Objective:To evaluate the effectiveness of procedure for prolapse and hemorrhoids(PPH) in the treatment of severe hemorrhoids.Methods:The experiment group(also called PPH group) consisting of 11 cases was subjected to PPH,the controlled group with 12 cases to Saresola-Klose method.Results:This study showed that the operating time and hospital stay after operation in PPH group were shorter than those in control group;less pain, less complication after operation and faster recovery were found in PPH group.Conclusion:PPH has such advantages as short operative time,less postoperative pain,fast recovery,less complications,etc.But its long-term curative effect is to be observed further.

20?g/L)prior to IPA embolization, and returned to normal after embolization. Conclusion Tumors located posteriorly and superticially in the liver may recruit blood supply though IPA collaterals. Phrenospasm should be cautious during IPA embolization.

OBJECTIVETo investigate application of the bone flap pedicled on the retrograde branch of radial artery for treatment of old scaphoid bone fractures of type AO-B.

METHODSFrom October 2007 to October 2011,41 patients with old scaphoid bone fractures of type AO-B were treated by transplantation of the bone flap pedicled on the retrograde branch of radial artery including 26 males and 15 females with an average of (27.3±4.5) years old ranging from 16 to 43 years old. The courses before operation ranged from 6 to 22 months with an average of 11 months. All fractures belonged to the type B of AO classification, that is old wrist fracture of scaphoid bone. All patients' wrist function (pain, function, motion, grip strength) were evaluated by Cooney's modifiedwrist scoring system before and 6 months after operation,and the conditions of bone healing were observed during the follow-up time.

RESULTSAmong them, 36 patients were followed up from 4 to 15 months with an average of 8.3 months. The wounds were healed well without other complications as infection appearing. X-rays or CT confirmed that all fractures were healed completely. The Cooney wrist score was improved from preoperative 53.61±13.97 to postoperative 81.81±8.71 (P<0.01).

CONCLUSIONThe operation of transplantation of the bone flap pedicled on the retrograde branch of radial artery is an effective method to treat old scaphoid bone fractures,which is scientific and has curative effects, and valuable for clinical application.

Adolescent ; Adult ; Female ; Fractures, Bone ; surgery ; Humans ; Male ; Radial Artery ; injuries ; surgery ; Scaphoid Bone ; blood supply ; injuries ; surgery ; Surgical Flaps ; Wrist Injuries ; surgery ; Young Adult

The establishment of animal model is fundamental to the experimental studies. It involves the selection of animals and cell lines, the determination of the number of cells and the option of the ways of transplant. Only when each production step is operated by following the occurrence and the development mechanism of the gastric cancer, can we simulate the process of growth of the gastric cancer in human body.Due to their congenital immune deficiency, the nude mice serve a better choice for the establishment of gastric cancer animal model. Beyond that, the cell lines selection and transplant way option also play important roles.

Based on the superior optical properties and the advantage of surface-modifiable capacity,the novel fluorescent nanoparticle quantum dots can conjugate with many kinds of biomolecules or chemical materials to form target quantum dots,which play an important role in biomedicine.The target ability this probe possesses offers a more accurate tumor localization,and minimizes the side effect to normal cells while strengthens the curative effect.At present,the probe makes a great progress in many areas,such as cell and molecule recognition,imaging in vivo,biosensing and diseases treating.

Intestinal trefoil factor(IFF) is a low molecular weight polypeptide expressed in intestine.With the research on its structure and physiological function,ITF is beginning to attract attention.This review is mainly about the structure,localization,function,mechanism of action,expression regulation,signal transduction pathway,recombinant expression and so on.

Objective The localization of AQP4 was studied in the brain,especially,in the periventricular organs,such as ependyma,choroid plexues,area postrema(AP) and neurohypophysis,and telencephalon as well as two kinds of endocrine gland:pineal gland and adenohypophysis,so as to provide morphological basis for water transport and balance,the secretion and regulation mechanisms of endocrine gland in the brain. Methods Immunohistochemical staining techniques(LAB-SA,BCIP/NBT) were used. Results AQP4 was most abundantly expressed in the tissues directly contacting with CSF,including the cerebral pia mater,ependyma lining of ventricules and the aqueductal system and choroid plexues.Heavy express of AQP4 was also found in the perivascular glial processes of the brain tissue.In addition,AQP4 was also distributed to the pyramidal cell layer of hippocampus,granular cell layer and polymorphic cell layer of dentate gyrus,adenohypophysis(intermedius included) and pineal gland cells as well as the periventricular organs such as area postrema(AP) and neurohypophysis.Conclusion The localization of AQP4 indicates that AQP4 is involved in not only the transport and balance of water,electrolyte metabolism and the regulation of secretion of the endocrine gland,but also in the neuroendocrine activity of the hypothalamus.

Lymph node mapping, a hotspot in oncology and surgery in recent years, has been successful-ly used in the discovery and resection of the metastatic lymph nodes in patients with melanoma and breast cancer. For the complex of lymphatic drainage and the defect of tracers, lymph nodes mapping is still under investigation. As a new type of nano-materials, the quantum dots are used widely, the tumor lymph node mapping.

In recent years,combined therapy based on radical resection has promoted the prognosis of gastric cancer,but the rate rate of post-surgery recurrence is still high,which is the main cause of death.It helps us take out better treatment for gastric cancer to analyze correlation factors of gastric cancer recurrence after radical resection.It is needed to reviewed the literatures about correlation factors analysis of gastric cancer recurrence after radical resection in recent years.

Objective To evaluate the prognostic value of combined analysis of the biomarker Krüppel-like factor 4 (KLF4) and the Milan criteria in hepatocellular carcinoma (HCC) patients treated by orthotopic liver transplantation (OLT).Method The clinicopathological data and outcome of the recruited 105 HCC patients undergoing OLT from October 2001 to April 2009 were retrospectively analyzed.KLF4 expression in HCC and paired non-tumor tissue was detected by immonohistochemistry and confirmed by Western blotting analysis.Five-year overall survival (OS) and recurrence-free survival (RFS) rate and survival curves of the grouped patients were calculated and plotted by Kaplan-Meier method.Result The level of KLF4 expression was lower in HCC than that in paired non-tumor tissue (P＜0.05).KLF4 expression was significantly lower in poorly differentiated HCC than that in well-moderately differentiated HCC (P =0.008).Loss of KLF4 was an independent risk factor for predicting tumor recurrence and survival of HCC after OLT (HR =0.459 and 5.42,respectively,P＜0.001).The level of KLF4 expression could not differentiate the OS and RFS rate in the patients with tumors meeting the Milan criteria,whereas the OS and RFS rate in the patients with tumors exceeding the Milan criteria differentiated according to KLF4 expression.The patients with tumors beyond the Milan criteria and exhibiting moderate to high KLF4 expression had unexpectedly favorable 5-year OS (91.7％) and RFS (70.5％) rate.Conclusion KLF4 is a useful biomarker for prognostication of HCC patients undergoing OLT.Integrated use of KLF4 biomarker and the Milan criteria improves accurate prediction of survival and tumor recurrence for HCC patients after OLT.