1.Toll like receptor expression in spleen of rat skin transplantation model
Wei ZHANG ; Pengxiang XU ; Shikun QIAN
Chinese Journal of Immunology 2015;(9):1229-1234
Objective:The expression patterns of TLR4,9,10 in the rat spleen were compared between the rat model of acute skin-transplant rejection and physiologically normal rats ,and their physiological significance were accessed.Methods:6 Wistar and 6 SD rats were used as donors ,and another 24 SD rats were used as recipients which were divided into 4 groups of 6.The same back skin slices were transplanted from donor to recipient , Group A:allograft ( Wistar to SD );Group B:homograft ( SD to SD );Group C:autograft;and Group D:normal SD rats as control.Rat spleens were collected at 1,4,7,10,14,and 21 days after surgery ,and mRNA levels of TLR4,9,10 in splenic mononuclear cells were determined by real-time PCR and corresponding protein expression was accessed by immunohistochemistry.Results:TLR4,9,10 mRNA levels of Group A were significantly higher than that of Group B and C ( P<0.05);TLR4,9,10 mRNA levels showed no significant difference between Group B and C (P>0.05).TLR4,9,10 expressions were moderate (++) to strong (+++) in Group A,and generally weak (+) in Group B,C and D.Conclusion:The patterns of TLR4,9,10 mRNA and protein expression in the rat model of acute skin-transplant rejection indicate that the three genes are involved in acute transplant rejection;the three genes may be expressed coordinately;inhibition of the Toll-like receptors may provide therapeutic targets for acute transplant rejection.
2.Bronchiolitis Obliterans Syndrome after Hematopoietic Stem Cell Transplantation Treated by WANG Shu-chen with Tongue Picture as the Key Link
Wei ZHOU ; Changzheng FANG ; Qing MIAO ; Lixia CAO ; Pengxiang XU
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(5):119-121
This article introduced professor WANG Shu-chen's experience in picture treating bronchiolitis obliterans syndrome (BOS) after hematopoietic stem cell transplantation with tougue picture as the key link. Patients with BOS showed deficiency of essence of tongue crack, which should be treated by warming kidney yang, replenishing essence and marrow; fester tongue for qi disorder, inflammation caused by fire, which should be treated by regulating qi, and clearing heat; exfoliative fur accumulation, which should be treated with blood stasis and toxin, removing blood stasis and toxin; thick and greasy fur, which should be treated by warming spleen and activating spleen.
3.Establishment of a universal fluorescent multiplex cPCR method for detection of copy number variations in mice
Tianzhu CHAO ; Pengxiang LI ; Fuyi XU ; Kai LI ; Yuxun ZHOU ; Junhua XIAO
Acta Laboratorium Animalis Scientia Sinica 2015;(6):591-596
Objective To establish a high throughput general multiple competitive polymerase chain reaction ( cPCR) detecting method of copy number variations ( CNVs) for the population of chromosome 1 substitution strains from wild mice.Method The selected 14 loci, including 11 CNVs on chromosome 1 and internal control loci on other three chromosmes (Chr 7, Chr 19 and Chr X), were detected based on the universal fluorescent primer multiple competitive pol-ymerase chain reaction.All specific cloned plasmids were constructed as competitors.Results Altogether 11 CNVs were designed in one panel, and the copy of Chr X accurately reflects the gender.Conclusions A rapid and high-throughput fluorescent multiplex cPCR assay is established which can be used for detection of copy number variations on chromosome 1 in mice.
4.Finite Element Analysis on Biomechanical Properties of Unicompartmental Knee Arthroplasty in ACL Fracture of the Knee Joint
Pengxiang LI ; Gaiping ZHAO ; Feiyi XIA ; Jiajing YANG ; Haifei XU ; Tong MA ; Yihui TU
Journal of Medical Biomechanics 2020;35(1):E070-E076
Department of Orthopedics, Yang Pu Hospital Affiliated to Tongji University,
5.Differences of the structure, succession and function of Clostridial communities between jiupei and pit mud during Luzhou-flavour baijiu fermentation.
Wei QIAN ; Zhenming LU ; Lijuan CHAI ; Xiaojuan ZHANG ; Pengxiang XU ; Qi LI ; Songtao WANG ; Caihong SHEN ; Jinsong SHI ; Zhenghong XU
Chinese Journal of Biotechnology 2020;36(6):1190-1197
Clostridia inhabiting in jiupei and pit mud plays key roles in the formation of flavour during the fermentation process of Luzhou-flavour baijiu. However, the differences of Clostridial communities between jiupei and pit mud remains unclear. Here, the species assembly, succession, and metabolic capacity of Clostridial communities between jiupei and pit mud were analysed by high-throughput sequencing and pure culture approaches. The ratio of Clostridial biomass to bacterial biomass in the pit mud was relatively stable (71.5%-91.2%) throughout the fermentation process. However, it varied widely in jiupei (0.9%-36.5%). The dominant Clostridial bacteria in jiupei were Clostridium (19.9%), Sedimentibacter (8.8%), and Hydrogenispora (7.2%), while Hydrogenispora (57.2%), Sedimentibacter (5.4%), and Caproiciproducens (4.9%) dominated in the Clostridial communities in pit mud. The structures of Clostridial community in pit mud and jiupei were significantly different (P=0.001) throughout fermentation. Isolated Clostridial strains showed different metabolic capacities of volatile fatty acids in pure culture. Spatial and temporal heterogeneity of Clostridial communities existed in the baijiu fermentation pit, which was closely related to the main flavour components of Luzhou-flavour baijiu.
Alcoholic Beverages
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microbiology
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Bacteria
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classification
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metabolism
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Clostridium
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physiology
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Fatty Acids, Volatile
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metabolism
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Fermentation
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Food Microbiology
6.Interpretation of Novel Coronavirus Infection :Expert Consensus on Guidance and Prevention Strategies for Hospi tal Pharmacists and the Pharmacy Workforce
Rongsheng ZHAO ; Yiheng YANG ; Li YANG ; Zijian LI ; Fang LIU ; Zhenyu REN ; Wei LIU ; Zhanmiao YI ; Yingqiu YING ; Xiaoxiao LI ; Yingying YAN ; Huibo LI ; Shujie DONG ; Weilong SHI ; Xiaohan XU ; Pengxiang ZHOU ; Zaiwei SONG ; Siqian ZHENG ; Ying LIU ; Shen ZHOU ; Suodi ZHAI
China Pharmacy 2020;31(4):385-389
OBJECTIVE:To provid e reference for pharmaceutical workers to better understand Novel Coronavirus Infection : Expert Consensus on Guidance and Prevention Strategies for Hospital Pharmacists and the Pharmacy Workforce (hereinafter referred to as “expert consensus ”),and to apply and practice in specific work ,so as to give full play to the role of pharmacists to help fight the epidemic.METHODS :The background of the formulation and revision of the expert consensus were introduced ,and its main contents and viewpoints were interpreted. RESULTS & CONCLUSIONS :The text of expert consensus is divided into 8 parts,mainly including disease diagnosis and treatment [SARS-CoV- 2 infection related background ,clinical manifestations and diagnosis, treatment],hospital pharmacy (prevention and control strategy ,work guidance ),drug and facility support management(key drug/facility/equipment support ,management and use of the drug in special circumstances ),information sources and related resources ,etc.,which comprehensively and detailedly provide information ,guidance and strategies for coronavirus SARS-CoV-2 infection prevention and control to play the role of pharmacists in hospital pharmacy well ,do well in the protection of staff in different pharmaceutical posts ,drug security work in response to epidemic situation ,and develop pharmaceutical care. So far,the understanding of SARS-CoV- 2 in the pharmaceutical industry is relatively limited. Based on the accumulated experience and progress in epidemic prevention and control ,the expert consensus will be updated and improved continuously ,so as to provide guidance and help for hospital pharmaceutical personnel.