Objective To explore the application value of Carbon Nanoparticles followed by microscale Methylthioninum Chloride in sentinel lymph node biopsy for breast cancer.Methods Two hundred and eight breast cancer patients were selected in the First Affiliated Hospital of Zhengzhou University during recent two years who performed sentinel lymph node biopsy.They were randomly divided into five groups according to different lymphatic tracer injected:Sequential group (A group,128 patients),Carbon Nanoparticles group (B group,44 patients) and Methylthioninum Chloride group (C group,36 patients).According to different Methylthioninum Chloride doses,sequential groups were randomly divided into low dose group (A1 group,48 patients),middle dose group (A2 group,42 patients),high dose group (A3 group,38 patients),If sentinel lymph nodes were negative no longer performed axillary lymph nodes dissection.Receive the five groups statistics of detection rate,the condition of lymphatic tracer,the incidence of local skin irritation and so on.Results (1) The detection rate of A,B and C three groups had no obvious difference.The incidence of lymphatic tracer dyed not ideal in B group was higher than A and B two groups,the incidence of local skin stimulation in C group was higher than A and B two groups.(2) A1,A2,A3,three groups had no difference of the detection rate and lymphatic tracer,but the incidence of local skin stimulation in A3 group is higher than A1,A2 two groups.3.5 groups of SLN detection rate had no relationship with patients' age,BMI,tumor size,tumor location,tumor molecular classification factors (P ＞ 0.05).Conclusions Carbon Nanoparticles followed by microscale Methyhhioninum Chloride detection rate was similar with two separate application,but can reduce the lymphatic vessels not developing situation when used Carbon Nanoparticles alone and reduce the local irritation caused by large dose of Methylthioninum Chloride,so it can be a good choice of SLNB in clinical.

Lymphedema has a high incidence and lacks sustainable and effective treatment. Recently, with the development of tissue engineering and the popularization of interdisciplinary treatment, the lymphatic network can be reconstructed with lymphoid stem cell regenerative technology and tissue engineering technology combined with decellularized scaffolds. The treatment and prevention of lymphedema have recently achieved initial success in experiments. The long-term efficacy still needs to be verified by further investigations. The existing literature concerning treatment for lymphedema by stem cells and tissue engineering is critically presented and summarized in terms of the physiological characteristics of lymphatic vessels, pathological mechanism of lymphedema, application of stem cell technology in the treatment of lymphedema, and lymphatic tissue engineering. With the in-depth research on lymphatic regenerative medicine technology and lymphatic tissue engineering, the desire to successfully treat and prevent lymphedema through lymphatic regenerative medicine technology and tissue engineering can be realized.

Lymphedema has a high incidence and lacks sustainable and effective treatment. Recently, with the development of tissue engineering and the popularization of interdisciplinary treatment, the lymphatic network can be reconstructed with lymphoid stem cell regenerative technology and tissue engineering technology combined with decellularized scaffolds. The treatment and prevention of lymphedema have recently achieved initial success in experiments. The long-term efficacy still needs to be verified by further investigations. The existing literature concerning treatment for lymphedema by stem cells and tissue engineering is critically presented and summarized in terms of the physiological characteristics of lymphatic vessels, pathological mechanism of lymphedema, application of stem cell technology in the treatment of lymphedema, and lymphatic tissue engineering. With the in-depth research on lymphatic regenerative medicine technology and lymphatic tissue engineering, the desire to successfully treat and prevent lymphedema through lymphatic regenerative medicine technology and tissue engineering can be realized.

Objective:To summarize the initial experience on da Vinci robot breast surgery (RBS).Methods:The clinical data of 151 patients who underwent RBS-related surgical procedures at the First Affiliated Hospital of Zhengzhou University from Sep 2022 to Jan 2024 were retrospectively analyzed.Results:Forty patients underwent surgery using the da Vinci robot Si TM system, and 137 patients underwent surgery using the da Vinci robot Xi TM system. The postoperative follow-up time was (3±1) month,There was no necrosis of the nipple-areola complex and no serious perioperative complications in the patients except for 3 case of prosthesis loss.Most patients were satisfied with the postoperative aesthetic results. Conclusions:Although the currently available evidence is immature and the follow-up time is short, our preliminary results suggest that RBS provides better cosmetic results and may reduce the risk of nipple necrosis.

Objective:To explore the effect of da Vinci robotic nipple sparing mastectomy and immediate breast reconstruction with gel implant in breast cancer.Methods:From September 2022 to May 2024, 116 female patients with breast cancer, aged 17-58 (42.1+ 7.2) years, with a body mass index (22.93+ 2.53) kg/m 2, who underwent unilateral da Vinci robotic nipple-areola preserving immediate prosthetic breast reconstruction in the Department of Breast Surgery, the First Afiliated Hospital of Zhengzhou University, and who fulfilled the inclusion criteria. were enrolled in this study. The clinicopathologic characteristics, robotic microscopy time, total operative time, and perioperative complications of the patients were collected and analyzed to determine the effectiveness and usefulness of the procedure. Results:The da Vinci robotic SI system was utilized in 14 patients, while the da Vinci robotic X system was employed in 102 patients. A total of 78 three-port, three-arm operations and 38 single-port. three-arm operations were conducted. The mean robotic operating time and total operating time were (115.95+ 58.10) minutes and (205.14+ 57.72) minutes, respectively. Additionally, the robotic preparation time was reduced from the initial 25 minutes to the current 5 minutes. At the postoperative follow-up time of three months, no nipple-areola complex necrosis was observed, and no serious perioperative complications were identified in 109 patients, with the exception of two cases of implants loss and five cases of heat steam induced skin damage. With the exception of two cases of prosthesis loss and five instances of thermal skin injury, 109 patients exhibited no significant perioperative complications and expressed satisfaction with the postoperative aesthetic outcomes.Conclusions:The da Vinci robotic nipple sparing mastectomy and immediate breast reconstruction with gel implant has the potential to provide superior aesthetic outcomes without significant perioperative complications for the female patients.