Enzyme-linked immunosorbent assay ( ELISA) was applied to detect the antibody against CoxA16 and EV71 among 0~14 age groups. In 1 000 cases of children, the total positive rate of anti-EV71 IgM was 19. 9%, the total positive rate of anti-EV71 IgG was 47. 7%, the total positive rate of anti-CoxA16 IgM was 22. 8%, the to-tal positive rate of anti-CoxA16 IgG was 51. 3%. There was a negative correlation between positivity for EV71 IgM and positivity for CoxA16 IgM(χ2 =2. 794,P>0. 05). There was also a negative correlation between positivity for EV71 IgG and positivity for CoxA16 IgG(χ2 =2. 793,P>0. 05). The positive rate of IgG antibody against CoxA16 and EV71 was higher in neonatal group in which anti-EV71 IgG was 41 . 5% and anti-CoxA16 IgG was 49 . 5%. The lowest positive rate of IgG antibody against CoxA16 and EV71 was in infant group in which anti-EV71 IgG was 38. 0% and anti-CoxA16 IgG was 43. 5%. Except for neonatal group,the positive rate of IgG antibody against Cox-A16 and EV71 increased gradually with the age growth, there were statistical differences among the age groups (χ2=27. 04 and 19. 98,P<0. 01). With the age growth,the positive rate of IgM antibody against EV71 and CoxA16 increased,there were statistical differences among the age groups(χ2 =41. 12 and 37. 13,P<0. 01). There were not statistical differences between gender groups (χ2 =0. 51,1. 77,0. 36 and 2. 12,P>0. 05).

Object To study the chemical constituent and the bioactivity in the bark of Tsoongiodendron odorum Chun Methods By bioactive following method, the extracts of both EtOAc and n BuOH in the bark of T odorum were screened for in vitro anti tumor activity Results Five constituents were obtained Among them, three from EtOAc fraction belonged to germacranolides They were costunolide (Ⅰ), parthenolide (Ⅱ) and dihydroparthenolide (Ⅲ) The other two were from the fraction of n BuOH, one was an oxoaporphinoid alkaloid, liriodenine (Ⅳ), and the last was a furanone, 2, 3 dihydroxyl 2 methyl butylrolactone (Ⅴ) Conclusion All the above five compounds are found for the first time from this plant Compounds Ⅰ, Ⅱ, Ⅳ, and Ⅴ show the cytotoxic activities against a variety of tumor cell strains, respectively

Objective To establish and evaluate a rat model of adriamycin-induced myocardial injury.Methods Tweenty-four male SD rats were randomly divided into two groups. The adriamycin (ADR) group (n=15) was injected intraperitoneally with six equal doses of ADR (2 mg/kg each time, with a cumulative dose of 12 mg/kg) over a period of two weeks, while the control group (n=9) received an equivalent volume of normal saline intraperitoneally alone. The Rats were observed for 5 weeks after treatment. The general conditions and mortality were recorded. At the end of experiment, the parameters of hemodynamics including LVSP, LVEDP, ±LVdP/dtmax and HR were measured. The pathological changes were analyzed by histological hematoxylin-eosin staining. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activity as well as malondialdehyde (MDA) content in heart tissue were measured according to the kit instructions, respectively. Results The cumulative mortality in the ADR group was 40%. Compared with the control group, LVEDP and -LVdP/dtmax were significantly increased in the ADR group (P<0.001, P<0.05). The results of pathological examnation of the ADR group were consistent with characteristic changes of myocardial injury. Myocardial malondialdehyde (MDA) content was significantly increased and glutathione peroxidase (GSH-Px) activity decreased in the ADR group compared with those in the controls.Conclusion A rat model of adriamycin-induced myocardial injury has been successfully established by administration of ADR (cumulative dose, 12 mg/kg) to rats in six equal intraperitoneal injections over a period of 2 weeks, resulting in cardiac dysfunction and histological damages.

BACKGROUND:Kinesin II family member 3A (KIF3A), as an important member of the kinesin-2 family, not only holds some general characters of kinesin including the regulation of intracel ular transport and mediation of microtubule movement, but also possesses its own specific features.
OBJECTIVE:To review the research progress of the function of KIF3A recently.
METHODS:A computer-based online research for relative literatures published from January 1996 to July 2016 was performed in PubMed and CJFD databases using the English retrieval words of“KIF3A;kinesin;molecular motor”and Chinese keywords of“kinesin;microtubule;function;molecular motor;spinal cord injury”, respectively. A total of 92 articles were retrieved, and 62 eligible articles were included according to the inclusion criteria.
RESULTS AND CONCLUSION:KIF3A is involved in new bone formation regulation, in the control of nephron number, cell survival and gene expression, in sperm formation and in the inhibition of prostate cancer and glioblastoma process:In the meanwhile, whether KIF3A is involved in spinal nerve injuries is discussed. All provide valuable new information and new ideas for further in-depth study on the KIF3A.

A quantitative analysis method of multi-components with a single marker (QAMS) for simultaneous determination of six marker compounds (one from phenolic acids and five from phthalides) in Chuanxiong Rhizoma was established by applying HPLC and using butylidenephthalide as the internal reference substance. And also the feasibility and accuracy of the established method for quality evaluation and application of Chuanxiong Rhizoma were investigated and validated. The analysis was performed with the mobile phase consisting of acetonitrile - 0.2% aqueous formic acid. The flow rate was 1.0 mL . min-1 and the column temperature was maintained at 30 °C. The detection wavelengths were set at 252 nm (for ferulic acid, Z-ligustilide, and butylidenephthalide) and 266 nm (for senkyunolide I, senkyunolide A, and coniferyl ferulate), separately, and 20 µL was injected for analysis with gradient elution. The results showed that there were no significant differences observed between the HPLC-QAMS method and the external standard method (RSD <5%). The relative correction factors were credible (RSD < 5%) in changed chromatographic conditions. The established HPLC-QAMS method can be accurately used for simultaneously evaluating and controlling the quality of Chuanxiong Rhizoma with multi-components.

Objective To investigate the value of ATP stress 99Tcm-MIBI G-MPI in evaluating instent restenosis.Methods Seventy-one patients (56 males,15 females,age range:37-83 (60.2±9.5) yeas) who had undergone coronary stent implantation at least 3 months ago,accompanying a typical angina pectoris symptom,were included in this perspective study.All patients underwent ATP stress 99Tcm-MIBI G-MPI and CAG (gold standard) within 1 month.The diagnostic efficiency of ATP stress 99Tcm-MIBI G-MPI on in-stent restenosis was evaluated.Fisher exact probability method was used to analyze data with SPSS 17.0.Results Five patients were excluded because of new stenosis.According to the results of CAG,39 patients with 45 coronary arteries showed in-stent restenosis among 66 patients with 99 coronary arteries.There were 19 patients with myocardial infarction among the 39 patients with in-stent restenosis.Contrarily,there were 16 patients with myocardial infarction among the 27 patients without in-stent restenosis.The diagnostic Se,Sp,Ac,PPV and NPV of ATP stress 99Tcm-MIBI G-MPI for in-stent restenosis were 85％(33/ 39),89％(24/27),86％ (57/66),92％ (33/36) and 80％ (24/30),respectively in 66 patients.Those were 79％(15/19),88％(14/16),83％(29/35),88％(15/17) and 78％(14/18) in patients with myocardial infarction,and 90％ (18/20),91％ (10/11),90％ (28/31),95％ (18/19) and 83％ (10/12) in patients without myocardial infarction (all P＞0.05).Conclusion ATP stress 99Tcm-MIBI G-MPI has high diagnostic efficiency in evaluating in-stent restenosis.

As a pilot program of teaching reform,some excellent undergraduates majoring in nursing were selected to take specialized courses in ICU in order to cultivate the intensive care specialized nurses.The teaching team of specialized courses in ICU was established to guarantee the teaching quality.The team was in charge of the ICU specialized curriculum building,textbooks selection and teaching content examination.The high leveled teaching team of specialized courses in ICU was organized by taking teaching and researching section as a unit to improve theoretical teaching level,using clinical department as training base to enhance practice skill and participating in special subject classes and in-service study to promote specialized quality.