The animal model for the study of B encephalitis was established by injecting Jin Wei Yan 1 strain of B encephalitis virus into the peritoneal cavity of mice. All the mice injected demonstrated the histological features of the disease. Special attention was paid to the ultrastructural changes of the granular cells, the Purkinje cells and the Golgi cells in the cerebellum, and the findings in these cells were compared with those of the nerve cells in the cerebral cortex, dience-phalon and tnesencephalonIt was found that endoplasrnic reticulum could appear in the nerves infected by B encephalitis virus. A radiating structure was-usually formed by the viral replication multivesicular bodies with microvesiculo-tubular body as its center. The morphological changes and the developmental sequence as well as the significance of this radiating structure were discussed.In the late stage of the infection, viral particles were found in the nuclei of a part of the necrotic cells. It was likely that the viral particles entered the nuclei by way of cytoplasm. Our observation confirmed the Chen Liming's hypothesis that viral particles can be formed in the perinuclear space. The way that the viral particles were evacuated from the infected nerve cells observed in this study was in agreement with that reported by Chen Liming, but most of the viral particles left the infected nerve cells through the axons.

Low molecular weight heparin(LMWH)is a kind of anticoagulant used in both the prevention of clot formation in the blood vessels(thrombosis)and the treatment of conditions caused by clot formation or embolization.They are agents formulated from chemical or enzymatic depolymerization of unfractionated heparin.In the past decade,LMWH has been extensively evaluated in many clinical settings.The applications of LMWH in recent years are reviewed.

Humans ; Tinnitus ; therapy ; Transcranial Magnetic Stimulation

Objective: To investigate the efficacy of sinivastatin and aspirin as well as blood pressure controlling therapy for carotid atherosclerosis in patients with hypertension. Methods: 162 patients with essential hypertension and carotid atherosclerosis were randomized into two groups. 82 cases In the treatment group, were treated with simvastatin and aspirin as well as antihypertensive drugs; while 80 cases in the control group were treated with antihypertensive drugs alone. Intima-media thickness (IMT) of carotid arterial, carotid plaques score, blood pressure and the serum lipids were measured before and once a year after treatment for 3 years. Results: Mean IMT of carotid artery and carotid plaques score were (1.01 ± 0.12) mm and 3.8 ± 2.5 respectively in the treatment group 1 year a fter treatment, showed no difference from that before treatment. But they were (0.80 ± 0.16) mm, 2.6 ± 1.6 and (0.80 ± 0.20) mm, 2.5 ± 1.2 after 2 and 3 years respectively, which were reduced significantly compared with that of before treatment. The IMT and internal diameter ratio was also reduced significantly after treatment. Overall incidence of cardio-and cerebro-vascular events in the treatment group were 10.90% and 4.87% during the 3 years follow-up period, which were significantly lowered compared to the control group. Conclusion: Long-term simvastatin and aspirin therapy could effectively delay and reverse the progression of carotid arteriosclerosis in patients with hypertension and prevent cardio-and cerebro-vascular events when combined with antihypertensive drugs.

Objective To investigate the serum level of C reactive protein(CRP) in the hypertension patients with heart function impairment.Methods 68 hypertension patients who had no,mild or severe heart function impairment were se- lected and accordingly divided into 3 groups,while 30 healthy subjects served as normal controls.The levels of HS-CRP and LVEF was measured.Results All the hypertension patients had a high CRP level than normal controls(P

Ear Diseases ; Ear, Middle ; Humans ; Sclerosis

Child ; Child, Preschool ; Humans ; Male ; Posterior Leukoencephalopathy Syndrome

Objective To observe the effect of intravenous injection of different doses of propofol on the ceil apoptosis and NF-kB p65 in the acute lung injury(ALl)induced by LPS in rats.Method Sixty SD rats were randomly(random number)divided into five groups,namely,control(NS)group,Au model group and propofol intervention groups(P1,P2,P3 groups).The lung injury was evaluated by using microscopy with hematoxylin and eosin(HE)staining and arterial blood gas,and Western blotting Was applied to evaluating the nuclear translocation of NF-KB P65 in lung tissues.The apoptosis rate of lung tissue Was determined by flow cytometric analysis.Results Lung injury in model group reached the pathologic criteria of acute lung injury,and it was attenuated apparently in propofol intervention groups(P1,P2,P3 groups)in dose-dependent manner.Western blotting results showed that the nuclear translocation of NF-KB P65 and the apoptosis rate increased significantly in ALI model group compared with control group(P<0.05),and decreased in propofol intervention groups compared with ALl model group(P<0.05).Conclusions Propofol Can attenuate acute lung injury induced by LPS in rats,and significantly inhibit the nuclear translocation of NF-KB P65 and the cell apoptosis in lung tissues.The effect of propofol attenuating acute lung injury induced by LPS in rats may be attributed to the inhibition of nuclear translocation of NF-KB P65and ceil apoptosis in lung tissues.

Objective To compare the expression of renal transforming growth factor-?_1(TGF-?_1),its receptors type Ⅰ and Ⅱ(T?RⅠ and T?R Ⅱ) in two rat models of chronic nephropathy induced by CsA and FK506 respectively.Methods Rat models of chronic CsA-/FK506-induced nephropathy were established by administering sandimun Neoral and Prograf separately.Then their kidneys were dissected and the protein expression of TGF-?_1,T?RⅠand T?RⅡ,the mRNA levels of T?RⅠ and T?RⅡ was detected by using immunohistochemistry(IHC) and in situ hybridization(ISH) respectively.Results The IHC revealed that the integrated optical densities(IODs) of renal TGF-?_1,T?RⅠ and T?RⅡ were(605.24)?(140.24),(876.28)?(208.73) and(981.59)?(258.65) respectively in CsA-treated rats,(488.37)?(101.76),(586.63)?(131.21) and(543.34)?(105.26) respectively in FK506-treated ones.The ISH indicated that the IODs of renal T?RⅠ and T?RⅡ mRNA were(905.08)?(158.75) and(1090.92)?(242.73) respectively in CsA-treated rats,(661.37)?(205.65) and(716.27)?(195.55) respectively in FK506-treated ones.The difference of the above-mentioned five factors between the two groups was significant(P