Objective To investigate the protective effects of Cilazapri l on focal cerebral ischemia of spontaneous hypertensive rats. Method Focal cerebral ischemia of spontaneous hypertensive rats wer e made by occlusion of the middle cerebral artery(MCA). Cilazapril treated group was taken cilazapril 4 weeks before model was made. TTC staining,HE staining,Nis sl's staining,immunohistochemistry, and image analysis were taken to estimate th e area of ischemia,number of dead neural cells,optical density of positive cell of Nissl's staining,HSP70 and NF-k BP65 immunohistochemistry reaction in ischem ia area. Result Cilazapril could reduce the area of ischemia and behavior sc ores,decrease the number of died neural ce lls,increase the optical density of positive cell of Nissl's staining,reduce th e optical density of NF-k BP65. No effect on HSP70 immunohistochemistry reactio n was found. Conclusion Cilazapril could decrease infarction of focal ischemia o f spontaneous hypertensive rats, promote the functional restoration of rats,decr ease nerve cell death,inhibit the expression of NF-k B. Cilazapril inhibited in flammatory reaction and that could be one of its protective mechanisms.

Objective:To explore the multiple meditation effects of emotional intelligence and self-esteem between interpersonal initiative personality and social positive adaption in left-behind junior high school students.Methods:Totally 331 left-behind junior high school students [165 boys,160 girls,average age of (14.5 ± 1.2) years] were selected.They were assessed with the Interpersonal Self-Support Scale for Middle School Students (ISSS-MSS),Emotional Intelligence Questionnaire (EIQ),Rosenberg Self-Esteem Scale (RSES) and Social Adjustment Scale (SAS-YB).Results:The scores of social positive adaption were positively correlated with the scores of interpersonal initiative personality,EIQ and RSES (r ＝ 0.47-0.67,Ps ＜ 0.001).Path analysis showed that interpersonal initiative personality had direct effects on social positive adaption (β ＝ 0.47,P ＜0.001).Mediation modeling showed that interpersonal initiative personality could exert effects on social positive adaption indirectly through the mediating effect of not only emotional intelligence,but also self-esteem (x2/df＝ 3.03,TLI ＝ 0.87,CFI ＝ 0.89,RMSEA ＝ 0.08).Conclusion:The present study indicates that parallel multiple mediation model of emotional intelligence and self-esteem could explain the mechanism of influence of interpersonal initiative personality on social positive adaption appropriately.

0.05).However,other groups based on different extent and degree of pathology,clinical risk had significant difference in hs-CRP(P

A database model of ontology was established by mining the EMR-covered data according to the con-cept of fuzzy vector space model with Kawasaki disease as an example. The evidence and model for the diagnosis and treatment of Kawasaki disease were detected fromthe EMR of Kawasaki disease in the database model of ontolo-gy in order to help the clinicians to make its differential diagnosis and improve its treatment plan, thus achieving the effective EMR data mining and promoting the computer-aided clinical decision-making.

On the basis of assuming that the postgraduate education charge is a fact,this paper at fist studies the theory and realistic basis of postgraduate student charges and then analyzes its profit and fraud. Finally,some constructive suggestions are put forward to protect the enrollment chances of poor students and the development of basic discipline.

Objective: To get the information about freshmen's mental health and it relationship with sex, city and countryside difference, and to provide the reference for effective mental health education and consultation. Methods: The situation of 1351 freshmen's mental health was surveyed by using UPI. Results: ①11.91% of the freshmen had serious mental derangement, 26.15% of them had a little psychological problem. ②The distinct difference existed not only between male and female, but also between city and countryside. The mental health of male students surpassed the female students, the city students surpassed the countryside students. Conclusion: The situation of medical freshmen's mental health should not be neglected, and its influence factors are various. Mental health education and consultation should be pointed to the students.

Biodegradable nano/microparticles of poly(D, L-lactide-co-glycolide) (PLGA) is a novel non-viral gene vector, which has many advantages, such as safety, non-immunogenicity, easy of large-scale preparation and well load-capability. Therefore, more and more attentions and researches have been paid on its application. Especially, PLGA has shown enormous potential application value and space in the field of plasmid DNA (pDNA) delivery system. On the basis of the current situation of PLGA nano/microparticles for pDNA delivery, this paper focused on summarizing the current preparation approaches and surface modified methods of PLGA particle, furthermore showing its application in gene therapy and genetic vaccine delivery. These showed that PLGA nano/microparticles have extensive prospect in the development of controlled gene delivery system.

Objective To study the expression of interferon regulatory factor 1 (IRF-1) in patients with systemic lupus erythematosus (SLE) and the influence of prolactin (PRL) upon it. Methods The level of serum PRL in quiescent condition was examined by electrochemiluminescence-meter in 30 patients with SLE and 20 healthy controls. Peripheral blood mononuclear cells (PBMC) were separated from all the subjects by gradient centrifugation density, and cultured with or without the presence of recombinant human PRL (rhPRL) for 24 hours. The expression of IRF-1 gene in cultured PBMC was detected by reverse transcriptase-PCR (RT-PCR) with gel image scanning. Results The relative value of IRF-1 gene expression was significantly higher in SLE patients than in normal controls (0.89±0.21 vs 0.78±0.18, P=0.026), and in SLE patients with high PRL than in those with normal PRL (1.06±0.26 vs 0.82±0.21, P=0.005). However, there was no significant difference between SLE patients with normal PRL and healthy controls in regard to the expression of IRF-1 gene (P=0.514). The stimulation with rhRPL significantly elevated the relative expression of IRF-1 gene in SLE patients with normal PRL (0.99±0.22 vs 0.82±0.21, P=0.036), but had no obvious effect on that in the normal controls. Conclusion The study reveals a high expression of IRF-1 gene in SLE patients, which may be related to the high level of PRL.

Objective To investigate HIV high risk population size estimation method,analyze the epidemic situation in China and offer scientific evidence for policy making. Methods Appropriate methods to estimate different type high risk populations were ascertained by experimental study. Results Size estimation methods and procedures for different type high risk population were formed.Epidemic estimation and parameters fit for China were ascertained.HIV epidemic situation and characteristics were stated. Conclusion Methods formed in the study are the key points for the understanding of number of PLWHA,for the exact judgment of HIV epidemic,for the improvement of inspection system and examination work,for the formation of "Four Free and One Care".

Objective To investigate the values of computed tomography(CT) and endoscopic retrograde cholangiopanc reatography(ERCP) in acute biliary tract obstruction associated with Clonorchiasis. Methods The image features of 47 patients with acute biliary tract obstruction associated with Clonorchiasis proved by operation (27 cases) and ERCP (20 cases) were reviewed retrospectively. Results Fourty-three cases showed equal intrahepatic bile duct diffuse dilatation from porta hepatis to capusle with different levels in CT, and 39 cases showed extrahepatic bile duct dilatation. There was no significant relationship for the extent of intra and extrahepatic bile duct dilatation. It showed the end of intrahepatic bile duct shaped like a bag in 41 cases, a enlarged gallbladder in 38 cases, and 9 cases with a soft intralumenal masses. There were 13 patients with pancreas swelling and 8 with pancreatic duct dilatation. Of the 20 patients underwent ERCP, eighteen cases showed intrahepatic bile duct diffuse dilatation with the end shaped like a bag, and 16 showed extrahepatic bile duct dilatation. Irregular filling-defect was seen in the common bile duct in 9 cases. The obstruction were relieved after Oddi's muscle endoscopic sphincrectomy(EST). Conclusion The patients with acute obstructive jaundice can be diagnosed as Clonorchiasis, if CT or ERCP showed equal intrahepatic bile duct diffuse dilatation with the end shaped like a bag and with extrahepatic bile duct dilatation, which can be confirmed by the worms and eggs found in the drawing-out bile, and the obstruction can be relived by EST.