Objective:To explore the therapeutic effect of nicorandil based on routine medication on stable coronary heart disease (CHD) .Methods :A total of 100 inpatients diagnosed as stable CHD were enrolled ,randomly and e-qually divided into nicorandil group (received nicorandil 5mg based on routine medication ,three times/d) and rou-tine treatment group .After discharge ,patients were followed up for six months .Angina pectoris frequency ,ECG , serum level of high sensitive C reactive protein (hsCRP) and 6min walking distance (6MWD) were compared be-tween two groups before and after follow up .Results:After six-month follow up ,compared with routine treatment group ,there were significant improvements in clinic therapeutic effect (48% vs .74% ) and ECG therapeutic effect (78% vs .96% ) in nicorandil group , P<0.05 all;compared with before follow-up ,there were significant reduc-tions in angina pectoris frequency and serum hsCRP level ;and significant rise in 6MWD in both groups , P<0.05 all;compared with routine treatment group ,there were significant reductions in angina pectoris frequency [ (10.35 ± 1.51) times/week vs .(9.95 ± 1.65) times/week] and hsCRP level [ (1.12 ± 0.51) mg/L vs .(0.95 ± 0.43) mg/L];and significant increase in 6MWD [ (342.38 ± 35.64) m vs .(388.64 ± 32.43) m] in nicorandil group , P<0.05 all .Conclusion:Nicorandil can effectively reduce the attack number of angina pectoris of stable coronary heart disease and serum hsCRP level ,increase exercise tolerance and improve clinical therapeutic effect .

Disability insurance is one of main component of isurance. This paper made comparison of disability standard of insurance among some countries and regions and drawn serveal chracteristics. With the review of Chinese Disability Standard for Insurance (1998 version),implications and strategies had been proposed for further revision.

Objective To screen the conservative,stable and specific DNA signature sequence in the plasmid of Yersinia pestis.Methods Specific validation trials and stability of the qualification test were carried out to 40 strains of Yersinia pestis,47 strains of non-Yersinia pestis of home and wild types of rodent in Yunnan,by using 32 DNA sequences derived from Yersinia pestis in the plasmid and conventional PCR technology,and Yersinia pestis vaccine strain EV76 as a positive control.Results Four pairs of relatively conservative,stable and specific DNA marker genes were screened:YPMT1.05c,YPMT1.03c,YPMT1.42 and YPMT1.04c.Conclusions The 4 pairs of Yersinia pestis DNA signature sequences can be used for rapid diagnosis of plague.

Various column chromatography, such as silica gel, Sephadex LH-20, ODS, and semi-preparative HPLC was used to isolate and purify the chemical constituents from Cinnamomum cassia. The structures were determined on the basis of NMR and MS spectral data analysis, together with the comparison with literature data. Fifteen compounds were isolated from the 85% aqueous ethanol extract of C. cassia, and their structures were identified as (2R, 3R)-5,7,3',4'-tetramethoxyflavan-3-ol( 1), (2R, 3R)-5,7-dimethoxy-3',4'-methylenedioxyflavan-3-ol (2), coumarin (3), cinnamic acid (4), (E)-2-hydroxy-phenylpropionic acid cinnamoyl ester (5), 3, 3', 4, 4'-tetrahydroxy biphenyl (6), methylstictic acid (7), epi-boscialin (8), (1R,2S,3S,4S)-2,3-epoxy-1, 4-dihydroxy-5-methyl-5-cyelohexene (9), 4,5-dihydroxy-3-methyl cyclohex-2-enone (10), cis-4-hydroxymellein (11), and 2-hydroxy-4-methoxyl-cinnamaldehyde (12). Compounds 5-11 were obtained from this genus plants for the first time.
Cinnamomum aromaticum ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Mass Spectrometry ; Molecular Structure

Objective To explore the constituent ratio and clinical characteristics of multifocal thyroid papillary carcinoma (MPTC).Methods The clinical data of 1616 cases of papillary thyroid carcinoma(PTC) were retrospectively analyzed from January 2002 to December 2011 of the First Affiliate Hospital of Dalian Medical University,which operated at the first time and confirmed by pathology.The change of constituent ratio of MPTC in PTC was analyzed and the differences of the clinical characteristics of the multifocal group and single focal group were analyzed.Results The constituent ratio of MPTC in PTC was increasing from 8.33％ (4/48) in 2002 to 30.38％ (96/316) in 2011 gradually.Compared to the single focal group,MPTC group had higher rate of neck lymph node metastasis(45.09％ vs 25.02％ ; P =0.000) and extrathyroidal invasion (20.95％ vs 9.04％ ;P =0.000).Compared with pure microcarcinoma,none pure microcarcinoma had higher rate of neck lymph node metastasis (P =0.000).More than two focuses has higher rate of neck lymph node metastasis than two focuses (P =0.000).The rate of recurrence with lymph node metastasis was higher than that without lymph node metastasis (24.05％ vs 8.98％ ;P =0.000) conformed by postoperative pathology.Conclusions The number and constituent ratio of MPTC in PTC is increasing gradually.MPTC group has high proportion of lymph node metastasis and extrathyroidal invasion than single focal group.None pure microcarcinoma has higher rate of neck lymph node metastasis than pure microcarcinoma;more than two focuses has higher rate of neck lymph node metastasis than two focuses.The recurrence rate is correlated with the rate of lymph node metastasis.

Antibodies ; immunology ; Antibody Formation ; Complement C4b ; immunology ; pharmacology ; Diagnosis ; Graft Rejection ; diagnosis ; immunology ; Humans ; Kidney Transplantation ; immunology ; Peptide Fragments ; immunology ; pharmacology ; Transplantation, Homologous ; immunology

Aged ; Humans ; Male ; Palatal Neoplasms ; pathology ; Uvula ; pathology

Objective To investigate the effects of high-affinity tyrosine kinase receptors TrkA, TrkC and the low-affinity neurotrophin receptor p75 (p75~ NTR) protein in laser-induced retinal injury. Methods The Wistar rats were anesthetized and exposed to frequency doubling Neodymium:Yttrium,aluminum garnet(ND:YAG,?=532nm) laser for 100 plus per eye, which were sacrificed on 12 hours,1,3,7,14 and 28 days after laser exposure and eyeball was taken out. We investigated retinal histology by hematoxylin and eosin staining, and the expression of TrkA, TrkC and p75~ NTR protein was studied by means of immunohistochemistry. Results Immunohistochemistry results showed a differential distribution for these three neurotrophin receptors in the laser injuried retina. TrkA was enhanced in nerve fiber layer (NFL), ganglion cell layer(GCL) and inner nuclear layer(INL) ,which is the most nuclei of the ganglion cells, proximal M?ller cell end feet and a little cells in the innermost part of the INL, its peak of up-regulation was after day 1-3, after day 28 there was sustained. Whereas TrkC and p75~ NTR expression was enhanced in the outer nuclear layer(ONL), which is distal M?ller cell processes, TrkC up-regulated after 12 hours, its peak was on day 1-3, after day 28 there was sustained, p75~ NTR up-regulated after 24 hours, its peak was on day 3, on day 14-28 there was sustained in the GCL, which is proximal M?ller cell processes. Conclusion The expression of TrkA, TrkC and p75~ NTR participated in the course of laser injuried retinal pathology.

Objective To determine the aberrant methylation status of RASSF1A,p16 and DAPK gene promoter region in induced sputum from lung cancer patients and the value of their combined detection in diagnosing lung cancers. Methods Methylation-specific PCR (MSP) was used to detect the promoter methylation status of RASSF1A,p16, and DAPK genes in induced sputum and pathological tissues from 82 patients with lung cancers and 25 patients with pulmonary benign lesion.We also analyzed the relation between methylation status and clinical pathological data.Results The positive rates of promoter methylation of RASSF1A,p16, and DAPK genes in pathological tissues from patients with lung cancers were 63.4%,59.8%, and 58.5%, respectively,and those in induced sputum were 54.9%,48.8%,and 51.2%, respectively. The promoter methylation of RASSF1A,p16, and DAPK genes were not detected in patients with pulmonary benign lesion.There was a significant difference between the lung cancer group and pulmonary benign lesion group (P＜0.05). The methylation rate of RASSF1A gene was significantly lower in the middle and high differentiation and non-metastastic lymph node of lung cancer tissues than that in the poor differentiation and the metastatic lymph node of lung cancer tissues(P＜0.05), and was not correlated with age, sex, smoking index, clinical stage, and pathological types.The methylation rate of p16, and DAPK genes was not significantly correlated with all the above mentioned factors (P＞0.05). The methylation rate of joint detecting RASSF1A, p16, and DAPK genes was 73.2%. Conclusion Joint detection for promoter hypermethylation of RASSF1A, p16, and DAPK genes in induced sputum may be used as a simple and effective index of the diagnosis and prognose of lung cancers, and can improve the positive rate.

Objective To analyze drug resistance data of Enterococcus faecalis and Enterococcus faecium,submitted by member units of Chongqing bacterial drug resistance monitoring network,and to provide the basis for our city effective application of antimicrobial agents and the reference.Methods Target bacteria identification and drug susceptibility test were performed by member units,according to the national technology scheme of bacterial drug resistance monitoring network and the results were determined according to standards published by Clinic and Laboratory Standard Institute(CLSI) in 2015.WHONET5.6 software was used to analyze drug susceptibility,and drug resistance difference was analyzed by using SPSS21.0 software.Results A total of un-repeated 1 811 strains of Enterococcus faecalis and 1 601 strains of Enterococcus faecium,accounting for 13.1% of all positive strains.The resistant rates of the two kinds of bacteria to vancomycin were 0.5% and 1.8%,to rinathiazole amine were 2.5% and 0.5% respectively.Tigecycline resistant strains were not founded.The resistant rate of Enterococcus feaclis to ouinupristin/dalfopristin was 90.1%,to tetracycline was 78.8%,to high concentration of gentamicin was 43.0%,to penicillin,ampicillin and nitrofurantoin was less than 7%.Except ouinupristin/dalfopristin and tetracycline,the resistant rate of Enterococcus faecium to the other drugs were significantly higher than Enterococcus faecalis(P<0.05).Strains isolated from children and adult patients,Intensive Care Unit(ICU) and un-ICU patients were with differences of drug resistance(P<0.05).Conclusion Most of Enterococcus infection could be caused by Enterococcus faecalis and Enterococcus faecium.Monitoring of drug resistance might be helpful for rational and effective usage of antimicrobial agents.