Objective To study the surgical treatment strategy for elderly(aged at 80 years or over)colorectal cancer patients.Methods There were 65 cases of colorectal cancer undergoing Surgical treatment in Beijing Cancer Hospital from 1999 to 2006,and results were analyzed retrospectively.Results In this group of patients.the postoperative morbidity was 53.8%,but anastomotic leakage rate was only 3.9%,operative mortality was 1.5%.Postoperative 1-,3-and 5-year survival rate was 78.92%,27.79%,and 16.32%respectively.Kaplan-meier analysis and Cox regression analysis showed that:the TNM stage of the tumor and preoperative hemoglobin and WBC level were independent prognostic factors,but patients' age,gender,preoperative serum level of CEA and albumin,tumor differentiation,tumor size were not independent prognosis factors.Conclusion Elderly CRC patients have a higher risk of surgical treatment.But with the improvement of perioperative management,the anastomotic fistula and mortality rate were not increased significantly and the result is satisfactory.

Objective To analyze the polymorphism of thymidylate synthase(TS)in colorectal cancer patients,further more,to provide guidance for personalized therapy of colorectal cancer.Methods PCR direct sequencing was used to detect the polymor-phism of TS in 252 patients with colorectal cancer.Results TS genotypes of 252 patients with colorectal cancer were detected total-ly,including 137 male and 115 female.3RG/3RC accounted for the largest proportion in both male and female(36.50% and 36.52%respectively).In female,2RC/3RC and 3RG/3RG both accounted for the second largest proportion(both 18.25%).While in fe-male,3RG/3RC accounted for the second largest proportion(26.09%).If patients were divided according to age groups,in youth patients(n=28),3RG/3RC accounted for the largest proportion(42.86%),and the second was 2RC/3RG(21.43%).In the middle aged patients(n=84),3RG/3RC(45.24%)and 2RC/3RC(16.67%)were the major genotypes.For old patients(n=115),the ma-jor genotypes were 3RG/3RC(36.52%)and 2RC/3RG(16.52%).Conclusion The polymorphism of TS are mainly 3RG/3RC in colorectal cancer patients.

Objective To observe the effect of two different methods of fixed temporary pacemaker electrode. Methods Fromn April 2015 to December 80 temporary pacemaker patients were randomly divided into control group (n = 40) and the experimental group (n = 40), the control group using gauze cloth tape cross method combined with the traditional fixed, the experimental group was treated with 3M type of comfortable and stable transparent dressing+3M pressure fixing belt fixed, to compare two groups in the skin lesions, electrode dislocation, inflammatory reaction, the switching frequency difference. Results There was no significant difference between the two groups in general data (P>0.05). There was no significant difference between the two groups in the cost of dressing and materials、electrode dislocation (P>0.05), experimental group of the switching frequency is lower than the control group (Z=-2.064, P<0.05), the differences were statistically significant. Conclusions 3M comfortable type solid transparent sticking and 3M pressurized fixed band method in the absence of increased medical costs while ensuring medical safety, save the manpower cost.

Objective To analyse the effect of hedyotisdiffusa on expressions of apoptosis related protein Fas , caspase3 and caspase7 in Renca renal cell carcinoma of model mice.Methods One hundred and twenty BALB/C male mice were randomly divided into normal control group, model control group, interleukin group, hedyotisdiffusa group, 30 mice in each group, half male and female.Excepted for normal control group , renal cell carcinoma models were established in the other groups , and were given corresponding drug treatment.The tumor size and weight , and the tumor inhibition rate of all mice were observed, and the expression levels of Fas,FasL,caspase3 and caspase7 were detected.Results Compared with model control group, the tumor size and weight were lower , the tumor inhibition rate were higher , the positive expression rates and protein levels of Fas were higher , the positive expression rates and protein levels of FasL were lower, the positive expression rates and expression levels of Caspase3,Caspase7 protein were higher in the mice of interleukin group and hedyotisdiffusa group, all with significant differences ( P<0.05 ).The above indicators in hedyotisdiffusa group were significant improved compared with interleukin group (P<0.05).Conclusion The hedyotisdiffusa can effectively promote the expressions of apoptosis related protein Fas,caspase3 and caspase7 in renal cell carcinoma of model mice, inhibit the expression of FasL protein, improve the tumor suppression rate.

30%. For inferior genicular artery lesions,only balloon angioplasty were carried out. All patients were followed up for 6 months and 12 months to assess of restenosis and clinical efficacy. Results All the patients' therapy was successful,their clinical symptoms were improved or disappeared. The 6-month follow-up showed that restenosis rates were 5.5%,16.7% and 25% respectively in the iliac artery,femoral popliteal artery,and inferior genicular artery. The rates in 12-month follow-up were 11.1%,33.3%,and 75% correspondingly. Conclusion Angioplasty and stent implantation are safe and effective in treatment of lower limb atherosclerosis obliterans. Standardized postoperative anti-platelet,anticoagulant,and anti-lipid therapy is essential to maintain blood vessel recanalization.

Acupuncture Therapy ; Adult ; Aged ; Aged, 80 and over ; Combined Modality Therapy ; Exercise Therapy ; Female ; Humans ; Male ; Middle Aged ; Osteoarthritis, Knee ; therapy ; Treatment Outcome

Education, Nursing, Continuing ; General Surgery ; education ; Humans ; Physicians

Objective To characterize the effects of AQP1 expression on kidney damage in rat disseminated intravascular coagulation(DIC) caused by lipopolysaccharide(LPS) dosing. Method Fifty male Wistar rats (clean grade) were randomly assigned into 5 groups of 10 rats. The 10 control rats were dosed with 10 ml of 0.9%NaCl solution by a drip via the vena caudalis within 4 h, and blood and tissues were obtained after treatment completion. In the DIC groups, the rats were dosed with LPS (30 mg/kg body weight in 10 ml of 0.9% NaCl solution) by a drip via the vena caudalis within 4 h, and blood and tissues were obtained at 4, 6, 8 and 10 h. The blood platelet(PLT) count, prothrombin time(PT) , activated partial thromboplastin time(APTT), fibrin(FIB) and D-dimer(D-D) were detected. Hematoxylin and eosin(HE) staining was used to examine the pathologic changes in the lung and kidney tissues of each group (both hematologic parameters and tissue pathologic changes were used to judge the course of DIC). The AQP1 gene expression levels in the kidney tissues from the groups were evaluated by the mRNA levels using RT-PCR. Statistical analyses were performed by the SNK- q method. Results The PLT count, PT, APTT, FIB and D-D examinations revealed remarkable changes in all DIC groups compared with the control group (P < 0.01). The AQP1 mRNA level was significantly decreased in the DIC group at 4 h compared with the control group (P < 0.01) , and further decreased to the minimum level in the DIC group at 6 h. Moreover, cloudy swelling of renal tubular cells was observed at 6 h and cell degeneration and necrosis were observed at 8 h among the DIC groups. Conclusions Downregulation of AQP1 mRNA expression occurred before damage to the renal tubular cells in DIC, indicating that AQP1 expression may be involved in the kidney damage observed in rat DIC.

OBJECTIVE: To investigate the arsenic speciation of Niuhuang Jiedu Tablets (traditional Chinese medicines) in water and gastrointestinal fluids by HPLC-ICP-MS, so as to quantitatively evaluate the arsenical toxicity of Niuhuang Jiedu Tablets. METHODS: An HPLC-ICP-MS method for the determination of AsIII, AsV, MMA, DMA and the identification of AsB and AsC was established, and the arsenic speciation of Niuhuang Jiedu Tablets in water, gastric fluid, and intestinal fluid was determined and analyzed. RESULTS: The method validation indicated that the correlation coefficients (r) for As, As, MMA, and DMA were all over 0.9994, the limits of quantitation (LOQ) were between 0.66 and 1.19 μg · L-1, the precisions and stabilities were satisfactory with all RS-Ds less than 5%, and the spiked recoveries ranged from 92.78% to 100.2%. The results of samples analysis showed that only inorganic arsenic (As and As) and trace amount of unknown arsenic speciation were detected, while MMA, DMA, AsB, and AsC were not found in any cases; highly toxic inorganic arsenic (As and As) were dominating arsenic speciation of Niuhuang Jiedu Tablets in water and gastrointestinal fluids. CONCLUSION: This method could be used for the arsenic speciation analysis of Niuhuang Jiedu Tablets in water and gastrointestinal fluids.

OBJECTIVE: To establish a method for rapid determination of chromium (Cr) in medicinal capsules by inductively coupled plasma mass spectrometry (ICP-MS). METHODS: With 45Sc as internal st andard element, Cr in medicinal capsules was directly analyzed by ICP-MS after microwave digestion, and this method was compared with the ChP 2010 method (GFAAS method). RESULTS: The correlative coefficient (r) for Cr was 1.000 O, the limit of detection was 0.079 μg · L-1, the precision and stability were satisfactory with all RSDs less than 4%, the spiked recoveries of high, middle and low level ranged from 96.77% to 101.5%, the determined values of tea reference material (GBW 07605) and tomato leaves reference material (SRM 1573a) met well with those certified. Method comparison indicated good accordance between this method and the ChP method. CONCLUSION: This method is simple and accurate, and could be used as an alternative method to the ChP method.