OBJECTIVE:To study the safety of percutaneous administration of Hongzhi gutong cataplasm. METHODS:Rab-bits were taken for single dose in complete skin irritation test(n=4),single dose in damaged skin irritation test(n=4)and multi-ple doses in complete skin irritation test(n=4). The left and right sides of the skin respectively paste 3 cm×3 cm blank matrix and Hongzhi gutong cataplasm for 24 h(calculated by crude drug of 0.14 g). After removing tape 1,24,48,72 h,the erythema and edema in hair removal site of rabbits in the former 2 tests were observed;after 24 h of administration,the rabbits in the last group were administrated again after exposing the administration area for 1 h,repeated 3 times,the erythema and edema in hair removal site after removing tape the first,second time and 1,24,48,72 h in the third time were respectively observed. RESULTS:In the 3 experiments,the scores of erythema and edema of all rabbits were 0,and skin irritation was evaluated as no irritation. CONCLU-SIONS:Hongzhi gutong cataplasm has no skin irritation in rabbits.

Agenesis of Corpus Callosum ; Chromosomes, Human, X ; Gene Deletion ; Glycerol Kinase ; genetics ; Humans ; Infant ; Male ; Syndrome

Backgroud EDTA is an important calcium chelator,commonly used as an ophthalmic additive,its role in promoting corneal penetration of drug may facilitate transepithelial corneal collagen crosslinking.However,there were few reports about the penetration of riboflavin to corneal stroma enhanced by EDTA both home and abroad.Objective To determine the concentrations of riboflavin in corneal intrastromal under different interventions using high-performance liquid chromatography (HPLC).To ensure the efficacy and safety of 0.5％ EDTA in riboflavin penetrating to corneal stroma by the transepithelial procedures.Methods Fifteen New Zealand white rabbits were randomized into three groups,corneal epithelium of 5 rabbits were debrided,corneal epithelium of another 5 rabbits were left in situ and 0.5％ EDTA-Na2 was topically applied for 1 hour,and the corneal epithelium of last 5 rabbits were just left in situ,after those procedures 0.1％ riboflavin + 20％ dextran was applied topically to the corneas for 30 minutes(1 drop every 3 minutes).Then,the epithelium in the corneas of EDTA and epi-situ group were carefully removed before all the corneas were punched with an 8.5 mm diameter blade and homogenized subsequently.Ultimately,the riboflavin concentrations were determined by HPLC.Results After treated with riboflavin for 30minutes,the mean concentration of riboflavin in the epi-removed group was (23.54± 1.61)μg/g tissue,the EDTA group was (2.04 ±0.25)μg/g tissue and the epi-situ group was (1.44 ±0.06)μg/g tissue,showing significant difference among them (F =1792.839,P =0.000).There were statistically significant differences between any two groups (t =41.780,7.484,43.408,all at P ＜ 0.05).However,the riboflavin concentration of other two groups were failed to meet the theoretical value 15 μg/g tissue,excepted for epi-removed group.Conclusions The diffusion of riboflavin to the corneal stroma can be fully enhanced by removing the epithelium preoperatively,penetration enhancer EDTA can effectively promote the penetration of riboflavin to the cornea,but it still can not replace the role of removing epithelium on the penetration of riboflavin to the cornea.

Female ; Humans ; Infant ; Mitochondrial Proton-Translocating ATPases ; deficiency ; genetics ; Mutation

Objective To investigate the feasibility, safety and short-term efficacy of endoscopic implantation of 5-FU Slow-release Particles for advanced gastroenteric tumor. Methods During the endoscopy procedure,slow-releasing 5-FU agents were implanted densely into the tumors and infiltrated area. Forty-five to sixty pieces of agents ( each piece equivalent to 1.67 mg 5-FU)were injected, which containing an average dose of 100 mg 5-FU. Results A total of 13 advanced gastric cancer patients were enrolled into this study. Significant effects were observed in 3 patients and good effects in 8 patients, but 2 cases with no effects. The total effective rate was 84.62%. The endoscopy examination showed that the size of tumors reduced in various degree at 1 - 2months after the implantation. No hemorrhage or perforation was observed. Parameters of hepatorenal function and routine blood test were stable after implantation. Conclusion Endoscopic implantation of 5-FU Slow-release Particles can relieve the symptoms of patients and limit tumor growth in advanced gastroenteric tumor with no marrow and hepatorenal functional impair.

Hydrophidae, one of the precious traditional Chinese medicines, is generally drily preserved to prevent corruption, but it is hard to identify the species of Hydrophidae through the appearance because of the change due to the drying process. The identification through analysis on gene barcode, a new technique in species identification, can avoid the problem. The gene barcodes of the 6 species of Hydrophidae like Lapemis hardwickii were aquired through DNA extraction and gene sequencing. These barcodes were then in sequence alignment and test the identification efficency by BLAST. Our results revealed that the barcode sequences performed high identification efficiency, and had obvious difference between intra- and inter-species. These all indicated that Cyt b DNA barcoding can confirm the Hydrophidae identification.
Animals ; Base Sequence ; China ; Cytochromes b ; genetics ; DNA Barcoding, Taxonomic ; Elapidae ; classification ; genetics ; Molecular Sequence Data ; Phylogeny ; Reptilian Proteins ; genetics ; Sequence Analysis, DNA

Humans ; Infant ; Male ; Mandibulofacial Dysostosis

Objective To observe enhancing effect of nerve regeneration on peripheral nerve defect models bridged by silicone tube idled with valproic acid (VPA). Methods In present research we demon-strate the effect of VPA on peripheral nerve regeneration and recovery of motor function following sciatic nerve transaction in rats. An 8-mm sciatic nerve deficit was created in a rat mode land bridged by a 1-cm silicone tube.Then, 10 lad of 8% VPA were perfused into the silicone chamber in the VPA group. The same volume of normal saline was delivered in the control group. Results Each animal was observed sciatic nerve function index (SFI) at 2-week intervals and studied electrophysiology at 4-week intervals for 12 weeks. Histological and morphometrical analyses were performed at the end of the experiment, 12 weeks after operation. Using the digital image-analysis system, thickness of the myelin sheath was measured, and total numbers of regenerated axons were counted. There was a significant difference in SFI, electrophysiological index (motor-nerve conduct velocity, MCV), and morphometrical results (regenerated axon number and thickness of myelin sheath) in nerve regeneration between the VPA group and controls (P ＜ 0.05). Conclusion The results demonstrated that VPA is able to enhance sciatic nerve regeneration in rats, suggesting the potential clinical application of VPA for the treatment of peripheral nerve injury in humans.

OBJECTIVE:To discuss the effect of early usage with Wulingjun powder on the behavior of rats with post-traumat-ic stress disorder(PTSD)and the level of interleukin-1β(IL-1β)and IL-6 in their hippocampi. METHODS:50 SD rats were ran-domized into control group(normal saline),model group(normal saline),positive control group(paroxetine,10 mg/kg)and the groups of low and high-dose(0.4,0.8 g/kg)Wulingjun powder. The rats in all groups except for control group,were exposed to single prolonged stress for the establishment of PTSD models. Corresponding drugs were given ig immediately after such establish-ment,qd,for 7 consecutive days. An open field test(the times of the rats’entering the central area and the percentage of the time the rats spent in exploring the central area within 10 min were recorded)was conducted 7 days after giving drugs;and an elevated plus-maze test(the times of the rats’in opening arms and the percentage of the time the rats spent in opening arms within 5 min were recorded)was carried out 8 days thereafter,to observe the behavior of the rats. Then the rats were sacrificed to determine the level of IL-1β and IL-6 in their hippocampi by ELISA. RESULTS:Compared to the normal group,the behavioral index of the rats in model group was significantly lower,and the level of IL-1β and IL-6 in their hippocampi was significantly higher(P<0.05 or P<0.01). Compared to model group,the behavioral index of the rats in positive control group and the group of high-dose Wuling-jun powder was significantly higher,and the level of IL-1β and IL-6 in their hippocampi was significantly lower (P<0.05),but no statistically significant difference was shown in all indexes in the group of low-dose Wulingjun powder (P>0.05). CONCLU-SIONS:High-dose Wulingjun power can improve the anxiety-like behavior of the rats with PTSD,which may be related to the re-duction in the level of IL-1βand IL-6 in the hippocampus.

Objective To explore the clinical characteristics and prognostic factors of breast carcinosarcoma.Methods Clinical data of 37 breast carcinosarcoma patients treated at Tianjin Cancer Hospital from 1974 to 2014 was analyzed retrospectively.Kaplan-Meier unvariate analysis was used to calculate the survival rate and compare the survival rates.Multivariate factors for survival were analyzed by COX proportional hazards regression model.Results 37 cases of breast carcinosarcoma were all female.The median age was 55 years.Among them 31 cases complained for painless mass,six cases had breast mass with skin ulceration.21 cases were three-negative breast cancer.Ten cases had axillary lymph node metastasis,ten cases had distant metastasis.The 5-year survival rate was 62.3％.Unvariate analysis showed that axillary lymphnodes metastasis (P =0.045) and therapeutic schedule (P =0.016) significantly influenced the oulcome of the patients.COX multivariate analysis validated that the therapy modality was an independent prognostic factor for breast carcinosarcoma(P =0.041).Conclusions Breast carcinosarcoma is rare and has a poor prognosis.ER,PR and HER-2 expressions in most cases are negative.If there is axillary lymph node metastasis,the prognosis is poor.A reasonable and comprehensive treatment can improve the prognosis.