Objective To observe the effects of Tongyang Huoxue Recipe on cytoskeleton proteinβ-tubulin of ischemia reperfusion injured sinoatrial node cell in rabbits;To discuss its mechanisms in the treatment of sick sinus syndrome. Methods Sinoatrial node cells were obtained from newborn rabbit. Oxygen and glucose were deprived to simulate ischemia and were restored to simulate reperfusion. Cells were divided into 5 groups. Tongyang Huoxue Recipe high-, medium-, low dose groups were given corresponding medicine (final concentrations of 100, 20, 10 μg/mL). The normal group and model group were given equal volume of culture medium. Enzyme mark instrument and laser scanning confocal microscopy were used to observe the sinoatrial node cell activity and cytoskeleton protein β-tubulin of each group. Results Living cells of model group decreased significantly compared with normal group (P<0.01), and cytoskeleton proteinβ-tubulin cleavage significantly. Living cells of Tongyang Huoxue Recipe high, medium and low dose group were significantly higher than those in the model group (P<0.05), and β-tubulin structure remained more complete than the model group. Conclusion Tongyang Huoxue Recipe can inhibit the injury induced by ischemia reperfusion of sinoatrial node cells;the possible mechanism of Tongyang Huoxue Recipe in treating sick sinus syndrome is protecting activity and cytoskeleton proteinβ-tubulin of sinoatrial node cells.

OBJECTIVETo investigate the effects of prostate stromal cells from different zones of normal prostate tissue on the growth of prostate cancer cells and their action mechanisms.

METHODSWe extracted stromal cells in the fresh normal prostatic tissue derived from the peripheral zone (PZ) or transitional zone (TZ), amplified them in vitro, and used the supernatants of the cells as conditioned media to culture hormone-resistant prostate cancer DU145 cells. We measured the growth curve of the tumor cells using the CCK8 method, determined the number and viability of the cells by trypan blue staining, evaluated their invasiveness by scratch test, and detected the effects of the stromal cells on the key enzymes in the glycolysis of the tumor cells by Western blot.

RESULTSThe conditioned medium with the PZ-derived stromal cells promoted, while that with the TZ-derived stromal cells inhibited the growth of the tumor cells. The former significantly increased, while the latter markedly decreased the expressions of the key enzymes hexokinase 2 (HK-2), pyruvate kinase 2 (PKM-2), lactate dehydrogenase (LDHA), and pyruvate dehydrogenase (PDH) in the glycolysis of the tumor cells.

CONCLUSIONProstate stromal cells from different zones exert different influences on the growth of tumor cells, which may be associated with their different effects on the glycolysis of tumor cells.

Blotting, Western ; Cell Culture Techniques ; Cell Proliferation ; Culture Media, Conditioned ; Glycolysis ; Humans ; Male ; Prostate ; cytology ; Prostatic Neoplasms ; pathology ; Stromal Cells ; physiology ; Tumor Cells, Cultured

Adult ; Age of Onset ; Amino Acid Metabolism, Inborn Errors ; complications ; diagnosis ; genetics ; therapy ; Betaine ; administration & dosage ; therapeutic use ; Carrier Proteins ; genetics ; metabolism ; Child ; China ; epidemiology ; DNA Mutational Analysis ; Gas Chromatography-Mass Spectrometry ; Genotype ; Homocysteine ; urine ; Humans ; Hydroxocobalamin ; administration & dosage ; therapeutic use ; Hyperhomocysteinemia ; complications ; diagnosis ; genetics ; therapy ; Infant ; Methylmalonic Acid ; blood ; urine ; Mutation ; Vitamin B 12 ; metabolism

Objective To investigate the effect of oral lidocaine plasmagel on the postoperative odynophagia in patients receiving trans-oesophageal echocardiography (TEE) examination for cardiac surgery.Methods Sixty patients for cardiac surgery were divided randomly into the control group (C group) and the oral lidocaine plasmagel group (L group) with 30 cases in each group.Patients in L group received 2％ oral lidocaine plasmagel 20 ml before anesthesia,while C group did not.The incidence of postoperative odynophagia was compared between two groups.Results Postoperative odynophagia pain score were significantly higher in C Group than that in L Group.Conclusions Oral lidocaine plasmagel can alleviate the postoperative odynophagia in patients receiving trans-oesophageal echocardiography examination for cardiac surgery.

Objective To investigate effects of ginkgo biloba extract(GBE) and tanshinoneⅡ A preventative administration against cerebral ischemia and reperfusion injury in gerbils.Methods Cerebral iscthemia and reperfu- sion injury were made by 10 minutes'occlusion of bilateral carotid arteries followed by 24 hours' reperfusion in ger- bils.GBE,tanshinone Ⅱ A,nimodipine or GBE plus tanshinone Ⅱ A were administrated intragastrically 3 days prior to and at the day of ischemia and reperfusion.Effects of experimental agents on mortality,stroke index,brain body index,water content in brain hemisphere,level of SOD activity and MDA in cerebral tissue were measured,and pathological changes of cortex and hippoearnpal CA1 sector were observed.Results GBE 48 mg/kg and tanshinone Ⅱ A 25mg/kg preventive administration could significantly reduce the stroke index,brain body index,and water in brain hemisphere,together with a reduced mortality of cerebral ischemia and reperfusion injuried gerbils.The two a- gents could also significantly improve the activity of SOD,reduce the level of MDA in cerebral tissue and the injury in cortex and hippocampal CA1 sector.Moreover,combined treatment of these two agents demonstrated more signifi- cant effects.Conclusion GBE,tanshinone Ⅱ A and combined therapy of these two agents may protect cerebral func- tion from ischemia and reperfusion injury through reducing the cerebral edema and attenuating the injury of oxygen free radicals.

Objective To observe clinical efficacy of Banxiaxiexin tonga for the treatment of helicobacter pylori related peptic ulcers.Methods 110 patients were randomly divided into two groups.60 cases received Banxiaxiexin tonga for the treatment.50 cases in the control group received omeprazole triple therapy.Results The improvement in symptoms and signs,negative ulcer healing and Hp have made a good effect in treatment group.The total efficiency is 91.6% ,the total effective rate in control group is 78.0%.There was significant difference between the two groups(P <0.01).Treatment group had no adverse reaction,the control group was 24.0% ,the difference between the two groups was significant(P < 0.01).There was no significant difference between the two groups in hpclearance(P >0.05).Conclusion Banxiaxiexin tonga had good effect on the treatment of Hp-related peptic ulcer.

Objective This paper has discussed the antibacterial、 antiviral and degrading poison property of TiO2 nanometer spray in air cleaning. Methods Viable count was carried out, the inactivation on hepatitis B antigen (HbsAg) and the degradation of formaldehyde were detected. Results The results demonstrated that the inactivation to Staphylococcus aureus, Escherichia coli were over 99%. Bacillus subtilis var. niger, Candida albicans and hepatitis B antigen(HbsAg) were inactivated to a certain extent. The spray may be used for degrading the formaldehyde in the indoor air. Conclusions These two kinds of TiO 2 nanometer spray may, to a certain extent, inactivate the pathogenic bacteria and HbsAg, and may degrade formaldehyde.

The paper analyses statistically the parameters of acceleration and heart rates in 905 aerobatic maneuvers of 88 sorties. The result indicates that the G levels, rates of increas as well as the duration of acceleration in tactical flight training are higher and longer than routine flight training. There is a significant correlation between the pilot's heart rates and the magnitude of G levels. The pilot's endurance for operating aircraft actively was 1.4G higher in average than that of the centrifuge runs. Af-ter anti-G physiological training, the endurance can be increased 2.24G in average by means of using anti-G suit and L-1 maneuver. The endurance was increased by an average of 1.18G as compare withthat prior to physiological training.

Acupuncture Therapy ; Enteritis ; diagnosis ; therapy ; Eosinophilia ; diagnosis ; therapy ; Gastritis ; diagnosis ; therapy ; Humans ; Male ; Middle Aged

ObjectiveTo observe the effects of catalpol, the effective component of Rehmanniae Radix, on atrionector cell apoptosis andβ-tubulin in vitro rabbit injured by simulated ischemia reperfusion;To explore the mechanism of treating sick sinus syndrome.MethodsAtrionector cells were collected from newborn rabbits. Cells were divided into 5 groups:normal group, model group, catalpol high, medium and low dose groups. Anoxia and aglycaemia were established to simulate ischemia. Atrionector cellular damage models were established by recovering the supply of oxygen and sugar. Normal control group and model group were given the same volume of culture medium, while catalpol high, medium and low dose groups were given medicine with relevant concentrations (100, 20, 10μg/mL, respectively). ELISA, FCM, laser scanning confocal microscopy were used to observe apoptosis rate andβ-tubulin of atrionector in each group.Results Apoptosis rate of the model group was obviously higher than the normal group (P<0.01), andβ-tubulin cleavage was obvious. Apoptosis rate in catalpol high, medium and low dose groups were significantly lower than that of the model group (P<0.01);β-tubulin structure were significantly more complete compared with the model group;the fluorescence intensity was significantly higher than that of model group (P<0.01).Conclusion Catalpol can inhibit atrionector cellular apoptosis caused by simulated ischemia reperfusion. Its protective effects on atrionectorβ-tubulin may be the mechanism of the treatment of Rehmanniae Radix for sick sinus syndrome.