Macrophage migration inhibitory factor (MIF) is a cytokine of pluripotent biological behavior. With the in-depth research of MIF on molecular biology and clinic, it has been found that MIF is closely re-lated to the gastrointestinal tumor' s proliferation and invasion. In this review, we mainly discuss the correla-tion between MIF and gastrointestinal tumor.

目的探讨针刺配合促进技术对脑梗死患者上肢功能及日常生活活动能力(ADL)的影响。方法将114例脑梗死上肢瘫患者随机分为针刺配合促进技术组(A组)、针刺组(B组)、促进技术组(C组)和对照组(D组),应用针刺疗法和促进技术康复治疗。观察治疗前后简式Fugl-Meyer评分(FMA),Barthel指数 (MBl)和简易上肢功能评分的改变。 结果治疗后A组明显优于其他3组,B组和C组无差异,A、B、C各组治疗后优于D组。结论早期针刺和促进技术联合治疗是急性脑梗死上肢瘫早期康复治疗较为理想的方法。

Objective To investigate the expression of p53 in human ovarian neoplasms by tissue array technique Methods The expression of p53 protein in various ovarian tissues was studied by tissue array and immunohistochemistry Results The expression rate of p53 was 33% in ovarian cancers There were no expressions in normal ovarian tissues, benign ovarian neoplasms and borderline ovarian neoplasms( P 0 05) while p53 expression was associated with tissue types( P

The paper reviewed the medical service pricing at home and abroad and progress in the price comparison system,and introduced the price comparison system of Shanghai's medical services. Proposals in the paper include building the price parameters fitting local conditions,and setting prices using the standardized price model;forming a comprehensive price adjustment scheme with reference to international price comparison relations and domestic pricing level; upgrading the fine pricing management level of medical service pricing and improving charging management dimensions;enhancing interactions and assessment of price-related departments to guide public hospitals to enhance.

Objective:To investigate the changes of intestinal flora and serum amino acid levels in elderly patients with Parkinson's disease(PD)at different stages, and to provide evidence for diagnosis, prevention and treatment of PD.Methods:A total of 140 elderly PD patients diagnosed and treated in our hospital from January 2018 to August 2019 were enrolled and divided into PD course <5 years group(n=70)and PD course≥5 years group(n=70). Ninety subjects without PD confirmed by medical examination in our hospital were included as the control group.Real-time fluorescence quantitative PCR of 16S rDNA gene was used to detect levels of Bifidobacterium, Lactobacillus, Clostridium prasus, Enterococcus, Enterobacter, Prevotella copri and Akkermansia muciniphila bacteria in the gut, and the high-performance liquid chromatography was used to detect the serum amino acids in the three groups.Results:Compared with the control group, the levels of Bifidobacterium, Lactobacillus, Clostridium prasalis and Prevotella copri in the intestines were decreased and Enterobacter level was increased in PD course <5 years group and PD course≥5 year group( F=20.863, 32.251, 23.166, 24.683 and 10.136, all P<0.001). The serum levels of methionine, tryptophan, lysine and glutamate in PD course <5 years and PD course≥5 year group were decreased as compared with the control group( F=5.858, 5.877, 4.183 and 25.462, all P<0.05). Spearman correlation analysis showed that serum glutamate level was positively correlated with Clostridium prastia in the intestine( r=0.647, P=0.002)and negatively correlated with Prevotella copri( r=-0.559, P=0.010). Multivariate Logistic regression analysis showed that the protective factors in the intestines of elderly PD patients were Bifidobacterium( OR=0.186, 95% CI: 0.054-0.637, P=0.007), Lactobacillus( OR=0.283, 95% CI: 0.098-0.816, P=0.020), Clostridium prastia( OR=0.232, 95% CI: 0.063-0.851, P=0.028), and Prevotella copri( OR=0.222, 95% CI: 0.058-0.851, P=0.028), and the risk factor was Enterobacter( OR=5.119, 95% CI: 1.406-18.636, P=0.013). Conclusions:The decreases of Bifidobacterium, Lactobacillus, Clostridium, Prevotella copri and Enterobacter levels in the intestinal tract of PD patients, and the decrease of serum glutamate level may be correlated to the progression of PD.

AIM:To observe the expression of Toll like receptor 4 (TLR4) in the left ventricle of Goldblatt rats and to explore the role and mechanism of TLR4 in left ventricular remodeling of hypertension.METHODS:Goldblatt model of Two-kidney,one-clip (2K1 C) renovascular hypertension was induced in twenty-five rats(H group),and twenty rats served the sham-operated group(sham group).The tail cuff blood pressure was detected every week and echocardiogram was observed every other week.After eight weeks of operation,the rats were killed and the samples of the left ventricle were collected.The concentration of Ang Ⅱ in left ventricle was assessed by tadioimmunoassay.Western blotting and RT-PCR were used to exam the mRNA and protein expression of TLR4 in the left ventricle.Immunohistochemistry was adopted to exam the location of TLR4 in the myocardium.RESULTS:TLR4 mRNA and protein expression were consistently upregulated in the left ventricle of H group compared with sham group.In H group, predominantly sarcolemmal staining was observed,especially focal areas of intense TLR4 staining were found in juxtaposed regions of two or more adjacent myocytes;However,in sham group,TLR4 expression was diffuse and presumably cytoplasmic.Considerable correlation was found between blood pressure,MESS,LVMI,RWT,the concentration of Ang Ⅱ in left ventricle and the protein expression of TLR4 in myoeytes.CONCLUSION:During the development of left ventricular remodeling of Goldblatt rats,expression of TLR4 increases significantly.Enhanced expression of TLR4 locates in the sarcolemma,especially in juxtaposed regions of two or more adjacent myocytes.These indicate that TLR4 transmembrane receptor which is closely relative with inflammation and immunity probably contributes to the development of ventricular remodeling.[KEY WORDS]Hypertension;Ventricular remodeling;Receptors,Toll-like;Goldblatt rats

Objective To study the clinical characteristics of primary Sj(o)gren's syndrome (pSS) patients with dry eyes.Methods Ninety-six pSS patients with dry eyes admitted to the Department of Rheumatology and Immunology,the Affiliated Hospital of Guilin Medical College from August 2009 to August 2011 were retrospectively analyzed.Results In all 96 patients,89 patients had symptoms,23 of whom had two or more symptoms.The tear-film break-up time (BUT) of 154 eyes were Less than 5 seconds,18 were between 5-10 second,and twenty were more than 10 seconds,with the average of 3.7±1.9 seconds.The length of tear flow of 154 eyes'width of tear mocus was less than 3 millimetres (mm),with the average of (0.21±0.10) mm.The average score of corneal fluorescein staining score was 5.2±2.4.The wet lengths of filter paper of 192 eyes were all less than 10 mm/5 min,with the average of (4.6±1.8) mm.The average score of meibomian gland function was 1.5±0.9.Conclusion In patients with pSS,the width of tear meniscus and the wet lengths of filter paper are reduced,which implies less tear secretion.At the same time,BUTs are short,and the Meibomian gland dysfunction are observed.Dry eyes of pSS are mixed in type,not only aqueous tear deficiency.

Objective To investigate the effects of repeated intraperitoneal dexmedetomidine on the cognitive function in rats with chronic cerebral ischemia.Methods Forty-eight male Sprague-Dawley rats,aged 3-4months,weighing 250-300 g,were randomly divided into 4 groups (n =12 each) ∶ sham operation group (group S),chronic cerebral ischemia group (group IS),dexmedetomidine treatment 1 group (group DXM1) and dexmedetomidine treatment 2 group (group DXM2).Dexmedetomidine 5 μg/kg was injected intraperitoneally at 30 min before occlusion of bilateral common carotid arteries and 3,12,24 and 48 h after occlusion in group DXM1,and at 3,12,24 and 48 h after occlusion in group DXM2.The cognitive function was assessed by Morris water maze 2 weeks after occlusion.The apoptosis was examined by TUNEL.The expression of Bcl-2 protein in hippocampus was detected by Western blot.Results Compared with group S,the escape latency was significantly prolonged from 2nd day to 5th day after the place navigation test in group IS and on 2nd day after Morris water maze test in groups DXM1 and DXM2,and the time of staying in 1 st quadrant was significantly shortened,the apoptotic rate was increased,and the expression of Bcl-2 was up-regulated in groups IS,DXM1 and DXM2 (P ＜ 0.05).Compared with group IS,the escape latency was significantly shortened from 3rd day to 5th day after the place navigation

Against the deficiencies of the current preventive medicine education curriculum,training programs and curriculum were reformed according to the training objectives of the modem preventive medicine, while new ideas of preventive medical personnel training were explored in order to cultivate practical professionals with a certain ability to innovate and to adapt the development of modern public health and preventive medicine preferably.

Objective To evaluate the prognostic value of the neuron-specific enolase ( NSE ) and bispectral index ( BIS) in patient with mild therapeutic hypothermia ( MTH) after cardiopulmonary resuscitation ( CPR ) .Methods Forty-six patients with restoration of spontaneous circulation ( ROSC ) after CPR were treated with MTH.The BIS values were recorded and the serum NSE was measured at the following times:24 h, 48 h, and 72 hours after ICU admission.Neurological outcome was classified according to the Pittsburgh cerebral performance category ( CPC 1 to 5) at 3 months after ICU discharge.Results Fourteen patients had a good neurological outcome with CPC score 1-3, and thirty-two patients had a poor neurological outcome with CPC 4-5 at 3 month.Compared with good outcome group, the NSE values were significantly higher in the poor outcome group on day 2 and day 3 after admission [48 h: (90.1 ±42.7) ng/mL vs.(33.2 ±17.5) ng/mL;72 h: (95.4 ±37.0) ng/mL vs. (29.2 ±17.0) ng/mL, P<0.05].NSE increased markedly in the poor neurological outcome group at 48h and 72h, and decreased significantly in the good group at the same time [△NSE 24 h-48 h: (37.3 ±28.7) ng/mL vs.(-10.7 ±12.1) ng/mL; △NSE 48 h-72h: (5.3 ±13.2) ng/mL vs.(-4.0 ±4.5 ng/mL), P<0.05].Over the 72 h of monitoring, the mean BIS values were lower in the poor outcome group compared to the good outcome group at 48 h [ (39.2 ±24.1) vs.(78.0 ±12.4); 72 h: (45.7 ±26.4) vs.(89.0 ±7.3), P<0.05].Conclusions The values of NSE and BIS were effective prognostic indicators for the neurological outcome of patients with MTH after CPR.