Adolescent ; Adult ; Female ; Humans ; Male ; Niemann-Pick Diseases ; genetics ; Pedigree

Objective: To study the relationships among attributional style, self-efficacy and subjective well-being(SWB). Methods: 269 college students participated the study. Attributional Style Questionnaire , Self-efficacy Questionnaire and Subjective Well-being(SWB) Questionnaire were applied to the college students. Results: ① Attributional style predicated SWB, but three dimensions (internality-externality; whole-part; controllability-uncontrollability)of negative events were significant negative predicators of SWB. ② Self-efficacy was a significant positive predicator of SWB. ③ There were no differences in SWB between sexes and specialities. ④ There were significant differences in SWB between students with different self-efficacy levels, and the SWB of high self-efficacy group was better than that of the low self-efficacy group. Conclusion: College students'attributional style and self-efficacy impact their subjective well-being.Educators should cultivate students's positive attributional style and enhance their self-efficacy.

Objective To investigate the protective effect and mechanisms of NF-?B decoy oli- godeoxynucleotides(ODNs)on rat liver graft following ischemia-reperfusion injury.Methods Ani- mals were randomly divided into 3 groups(n=8 in each group):control group,ischemia-reperfusion (IR)group,and decoy ODNs group,in which donor grafts were transfected with 120?g NF-?B decoy ODNs before graft procurement.Following 2 h of reperfusion,Kupffer cells(KCs)were isolated,and NF-?B biding activity was detected with electrophoretic mobility shift assay(EMSA),and TNF-?、IL- 6 mRNA expression was assayed by using RT-PCR method.Meanwhile,the liver and serum samples were collected,the histopathologic change of liver was examined by light microscope,and liver func- tion was analyzed.Results The NF-?B biding activity,TNF-?,IL-6 mRNA expression,and the ALT,TBIL levels in serum were significantly increased following 2 h of reperfusion in IR group as compared with those in control group(P＜0.01).A large amount of degeneration and necrosis in hep- atocytes accompanied with obvious congestion in hepatic sinusoid occurred.However,these tested in- dexes were significantly ameliorated in decoy ODNs group as compared with those in IR group(P＜0.01),and the architecture of hepatic lobules was remained.Conclusions KCs NF-?B activation fol- lowing reperfusion plays an important role in IRI in liver transplantation.Decoy strategy shows appar- ent effect on suppression of NF-?B activation,and thus inhibits the production of downstream cyto- kines,which protects the liver graft from IRI.

Objective To investigate the effect of oral lidocaine plasmagel on the postoperative odynophagia in patients receiving trans-oesophageal echocardiography (TEE) examination for cardiac surgery.Methods Sixty patients for cardiac surgery were divided randomly into the control group (C group) and the oral lidocaine plasmagel group (L group) with 30 cases in each group.Patients in L group received 2％ oral lidocaine plasmagel 20 ml before anesthesia,while C group did not.The incidence of postoperative odynophagia was compared between two groups.Results Postoperative odynophagia pain score were significantly higher in C Group than that in L Group.Conclusions Oral lidocaine plasmagel can alleviate the postoperative odynophagia in patients receiving trans-oesophageal echocardiography examination for cardiac surgery.

BACKGROUND:The rise of tissue engineering has opened up new ways for tissue repair and reconstruction of the urinary tract, and the bladder acellular matrix is a better alternative material for urinary tissue engineering.
OBJECTIVE:To construct the compound of hair fol icle stem cells with heterologous bladder acellular scaffold, and to observe the growth of hair fol icle stem cells on the scaffold.
METHODS:Bladder acellular matrix from New Zealand rabbits were prepared and detected using scanning electron microscopy and Masson staining. Passage 3 hair fol icle stem cells were statical y inoculated into the surface of bladder acellular matrix using secondary sedimentation method. Under inverted microscope, cellgrowth was observed, and cellgrowth curves were drawn. cellgrowth on the scaffold surface was observed through histological detection and scanning electron microscope observation.
RESULTS AND CONCLUSION:Prepared bladder acellular matrix was a white translucent film with fiber mesh structure, and no residual cells were seen. Masson staining results indicated that the bladder acellular matrix had col agen structure, and no obvious residual cells. After culture for 48 hours, hair fol icle stem cells grew wel around the bladder acellular matrix under inverted microscope;1 week later, hair fol icle stem cells extended and adhered to the scaffold surface. These findings indicate that hair fol icle stem cells have a good biocompatibility with the bladder acellular matrix through in vitro culture.

[Objective]Electrophoretic analysis of rat chemically extracted acellular nerve allograft(CEAN) protein was performed to investigate the protein of CEAN.Dorsal root ganglia of chicken were cultured onto the surface of CEAN slice to observe the effect of CEAN structure on the fiber regeneration.[Method]The CEAN were perpared according to Sondell method and electrophoresed to observed whether the electrophoresis strip of 28-30kDa(Myelin Protein) exited or not.Dorsal root ganglia of chicken were cultured onto thc surface of CEAN slice and dyed with nerve fiber fluorescence to observe the orientation of nerve fiber growth on the surface of CEAN slice.[Result]Electrophoretic analysis of rat CEAN protein showed that the electrophoresis strip of 28-30 kDa totally disappeared.A large number of nerve fibers grew from DRG along the basement membrane of CEAN.[Conclusion]No myelin protein remained in the CEAN,while the basement membrane structure of CEAN can induce the nerve fiber to grow.

[Objective] To investigate the biological characteristics of Wharton’[KG-38x]s jelly-derived mesenchymal stem cells (WJMSCs) and their differentiation into Schwann cell-like cells, and to provide a new cell seed source for nerve tissue engineering or cell therapy. [Methods]Umbilical arteries, veins and umbilical cord tunica externa were removed , and the remaining tissue (Wharton’[KG-38x]s jelly) was harvested.The umbilical cord was cut into small fragments and cultured with DMEM in orderto gather. Cultured WJMSCs were confirmed by the detection of MSC-specific cell-surface markers. WJMSCs were differentiated along a glial cell lineage using an established cocktail of growth factors. Immunocytochemical staining and Western blot were used to evaluate the characteristics of differentiated WJMSCs.[Results]WJMSCs were immunonegative for the haemopoietic cell-surface markers (CD34 and CD45) and neural stem cell marker (nestin), and immunopositive for MSC surface markers CD44, CD105, Stro-1 and vimentin. WJMSCs treated with a mixture of glial growth factors adopted a spindle-like morphology similar to Schwann cells. Immunocytochemical staining and Western blot analysis revealed that the treated cells expressed the glial markers p75 and glial fibrillary acidic protein indicative of differentiation.[Conclusion]WJMSCs can be differentiated into the cells that are Schwann-like in terms of morphologic features and phenotype and may be suitable as Schwann-cell substitutes for nerve repair in clinical application.

Objective To evaluate the effect of 3-dimmentional conformal radiotherapy (3-DCRT) for mediastinal metastasis from esophageal carcinoma after surgery.Methods Sixty-three such patients were treated by 3-DCRT. Tumor diameter was: cm 20 patients. Positive supraclavicular lymph node was found in 9 patients. The number of tumor at the upper right, upper left and upper median mediastinum was 27, 27 and 20. All patients received 3-DCRT to the dose of 40?Gy (2?Gy/f, 5f/w), after resetting of portals, the dose was boost to 64～70?Gy by 3?Gy/f, 5f/w. Dose of the patients who had been irradiated was boosted to 50?Gy without further positioning. Results Complete response rate and partial response rate were 43% and 48% one month after the treatment was concluded,with no progression of disease. The 1-, 2- and 3-year survival rates were 75%, 43% and 15%. Conclusions Three-dimmentional conformal radiotherapy, with little side-effects, can markedly improve the local control rate and suvival rate of patients with mediastinal metastasis from esophageal carcinoma after surgery.

Objective To compare the differences between antrum and corpus in endoscopic and histological diagnosis in non-ulcer dyspeptic patients.Methods 132 cases from out-patient department who had been ordered to undergo gastroendoscopy for dyspepsia were enrolled in the study.After endoscopy,different signs including redness,erosion,intramural bleeding and rugal atrophy were described.Histological diagnosis was defined according to the classification of chronic inflammation,polymorphonuclear neutrophil activity,glandular atrophy,intestinal metaplasia,helicobacter pylori and dysplasia.The differences between antrum and corpus in endoscopic and histological diagnosis were analyzed.Results Redness and erosion under endoscopy,glandular atrophy and chronic inflammation in histological diagnosis were found more frequently in antrum than in corpus.P values were less than 0.001,0.01,0.001 and equaled to 0.025 respectively.Conclusion In functional dyspeptic patients,there are differences in the endoscopic and histological findings between antrum and corpus.Redness and erosion in endoscopic diagnosis,glandular atrophy and chronic inflammation in histological diagnosis are more common in antrum when compared with that in corpus.