The characteristics of medical and health literature novel assessment in institutions of armed forces were described from the secrecy of projects for literature novelty assessment , the limitations of consignee institution , and the non-profitability of literature novelty assessment agency .The major problems of medical and health literature novel assessment in institutions of armed forces were elaborated in terms of the literacy, instability, competency and knowledge limitation of literature novel assessment staff with suggestions put forward for their solution such as strengthening the construction of talent team, joint development and sharing of resources.

BACKGROUND:Ilizarov technique is an effective treatment method which can solve various complications that occurred in the process of treatment, especial y for the treatment of nonunion associated with soft tissue defects or severe infection. OBJECTIVE:To review the research progress of nonunion treatment with the Ilizarov technique. METHODS:A computer-based search was performed on the CNKI database and PubMed database from January 1992 to April 2013 for the articles related to the Ilizarov technology for the treatment of nonunion. The key words of“nonunion, external fixator, Ilizarov”in English and“nonunion, fracture nonunion, external fixation, Ilizarov”in Chinese were used to search the articles in the title and the abstract. Final y, a total of 37 articles were included to review. RESULTS AND CONCLUSION:Ilizarov technique is an effective method to treat limbs nonunion with the infection and severe bone defect. It has become an international y recognized gold standard. Due to the firmly fixation of the Ilizarov ring external fixator, it can not only eliminate the shear force and rotation stress, thus playing a role of stretch tension and compression, but also has the cyclical micro-axialmotion characteristic during weight-bearing walking which can better promote bone union. Based on the reviews of the research progress of Ilizarov technique in the treatment of nonunion in recent years, we further investigate the advantages and methods of Ilizarov technique in the treatment of limbs nonunion. The clinical application of the method should fol ow the individual principle, and then choose the best and suitable method based on the analysis of advantages and disadvantages about the patient’s condition.

Objective:Through the analysis of the 64 rows of 128 slice spiral CT two years usage data, establishes CVP analysis model.Methods: Using the rate of return on investment, the investment recovery period, cost recovery rate, the amount of profit, data analysis method, etc.Results: Comprehensive embodiment of equipment through various analysis methods of operation efficiency, to use department and management department to provide relevant data, and provide reference for the rational use of equipment.Conclusion: Through the large medical equipment benefit analysis, can make the hospital more clear understanding of medical equipment to hospitals with economic benefits, so as to improve the efficiency in the use of equipment, reduce the waste of money.

Objective: To measure the expression of E cadherin (E cad) and ? catenin (CTN) in transitional cell carcinomas (TCC) of bladder and to study the relationship between the expression and biological behavior of the carcinoma. Methods:Expression of E cad and ? CTN were measured by flow cytometry in 30 cases of frozen bladder carcinomas. Results: The fluorescence index (FI) of E cad and ? CTN of tumor cells were 0.5677?0.3194 and 0.5726?0.2895, respectively,which were significantly lower than those of normal bladder mucosa ( P

Objective:To investigate the effect of EPO on humoral and cellular immunity and the monocyte antigen presenting function in patients with chronic renal failure(CRF).Methods:60 patients with CRF were divided into three groups.Group A patients accepted hemodialysis and treated with EPO.Group B patients treated with EPO and didn't accept hemodialysis.Group C patients didn't accept EPO treatment and hemodialysis.After 8 weeks treatment,the subpopulations of T lymphocyte were analyzed with detected by flow cytometry analysis method;Immunoglobulins were measured with radioactive immuntzation method.In addition,the infection rate of patients with CRF in every group was compared.Results:The parameters of immune functions of patients with CRF were comparable between groups before treatment.After treated with EPO for 8 weeks,RBC numbers and Hb were significantly increased in patients of group A and group B,but not in patients of group C.IgG and IgA were increased in patients of groups A and groups B and have statistic differences as compared with that in patients of group C(P

Objective:To compare the effect of fluorescence staining and periodic acid-Schiff staining in the diagnosis of fungal keratitis (FK).Methods:A total of 147 corneal specimens from 147 FK patients treated in Eye Hospital of Shandong First Medical University from January 2017 to May 2019 with positive corneal scraping or fungal culture were collected.Among them, there were 84 cases with penetrating keratoplasty (PKP), 42 cases with lamellar keratoplasty (LKP) and 21 cases with lesion resection.Another 11 cases with herpes simplex virus keratitis served as negative control.The corneal tissue specimens were performed with fungal fluorescence staining and periodic acid-Schiff staining, respectively.The stained sections were placed under fluorescence microscope and optical microscope to observe fungal hyphae or spores, respectively.The positive rates of the two staining methods were compared, and the positive cases of the diagnosis of FK in corneal tissue samples obtained by different surgical methods and corneal infection caused by different strains of the two staining methods were compared.Written informed consent was obtained from each patient.The study protocol adhered to the Declaration of Helsinki and was approved by the Ethics Committee of Shandong Eye Hospital (No.SDSYKYY-2016012).Results:The positive rate of periodic acid-Schiff staining and fungal fluorescence staining was 60.5% (89/147) and 79.6% (117/147), respectively.The positive rate of fluorescence staining in the diagnosis of fungal keratitis was significantly higher than that of periodic acid-Schiff staining ( χ2=28.00, P<0.01), and both the specificity of the two staining methods was 100%.The positive rate of specimens obtained by PKP with fluorescent staining was 85.7% (72/84), and the positive rate with periodic acid-Schiff staining was 65.5% (55/84), and the difference was statistically significant ( χ2=17.00, P<0.01). The positive rate of specimens obtained by LKP with fluorescent staining was 71.4% (30/42), and the positive rate with periodic acid-Schiff staining was 52.4% (22/42), and the difference was statistically significant ( χ2=8.00, P<0.01). The positive rate of resected foci specimens with fluorescent staining was 71.4% (15/21), and the positive rate with periodic acid-Schiff staining was 57.1% (12/21), and the difference was not statistically significant ( χ2=1.30, P=0.25). The positive cases of two kinds of staining were different among different fungal strains.Among them, the positive cases of Fusarium solani complex, Pythium insidiosum, Aspergillus fumigatus complex, Candida guilliermondii, Trichoderma and Nigrograna mackinnonii with fluorescence staining were 19, 5, 5, 1, 1 and 1, and the positive cases of periodic acid-Schiff staining were 11, 0, 3, 0, 0 and 0, respectively.The staining results of the 11 negative controls were negative. Conclusions:Fluorescence staining is more sensitive than periodic acid-Schiff staining in the detection of fungal components in paraffin-embedded corneal tissues, and it can significantly improve the fungal detection rates.

Traditional Chinese medicine has a long history of intranasal administration. Compared with the other administration routes, intranasal administration has the benefits of fast absorption, high bioavailability, high brain-targeting and non-invasive. In the past few years we take "Xingnaojing" and "Tongqiao Sanyu formula" as model drug and studied pharmacokinetics of effective components of different polarities. MDCK/MDCK-MDR1 cells were used to simulate blood brain barrier to study the permeate behaviors of different drug and the mechanism of enhancing effects of aromatic medicine. Then a microemulsion (modified by mPEG2000-PLA) was prepared for intranasal administration, and the pharmacokinetics and investigated tissue distribution were studied by fluorescence imaging. The irritation of the drug and different preparations were studied on human nasal epithelial cell (HNEC) cell and living animals. In this paper, we reviewed the achievements and hope that it can provide constructive suggestions for the future research.
Administration, Intranasal ; instrumentation ; methods ; Animals ; Biological Availability ; Blood-Brain Barrier ; drug effects ; Drugs, Chinese Herbal ; administration & dosage ; pharmacokinetics ; Humans

Objective To investigate the effects of the site for access to internal jugular vein (lateral versus anterior),lying position of patients (supine versus Trendelenburg),and head rotation (0°,20°,and maximum) during central venous catheterization on the location depth and cross-sectional area (CSA) of the right internal jugular vein (IJV).Methods Fifteen healthy volunteers were recruited in this prospective observational study from September 2008 to October 2008.Healthy volunteers were placed in flat supine position and 15°.Trendelenburg position separately.In each position,IJV were measured ultrasonographically from lateral site and anterior site with the head oriented at 0°,20°,and maximum rotation separately.Data of measured CSA and location depth of internal jugular vein in different positions were compared.Results The largest CSA (2.16 ±0.89) cm2 and location depth [(1.38 ± 0.43)cm] were occurred at the lateral approach in Trendelenburg position with head oriented at maximum rotation.The CSA in Trendelenburg position was larger than that in flat supine position.Only at the maximum head rotation,lateral approach got statistically larger CSA.The effects of head rotation varied with different degrees of rotation.Conclusions Site of approach,lying position and head rotation had noticeable effects on internal jugular vein cross-sectional area.Trendelenburg position increased the CSA of IJV.

ObjectiveTo establish a novel rapid detection method based on PCR-single-strand-conformational polymorphism (PCR-SSCP) to determine mutation of streptomycin-resistance associated rpsL and rrs genes in isolates of Mycobacterium tuberculosis (MTB).MethodsStreptomycin-resistance of 112 MTB isolates was detected using the routine drug susceptibility test,and a special PCR-SSCP assay was established.The mutations of rpsL and rrs genes in streptomycin-resistant MTB isolates were detected by PCR-SSCP and PCR direct sequencing (PCR-DS) ; the results from two techniques were compared.Results All isolates had both rpsL and rrs genes.Fifty-two isolates (46.4％) were streptomycin susceptible,in which only 1 isolate showed abnormal PCR-SSCP fragments from rrs gene,and the specificity of PCR-SSCP was 98.1％ (51/52).Sixty isolates (53.6％) were streptomycin-resistant,in which 46 (76.6％) and 11 ( 18.3％ ) isolates presented the abnormal PCR-SSCP fragments of rpsL and rrs gene,respectively.One streptomycin-resistant isolate showed abnormal PCR-SSCP fragments from both rpsL and rrs genes.The sensitivity of PCR-SSCP was 93.3％ (56/60).ConclusionThe PCR-SSCP that established in this study is a specific and sensitive method for rapid detection of the streptomycin-resistance associated mutations in rpsL and rrs genes of MTB.

In order to explore the clinical effect of the glass fiber-reinforced composite resin post core for severe defect restoration of front teeth,sixty-two teeth were restored for forty-one patients with severe defect restoration of front teeth by glass fiber-reinforced composite resin post core plus alumina all-ceramic crown.Through 0.6-2.0 years follow up,all the prosthesis worked very well without breaking or falling off,no gum coloring except two posts fell off after the restoration at sixth month.The patients satisfied with the restoration.