Objective To evaluate the protective effect of large dose of ambroxol hydrochloride on lung ischemia reperfusion injury in rats and analyze the relationship between ambroxol hydrochloride and pulmonary vascular permeability mediators including EphrinAl, HIF-1α and VEGF at mRNA and protein levels.Methods Rats were operated by the thoracic posterolateral approach.The left lung is-chemia was induced by clamping the hilum of the left inflated lung for 60 minutes, and then followed by a 6-hour reperfusion.Forty rats were divided into ischemia-reperfusion group, ambroxol hydrochloride inter-vention group, sham operation group and blank control group.At the beginning of reperfusion, rats in am-broxol hydrochloride group were infused with ambroxol hydrochloride solution (3.75 mg ·kg~(-1) ·h~(-1)) via femoral vein for six hours.Then, PaO_2, wet-to-dry lung weight ratio, concentrations of IL-1β and TNF-a, activity of myeloperoxidase (MPO), mRNA and protein expressions of EphrinAl, HIF-1a and VECF were detected.Histopathological changes were observed under light microscope.Results (1) Histo-logical observation with light microscope showed significant decrease of diffuse alveolar damage consisting of septal edema, intraalveolar hemorrhage and exsudation in ambroxol hydrochloride group compared with ischemia-reperfusion group.The wet-to-dry lung weight ratio in ambroxol hydrochloride group was de-creased and PaO_2 was elevated significantly.(2) Compared with ischemia-reperfusion group, the concen-trations of IL-1β and TNF-α and activity of MPO in ambroxol hydrochloride group were decreased signifi-cantly.(3) The mRNA and protein expressions of EphrinAl, HIF-la and VEGF in ambroxol hydrochl-oride group did not recover to normal but were down-regulated significantly compared with ischemia-reper-fusion group.Conclusions (1) Large dose of ambroxol hydrochloride can alleviate the LJRI damage by down-regulating the expressions of IL-1β and TNF-α and the activity of MPO in the lung parenchyma.(2) The mRNA and protein expressions of EphrinA1, HIF-1α and VECF can be down-regulated by large dose of ambroxol hydrochloride, which contributes to alleviation of the LJRI damage.The results indicate possible role of gene regulation and fresh pharmacological effect of ambroxol hydrochloride during forma-tion of LIRI.

Objective To analyze the present research situation of stroke rehabilitation nursing in China, find out the defects and shortcomings existing in the development, and lay a good foundation for the development of stroke rehabilitation nursing. Methods The documentation published in Chinese core journals was searched in Chinese National Knowledge Infrastructure (CNKI), VIP database (VIP), Wanfang database by computer retrieval. The publishing year, journal name, scientific research funds, the method of literature research, research objects and research contents were analyzed by bibliometrics. Results A total of 253 articles were included with an increasing trend. These literature distributed in 34 kinds of Chinese core journals, fund rate was relatively good, but very few national fund, the experimental research was given priority to, and the research content was wide. Conclusions In order to better promote the rehabilitation of brain stroke patients, community rehabilitation care needs to be vigorously promoted, emphasis should be paid to the importance of patients family members, at the same time, great importance should also be attached to the combination between disciplines, in order to find better, more effective and easy methods to promote the rehabilitation nursing.

Objective To design and prepare recombinant mutant human high mobility group box 1 protein (HMGB1s) that can combine with HMGB1 receptors but cannot produce inflammatory effect, and accordingly lead to the creation of a new potential agent for anti-inflammatory therapy. Methods This experiment was based on successful clone and expression of human HMGB1.Six mutant HMGB1 cDNA were designed and constructed by one step inverse PCR. They were cloned into prokaryotic expressive vector pQE80L and followed with production of mutant HMGB1s and identification by Western blotting. Results Six mutant proteins were designed and constructed into prokaryotic expressive vector pQE80L. The recombinant HMGB1 proteins were obtained and identified by Western blotting. Conclusion Human HMGB1 mutants have been successfully constructed and the expression and characterization of intent proteins are identified. It will lay a foundation for further study on biological functions of HMGB1.

BACKGROUND:Bone marrow mesenchymal stem cel s have been shown to be differentiated into periodontal ligament fibroblasts when co-cultured with periodontal ligament cel s. Existing studies have shown that estrogen has the ability to influence bone marrow regeneration. OBJECTIVE:To investigate the effects of estrogen on osteogenesis and fibroblast-related factors alkaline phosphatase, type I and III col agen in bone marrow mesenchymal stem cel s. METHODS:Bone marrow mesenchymal stem cel s isolated from Beagle dogs were treated with estrogen. Osteogenesis and fibroblast-related mRNA and protein expression of bone marrow mesenchymal stem cel s was determined by RT-PCR and western blot assay, respectively. RESULTS AND CONCLUSION:mRNA and protein expression of type I and III col agen in bone marrow mesenchymal stem cel s was upregulated fol owing estrogen treatment;especial y, in contrast with type III col agen, the changes of type I col agen were more obvious. Estrogen did not influence mRNA and protein expression of alkaline phosphatase. These findings suggest that estrogen promotes the differentiation of bone marrow mesenchymal stem cel s into fibroblasts, whereas does not impact the genes involved in parodontium mineralization.

PurposeTo explore the value of transesophageal echocardiography (TEE) monitoring mini-incision transthoracic occlusion of ventricular septal defect (VSD) with asymmetric ventricular defects occlude in preoperative selection of patients, intraoperative guidance monitoring and postoperative evaluation.Materials and MethodsForty-five cases of VSD undertook mini-incision transthoracic occlusion with asymmetric ventricular defects occlude were enrolled, their clinical data was analyzed retrospectively, preoperative transthoracic echocardiography (TTE) was applied for choosing appropriate cases. During surgery, TEE was employed for evaluating the VSD and in which perimembranous VSD was found in 21 cases, intracristal VSD in 15 cases and subpulmonic VSD in 9 cases, suitable eccentric type occluders were chosen, guide occluder was placed, and the occlusion effect was evaluated right after operation.ResultsThe procedures were completed successfully in 42 cases, with a successful rate of 93.3%, of which 21 cases had perimembranous VSD, 15 cases had intracristal VSD, and 6 cases had subpulmonic VSD. The diameter of the VSD ranged from 3 to 7 mm, averaging (4.5±0.7) mm, the diameter of occluders ranged from 4 to 8 mm, averaging (5.7±1.2) mm, there was a good positive correlation between size of VSD and occlude (r=0.87,P<0.05). All patients received follow-ups from 3 months to 24 months after operation, all the occluders located normally, with no more than mildly residual shunt, valve regurgitation or severe arrhythmia discovered.ConclusionMini-incision transthoracic occlusion of ventricular septal defect (VSD) with asymmetric ventricular defects occlude has high success rates, minimal injury, and lower complication rate. TEE can play a vital role by improving the success rate and safety of surgery.

Objective The aim of this study was to assess the current situation and master degree on medical statistics among the medical correlation personnel, in order to provide useful information for transformation of education towards the medical correlation personnel . Methods Using cluster random sampling method to select 358 the medical correlation personnel who were recruited from health institutions in Chongqing to respond to the questionnaires. Questionnaire including respondent basic information, statis-tical knowledge and statistical knowledge needs. The data of the survey were analyzed by chi-square test, descriptive statistics analysis. Results 94.7% of the surveyed used statistical knowledge in the process of work or study. The main concentration of medical statistical knowledge utilization rate, the proportion of receive professional education or training courses of medical statistics and the willingness to participate in specialized training of medical statistics is 20-30 age group, No Title group, Master and over group andStudents group. As for ten categories of common statistical methods, thirty percent of people can use them skillfully, but the majority of the more advanced statistical analysis methods cannot be used or have never heard of. The greatest demand for learning ten kinds of common statistical methods is calculating cor-rectly through statistical software. Conclusion The vast majority of medical personnel need to use medical statistics in the actual work and study, but they have a lower mastery of statistical methods. Therefore, we should strengthen the medical statistics teaching and training for medical personnel.

To investigate the invasive ability of the residual tumor cells after immunotherapy and explore the feasible approach suppressing the invasion, mice were inoculated with B16 cells, and then treated by gene therapy with p4-1BBL/psPD-1 or IFN-gamma. The production and activities of MMP-9 and MMP-2 in residual tumor tissues were analyzed with gelatin zymography 1 day and 7 days after the termination of the immunotherapy. The production of MMP-9 and MMP-2 by B16 cells treated with IFN-gamma was also analyzed. IFN-gamma-treated B16 cells were inoculated to mice via subcutaneous injection. The invasion of tumor to muscular tissue was analyzed. Gene therapy with CH50 was used to suppress the invasive growth of tumor. The results showed that the expression and the activities of MMP-9 and MMP-2 were significantly increased 7 days after the end of immunotherapy. The response of tumor cells to ECM molecules was intensified after the removal of IFN-gamma, resulting in significant increase of both the production and activities of MMP-9 and MMP-2, and the increased invasion of tumor. Gene therapy with CH50 effectively suppressed the invasive growth of tumor. It is concluded that the termination of immunotherapy may result in a higher metastatic potential of residual tumor cells. Suppressing tumor invasion by suitable treatment will improve the efficacy of immunotherapy.

Integrity education is not only one of an important aspect of moral education topics for Medical postgraduates,but also an objective requirement in the socialist market economy.Aiming at the modern medical postgraduates'lack of credibility,this paper mainly analyzes its causes such as the limitation of postgraduates'quality and the pressure of employment and dis-cusses how to strengthen integrity education for postgraduate in medical university by bringing supervisors'leading role,enhance the standardized management of employment and upbuilding credibility file etc.

Objective To study the effect of nursing intervention on postural hypotension of aged with heart disease.Methods Nursing intervention were gived to the 68 aged patients with heart disease and postural hypotension.The effect of nursing intervention on postural hyporension and hypotension associated symptoms were observed.Results The postural hypotension was improved and the incidence of hypotension associated symptoms were reduced after nursing intervention.Differences were statistically significant(P＜0.01).Conclusion Nursing intervention was effective on the improvement of postural hypotension.

To study the influence of the compatibility of ophiopogonis tuber and Chinese magnoliavine fruit with gastrodia rhizome on the pharmacokinetics of gastrodin in rat, three dosages of compound Tianma granule extract (equivalent to gastrodin 50, 100, 200 mg x kg(-1)) and one dosage of Tianma extract (equivalent to gastrodin 100 mg x kg(-1) were administered to rats by intragastric administration separately. Plasma samples were collected at different times and treated with methanol and acetonitrile to precipitate protein. The contents of gastrodin in plasma were determined by HPLC method. The mean plasma concentration-time curves of different medication administration teams were processed with WinNonlin 5.2.1 pharmacokinetic software. The pharmacokinetic parameters of different medication administration teams were analyzed with SPSS statistics 17.0 software. The results indicated that the in vivo kinetic process of gastrodin was fitted to first-order absorption un-compartment model at low, middle dosages and zero-order absorption un-compartment model at high dosage of compound Tianma granule extract. By comparison with the pharmacokinetic parameters of gastrodin (100 mg x kg(-1)) in Tianma extract, the significant decrease for Cmax and significant increase for MRT0-infinity in compound Tianma granule extract indicated that the compatibility of ophiopogonis tuber and Chinese magnoliavine fruit with Gastrodia rhizome can delay the absorption, reduce the elimination rate and prolong the action time of gastrodin in vivo.