1.metabonomics research on coronary heart disease patients of phlegm turbidity syndrome and qi deficiency syndrome.
Peng CHENG ; Ze-qi CHEN ; Dong-sheng WANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(2):193-197
OBJECTIVETo study the correlation between Chinese medical types of coronary heart disease (CHD) [i.e., phlegm turbidity syndrome (PTS) and qi deficiency syndrome (QDS)] and their metabolites.
METHODSRecruited were 65 CHD patients including 37 cases of PTS and 28 cases of QDS. Serum endogenous metabolites in the two syndrome types were determined by gas chromatograph-mass spectrometer-computer (GC/MS), and their differences between their metabolic profiles analyzed.
RESULTSMore than 100 chromatographic peaks were totally scanned. Chromatograms obtained was matched with mass spectrum bank, and finally we got the category contribution value of 46 kinds of substances. Results of MCTree analysis showed patients of PTS and patients of QDS could be effectively distinguished. Compounds contributing to identify the two syndromes were sequenced as serine, valine, 2 hydroxy propionic acid. Comparison of metabolites showed contents of serine and 2 hydroxy propionic acid were higher in patients of PTS than in patients of QDS (P<0.05).
CONCLUSIONThe differences in the metabonomics of CHD TCM syndrome types could provide material bases for TCM syndrome differentiation of CHD, indicating that metabonomics technologies might become a new research method for TCM syndrome typing.
Aged ; Asian Continental Ancestry Group ; Coronary Artery Disease ; Coronary Disease ; metabolism ; therapy ; Female ; Heart Diseases ; Humans ; Male ; Medicine, Chinese Traditional ; Metabolome ; physiology ; Metabolomics ; Middle Aged ; Qi ; Research ; Sputum ; Syndrome
2.Effect of Dachuanxiong Prescription on nerve cell injury of migraine rat
Gan YAO ; Cheng PENG ; Dong ZHOU ; Yita WANG
Chinese Traditional Patent Medicine 1992;0(12):-
Objective: To study the effects of Dachuanxiong Prescription(Rhizoma Gastrodiae, Rhizoma Chuanxiong, etc.) on nerve cell apoptosis of migraine model rat. Methods : To choose disease model of migraine with nitroglycerin by means of flow cytometry and transmission electron microscope. Results : Dachuanxing Prescription can adjust the abnormal ultra-structure of nerve cell of migraine model rats, decrease the incidence and the apoptosis percentage. Conclusion : Dachuanxing Prescription can block the nerve cell apoptosis of migraine model rat.
3.Protective Effect of Compatibilities of Ginsenosides Rg1 and Aconitine on Myocardial Cells of in vitro Cul-tured Heart Failure Model
Yanhong DONG ; Xiaofang XIE ; Xuemei LI ; Yaning ZHU ; Cheng PENG
China Pharmacy 2017;28(4):472-475
OBJECTIVE:To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocar-dial cell of in vitro cultured heart failure model. METHODS:The myocardial cells of neonate rat were grouped into normal control group,model group,positive control group(Deslanoside injection,1×10-7 mol/L),ginsenosides Rg1 group(1×10-8 mol/L),acon-itine group (1 × 10-9 mol/L) or their compatibilities groups (1∶1,2∶1,1∶2,V/V). Except for normal control group,other groups were given 0.8%pentobarbital sodium to induce heart failure model of myocardial cells. After modeling,each group was given rele-vant medicine for 1 h,and then the activities of T-ATPase,Ca2+-Mg2+-ATPase,Na+-K+-ATPase in cells were all detected. The activi-ties of acyl carrier protein(ACP)and lactate dehydrogenase(LDH),and the contents of brain natriuretic party(BNP),TNF-α and total glycogen were measured in cell culture fluid. RESULTS:Compared with normal control group, T-ATPase and Ca2+-Mg2+-ATPase activities were decreased significantly in model group;meanwhile,Na+-K+-ATPase activity was increased signifi-cantly,and ACP,LDH activities and BNP content in cell culture fluid were increased significantly(P<0.05). Compared with mod-el group,the activities of T-ATPase in treatment groups were increased significantly,while the activity of LDH in cell culture fluid was decreased significantly;when the volume ratio of ginsenoside Rg1 to aconitine was 2∶1,protective effect was the strongest;the activities of Na+-K+-ATPase in aconitine group and compatibility groups were all decreased significantly,with statistical signifi-cance(P<0.05). The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups,but the content of to-tal glycogen in cells and the TNF-α content of in cell culture fluid had no change (P>0.05). CONCLUSIONS:Compatibility of ginsenosides Rg1 and aconitine can improve ATPase activities and membranous permeability,regulate BNP secretion and protect myocardial cell of heart failure model,especially the compatibility of ginsenosides Rg1 to aconitine of 2∶1 ratio.
4.Experimental study of Dachuanxiong Decoction on injury induced by ischemia in nerve cells
Gan YAO ; Cheng PENG ; Dong ZHOU ; Yitao WANG ;
Chinese Traditional Patent Medicine 1992;0(04):-
AIM: To study the protective effects of Dachuanxiong Decoction (Rhizoma Chuan Xiong, Rhizoma Gastrodiae, etc) on injury induced by ischemia in nerve cells. METHODS:The level variation of activity and calcium ions of nerve cell were observed, choosing nerve cell ischemia model with hypoxia device and adapting confocal laser scanning microscopy and MTT method.. RESULTS:Dachuanxiong Decoction adjusted the abnormal structure of ischemia nerve cell, inhanced the survival rate and nerve call's, activity and decreased the concentration of calcium ions of nerve cell. CONCLUSION: Dachuanxiong Decoction blocked the calcium ion channels, nourished and protected nerve cells from ischemic injury.
5.Research advances in the effects of thrombospondin-1 on the progress of diabetic retinopathy
Le DONG ; Cheng Peng XUE ; Peng Zhi YOU
Recent Advances in Ophthalmology 2017;37(10):984-987
Thrombospondin-1,an important extracellular matrix glycoprotein,is recognized as an effective endogenous angiogenesis inhibitor and can affect and regulate the adhesion,motility and proliferation of endothelial cells,as well as closely correlated with the development and progress of tumor and neovascular diseases.In recent years,it has been found that Tsp-1 is associated with many pathologic changes in diabetic retinopathy and is critical for the maintenance of retinal vascular balance.And the mechanism of action of TSP-1 and its role in the development of DR will be reviewed in this paper.
6.Retrospective study on treating thoracolumbar fractures with video-assisted thoracoscopic surgery and traditional anterior approach surgery.
Ming PENG ; Xin-Feng CAO ; Guo-Dong PENG ; Xiao-Cheng MA
China Journal of Orthopaedics and Traumatology 2012;25(9):747-750
OBJECTIVETo investigate the feasibility and the efficacy of video-assisted thoracoscopic surgery in treating thoracolumbar fractures.
METHODSFrom October 2000 to December 2009, the data of 44 patients with thoracolumbar fractures were retrospetively analyzed. All patients were treated with anterior decompression, auto-iliac bone graft and anterior internal fixation system. They were divided into thoracoscopic group (23 cases, treated with video-assisted thoracoscopic surgery) and traditional group (21 cases, treated with traditional anterior approach surgery). In the thoracoscopic group, there were 15 males and 8 females with an average age of 41.4 years (ranged, 19 to 76); and in the traditional group, there were 14 males and 7 females with an average age of 39.3 years (ranged, 20 to 74). All patients were followed up from 6 to 36 months with an average of 18 months. The operative time, volume of the blood loss, the decreased value of the occupation ratio of spinal canal (OR), the corrected and loss degree of Cobb angle, the improved condition of ASIA classification were compared between two groups.
RESULTSIn traditional group, operative time, volume of the blood loss, the decreased value of the occupation ratio of spinal canal (OR), the corrected and loss degree of Cobb angle, the improved grade of ASIA classification were (150.0 +/- 19.4) min, (970.0 +/- 72.0) ml, (35.5 +/- 6.4)%, (25.1 +/- 4.8) degrees, (1.0 +/- 0.7) degrees, (1.8 +/- 0.9) grades, respectively; and in thoracoscopic group, the above items were (170.0 +/- 20.8) min, (650.0 +/- 65.4) ml, (33.2 +/- 8.0)%, (23.6 +/- 5.4) degrees, (1.1 +/- 0.8) degrees, (2.0 +/- 1.1) grades, respectively. There was significant difference in volume of the blood loss between two groups (P < 0.05); there was no significant difference in operative time, the decreased value of the occupation ratio of spinal canal (OR), the corrected and loss degree of Cobb angle,the improved grade of ASIA classification between two groups (P > 0.05). The rate of fusion of all patients was 100%.
CONCLUSIONCompared with the traditional anterior approach surgery, video-assisted thoracoscopic surgery has advantages of little incision,less blood loss, less trauma, can obtain same clinical outcome and is a safe,effective method in treating thoracolumbar fractures.
Adult ; Aged ; Female ; Humans ; Lumbar Vertebrae ; injuries ; surgery ; Male ; Middle Aged ; Retrospective Studies ; Spinal Injuries ; surgery ; Thoracic Surgery, Video-Assisted ; methods ; Thoracic Vertebrae ; injuries ; surgery
7.Efficacy of statin in treatment of patients with chronic obstructive pulmonary disease at stable stage
Wei DONG ; Ping KANG ; Xiangzhu ZHONG ; Lin TANG ; Jianfang CHEN ; Hongying CHENG ; Weifen PENG
The Journal of Practical Medicine 2015;(11):1835-1837
Objective To explore the therapeutic effects of statin in patients with chronic obstructive pulmonary disease (COPD) in stable period. Methods 83 patients with COPD in stable period were recruited and randomly divided into two groups, treatment group (n = 41) and control group (n = 42).The control group received conventional routine therapy, and treatment group was given rosuvastatin calcium 20 mg once per day on the basis of routine treatment. The levels of sera C-reactive protein (CRP), pulmonary function (FEV1、FEVl%pred), and COPD assessment test (CAT) were observed at the time points of 0 week and 12th week, 24th week and 48th week were compared between two groups. Results At 12th week after the treatment, sera CRP levels, FEV1, FEV1%pred and CAT scores in either control group or treatment group showed some extent of improvements as compared to those at the time point of 0 week, and there was statistical difference of sera CRP levels and CAT scores between two groups (P < 0.05). There was no statistical difference of FEV1, FEV1%pred between two groups (P > 0.05) .24th week and 48th week after the treatment; sera CRP levels, FEV, FEV1%pred and CAT scores in two groups all significantly improved and were statistical different between two groups (P < 0.05). Conclusion Statin can alleviate inflammatory reaction, improve pulmonary function and life quality of patients with COPD.
8.The value of 320 slice volume CT venography and ultrasound in diagnosis of lower extremity deep vein thrombosis
Junwei WANG ; Xinling CHENG ; Jianbo GAO ; Zhihui DONG ; Hua WANG ; Wei ZENG ; Peng GAO ; Zheheng MO
Journal of Practical Radiology 2016;32(10):1578-1581
Objective To study the value of 320 slice volume CT venography (CTV)and ultrasound in the diagnosis of lower limb deep vein thrombosis(DVT).Methods 51 patients with DVT confirmed by DSA were analyzed retrospectively,with comparing detection rate by direct method of CTV and ultrasound of the emboli in different parts of lower limb.Results In 5 1 patients,48 cases with DVTs were detected by CTV,including 124 thrombi,and 46 cases by ultrasound,finding 86 thrombi.CT diagnosed 34 pelvic deep vein thrombi,and ultrasound found 10.CT diagnosed 25 tibiofibular vein thrombi,and ultrasound found 5.CT diagnosed 2 femoral deep vein thrombi,and ultrasound found 1 1.Conclusion Direct method of CTV and ultrasound have high clinical value in the diagnosis of deep venous thrombosis,the former is better for the thrombosis in the pelvic deep veins and tibiofibular vein,while the latter is better for the thrombosis in the femoral deep vein.
9.The Clinic Study on the Treatment of Maxillary Protrusion Malocclusion with Micro-Screw Palatal Implant Anchorage
Xiaoying LI ; Cheng PENG ; Yi GUO ; Weie SUN ; Xiaoying CHEN ; Dong WANG
Tianjin Medical Journal 2014;(5):487-489
Objective To investigate the clinic application of micro-implant anchorage in the treatment of maxil-lary protrusion malocclusion. Methods Twenty-two patients,aged 18 to 25 years old,with maxillary protrusion were divid-ed into two groups:experimental group and control group with 11 patients in each group. All patients were treated with ex-traction. Micro-screw palatal implant was used in the cases of experimental group as orthodontic anchorage ,and traditional anchorage composed of extraoral arch used in the cases of control. The cephalometric films were measured before and after treatment. Statistical methods were utilized to analyze the morphological changes of facial profile and hard tissues in both groups. Results The values of U1-NA(mm:3.08±1.18 vs 8.15±3.05) and U1-SN(101.90°±3.50° vs 117.90°±6.05°) were sig-nificantly decreased after treatment compared with those before treatment in the experimental group ( P<0.01). The value of U1-L1(123.98°±5.78°vs 103.89°±8.95°) was significantly increased after treatment (P<0.01). In control group, the values of U1-NA (mm:5.01±1.34 vs 9.12±2.13) and U1-SN(101.90°±3.97° vs 114.87°±7.69°)were significantly decreased after treat-ment. The values of U1-L1(126.01°±3.12°vs 112.98°±5.98°) and U6-PtPNS(mm:21.45±2.43 vs 18.36±2.19)were significant-ly increased after treatment (P<0.05). The value of U1-L1(19.48°±8.90° vs 13.01°±5.90°) was significantly changed in exper-imental group than that of control group, but the value of U6-PtPNS(mm:0.90±0.29 vs 3.78±0.12)was significantly changed in control than that of experimental group (P<0.01). Conclusion The maxillary protrusion malocclusion with micro-im-plant anchorage can be used as treatment for patients with maxillary protrusion that needs strong anchorage.
10.Acute Toxicity Study on Intragastric Administration of Different ProcessedRadix Aconiti Lateralis PraeparataProducts to Beagle Dogs
Yuqin SONG ; Xue ZHANG ; Yanhong DONG ; Liangping DAI ; Cheng PENG ; Xiaofang XIE
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(7):1432-1437
This article was aimed to study the acute toxicities on intragastric administration of differentRadix Aconiti Lateralis Praeparataprocessed products to Beagle dogs. A total of 16 healthy and qualified Beagle dogs were randomly divided into the blank group,Pao-Fu-Pian(PFP) group,Pao-Tian-Xiong(PTX) group andHei-Shun-Pian(HSP) group according to the body weight. The intragastric administration of 4 g crude herb per kg was given. Before medication, 1 h, 24 h, and 3, 7, 14 days after medication, the body weight, food consumption, rectal temperature, electrocardiogram, blood routine and blood biochemistry were measured. The results showed that after medication, all dogs in three experimental groups were depressed. And there were significant differences in the electrolytes of blood. Among them, the HSP group was the most obvious one. The red blood cells, blood sugar and triglycerides of dogs in the PFP group had significant difference. The lymphocytes and blood sugar had significant difference of dogs in the PTX group. However, after the medication of HSP, the lymphocytes of the dogs were decreased significantly. It was concluded that the toxicity of three processed products followed the order of HSP > PFP > PTX.