Objective To study the relationship between serum lactadherin (MFG-E8),sialic acid (SA) level and coronary heart disease (CHD) occurrence and development.Methods 166 patients in our hospital were selected from June 2014 to November 2015,and then according to the result of coronary angiography divide the subjects into control group (n=56,the result of coronary angiography completely normal) and CHD group,including stable angina 48 cases (StA subgroup),unstable angina 30 cases (UA subgroup) and acute myocardial infarction 32 cases (AMI subgroup).The serum level of MFG-E8 was detected by ELISA method,the serum level of SA,hs-CRP and lipid index were detected by biochemical analysis.Gensini score showed the severity of coronary stenosis.The correlation analysis between detecting parameters was carried out by Spearman method.Results Compared with the control group,the patients with CHD have a higher level of serum SA and a lower level of MFG-E8 (both P<0.05).The AMI subgroup has a lower level of serum MFG-E8 than StA and UA subgroups,and the AMI subgroup has a higher level of serum SA than StA and UA subgroups (all P<0.05).The correlation analysis showed that the serum SA has a positively correlation with the level of TG,hs-CRP,and Gensini score,and has a negatively correlated with the level of MFG-E8 (all P<0.05).The MFG-E8 has a negatively correlated with the level of TG,hs-CRP,and Gensini score (all P<0.05).Conclusion MFG-E8 and SA play an important role in the development of CHD,and combined detection of serum MFG-E8 and SA may be helpful in the clinical diagnosis and risk assessment of patients with CHD.

Objective To evaluate the safety and effect of extraperitoneal laparoscopic radical prostatectomy for high-risk prostate cancer. Methods From January 2012 to August 2015, 49 patients diagnosed high-risk prostate cancer underwent extraperitoneal laparoscopic radical prostatectomy with 'sandwich' urethra reconstruction. Results None converted to open surgery and the mean operative time was (2.15 ±0.29) h, mean intraoperative blood loss was (60.25 ± 20.29) ml. No rectal injury was observed. The patients were ambulant 1 to 2 days postoperatively. Pelvic lymph nodes metastasis was found in 5 cases. Positive margin was found in 13 cases. Mild urinary incontinence oc-curred in 3 cases. Urethral stenosis occurred in 2 cases. Biochemical relapses occurred on 5 cases during the follow-ing period of 1~43 months for 46 cases. Conclusions Extraperitoneal laparoscopic radical prostatectomy is safe and effective in treatment of high-risk prostate cancer. The very key is to master the anatomy of prostate and laparoscop-ic techniques.

Adult ; Crown Lengthening ; Dental Restoration, Permanent ; Esthetics, Dental ; Female ; Humans ; Incisor ; Malocclusion ; therapy

Objective To analyze the diagnosis of variant acute myocardial infarction.Methods Clinical datas were retrospectively analyzed in 27 cases with variant acute myocardial infarction.Results 27 patients were diagnosed with myodial infarction by ECG and myocardial enzymea.Conclusion Variant acute myocardial infarction is often misdiagnosed as acute abdomen,cerebral aarterial infarction the disease of neck.but its clinical manifestation especially ECG and myocardial enzymes are changeable,and it needs full analysis,early diagnosis and therapy.

Objective To explore the effect of Periplaneta americana reversing multiple drug resistance (MDR) of hepatocellular carcinoma in vitro. Methods Drug resistance of Hepatocellular carcinoma cell line HepG2/ADM was established by Adriamycin ( ADM ) increasing concentration gradient method;determined cell growth curve, doubling time change using Trypan blue staining method;detected sensitivity of 4 kinds of chemotherapeutic drugs of HepG2 and HepG2/ADM by methyl thiazolyl tetrazolium method; detected the non toxic dose of Periplaneta americana CⅡ-3 and the skim cream of HepG2/ADM by MTT method; detected the inhibition rate differences of CⅡ-3, skim cream, ADM, CⅡ-3 and ADM, skim cream and ADM of hepatocellular carcinoma drug resistance cell line HepG2/ADM by MTT method.Results Drug-resistant cell line doubling time was extended obviously compared with parent cells,HepG2/ADM showed resistance to variety of chemotherapeutic drugs, ADM resistant index was especially high,that was 15.25 times.When the concentration of CⅡ-3 and skim cream were 28.76 and 45.08μg/mL respectively, the growth inhibition rate of HepG2 and HepG2/ADM two cell lines were<10%,no obvious toxic effect.The inhibition rate of the drug-resistant cell of 7.5,15,30μg/mL concentration CⅡ-3 joint with ADM concentration (1/2 IC50 ) were (24.52 ±2.05)%,( 34.78 ±3.08)%,( 45.09 ±3.56)% respectively; The inhibition rate of the drug-resistant cell of 12.5,25,50μg/mL concentration skimmed cream joint with ADM concentration (1/2 IC50 ) were (19.28 ±2.56)%,(35.08 ±2.13)%,(43.85 ±3.05)%respectively.Conclusion The cell line HepG2/ADM established has basic multidrug-resistant biological characteristics.Periplaneta americana extract can inhibit the growth of HepG2/ADM,and has good effect to reverse drug resistance at the same time.

Objective To investigate the expression of human telomerase reverse transcriptase(hTRT) in meningiomas,the correlation with malignancy grade and the prognostic significance of hTRT activity.Methods The expression of hTRT and proliferating cell nuclear antigen(PCNA) in 43 meningiomas was examined by immunohistochemical technique.Postive rates of hTRT between histopathologically different grades were analyzed,and so did between initial detected case group and recurrent case group.The labelling indexes of PCNA between hTRT-positive group and hTRT-negative group were also analysed.Results Of 43 meningiomas examined,19(44%) demonstrated the expression of hTRT.hTRT was present in 3 of 26 benign meningiomas(11 5%),10 of 11 atypical meningiomas(90%) and 6 of 6 malignant meningiomas(100%),respectively(P

Integrating visualization toolkit and the capability of interaction, bidirectional communication and graphics rendering which provided by HTML5, we explored and experimented on the feasibility of remote medical image reconstruction and interaction in pure Web. We prompted server-centric method which did not need to download the big medical data to local connections and avoided considering network transmission pressure and the three-dimensional (3D) rendering capability of client hardware. The method integrated remote medical image reconstruction and interaction into Web seamlessly, which was applicable to lower-end computers and mobile devices. Finally, we tested this method in the Internet and achieved real-time effects. This Web-based 3D reconstruction and interaction method, which crosses over internet terminals and performance limited devices, may be useful for remote medical assistant.
Diagnostic Imaging ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Internet ; Software

This study was conducted to investigate the anti-tumor efficacy of nanosecond pulsed electric fields (nsPEFs) on the mouse with A375-GFP melanoma xenograft in vivo. In vivo fluorescence image analysis system was used in this study to evaluate the effects of nsPEFs on human melanoma A375 cell xenograft. On the Day 90 af ter pulse delivery, the skin that had contained A375 cell xenograft was surgically excised and pathologically evalua ted. The changes of scar were recorded by digital camera. The experiment revealed that significant changes in fluorescence value trend and amplitude were found in the treated group from those in the control group. The fluorescence of tumor in the treated group decreased mostly 48 h after the treatment and completely disappeared 10 d after the treatment, while that in control group was increased gradually. Surgical excision of the area confirmed a complete pathologic response. Within a few days after the nsPEFs treatment, a hard scab formed at the treatment region. The scab fell off by the end of the second week. As time went on, the scar gradually became faded and all xenograft tumors were disappeared without recurrence. From the experiment, we learn that nsPEFs can bring good therapeutic effect. It may provide a new approach for the clinical treatment of superficial tumors.
Animals ; Electric Stimulation Therapy ; methods ; Heterografts ; Humans ; Melanoma ; therapy ; Mice ; Neoplasm Recurrence, Local ; Skin ; pathology

Objective To investigate the mechanism of malignant transformation in human bronchial epithelial cell line BEP2D exposed to α-particles.Methods The levels of intracellular ROS and malonaldehyde (MDA) in BEP2D,RH22 (passage 22 of α-particle-irradiated BEP2D cells) and BERP35T-1 cells (derived from nude mice bearing malignant transformed cells generated from the passage 35 of α-particle-irradiated BEP2D cells) were assayed with DCFH-DA and MDA kit,respectively.The expressions of 8-OH-dG and γ-H2AX in BEP2D,RH23 (passage 23 of α-particle-irradiated BEP2D cells)and BERP35T-1 cells were also measured with immunocytochemistry and immunofluorescence staining.Results Compared to BEP2D cells,the levels of ROS ( t =4.30 and 3.94,P ＜ 0.05 ) and MDA ( t =4.89 and 15.10,P ＜0.05) increased in RH22 and BERP35T-1 cells.The expressions of 8-OH-dG (t =3.80 and 2.92,P ＜ 0.05 ) and γ-H2AX ( t =7.61 and 12.67,P ＜ 0.05 ) in RH23 and BERP35T-1 cells were also higher than those in BEP2D cells.Conclusions Oxidative stress induces lipid peroxidation and DNA damage leading to genomic instability,which could contribute to cellular malignant transforming process in the human bronchial epithelial cell line BEP2D with α-particle exposure.

ObjectiveTo explore the correlation between open field test(OFT) and light-dark box (LDB),as two animal models of state anxiety in Kunming mice.MethodsThe behavior of adult,male,Kunming mice in OFT and LDB was recorded by sequence,for five minutes,with a one-week inter-trial interval.The following parameters were evaluated:percentage of time exploring in the OFT central area( OFT-Ctime％ ) ; percentage of squares crossing in the OFT central area( OFT-Ccross％ ) ; total number of squares crossing in OFT(OFT-Cross) ;total number of rears in the OFT(OFT-Rear) ; number of fecal boli in OFT(OFT-FB) ; percentage of time exploring in the LDB light area( LDB-Ltime％ ) ; percentage of squares crossing in the LDB light area( LDB-Lcross％ ) ;and percentage of rears in the LDB light area( LDB-Lrear％ ) ; transitions between two areas in LDB( LDB-Transition) ; total number of squares crossing in LDB(LDB-Croas) ; total number of rears in LDB(LDB-Rear) ; number of fecal boli in LDB (LDB-FB).Subsequently,factor analysis,cluster analysis and correlation analysis were calculated for these parameters.ResultsFactor analysis and cluster analysis of variances from either OFT or LDB revealed a three behavioral dimensions:anxiety factor ( loaded by OFT-Ctime％,OFT-Ccross％ or LDB-Ltime％,LDB-Lcross％,LDB-Lrear％ ),activity factor ( loaded by OFT-Cross,OFT-Rear or LDB-Transition,LDB-Cross,LDB-Rear) and emotionality factor(loaded by OFT-FB or LDB-FB).While all the variances from both OFT and LDB in combination were analyzed,it could be seen as five components:' LDB-anxiety factor ( loaded by LDBLtime％,LDB-Lcross％ and LDB-Lrear％ ),' OFT-anxiety factor ( loaded by OFT-Ctime％ and OFT-Ccross％ ),'LDB-activity factor (loaded by LDB-Transition,LDB-Cross and LDB-Rear),'OFT-LDB-emotionality factor( loaded by OFT-FB and LDB-FB) and 'OFT-activity factor (loaded by OFT-Cross and OFT-Rear).Good correlation were found between OFT/LDB factors alone and in combination,such as OFT-emotionality factor and LDB-emotionality factor(Pearson =0.383,P＜0.05),LDB-anxiety factor and 'LDB-anxiety factor( Pearson =0.989,P＜0.01 ),OFT-anxiety factor and 'OFT-anxiety factor( Pearson =0.934,P ＜ 0.01 ),LDB-activity factor and 'LDB-activity factor ( Pearson =0.956,P ＜ 0.01 ),OFT/LDB-emotionality factor and ' OFT-LDB-emotionality factor ( Pearson =0.835,P＜0.01 ;Pearson =0.696,P＜0.01 ),OFT-activity factor and 'OFF-activity factor( Pearson =0.926,P＜0.01 ).ConclusionEither OFT or LDB comprised three behavioral dimensions:anxiety factor,activity factor and emotionality factor; however,it was difficult to establish face validity as a point-to-point concordance between OFT and LDB.Attention should be paid to heterogeneity of animal models when OFT and LDB were in combination as a behavionomics to evaluate anxiolytics in Kunming mice.