Objective:To evaluate the profile esthetics of children with skeletal class Ⅲ malocclusion treated with micro-implant.Methods:20 patients (12 boys and 8girls) aged 11-13 years were treated by micro-implant and maxillary protraction for 8 to 10 months.The profile esthetic indexes were measured on pre-and post-treatment cephalometric radiography.Results:The esthetic of the patients were remarkably improved after treatment.The factors that influence the esthetic index of children were the anteroposterior relationship of the maxilla,the mandible and the thickness of soft tissue.The results were stable 1 year after treatment.Conclusion:Maxillary protraction by micro-implant can improve the profile esthetic of children with Class Ⅲ malocclusion,correct over-bite and over-jet.

BACKGROUND: Wu long dan is the experienced formula summarized in the long-term clinical practice on the treatment ischemic cerebral vascular disorder and aims at "benefiting qi,strengthening spleen,tonifying kidney,activating blood circulation,promoting circulation of collaterals and resolving phlegm". It is indicated in the previous researches that such formula acts on improving microcirculation of cerebrum,regulating neural endocrinal system and being against cerebral ischemic injury. The increased blood viscosity and disturbance endothelin and nitric oxide(NO) secreted from vessels and nerve cells have participated in the pathological progression of multi-infarct dementia(MID) and affect mutually.OBJECTIVE: To probe into the effect of wu long dan on blood viscosity,NO and endothelin in MID model in rats by duplicating MID rat model.DESIGN: A randomized controlled and experimental study based on the experimental animals.SETTING: Department of traditional Chinese medicine in a military medical university of Chinese PLA.MATERIALS: The experiment was performed in Laboratory of Department of Traditional Chinese Medicine in First Military Medical University of Chinese PLA and Laboratory of Department of Neurological Internal Medicine in affiliated Zhujiang Hospital from January to August 2002. SD male rats of clean grade were employed,weighted(270 ± 30) g (Qualified No. 2000337). In the experiment,there were blank control,model group,wu long dan low dosage group and wu long dan high dosage group.METHODS: After internal carotid artery isolated,dried blood suspension of homologous rats was injected at the ratio of 1:200 to prepare MID rat model. Cone-plate stationary method was adopted for the determination of blood viscosity,radioimmunoassay was for the determination of endothelin and cadmium-reduction-colorimetric method was for NO determination. Water-alcohol sedimentation was for the preparation of wu long dan.MAIN OUTCOME MEASURES: Primary indexes: whole blood viscosity,plasma viscosity,plasma endothelin and NO concentration in every experimental group. Secondary indexes: results of learning and memory test.creased significantly (200 s-1: 7.21 ± 1.02; 5 s-1: 11.24 ± 0.93,P ＜ 0.01 ),the plasma endothelin(ng/L) content(167.91 ±46. 87,P ＜0.01) and endothelia/NO(64.94 ± 11.14,P ＜0.01) were increased,and NO(μmol/L)(2.60 ± 0. 43,P ＜ 0.01 ) reduced. The significant difference in the vismedication group in rats(in high dosage 200 s-1: 4.28 ±0. 81,5 s-1:8.84±0.79,P ＜0.01; in low dosage 200 s-1: 5.22±0.92,5 s-1;9.18 ± 0. 81,P ＜ 0.05 ) with model group. Concerning to plasma endothelin(ng/L) (in high dosage 120. 18±34.51,P ＜ 0.01),NO (μmol/L)content(6.84 ± 0.79,P ＜ 0.01 ) and endothelin/NO(31.26 ± 8.41,P＜ 0.01 ),the significant differences presented in all of those compared with model group.CONCLUSION: The increased blood viscosity and the disturbance between endothelin and NO secreted from vessels and nerve cells have participated in the pathological progression of multi-infarction MID and affect mutually. Wu long dan with the efficacy of strengthening spleen and tonifying kidney,activating blood circulation,resolving phlegm and promoting the circulations of collaterals acts on antagonism and regulation of increased blood viscosity and abnormal secretion of NO and endothelin in MID model rats.

It introduces the automation system in the building of hospital and the monitoring principle of site elements.The design and the experience are mainly described.

Objective To investigate the value of the model for end-stage liver disease (MELD) and Child-Turcotte-Pugh (CTP) score in predicting the prognosis of patients with liver cirrhosis.Methods Seventy patients with liver cirrhosis were selected.The MELD and CTP score before surgery was calculated and was analyzed the correlation between the two models was analyzed.The prognosis ability by the area under the receiver operating characteristic (ROC) curve was evaluated.Results Twenty three cases (32.9％,23/70) appeared post operative serious complication.The scores of MELD and CTP in complication group (23 cases) was (19.58 ±5.90),(8.84 ± 1.87) scores,the scores of MELD and CTP in without complication group (47 cases) was ( 12.27 ± 2.94),(6.10 ± 1.12) scores,there were significant differences between two groups (P ＜ 0.01 ).According to the MELD score,70 patients was divided into ＜ 14 scores group(30 cases),14 - 23 scores group(28 cases),＞ 23 scores group( 12 cases),the rate of complication was 10.0％(3/30),35.7％( 10/28 ) and 83.3％(10/12),there were significant differences among three groups(P＜ 0.05).According to the CTP score,70 patients were divided into A grade(29 cases),B grade (25 cases) and C grade( 16 cases),the rate of complication was 10.3％ ( 3/29 ),36.0％ (9/25) and 68.8％ ( 11/16 ),there were significant differences among three groups (P ＜ 0.05 ).The results of Pearson correlation analysis showed that the MELD score and CTP score had significant correlation (r =0.874,P ＜ 0.01 ).The area under the ROC curve of the MELD score and CTP score in prognosis the perioperative complication was 0.877 (95％ CI:0.84 - 0.95 ) and 0.852 (95％ CI:0.83 - 0.94),there was no significant difference ( U =0.157,P ＞ 0.05 ).Conclusion Both MELD and CTP score can accurately predict the short term prognosis of patients with liver cirrhosis.

Objective To study the expression of interferon regulatory factor 1 (IRF-1) in patients with systemic lupus erythematosus (SLE) and the influence of prolactin (PRL) upon it. Methods The level of serum PRL in quiescent condition was examined by electrochemiluminescence-meter in 30 patients with SLE and 20 healthy controls. Peripheral blood mononuclear cells (PBMC) were separated from all the subjects by gradient centrifugation density, and cultured with or without the presence of recombinant human PRL (rhPRL) for 24 hours. The expression of IRF-1 gene in cultured PBMC was detected by reverse transcriptase-PCR (RT-PCR) with gel image scanning. Results The relative value of IRF-1 gene expression was significantly higher in SLE patients than in normal controls (0.89±0.21 vs 0.78±0.18, P=0.026), and in SLE patients with high PRL than in those with normal PRL (1.06±0.26 vs 0.82±0.21, P=0.005). However, there was no significant difference between SLE patients with normal PRL and healthy controls in regard to the expression of IRF-1 gene (P=0.514). The stimulation with rhRPL significantly elevated the relative expression of IRF-1 gene in SLE patients with normal PRL (0.99±0.22 vs 0.82±0.21, P=0.036), but had no obvious effect on that in the normal controls. Conclusion The study reveals a high expression of IRF-1 gene in SLE patients, which may be related to the high level of PRL.

Objective To evaluate the effect of perioperative intravenous (Ⅳ) iron sucrose therapy on reducing postsurgical blood transfusion rates in elderly patients with hip fractures.Methods From September 2011 to February 2014,200 patients aged≥65 years with hip fractures were enrolled.The iron sucrose group (n=100) received iron sucrose (600 mg,Ⅳ),while the control group (n=100) did not receive iron sucrose.Postsurgical blood transfusion rates,infection rates,mortality and length of hospital stay were evaluated.Results The difference in blood transfusion rates was significant (25.0％ vs.41.0％,P＜0.05),while differences in infection rates,mortality and length of hospital stay were not significant between the two groups.Conclusions Perioperative Ⅳ iron sucrose can reduce blood transfusion requirements in elderly patients with hip fractures.

0.05).These findings indicate that severe burns can destroy the coupled phosphorylation in the mitochondria and impair their capability to produce ATP.Immediate fluid infusion after burns can protect the mitochondrial function effectively.

The physical and chemical factors affecting the allergenic potency of the house bdust mite Dermatophagoides farinae extract were determined by skin test and ELISA technique in the asthmatic patients sensitive to the mite. The results showed that the allergenic potency of the extract could be reduced by heat sterilization, trypsin trea-tment and lyophization,but not lost completely,and that the potency could be increasedby supersonic treatment and repeated freeze-thaw(but less than 10 times).It was alsoshowed that the allergenic potency of the extract preserved at -20℃ was more stablethan those preserved at 4℃ or at room temperature,and that the mite extract preservedat 4℃ more than 9 years still had allergenic potency.

Objective To investigate the clinical value of self-made dressing negative pressure drainage technique in the treatment of the four limbs soft tissue trauma.Methods A total of 60 patients with the four limbs soft tissue trauma in our hospital from December 2013 to December 2014 were randomly divided into the observation group and control group.The observation group with 30 cases were treated with the self-made negative pressure drainage technique,and the control group with 30 cases received the conventional treatment.The pain score,wound healing rate,complication rate between two groups were compared.Results The pain score of the observation group was(1.17±0.22),which was lower than(3.17±0.49) of control group,the difference was statistically significant(P<0.05).The wound healing rate of observation group(73.33%) was higher than that of the control group(40%),the difference was statistically significant(P<0.05).The complication rate of observation group(3.33%) was lower than that of control group(23.33%),the difference was statistically significant(P<0.05).Conclusion The self-made dressing negative pressure drainage technique applied to the patients of four limbs soft tissue trauma can reduce the pain,shorten the healing time,and reduce the incidence of complications.

This study was aimed to analyze the clinical features of paroxysmal kinesigenic dyskinesia (PKD) and extend the understanding of this disease. From August, 2008 to October, 2010, 9 patients were diagnosed with PKD in the Department of Neurology of the First Affiliated Hospital of Zhejiang University, China. The data involving clinical demographic characteristics, somatosensory evoked potentials, results of electromyography, video electroencephalography (EEG), brain magnetic resonance imaging (MRI) and computerized tomography (CT) were collected. All PKD patients exhibited unilateral or bilateral recurrent episodic dyskinetic attacks triggered by sudden voluntary movements. The duration of the attacks ranged from several seconds to one minute. The attack frequency ranged from approximately once in several months to more than 10 times in a day. Patients suffered from no conscious disorders during the attack, and no neurological signs were found during the period between attacks. No abnormal somatosensory evoked potentials were found. Routine EEG, video EEG monitoring or brain imaging showed normal findings. Classical treatment for anti-epilepsy, including carbamazepine and topiramate, was administered to the patients and proved to be effective. It was concluded that PKD is characteristically triggered by sudden voluntary movement; no abnormal electroneurophysiological findings are observed in PKD, and antiepileptic drugs are effective in treating the disorder.