The formation of Staphylococcus epidermidis biofilm on the surface of medical biomaterials may resist the antibiotics treatment and cause chronic infection,which has become a research focus in recent years.Multiple genes constitute complex regulatory network which affect the biofilm formation,and play different roles in the different stages of biofilm formation.Accessory gene regulator (agr) is one of the most important genes in the process of biofilm formation.The process of bacterial biofilm formation,research status of regulation mechanism of agr system and its related genes in the formation of biofilm are reviewed,to provide reference of using agr as a target for the treatment of staphylococcus epidermidis biofilm related infections.

Aim To study the effects of preconditioning with desflurane, sevoflurane and isoflurane on adenosine triphosphate (ATP) in anoxia-reoxygenation myocardial cells. Methods Rat ventricular myocytes, cultured for 4～5 days, were randomly allocated to five groups: Control group, anoxia-reoxygenation group and groups preconditioned with 1.5 MAC desflurane, sevoflurane or isoflurane following anoxia-reoxygenation. The content of intracellular ATP ,the activities of lactic dehydrogenase(LDH) and creatine kinase(CK), and the cell viability were measured at the end of experiment.Results Preconditioning with 1.5 MAC desfllurane, sevoflurane or isoflurane significantly attenuated the great reduction in ATP and cell viability and the increase of LDH and CK caused by anoxia-reoxygenation. There was a positive correlationship between ATP and cell viability,and a negatiue correlationship between LDH and CK (r was 0.83, -0.87 and -0.82 respectively, P

Lycopene—a kind of important active compound of caroteinoids, is greatly beneficial to human health with its diverse biological functions. With the elucidation of lycopene biosynthetic pathway and cloning genes of relative enzymes from microorganisms, it is possible to regulate lycopene biosynthesis via genetic engineering. The biosynthesis pathways of lycopene and gene cloning of lycopene biosynthetic enzymes in microorganisms were reviewed, and gene engineering strains documented in previous works including: E.coli and yeast constructed by genetic recombination, mold strains enhanced the ability of producing lycopene by gene manipulation were summarized. At last, compared with the present methods, the problems existed in the process of construction were pointed out.

Adult ; Carcinosarcoma ; pathology ; Female ; Heart Neoplasms ; pathology ; Humans ; Lung Neoplasms ; pathology ; Myocardium ; pathology

Against the backdrop of ongoing healthcare system reform and escalating competitions among hospitals in China,more and more specialists and scholars on hospital administration begin to focus their studies on the theories and practice of hospital flows.Methodology frequently called into play in such study include out-patient flow refom,progressive flow improvement,and attention to management and service.Discussed in this paper are the advantages and disadvantages of these three methods as a reference for an appropriate choice by hospitals at large.

Objective To investigate the pathological features of rabbit' retina at the early stage of sunlight-induced injury.Methods Twenty New Zealand rabbits without any eye diseases were randomly allocated to 4 groups(5 each): animals in group A were fed under normal illumination environment,the animals in group B were fed under normal illumination environment and exposed to constant simulated sunlight,the animals in group C were fed under low illumination environment,and the animals in group D were fed under low illumination environment and exposed to constant simulated sunlight.The pathological changes in rabbits' retina were observed with light and transmission electron microscope.Results The structure of retina in group A and group C were histologically well-defined,uniform in staining,and with regular cell shape.In the animals in group B,vesiculation occurred in the ganglion cell layer,inner nuclear layer and outer nuclear layer,and some of the mitochondria showed swelling.The intramembranous structure of external segments became disorganized.In the animals in group D,vesiculation and swelling occurred in the ganglion cell layer and inner nuclear layer,with disruption of the cell membrane.Vesiculation and swelling were also found in the outer nuclear layer,with reduction in cell quantity.The structure was disorganized,with disorganized cell membrane,obvious swelling of mitochondria,disorganized intramembranous structure of external segments,and loss and vesiculation of the laminal structure.Conclusion The rabbit' retina will be injured when it is exposed to 30 000 lux constant simulated sunlight for 30 minutes.Rabbits fed in a low illumination environment and exposed to constant simulated sunlight may suffer severer injury than those fed in a normal environment and exposed to constant simulated sunlight.

Objective:To observe the effect of combined Sanjin Tablets and antibiotic on women with urinary tract infection after sex life. Methods: 306 patients were randomly divided into two groups. The treatment group, taking oral SanJin Tablets and intravenous drip of ciprofloxacin, and the control group only ciprofloxacin given by intravenous drip, treatment course lasted for half a month. Results: In the treatment group, the total effective rate reached 96.77% and the rate of symptomatic improvement was 100.00%, and those indexes in the control group can only reached 80.00% and 82.35% respectively. There were obvious difference between the two groups in the rate of total effective rate and symptomatic improvement (P

Objective To explore the clinical effect of renal artery embolization for renal graft dysfunction.Methods A total of 17 patients with renal graft dysfunction were treated by embolizing the renal graft artery.Coil embolization was performed on 7 of the cases,and gelfoam combined with coil was used in the other 10.Results Among the 17 patients,14 discontinued immunosuppressant in 3 weeks after the embolization,and 2 discontinued after receiving low-dose cortisones for about 6 months,and showed good recovery during follow-up.The other patient underwent resection of the renal graft because of continuous fever and graft pain.After the embolization,14 cases developed 'post-embolization syndrome'.Coil migration occurred in 1 case during the procedure.One patient experienced angina during the operation,and developed myocardial infraction the day after the embolization.Conclusions Renal artery embolization is safe and effective for patients with renal graft dysfunction.By using the method,open surgery can be avoided.Attention should be paid to potential complications.

Objective Measuring the therapeutic effect of the isolated duodenal exclusion for type 2 diabetes mellitus rats by observing the post-surgery change of glycometabolism-related indicators which could be contributed to the isolation between food and the duodenal mucosa with an endoluminal sleeve placing into the duodenum intestine duodennal of type 2 diabetes mellitus Rats.Methods In this experiment,12 male type 2 diabetes mellitus Goto-Kakizaki (GK) rats were randomized to endoluminal exclution as the experimental group and 12 to sham surgery as the control group,The body mass,the average daily food intake and fasting plasma glucose were determined before (0 week) and 1,3,6,12 weeks after operation;For testing the hematoglobinA1c(HbA1c) level,blood samples were collected before and 6,12 weeks after operation.Results No statistical significant differences between two groups before operation index.In both groups,The body mass and average daily food intake decreased markedly at 1 week after surgery:the experimental group body mass preoperative(262.6 ± 5.6 g)vs postoperative(224.0 ± 6.3 g) ;the average daily food intake preoperative(25.5 ± 2.7 g) vs postoperative (16.5 ± 3.0 g),P ＜ 0.05.Changes in the experimental group with control group,P ＜0.05.The 3 week,6 week,12 week body weight was gradually increased in rats of two groups.The experimental group daily feed intake is always lower than the preoperative postoperative,P ＜ 0.05.In the 3 weeks,the control group's body weight and daily food intake were higher than those before operation,P ＜ 0.05.The experimental group post-FPG level (7.5 ± 1.1) mmol/L,(7.2 ± 1.2) mmol/L,(7.3 ± 0.9) mmol/L,(7.1 ± 1.0) mmol/L vs preoperation (12.2 ± 1.2) mmol/L were decreased significantly,P ＜ 0.05.Compared to the preoperation,the HbA1c concentration of the experimental group decreased significantly,6 w (7.8 ± 0.9) ％,12 w(8.2 ± 1.2) ％ vs the preoperation (10.3 ± 1.4) ％,P ＜0.05.These indicators of the control group showed no significant changes,P ＞ 0.05.Conclusion The isolated duodenal exclusion can achieve the therapeutic effect for type 2 diabetes mellitus on GK rats,and which might be attributed to the improvement of the glycometabolism.

Objective To study a new method for internal drainage and primary suture after laparoscopic choledocholithotomy (LCH). Methods Twenty-five patients with chronic cholecystitis combined with cholecystolithiasis and choledocholithiasis were performed laparoscopic cholecystectomy and choledocholithotomy and extraction of stones with choledoscopy. Results One case of Mirizzi' syndrome converted into laporotomy and 24 cases were successfully performed. Excluding neither internal nor external drainage was carried out with primary closure of common bile duct (CBD) in 1 case and T-tube were placed in 4 cases, the made-self 3mm～5mm diameter silica tubes with one-way hook were placed into the common bile duct for internally drainage in 19 cases. The time of operation was from 90 to 420 minutes with a mean of 145 minutes. 1～5 calculus were removed from CBD. A mean of hospitalized days stay was 7.5 day. Conclusions The operation of internal-tube drainage of LCH frees either the inevitably risk of hyper pressure of biliary duct because of the spasm of Oddi's sphincter or disadvantage of T-tube for drainage after LCH, which is new method both safety and practicality, both simpleness and easiness, and accords with the physiological needs of the human body.