Objective To study the expression of connective tissue growth factor(CTGF) and PEST containing nuclear pro‐tein(PCNP) and their significance in pancreatic cancer .Methods The expressions of CTGF and PCNP proteins were tested by im‐munohistochemistry and Immunofluorescence in 39 cases of pancreatic carcinomas and adjacent paracancerous tissues .Results The positive rate of CTGF and PCNP in pancreatic carcinomas was significantly higher than adjacent paracancerous tissues(χ2 =60 .41 , 51 .46 ,all P<0 .01) .The differences of the expression of CTGF and PCNP in pancreatic carcinoma of tumor differentiation ,TNM stage and lymph node metastasis was significant (P< 0 .05) .Conclusion The expression of CTGF and PCNP in the pancreatic cancer was obviously increased ,and the level of CTGF ,PCNP had remarkable connection with the stages of tumor and the condition of lymph node metastasis .

Objective To investigate the effects of sodium arsenite (NaAsO2) on cell survival circumstance,reactive oxygen species (ROS) and cell apoptosis in human normal hepatic cells (L-02).Methods L-02 cells were exposed to different doses of NaAsO2 (0,50,100,150 μmol/L) for 24 h.MTT assay was used to detect the survival of L-02 cells,and flow cytometry (FCM) was used to detect the ROS levels and the early (Q4),late (Q2) apoptosis of L-02 cells.Results Cell survival rate:cell survival rate was compared between groups,the difference was statistically significant (F =350.51,P ＜ 0.05),the cell survival rates of 50,100 and 150 μmol/L NaAsO2 groups [(87.30 ± 3.74)％,(49.03 ± 4.72)％,(13.44 ± 4.01)％] were significantly lower than that of the control group [(100.00 ± 0.00)％,all P ＜ 0.05];compared with 50 μmol/L NaAsO2 group,the cell survival rates of 100 and 150 μmol/L NaAsO2 groups were significantly decreased (all P ＜ 0.05);compared with 100 μmol/L NaAsO2 group,the cell survival rate of 150 μmol/L NaAsO2 group was significantly decreased (P ＜ 0.05).The ROS levels:ROS levels were compared between groups,the difference was statistically significant (F =407.78,P ＜ 0.05),the ROS levels of 100 and 150 μ mol/L NaAsO2 groups (3 212.00 ± 221.93,5 521.33 ± 179.63) were significantly higher than that of the control group (1 691.67 ± 73.98,all P＜ 0.05);compared with 50 μmol/L NaAsO2 group (1 927.67 ± 62.45),the ROS levels of 100 and 150 μmol/L NaAsO2 groups were significantly increased (all P ＜ 0.05);compared with 100 μmol/L NaAsO2 group,the ROS level of 150 μ mol/L NaAsO2 group was significantly increased (P ＜ 0.05).Cell apoptosis:cell apoptosis rates of Q2,Q4 and Q2 + Q4 were compared between groups,the differences were statistically significant (F =256.84,26.53,63.89,all P ＜ 0.05);excecpt the cell apoptosis rate of Q4 in 50 μ mol/L NaAsO2 group [(5.43 ± 0.57) ％],the cell apoptosis rates of Q2 [(5.67 ± 0.21)％] and Q2 + Q4 [(11.10 ± 0.40) ％] in 50 μ mol/L NaAsO2 group,the cell apoptosis rates of Q2 [(13.60 ± 0.79) ％],Q4 [(7.37 ± 2.01) ％] and Q2 + Q4 [(20.97 ± 2.38) ％] in 100 μmol/L NaAsO2 group,the cell apoptosis rate of Q2 [(13.47 ± 0.78) ％],Q4 [(16.97 ± 3.45) ％] and Q2 + Q4 [(30.43 ± 3.84) ％] in 150 μmol/L NaAsO2 group were significantly higher than those of the control group [Q2:(3.47 ± 0.12) ％,Q4:(2.90 ± 0.90) ％,Q2 + Q4:(6.37 ± 1.00) ％,all P ＜ 0.05];compared with 50 μmol/L NaAsO2 group,the cell apoptosis rates of Q2,Q4 and Q2 + Q4 in 100 and 150 μmol/L NaAsO2 groups were increased,except the cell apoptosis rate of Q4 in 100 μ mol/L NaAsO2 group,the differences were statistically significant (all P＜0.05);the cell apoptosis rates of Q4 and Q2 + Q4 in 150 μmol/L NaAsO2 group compared with 100 μmol/L NaAsO2 group were significantly increased (all P ＜ 0.05).Conclusions NaAsO2 can induce L-02 cells to increase ROS levels,and inhibit L-02 cell proliferation.In addition,NaAsO2 can induce early apoptosis and late apoptosis in L-02 cells.

Objective To assess the effectiveness of transpupillary thermotherapy (TTT) for the treatment of central exudative chorioretinopathy. Methods Tweenty nine eyes with central exudative chorioretinopathy were treated with Iris 810 nm diode laser TTT. The laser beam size was 1.0, 2.0 or 3 0 mm with power settings between 80 300 mW and treatment time 60 sec. The follow up periods were wihzin 4 40 weeks. The therapeutic effect was accessed by visual acuity examination,dinect ophthalmoscopy and fluorescein or indocyanine green angiography. Results The visual acuity improved in 8 eyes (28%), remained no change in 19 eyes (65%) and decreased in 2 eyes (7%). Choroidal neovascularization were closed in 12 eyes in fundus angiography. The symptoms alleviated in 10 patients. Conclusion Transpupillary thermotherapy is a potential treatment for the central exudative chorioretinopathy.

Adult ; Crown Lengthening ; Dental Prosthesis ; Dental Prosthesis Design ; Esthetics, Dental ; Female ; Gingiva ; Humans ; Incisor ; Smiling

Objective To investigate the expression of folate receptor(FR) in primary hepatocellular carci noma and to explore its correlation with clinical and pathological features.Methods Immunohistochemistry was used to detect the expression of FR alpha and beta in primary hepatocellular carcinoma (45 cases),cirrhosis (32 cases) and normal liver tissue (20 cases).And then the relationship between the expression of FR alpha and clinic0pathological features was analyzed.Results The positive expression of FR alpha was detected in 88.9％ (40/45) primary hepatocellular carcinoma,18.8％ (6/32) cirrhosis,and there was no positive expression of FR alpha in normal liver tissue.There was no FR beta expression in primary hepatocellular carcinoma and cirrhosis,only 15％ (3/20) normal liver tissue expressed FR beta.The expression of FR alpha did not correlate with gender and age,but the positive expression of early-mid-term patients was higher than that of the advanced patients.Conclusion FR alpha is presented in most liver tissues of primary hepatocellular carcinoma,which provide basis of early diagnosis and molecular drug target.

Objective To understand AIDS related knowledge and behaviors among female sex workers(FSWs) and their male clients from the same community.Methods One hundred and seventy seven FSWs and one hundred and fifty four male clients were recruited from entertainment venues in the same community by random sampling,and anonymous questionnaires were administered separately.Results(1)Over 80% of FSWs and their male clients understood the basic routes of HIV transmission,but many of them were unclear about non-HIV transmission routes.(2)97.7% of FSWs and 84.2% of the male clients used condoms during their commericial sexual intercourse.The frequency of using condoms during sex with casual or regular sex partners was lower than that during the commercial sexual intercourse(P

Objective To assess the virtual non-contrast liver CT from dual-energy CT for the clinical application. Methods In total, 51 patients were included in the study, and all patients underwent multi-phase liver CT on a dual-source CT. The True non-contrast liver CT (TNCT) was performed in a single-energy acquisition mode, but the arterial and portovenous liver CT (VNCT) were performed in a dual-energy mode of 110 kV and 140 kV respectively. The virtual non-contrast CT images were derived from the arterial data using liver virtual non-contrast software. Between the true non-contrast CT and the virtual non-contrast CT, the image quality, mean CT HU values in the liver and muscle, signal to noise (SNR), the radiation dose of volume CT dose index (CTDIvol) and dose length product (DLP) in a single phase and total examination were compared with t test. Results There was no significant difference in the detection of liver lesions between TNCT and VNCT. The CT Hu values of muscle on both TNCT and VNCT images were almost equal. The CT HU values of liver on VNCT images were higher than that on TNCT images and the difference was significant [61.32 ±6. 04 vs. (56. 85 ±4. 80) HU, t = -3. 927,P＜0.01]. There was also significant differenc of SNR between TNCT (11.28±2. 78) and VNCT (8.65 ± 1.56) images( t =-5.590,P＜0.01). The CTDIvol and DLP of single phase were (7.07 ±0.85) mGy and (155.11 ±respectively, but in TNCT the total CTDIvol and DLP reached (21.43 ± 2. 46 ) mGy and (469. 02 ±significance, but the total CTDIvol and DLP were significantly different (t = 16. 168 and 13. 132, P ＜0. 01). Conclusion With the consequent reduction in radiation dose, the VNCT can replace TNCT as an imaging protocol in multi-phase abdominal CT examination in clinic.

Arrhythmias, Cardiac ; etiology ; physiopathology ; Humans ; Myocardial Infarction ; physiopathology ; Sympathetic Nervous System ; physiopathology