BACKGROUND: Fiber post is widely used in front teeth aesthetic repair, but some defect teeth cannot form ferrule or thick root canal, which resulted in fiber post prosthetic replacement defluxion and a failure of repair. Presently, new-style resin cement had self sticking component, showing good effects in sticking of fiber post. Whether combination of new-style resin cement can repair affected teeth using fiber post deserves further investigation.OBJECTIVE: Through fatigue test, residual flexural strength and scanning electron microscope (SEM) observation to compare the strength of severely weakened roots restored with three resin cements. METHODS: Eighteen intact maxillary central incisors were collected and formed to severely weakened canals at the same size. Three kinds of resin cements were used to restore. Rely X Unicem, Panavia F and Super-Bond C&B resin cement and identical glass fiber post were used. All the specimens were restored using Ni-Cr ceramic crown, and placed on TCML chewing machine that loaded 1 200 000 cycle forces, and the cycle times when failure occurred were recorded. The samples were intact following loading received residual flexural strength testl SEM was employed to observe breakage surface of the samples. RESULTS AND CONCLUSION: Mean cyclic loading was significantly greater in the Super-Bond C&B Group (1 200 000 times) than in the Relyx Unicem Group (640 000 times) and Panavia F Group (550 000 times) (P< 0.05). No significant difference was detected between the Rely X Unicem and Panavia F Groups. Residual flexural strength was 747.99 N in the Super-Bond C&B Group. SEM demonstrated that Super-Bond C&B formed longer resin processes, which were more than other two groups. Results confirmed that Super-Bond C&B demonstrated better results when restoring flared roots with prefabricated glass fiber posts.

The afferent connections of the magnocellular preoptic (poma), lateral preoptic (pol), and medial preoptic (pom)nuclei have been investigated with horseradish peroxidase (HRP) method in the rat. Our findings indicated that1. poma mainly receives projection from the rhinencephalic structures including the anterior olfactory nucleus, olfactory tubercle, piriform cortex, entorhinal cortex, the nucleus of lateral olfactory tract, the anterior amygdaloid area, and the cortical amygdaloid nucleus;2. pom mainly receives afferent fibers from some of the medial hypothalamic nuclei;3. pol has the most extensive fiber input, mainly from some of the brain stem nuclei; and4. poma is the only one of the preoptic nuclei which has reciprocal connections with the contralateral forebrain structrues. It is interesting that the poma and pol receive the afferent fibers from different prefrontal cortical regions and the dorsal portion of pom may have input from the lateral reticular nucleus and nucleus tractus solitarii that the ventral portion may not have.In addition, a large number of HRP-labeled cells could be seen in the supraoptic nucleus after injecting any one of the preoptic nuclei and the possible signification was discussed.

BACKGROUND: Some researches report that diffusion-weighted imaging (DWI) can appear abnormal signal in minutes after cerebral ischemia.OBJECTIVE: To investigate the manifestation and regularity of diffusion coefficient in middle cerebral artery occlusion (MCAO) of rats after cerebral ischemia.DESIGN: Randomized controlled study.SETTING: Neurological Department of Clinical College of ChongqingMedical University.MATERIALS: The experiment was conducted at the Radiological Department of the First Affiliated Hospital of Chongqing Medical University from April to July 2004. Totally 18 adult Wistar rats with clean grade were randomly divided into three groups: control group, sham operation group and cerebral ischemia group with 6 rats in each group.METHODS: The origin of right middle cerebral artery was blocked with filament into right internal carotid. In the sham-operated group, the filament was just inserted into the common carotid artery not into the origin of middle cerebral artery, rats in normal control group were not treated with anything after anesthesia. MRI experiments were performed on a 1.5 T GE signa Highspeed NMR spectrometer to obtain DWI and ADC values at the time-point of 7, 15, 30, 60 minutes, 3,6, 12, 24 hours, 3, 7, 15 days, 1 and 2 months after operation.MAIN OUTCOME MEASURES: Changes of ADCs in infarct hemisphere and contralateral hemisphere.RESULTS: There was no significant change in MRI T1-and T2-weighted imaging in rats at 7 minutes post-infarction, but abnormal high intensity was emerged during the period of 1 hour to 3 hours, and enlarged over time. Finally, the infarction area located the right basal ganglia and the frontal-parietal lobes at 12 hours and increased less significantly later.Focal abnormal high signal was noted in DWI 7 minutes after cerebral ischemic insult, and the ADC values was decreased at the same time,reduced gradually after significant recover at 30 minutes, dropped to the lowest level at 12 hours and again increased at 24 hours, since then all the ADC values were remained high throughout follow-up, which increased by 5 times at 2 months than that at 12 hours. Correspondingly,rADC values showed the contrary pattern of evolution.CONCLUSION: It is reflected that ADC is highly sensitive not only to onset of cerebral ischemia but also to chronic cerebral ischemia.

It has been a problem in clinical practice that recurrence and distant metastasis of patients with colorectal cancer undergoing surgery.Recently,pericolonic tumor deposits has attracted extensive attention of scholars,and they think pericolonic tumor deposits may be closely related with colorectal cancer postoperative recurrence and metastasis.This review aims to introduce concept of pericolonic tumor deposits,pathological characteristics and its important role in clinical staging and prognosis,so as to provide clinical doctors medical advice to set more reasonable treatment and achieve the purpose of reducing tumor recurrence or metastasis for patients with colorectal cancer.

The article put across Meta-analysis well by giving its definition and analyzing its development. It empha-sized its effect in the clinical medicine and dwelled on dealing with the problems encountered when meta-analysis wasapplied and the solutions to the problems. It also pointed out its significant role in raising the level of clinical medicine,teaching and scientific research.[

Objective To evaluate the curative effect of the atorvastatin calcium tablet(trade name:lipitor) in curing essential hypercholesterolemia.Methods 76 patients with essential hypercholesterolemia were divided into two groups randomly:43 patients in the research group(lipitor tablet 10mg,qn);33 patients in the comparison group (simvastatin tablet 20mg,qn).The period of treatment for both two groups was 6 weeks.The changes of blood fat were detected before and after taking the medicine.The curative effect were compared at the same time.Resnlts The total cholesterol(TC)of the research groups decreased by 37.06%,the TC of the comparison group decreased by 22.05%.The triacylglyceride(TG)of the research group decreased by 24.81%,however,the TG of the compar- ison group decreased by just 14.49%.The low-density lipoprotein cholesterol(LDL-C)of the research group de- creased by 42.30% while the comparison group just decreased by 28.30%(P＜0,01).Also,the high-density lipoprotein cholesterol(HDL-C)of the research group increased by 7.80%,and the comparison group increased by 7.13%(P＞0.05),The effective rate of the research group was 95.35%.For the comparison group,the effective rate was 90.91%.Conclusion The lipitor has a good curative effect in curing essential hypereholesterolemia.Also, the lipitor has fewer side effect,This medicine is safe and reliable for patients.

Objective To evaluate the effects of nucleoside/nucleotide analogue treatment on immunoglobulin and complement in patients with chronic hepatitis B (CHB).MethodsA total of 157 CHB patients were recruited and divided into CHB group,liver cirrhosis (LC) group and severe hepatitis B (SHB) group.There were 50 patients who received oral antiviral treatment (lamivudine 100 mg/d,or entecavir 0.5 mg/d,or telbivudine 600 mg/d).Serum levels of complement 3 and 4 (C3,C4),C-reaction protein (CRP),hemolytic complement (CH50),immunoglobulin G,M,A (IgG,IgM,IgA),hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) were detected by enzyme-linked immunosorbent assay (ELISA) or immunoturbidimetry.Hepatitis B virus (HBV) DNA was quantified by real-time polymerase chain reaction (RT-PCR) before and 1,2,3 and 4 weeks after nucleoside antiviral therapy.Comparison of means was done by t test and Mann-Whitney test.The correlation was analyzed by Pearson correlation coefficient test.ResultsSerum IgA and IgM levels of SHB and LC patients were significantly higher than those of CHB patients (P＜0.01).Levels of C3,C4,CH50 and CRP were significantly different among three groups.Levels of C3,IgM,IgG and HBV DNA in HBeAg positive patients were significantly different from those in HBeAg negative patients.There was a statistically significant difference of IgA,IgM,C3 and CH50 levels between patients with high HBV DNA level and low HBV DNA level in HBeAg-positive patients.While in the HBeAg-negative patients,only the IgA level was significantly different with HBV DNA levels.After anti-viral treatment,immunoglobulin and HBV DNA levels were all decreased in three groups,while the serum complement level was increased compared to baseline,and the differences became significant at week 4 of treatment. HBV DNA level was negatively correlated with C3 (r=-0.78,P=0.021) and HBeAg titer was positively correlated with C3 (r=0.87,P=0.015).ConclusionsThe immunoglobulin,CRP,C3,C4,and C H50 could reflect the inflammatory activity in liver.The changes of C3 level can predict the efficacy of antiviral therapy.

Cell Hypoxia ; Humans ; Misonidazole ; analogs & derivatives ; Neoplasms ; diagnostic imaging ; pathology ; radiotherapy ; Positron-Emission Tomography ; Radiography ; Radiotherapy, Intensity-Modulated

Objective To incubate rat neural stem cells (NSCs) with selection-specific inhibitor ( LY294002) of PI3-K, and explore the effects of PI3-K/AKT signal transduction pathway in NSCs. Methods NSCs derived from E15-16 rats were isolated and cultured. After incubation of NSCs with different concentrations of LY294002 (0 to 40 μmol/L), cell survival ( WTS-8 assay), proliferation ( BrdU immunohistochemical identification), differentiation (β Tubulin-III immunohistochemical identification) and AKP phosphorylated protein (Western blotting) were detected. Results The effects of LY294002 on the survival, proliferation and differentiation of NSCs was concentration-dependent. The survival of the NSCs decreased significantly with a higher concentrations of LY294002 (25, 30, 35, 40 μmol/L) (P < 0. 05). The numbers of BrdU positive cells with LY294002 of 30, 35 and 40 μmol/L were significantly smaller than that of the control (LY294002 0 μmol/L) (P < 0.05). The numbers of β Tubulin-III positive cells with LY294002 of 35 and 40 μmol/L were significantly smaller than that of the control (P < 0. 05). The expression of AKT was blocked by LY294002 in a concentration-dependent manner. Conclusion PI3-K/AKT signal transduction pathway plays a vital role in the survival, proliferation and differentiation of NSCs.

OBJECTIVE: To summarize the study of bone marrow cell infusion-induced immune tolerance. METHODS: The Pubmed database was researched using the computer for articles published from January 2000 to December 2008 using the key words of "bone marrow cells, transplantation immune tolerance" in English. Simultaneously, Chinese Biomedical Literature Database and China Journal Full-text Database were retrieved for articles published from January 2000 to December 2008 using the key words of "bone marrow cells, immune tolerance" in Chinese. Besides, Organ Transplantation, Transplantation Immune Tolerance and Conference Proceedings of English and Chinese were retrieved by hand. Inclusion criteria: relevant mechanism of immune tolerance; scheme of bone marrow cell infusion-induced immune tolerance; advantages and disadvantages of bone marrow cell infusion-induced immune tolerance; articles in the same circle published in recent years or in authorized journals. Exclusion criteria: repetitive studies or irrelevant articles. RESULTS: Mechanism of immune tolerance comprised cleaning, inability, regulation or inhibition, and ignorance. The scheme of bone marrow cell infusion-induced immune tolerance mainly contained bone marrow cell infusion combined with myeloablative pretreatment, bone marrow cell infusion combined with non-myeloablative pretreatment, pretreatment with immunosuppressive drug or chemotherapeutics, pretreatment of costimulatory signaling blockage, bone marrow cell combined with mesenchymal stem cell infusion. Bone marrow cell infusion-induced immune toleranca could induce long-lasting stable specific immune tolerance by effective immune tolerance mechanism, and had been an effective main method for inducing transplanted tolerance. CONCLUSION: Up to now, clinical immune tolerance is still uncontrollable and facultative. Bone marrow cell infusion-induced stable immune tolerance can develop a new space for organ transplantation.