1.Oligonucleotide Array Comparative Genomic Hybridization and Its Application
Yi-Fei PENG ; Wen-Li MA ;
China Biotechnology 2006;0(10):-
The array CGH technique (Array Comparative Genome Hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. It is mainly used in human genetics and oncology. Generally PCR amplified bacterial artificial chromosomes (BACs) or cDNAs have been spotted on the arrays as probes. Recently, however, oligonucleotide arrays designed with more flexibility and provide much higher resolution with high sensitivity, have been successfully explored in stead of BAC array CGH and can save considerable time and efforts. There will be a gradual transition from BAC array CGH to oligonucleotide array CGH in the coming years. The combination of oaCGH and other high-through put analysis can lead to discoveries of a host of novel oncogenes, tumor suppressors as well as tumor drug resistance genes. Some major platforms of oaCGH concerning their spatial resolution, optimal probe length, sensitivity, specificity and application in recent years were compared.
2.The expression of ACE, AT1, ACE2, MAS on heart from WKY and SHR.
Peng-Fei LI ; Wei ZHANG ; Chang MA ; Yuan-Shu ZHANG
Chinese Journal of Applied Physiology 2011;27(2):153-224
Animals
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Hypertension
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metabolism
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physiopathology
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Male
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Myocardium
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metabolism
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Peptidyl-Dipeptidase A
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genetics
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metabolism
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Proto-Oncogene Proteins
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genetics
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metabolism
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RNA, Messenger
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genetics
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metabolism
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Rats
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Rats, Inbred SHR
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Rats, Inbred WKY
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Receptor, Angiotensin, Type 1
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genetics
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metabolism
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Receptors, G-Protein-Coupled
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genetics
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metabolism
4.Preparation characterization and antitumor activity in vitro of berberine hydrochloride polymeric micelles.
Wen-zhuan MA ; Jin-ling WANG ; Peng-fei TU
China Journal of Chinese Materia Medica 2015;40(21):4182-4188
With polyethylene glycol vitamin E succinate (TPGS) as the carrier materials, and berberine hydrochloride ( BER) as model drug, we formed berberine hydrochloride (BER) -loaded TPGS nanomicells (BER-PMs) using filming-rehydration method to improve its solubility and in vitro anti-tumor effect. The transmission electron microscope (TEM) was used to observe the particle appearance; particle detector was used to detect the diameter and Zeta potential; and ultracentrifugation was utilized to determine the encapsulation efficiency (EE) and drug-loading (DD); dynamic dialysis method was used to study the in vitro release behavior of BER-PMs, and the anti-tumor activity against MCF-7 cells was determined by MTT method. Results showed that the average particle size of BER-PMs was (12.45 ± 1.46) nm; particle size was uniform and spherical; drug loading and encapsulation efficiency were (5.7 ± 0.22)% and (95.67 ± 5.35)%, respectively. Zeta potential was (-1.12 ± 0.23) mV; release rate within 24 h was 37.20% and 41.14% respectively in pH 7.4 and pH 6.5 phosphate buffer in vitro; compared with BER, BER-PMs can significantly inhibit MCF-7 cell proliferation (P < 0.05), promote cell apoptosis and improve the anti-tumor activity of BER in vitro. Therefore, the formed berberine hydrochloride micelle can more effectively promote the apoptosis of MCF-7 cell, and improve the drug's in vitro anti-tumor effect.
Antineoplastic Agents
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chemistry
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pharmacology
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Berberine
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chemistry
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pharmacology
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Cell Death
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drug effects
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Cell Survival
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drug effects
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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MCF-7 Cells
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Particle Size
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Polymers
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chemistry
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pharmacology
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Solubility
5.Isolation and purification of total tubeimosides from Bolbostemma paniculatum by macroporous resin.
Ting-jun MA ; Peng-fei TU ; Fei-jie LV
China Journal of Chinese Materia Medica 2005;30(10):744-746
OBJECTIVETo select the optimum purification process of all tubeimosides from Bolbostemma paniculatum by macroporous resin.
METHODStatic absorption test and deabsorption test were carried out to screen the best macroporous resin for all tubeimosides. The single factor test, orthogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macroporous resin.
RESULTThe macroporous resin NKA possessed the strongest absorption ability to all tubeimosides among the 9 resins studied, in addition to an easy de-absorption property. The optimum absorption conditions were A2B3C1, namely concentration of feed 30 mg x mL(-1), diameter: height of the chromatography column 1:7, the weight ratio of raw material and resins 1:2.
CONCLUSIONThe above experimental results can provide experimental basis for large-scale purification process of all tubeimosides.
Cucurbitaceae ; chemistry ; Plants, Medicinal ; chemistry ; Resins, Synthetic ; Rhizome ; chemistry ; Saponins ; isolation & purification ; Technology, Pharmaceutical ; methods ; Triterpenes ; isolation & purification
6.Purity Determination of NaNO_2 and Assessment of Uncertainty
Juan SHEN ; Yongxue LI ; Jiangang WU ; Fei MA ; Peng ZHAO ; Jiange JIA
Chinese Medical Equipment Journal 2003;0(10):-
Objective To determine the purity of sodium nitrite(NaNO2) using in stray light standard substance which was applied to check semiautomatic clinical chemistry analyzer.Methods Titration was used to determine the purity of NaNO2,the uncertainty of the purity was assessed,and a parallel test was used to validate the uncertainty.Results The purity of NaNO2 was 99.81%,and its uncertainty was 0.86%.Conclusion The uncertainty of the purity passes the validation,it shows that the purity test is credible,and its uncertainty assessment is logical.
7.Study on fine carbon fiber powder injected into cranium of rabbits
Kexiang FEI ; Xiangao PENG ; Yanxiang ZHANG ; Ling MA ; Jinwu WANG ; Lin GAO
Chinese Journal of Pathophysiology 2001;17(8):735-
AIM: To study dynamically the effect on the rabbits after fine carbon fiber powder was transplanted into their cranium. METHODS: After fine carbon fiber powder was injected into subdural cavity of rabbits, examination of histopathology and EEG were carried out. RESULTS: General observation: 1-104 weeks after injecting, no neuropathological changes concerning with the injecting of the carbon fiber powder were found in the rabbits of experimental group; Under optical microscopes: 1-2 weeks after injecting, slight inflammatory reaction in meninx was found and disappeared generally 4 weeks after injecting, no obvious fiber membrane was found around the carbon fiber, no significant differences were showed between the experimental group (EG) and the control group (CG); Examination of EEG: 1-2 weeks after injecting, slight abnormal changes of EEG in both two groups were showed, but no significant differences was found between them. 4 weeks after injecting, the EEG of the two groups was restored to their normal state before injecting. 1-8 weeks after injecting, no obvious epilepsy waves were showed. CONCLUSION: The fine carbon fiber powder showed excellent histocompatibility after injected into the subdural cavity of rabbits.
8.Histopathological study on fine carbon fiber powder injected into the subdural space of mice
Kexiang FEI ; Xiangao PENG ; Lin GAO ; Ling MA ; Mingcan WU ; Weiquan SUN
Chinese Journal of Pathophysiology 2001;17(8):733-
AIM and METHODS: After the fine carbon fiber powder was injected into the right subdural space of the mice, dynamic observation was carried out on their movement and histopathological changes. RESULTS: 1-52 weeks after the injecting, no neurological changes concerning with the implanting of the carbon fiber powder were found in the experimental mice. The fine carbon fiber extensively located on the inter surface of the dura mater membrane of the right temporalis and the out surface of pie mater. Only slight inflammatory cells reaction was found under optical microscopes. The degree of inflammation reaction are Grade Ⅱ 1 week after injection and was Grade Ⅰ 2 weeks after injection, inflammation was disappeared 4 weeks after injection. No obvious fiber membrane was found around the implanted materials. No significant differences were found between the experimental and the control group.CONCLUSION: It was showed that the carbon fiber shares excellent histocompatibility after injected into the subdural space and subarechnoid cavity of the right temple of mice.
9.PCDH19 gene mutations lead to epilepsy with mental retardation limited to females in 2 cases and literature review
Li YANG ; Arafat AHMED ; Jing PENG ; Chen CHEN ; Yuping MA ; Fei YIN
Journal of Central South University(Medical Sciences) 2017;42(6):730-736
Epilepsy with mental retardation limited to females (EFMR) is a syndrome characterized by early onset heat-sensitive epilepsy of infancy or early childhood and generally limited to females,which previously reported that the cadherin gene superfamily subtype protocadherin 19 (PCDH19)gene is its pathogenic gene.We retrospectively analyzed the clinical data for 2 cases of EFMR patients with PCDH19 mutation diagnosed by Department of Pediatric Neurology of Xiangya Hospital,Central South University in 2015.Literature on PubMed,OMIM and HGMD relevant to this syndrome was reviewed,and the clinical characteristics were summarized accordingly.The 2 cases are consistent with the typical clinical manifestations of EFMR caused by PCDH19 mutations.Their seizures are heat sensitive,with or without screaming,and expressed in various forms.Cognitive impairment or autism-like performance were often identified in these patients,hematuria metabolic diseases screening was normal,no abnormal MRI imaging of the head,and de novo PCDH19 gene mutations were found in their epilepsy gene chip sequencing.It is noteworthy that this disease is very similar to the clinical manifestations of the Dravet syndrome due to the mutations of the neurotype sodium channel αl subunit SCN1A.Therefore,in female patients whose clinical manifestations resemble to Dravet syndrome but SCN1A gene test were negative,EFMR with PCDH19 mutation should be taken into consideration.Early PCDH19 gene testingis of great significance because it not only helps clinicians to understand and analyze the prognosis of this disease,but also offers genetic counseling to the parents.
10.Choice and Practice of the new transform moed of science and technology achievemevts of military medicine
Zhongwu LIN ; Yi PENG ; Bin TAN ; Shinan CAO ; Junjie MA ; Fei DU ; Zhongze WANG
Chinese Journal of Medical Science Research Management 2014;27(5):541-543,553
With the development of civil military integration,military scientific research institu tes are facing the challenge of constructing a new mode of translating scientific and technological achievements into practice and enhancing translational efficiency.This paper began with the evolution of translation mode in military institutes and discussed the flaws and insufficiency of current mode,then a triple helix translation mode,which encompass government,industry and research,was introduced and fully explained for future reference.