This article presents a technique of chromosome analysis which are prepared from human endometrium directly. The success rate was 40.3%. It was shown that there was no statistical significant differences between mitotic index of prolifera-tive and secretory phases. The same study had been carried out in women before and had been taking clomiphene for 3 cycles and 6 cycles. And also no detectable mutagenic, and no carcinogenic effects on endometrium were found

Objective To discuss the reasonable utilization of β-lactam antibiotics in patients with renal failure on hemedialysis. Methods The clinical characteristics of patients with renal failure on hemodylysis,who de-veloped the adverse effect of nervous system and psychiatric symptom while being treated with β-lactam antibiotics,were retrospectively analyzed. Results The adverse effect of nervous system and psychiatric symptom showed dur-ing and after hemodialysis,including: mental confusion,hyperexcitabiliy,incoherent speech,lethargy and amyestasia in two cases. Physical examination did not show signs of cerebral focal injury in all cases. Computed tomography of brain in 7 patients did not show acute lesions. The symptoms completely disappeared after withdrawal of β-lactam antibiotics but bemodialysis or hemodiafiltration continued. Conclusion β-lactam antibiotics used in conventional dose could cause temporary adverse effect of nervous system in patients with renal failure on bemodialysis,which is related to prolongation of drug half life.

BACKGROUND:With increasing freeze-drying time,the water content of allogeneic bone reduces along with the bone mechanical strength.The suitable dehydration degree that maintains mechanical strength of allogeneic bone and allows long-term preservation at room temperature remains controversial.OBJECTIVE:To detect the mechanical properties of bovine bone nail following different degrees of dehydration using the three-point bending mechanics tests.DESIGN,TIME AND SETTING:Biomechanical analysis,comparative observation.The experiment was performed at the Mechanical Laboratory of Southern Medical University between May 2007 and May 2008.MATERIALS:Bone nails,50 mm length and 3 mm diameter,made from healthy adult bovine femoral cortical bone were divided into control and freeze-drying groups (8,12,24 hours,respectively) with 20 samples in each group.METHODS:The water content of bone nails with or without freeze-drying was measured by SH10A Moisture Quick Determination Meter.Three-point bending mechanics tests were performed using MTS 858 Mini Bionix.The parameters included load range 0-2000 N,resolution 0.01 N,cross beam replacement resolution 0.001 mm,span 50 mm and loading speed (cross beam replacement speed) 2 mm/min.MAIN OUTCOME MEASURES:Water content,maximal load and elasticity load.RESULTS:The water content of control group was 7%-9%,and following freeze-drying for 8 hours,the water content was 5.5%-6%,which met the standard of freeze-drying bone;Compared with control group,the maximal load and elasticity load reduced respectively 0.004% and 0.000 8% (P＞0.05).Following freeze-drying for 12 and 24 hours,the water content was 3.5%-4% and 1%-1.5%;the maximal load reduced 21% and 37% and the elasticity load reduced 22.5% and 39% compared with control group (P＜0.05).CONCLUSION:Freeze-drying for 8 hours is the best time of dehydration.The mechanical load and elastic modulus reduce with increasing freeze-drying time.

Objective To investigate the possible mechanisms of ox-LDL on vascular smooth muscle cells (VSMC) with and without exogenous zinc finger protein A20 gene. Methods VSMC were trans-fected with plamid containing A20 gene and incubated with ox-LDL. TLR4 and LOX-1 mRNA expression were measured by RT-PCR. Results After VSMC incubated with ox-LDL, the expression of LOX-1 and TLR-4 mRNA reached a higher level. However, when VSMC were transfected with plasmid containing A20 gene, the NF-kB nuclear translocation and the ability of migration reduced to normal level. Conclusions ox-LDL can significantly activate NF-kB signaling system and enhance migration in VSMC. The activation may induce the inflammatory response in arterial wall, the migration of VSMC to the intima, and triggering the process of atherosclerosis. Transfecting plasmid containing A20 gene significantly inhibited the activation of NF-kB and the ability of migration of VSMC. A20 effects might be through inhibiting NF-kB signaling. Meanwhile, A20 blocked the development of atherosclerosis.

Apoptosis ; Child ; Cytokines ; immunology ; secretion ; Humans ; Systemic Inflammatory Response Syndrome ; immunology ; therapy

Objective To study the effect of adjuvant transcatheter arterial chemoembolization (TACE) on intrahepatic recurrence after curative liver resection for primary hepatocellular carcinoma (PHC).Methods 130 PHC patients who underwent curative liver resection from June 2010 to February 2013 were selected and randomly allocated to the study group (n =67) and the control group (n =63).The study group underwent adjuvant TACE for 1 ～ 4 times,and the control group did not receive any adjuvant therapy.On follow-up for 36 months,the postoperative recurrence rates,tumour free survival time and tumor free survival rates of the two groups were compared.Results At 6 months after surgery,the serum AFP level [(379.8 ±91.7) μg/L vs.(414.5 ±84.3) μg/L] and the IGFBP-2 level [(2 855.3 ±727.5) μg/L vs.(3 259.6 ± 894.1) μg/L] in the study group were significantly lower than the control group (P ＜ 0.05).The 1-year cumulative recurrence rate in the study group (10.4％ vs.28.6％) was significantly lower than the control group (P ＜ 0.05).However,the 2-year and 3-year cumulative recurrence rates were not significantly different between the two groups (P ＞ 0.05).The 3-year cumulative tumor free survival rate of the study group (81.0％ vs.73.2％) was significantly higher than the control group (P ＜ 0.05).Patients in the study group with portal vein tumor thrombus had an 1-year cumulative recurrence rate of (12.8％ vs.31.6％) and a 2-year rate of (28.2％ vs.50.0％).In patients with a preoperative AFP ≥400 μg/L,the 1-year cumulative recurrence rates were (8.8％ vs.30.6％).When the tumor diameter was ≥10 cm,the 1-year,2 year and 3-year cumulative recurrence rates were significantly lower than the control group (P ＜ 0.05,11.8％ vs.46.7％),(35.3％ vs.75.3％) and (47.1％ vs.86.7％),respectively.The recurrence rates in patients who had no cancer thrombus,a preoperative AFP ≤400 μg/L and a tumor diameter ＜ 10 cm were not significantly different between the two groups (P ＞ 0.05).The recurrent rates of patients who underwent TACE 1 time and those who did not receive TACE showed no significant difference (P ＞0.05).In patients who had TACE twice,the 1-year (4.3％ vs.28.6％),2-year (15.2％ vs.41.3％) and 3-year (26.1％ vs.49.2) cumulative recurrence rates were significantly lower than patients who did not receive TACE (P ＜ 0.05).In patients who had TACE for more than three times,the cumulative recurrence rate was similar to patients who had TACE twice (P ＞ 0.05).Conclusions Adjuvant TACE helped to reduce PHC recurrence after curative liver resection during the high-risk period for recurrence.A 1-time TACE did not significantly reduce postoperative recurrence rate,and ≥ 3 times TACE did not significantly improve tumor-free survival rates.Adjuvant TACE and the number of TACE should be reasonably carried out based on the indications and pathological characteristics.

Adult ; China ; epidemiology ; Congenital Abnormalities ; epidemiology ; Female ; Humans ; Incidence ; Infant, Newborn ; Maternal Age ; Pregnancy ; Time Factors

Neutrophil gelatinase associated lipocalin(NGAL),also known as lipocalin 2,has close relationship with human neoplasias,which is up-regulated in a number of malignancy and involves in the process of tumor development.But it appears to exist paradoxical effects attributed to NGAL in human neoplasias.For instance,through protecting MMP9 from autodegradation,NGAL has a clear pro-tumoral and pro-metastatic effect,as has already been observed in breast cancer and esophageal cancer.However,on the contrary,for pancreatic cancer and ovarian cancer,NGAL can inhibit FAK phosphorylation and vascular epidermal growth factor synthesis,having an anti-tumoral and anti-metastatic effect.This review summarizes the current knowledge about the dual effects of NGAL in the process of tumor development,and attempts to explore the possible underlyins mechanism.

Background Recent research showed that stromal cell-derived factor-1 (SDF-1) plays critical role in mediating inflammation,metastasis of tumour and neovascularization of tumour.However,There is still no report about the research of the effects of SDF-1 on alkali-burn-induced corneal neovascularization (CNV).Objective The aim of this study attempts to investigate the role of SDF-1 and chemokine receptor CXCR4 in alkali-induced corneal neovascularization in mice.Methods Alkali-induced-corneal neovascularization animal models were constructed of in 15 eyes of 15 clean C57/BL mice by placing the filter paper with 1 mol/L NaOH to the central cornea for 10 seconds.The animals were sacrificed and specimens of cornea were obtained in 3,7,14 days after alkali burn of cornea.The expression of SDF-1/CXCR4 protein in the corneal tissues was detected by immunohistochemistry and Western blot,and expression of SDF-1/CXCR4 mRNA was detected by reverse transcription PCR (RT-PCR).Results SDF-1 was absent expressed and CXCR4 was faintly stained in normal cornea.The expression level of SDF-1 and CXCR4 was significantly enhanced in different time points after alkali burn in comparison with normal control mice by RT-PCR(P＜0.05),and the same trend was seen in the expression level of SDF-1 and CXCR4 proteins by Western blot (P＜0.05).The expression level of SDF-1 and CXCR4 in cornea peaked in the seventh day and began to decline in the fourteenth day but was still higher than normal level.Conclusion The study indicates that SDF-1/CXCR4 plays an important role in the formation of corneal new vessel in alkali-burn-induced animal model.

Objective:To explore the effects of nutritional intervention on the nutritional status,cognitive function and activities of daily living (ADL) in patients with Alzheimer's Disease (AD).Methods:90 AD patients were randomly divided into the control group and the intervention group,with 45 cases in each group.Patients in the control group received anti-dementia drugs,and patients in the intervention group additionally received TPF-T as the oral nutritional supplement.The nutritional status and nutritional biochemical indicators,cognitive function and ADL of two groups were evaluated when enrolled and 6 months later after the treatment.Results:After 6 months'treatment,the nutritional status,MMSE,ADL scores and nutritional indicators of patients in the intervention group were significantly improved compared to the control group (P ＜ 0.05).Conclusion:Nutritional intervention can effectively improve the nutritional status,cognitive function and ADL of patients with dementia.