expression,which was consistent with the diagnosis of PPCL.CD19 and CD45 were weakly positive in 3 cases of lymphoma with increased plasma cell,CD38 and CD138 were positive,and no restricted expression was found in light chain IgL,wich belonging to the immunophenotypes of normal plasma cells.Of 3 cases of light chain (Ig) without restrictive expression,2 of them were angioimmunoblastic T-cell lymphoma (ATCL) and 1 case was CD30-positive sinusoidal large B-cell lymphoma (CD30 + SLBCL) that confirmed by lymph node biopsy and pathological examination.Conclusion The PPCL and lymphoma with increased plasma cell have the same clinical manifestations and similar morphological characteristics of blood cells.The diagnosis of PPCL should be combined with immunoelectrophoresis and FCM,and the diagnosis of lymphoma with increased plasma cell needs to be confirmed by histological examination of lymph nodes.

Objective To investigate the anti-tumor activities of cell-penetrating peptide ( CPP) - mediated trichosanthin ( TCS) , which is a recombinant protein obtained from Radix Trichosanthis. Methods Cysteine residue was introduced to the C-terminus of TCS by protein recombinant technique, and then with the newly-formed terminal as the modification site, TCS was coupled with CPP. As a target protein, CPP-mediated TCS was isolated and purified by affinity chromatography. The expression of the target protein and its responsiveness to reducing substances were detected by using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The cellular uptake rate of CPP-mediated TCS was determined by using cell uptake test, and its anti-tumor activity was measured by using methyl thiazolyl tetrazolium (MTT) assay. Results The TCS-CPP compound had been successfully developed in this study, and showed certain reducing responsiveness. After modified with CPP, TCS had higher cellular uptake rate and stronger anti-tumor effect on HeLa and MCF-7 cells. Conclusion TCS modified by CPP can enhance the anti-tumor activities of TCS.

Abstact:Objective To study the relationship between acute mountain sickness and geographic factors in young males,and provide no-vel materials for screening susceptible population and exploring novel research ideas and methods. Methods Total 294 young men came from low altitude were randomly selected,questionnaire was conducted and military AMS standard was applied for diagnosis. Binary logistic re-gression was used to analyze the relationship between 10 different geographical factors and AMS incidence and figure out the risk factors for medical geographical differences of AMS. Results AMS incidence was 52. 04% (153 people),with most commonly seen dizziness,head-ache,shortness of breath,chest tightness and lip empurples,the occurrence was 89. 54%,84. 31%,66. 01%,65. 36% and 60. 78%,respec-tively. Binary logistic regression analysis revealed that altitude was the only geographical risk factor (OR=0. 998,P<0. 05). Conclusion Among the population who entered plateau,lower native altitude they used to live brings higher risk of AMS,therefore corresponding preven-tions should be adapted for those who come to high altitude from low altitude in order to ensure their health and safety.

Objective To study the mechanism of RING finger protein 34 ( RNF34 ) involved in innate immunity . Methods Recombinant PCR was used and transient expression of the plasmid was achieved in HEK 293T cells.The cells were stimulated with Sendai virus ( SeV) or N-RIG-Ⅰfor the indicated time while luciferase activity was observed using the dual-luciferase reporter assay kit .Results We constructed the plasmid pcDNA 3-Flag-RNF34 and its three mutations .The study found that when stimulated by SeV , RNF34 could inhibit the activity of NF-κB and IFN-βmore significantly than RNF34-ΔFYVE, RNF34-ΔCID and RNF34-ΔRING.We also found that RNF 34 and its three mutants had similar inhibitory effect when the activation of NF-κB and IFN-βwas stimulated by the N-RIG-Ⅰ.Conclusion RNF34 negatively regulates innate immunity by acting on the RIG-Ⅰ-MAVS signaling pathway .

Objective To make the chromosome karyotype analysis of 130 patients with leukemia by using the improved chromosome short-term culture method.Methods We optimized the main factors with a single factor gradient experiment in short-term culture of bone marrow chromosome, including colchicines concentration, duration of action of colchicines,and hypotonic time.On this basis,we conducted the three-factors and three-level orthogonal experiment to achieve improved bone marrow chromosome preparation system,which was later applied in 130 patients with leukemia in our hospital.Results The orthogonal experiment results showed that the optimum conditions were colchicines concentration of 0.07 μg/mL,colchicines action time of 80 min,and hypotonic time of 35 min during the preparation of the bone marrow chromosome.Using this method,the chromosome preparation success rate reached 97.69% and the detection rate of abnormal karyotype reached 82.3% in the chromosome karyotype analysis.Conclusion Bone marrow chromosome preparation system with colchicines concentration of 0.07 μg/mL and colchicines action time of 80 min,and hypotonic time of 35 min is worthy of clinical promotion.

Objective To investigate the effects of different doses of oxaliplatin (L-OHP) chemotherapy regimen for treatment of colorectal cancer and its influence on the adverse reactions in the nervous system.Methods A total of 92 cases of colorectal cancer patients in Wuhan Wuchang Hospital from August 2013 to April 2016 were selected as the research objects, which were randomly divided into the observation group (46 cases) and the control group (46 cases) according to the random lottery envelopes. All the patients were given FOLFOX chemotherapy. The observation group was treated with small dose L-OHP chemotherapy(60 mg/m2),the control group was treated with routine dose of L-OHP chemotherapy(120 mg/m2), and the efficacies were evaluated after 3 cycles. Results All the patients completed chemotherapy. The observation group included 26 cases of complete remission(CR),11 cases of partial remission(PR),5 cases of stable disease (SD), 4 cases of progression of disease (PD). The control group included 28 cases of CR, 10 cases of PR, 3 cases of SD, 5 cases of PD. Overall response rate (ORR) in the observation group and the control group were 80.4 % (37/46) and 82.6 % (38/46) respectively, and there was no significant difference between the two groups (χ 2= 0.072, P > 0.05). Nervous system adverse reactions included 6 cases of gradeⅠ, 3 cases of grade Ⅱ, 1 case of grade Ⅲ in the observation group, and 12 cases of grade Ⅰ, 6 cases of gradeⅡ,4 cases of gradeⅢin the control group.The incidence rate in the observation group was lower than that in the control group [21.7 % (10/46) vs. 47.8 % (22/46), χ2= 6.900, P < 0.05]. Up to November 2017, PD and overall survival time in the observation group were longer than those in the control group [(19 ±4)months vs. (16±4) months, t= 4.314, P< 0.05; (24±4) months vs. (20±5) months, t = 4.170, P < 0.05]. Conclusion The low dose L-OHP chemotherapy regimen has a favorable effect in patients with colorectal cancer, and it can reduce the adverse reactions in the nervous system and prolong the survival time of patients.

Objective To propose an optimal therapeutic scheme, finite element analysis was used to evaluate the biome?chanical status of femoral head under daily activities. Methods Non?necrotic femoral head volunteers (11 people) and necrotic femoral head patients (7 cases) were included in this study. All these participants were divided into two groups:control group and abnormal group. The DICOM imaging files of each participant were imported into ScanIP software to form a mask showing the prox?imal femurs. Then the finite element models of proximal femurs were progressively reconstructed. The generated models were im?ported into Abaqus, and the forces were added on these models to simulate daily activities, including standing, sitting, upstairs, and squatting etc. The loads and boundary conditions of digital daily activities simulation were collected from Orthoload database. All of the femoral finite element models were progressed in Abaqus under above activity conditions. After the models were pro?cessed, stress concentrating region was defined in each models respectively, which expressed the main bearing structure in each model based on the result of finite element analysis. At last, the elastic modulus (E?modulus) and strain of the stress concentrating region in each femoral model were extracted and calculated. Results There was no significant difference in age, weight, and height between these two groups. In control group, no significant differences of E?modulus (7 125±745 MPa on left, and 7 112±751 MPa on right) and strain (micro?strain:952 on left, and 954 on right)were found between their bilateral proximal femurs;however in abnormal group, the patients' E?modulus (5 798±1036 MPa on normal side, and 6 667±888 MPa on necrotic side) and strain (mi?cro?strain:1 261 on normal side, and 987 on necrotic side) of necrotic side were significantly lower than normal side. As to all the daily activities which were considered in this study, in abnormal group, the E?modulus among each postures of abnormal side were significantly lower than the E?modules of healthy side;the micro?strain in downstairs was also significantly lower than other pos?tures. Conclusion Daily activities with different intensity could generate different mechanical effects on femoral head, espe?cially on necrotic femoral head. The higher intensity of posture, the greater damage would be found in necrotic femoral head.

[Objective]To discuss the clinical effect of fludarabine and busulfan (Bu+Flu) as a low toxicity myeloablative conditioning regimen for allogeneic peripheral blood stem cell transplantation (allo-HSCT)in leukemia patients.[Methods]Clinical data of 13 patients with hematological malignancies receiving conditioning regimen with Bu+Flu for allo-HSCT were analyzed retrospectively.Conditioning regimen was Bu+Flu,compalriot mismatched and unrelated transplantation combined with rabbit anti-human thymocytes immune globulin (ATG).CsA+short course of methotrexate or CsA + mycophenolate mofetil were used to prevent graft-versus-host disease (GVHD).DNA sequencing of short tandem repeat (STR)polymorphism analysis method was performed for identification of donor stem cells implantation.[Results]13 patients all tolerated with this conditioning regimen well,no serious complications occurred.Neutrophil engraftment was at 9-15 days (median 11 days),platelet engraftment at 8-25 days (median 13 days).10 patients achieved hematopoiesis reconstitution with their full donor chimerisms confirmed by STR-DNA analysis.Acute GVHD occurred in 5 cases,accounting for 38.5％.Chronic GVHD occurred in 4 cases of 10 patients could be assessed,accounting for 40.0％.Severe GVHD more than Ⅱ degree did not happen.1-39 months (median time 11 months)of follow-up revealed the overall survival rate of 76.9％(10/13),disease-free survival of 61.5％ (8/13).The causes for death were relapse in all.[Conclusion]The conditioning regimen with Bu+Flu has low toxicity,well tolerance and better effect.

Objective To investigate the clinical characteristics of skull cranial langerhans cell histiocytosis in children and improve clinical understanding and therapeutic effect Methods A retrospective clinical study was conducted in 29 cases of skull cranial langerhans cell histiocytosis treated in Shengjing Hospital Affiliated to China Medical University from January 2012 to January 2018. Results The main lesion of skull bone was dissolved bone. Among them, there were 5 cases of frontal bone lesions, 10 cases of temporal bone lesions, 2 cases of occipital lesions, and 12 cases of multiple lesions in the skull;There were 10 cases of vertebral lesions and 5 cases of multiple bone lesions involved around the body. Other clinical manifestations included insipidus in 4 cases, erythra in 3 cases, hepatosplenomegaly in 2 cases and lymphadenectasis in 1 case. All cases were followed up for 0.5 to 5.0 years. In the group of combined surgery and chemotherapy treatment, the prognosis of single system involvement cases was that 9 patients were cured, 2 patients were stable and 1 patients were progressing, while the prognosis of multiple systems involvement cases was that 1 patient was cured, 2 patients were stable and 3 patients were progressing. The prognosis of patient with multiple systems lesions was relatively poor compared to that of the cases of single system lesion and there was statistical difference. In the pure operation group , the prognosis of single system involvement cases was that 5 patients were cured, 1 patient was stable and 1 patient was progressing, while the prognosis of multiple systems involvement cases was that 1 patient was stable and 3 patients were progressing. Conclusions Children with skull cranial langerhans cell histiocytosis present single or multiple cranial osteolytic lesions. It is often accompanied by multiple systems lesions around the body, and prognosis of children with multiple systems lesions was is relatively poor.