Endometrial Neoplasms ; classification ; epidemiology ; mortality ; pathology ; Female ; Humans ; Uterine Cervical Neoplasms ; classification ; epidemiology ; mortality ; pathology

Objective To investigate the effects of Rehmannia glutinosa polysaccha-rides (RPS) on myocardial apoptosis of rats after myocardial ischemia-reperfusion injury. Methods The experimental rats were randomly devided into sham operation group, model group, RPS low, medium and high-dose group, Danshen tablets group, 20 rats in each group. All the rats were performed the coronary occlusion operation except the sham operation group. And then, the rats in RPS low, medium and high-dose groups were given RPS 10, 20, 40 mg/kg by intragastric administration;the rats in Danshen tablets group were given Danshen tablets suspension 260 mg/kg;the rats in sham operation group and model group were given the equal volume of saline;once a day for 4 weeks. The areas of myocardial infarction were analysised by TTC staining;the apoptosis of cardiomyocytes was observed by TUNEL and the apoptosis index(AI) were calculated;the expression of bcl-2, Bax, caspase-3 were detected by IHC and were semi-quantitative analysised. Results Compared with the model group, the areas of myocardial infarction (31.7% ± 6.1%, 22.8% ± 5.5% vs. 43.9% ± 6.7%) and the apoptosis index (44.6%± 6.7%, 32.8%± 5.4%vs. 59.4%± 9.0%) in RPS medium and high-dose group (RPS 20, 40 mg/kg) significantly decreased (P<0.05 or P<0.01). The expression of bcl-2 (0.40 ± 0.09, 0.45 ± 0.08 vs. 0.26 ± 0.05) significantly increased (P<0.05 or P<0.01), the expression of Bax (0.55 ± 0.11, 0.28 ± 0.08 vs. 0.85 ± 0.13) and caspase-3 (0.11 ± 0.03, 0.08 ± 0.02 vs. 0.16 ± 0.03) significantly decreased (P<0.05 or P<0.01), and the ratio of bcl-2/Bax (0.73 ± 0.05, 1.61 ± 0.20 vs. 0.31 ± 0.08) significantly increased (P<0.05 or P<0.01). Conclusions RPS had inhibition effects on myocardial apoptosis after myocardial ischemia-reperfusion injury in rats, which may be related to its effects of enhancing the ratio of bcl-2/Bax and down regulating the expression of caspase-3.

Objective:To study the fluorescent manifestations of fundus oculi lesion in several types of retinal degeneration.Methods:Fundus fluorescein angiography(FFA) were performed in 8 patients(16eyes)of retinal degeneration.Results:Lesion of eyes was actinomorphic,and was relatively quiet,grade was low,mainly presented as retinal pigment epithelium(RPE) and choroidal capillary atrophy.Background image choroidal at early stage showed hypofluorescence,and big choroid blood vessel low fluorescence were seen.This differed from secondary retinitis pigmentosa.Conclusion:Results of FFA with retinal degeneration show barrier of choroidal capillary atrophy may be one factor for RPE and neurosensory retina dystrophy.

Objective:To examine the effects of glucose on the expression of matrix metalloproteinases-2(MMP-2)and the changes of ultramicrostructure,cell cycle in cultured human umbilical vein endothelial cells.Methods:Human umbilical vein endothelial cells(hUVEC)were incubated in a culture medium with 11.2mmol/L,16.8mmol/L and 33.6mmol/L glucose concentrations for 24h,48h,72h respectively.The expression of MMP-2 was measured by RT-PCR and Western-blot.Results:When D-glucose concentrations rose,hUVEC exposed to high glucose concentration(11.2mmol/L,16.8mmol/L and 33.6mmol/L)increased in cell expression of MMP-2 after 48 h and 72 h of exposure,as compared with those cultured in medium with a low glucose concentration(5.6mmol/L).And cell cycle changed too.Conclusion:High concentration of glucose can arrest the proliferative response and show that even a short-term exposure of endothelial cells(ECs)to high glucose concentration leads to their activation associated with increased expression of MMP-2 and that this effect may appear more significantly along with time of exposure to high concentration of glucose.

Objective:To assess the clinical setting of a heavy silicone oil tamponade(Densiron-68) for rhegmatogenous retinal detachment(RRD).Methods:Twenty-nine eyes of 29 consecutive patients were recruited between December 2007 and February 2009.Primary vitrectomy with Densiron-68 was used in all cases.Patients to be selected had two groups:one group had 18 cases,mean age was 72.5 years.These patients were impossible prone because of ages and body factors.Among these of 11 cases had macular holes and posterior pole part holes,another of 7 cases had many holes.Another group had 11 cases,mean age was 45.8 years.These patients with inferior complex rhegmatogenous retinal detachment with secondary proliferative vitreoretinopathy(PVR) were included.Besides one case of giant retinal tear with between 2 and 7 clock hours,among these of 7 cases had one or two scleral buckling procedure,or pars plana vitrectomy with oil endotamponade.But inferior recurrent retinal re-detachment appeared.Surgical techniques included pars plana vitrectomy,membrane peeling,heavy water application,endophotocoagulation,cryocoagulation,endotamponade et al.Results:1or 13-month follow up,mean 4.3-month.Densiron-68 removal with 25 cases was after operation of 2 months,and didn't remove of 4 cases.After Densiron-68 removal,majority showed a stable reattached retina without further interventions,while,in one or two patients,recurrent retinal re-detachment appeared during Densiron-68 removal.Some of patients had partly lens opacification or lens post-capsular opacification.Part of patients had ocular hypertension.Conclusion:With Densiron-68,high anatomical and functional success rates can be achieved with inferior complex rhegmatogenous retinal detachment.Heavy silicone oil tamponade improves inferior tamponade,and may be considered a new generation of intraocular tamponades.But there were a significant early emulsification.Despite adequate Densiron fills,emulsification necessitated its removal.

? AIM: To evaluate efficacy of Ranibizumab and Conbercept for wet age-related macular degeneration ( wAMD) .?METHODS: This was a retrospective case series study. Thirty patients ( 30 eyes ) with wAMD were enrolled to receive intravitreal injections of ranibizumab (0.5mg) on 3 consecutive monthly schedule and 28 patients (30 eyes) with wAMD were enrolled to receive intravitreal injections of conbercept ( 0.5mg ) on 3 consecutive monthly schedule.Best corrected visual acuity ( BCVA ), optic coherence tomography ( OCT ) measurement were compared at 1mo after injections.?RESULTS: One month after every injection, the BCVA increased while the central macular thickness ( CMT ) decreased compared with those before treatment in group A and B (P<0.05).BCVA and CMT changes between two groups were no statistically different (P>0.05).?CONCLUSION:Ranibizumab and conbercept therapy can control the prognosis of wAMD and improve the vision effectively. There is no statistical difference on the curative effect between two drugs for 3mo.

Wet age-related macular degeneration is the leading cause of the severe, irreversible vision loss in individuals over the age of 65 years. Anti-VEGF therapy has been shown to play a key role in the pathogenesis of wAMD, which is the front-line therapy admittedly. lt has no clear steady curative effect for some patients even if they accepted repeating Anti-VEGF therapy. For the purpose of visual acuity improved more, a steady flow of new therapy has emerged, such as function towards the same or different targets of antiangiogenesis, consolidating the effect by combination therapy, improving or simplifying the mode of administration, etc. This paper gives a brief review of the progress of anti-VEGF for the therapy in wet age-related macular degeneration.

Objective To study the relationship between serum lactadherin (MFG-E8),sialic acid (SA) level and coronary heart disease (CHD) occurrence and development.Methods 166 patients in our hospital were selected from June 2014 to November 2015,and then according to the result of coronary angiography divide the subjects into control group (n=56,the result of coronary angiography completely normal) and CHD group,including stable angina 48 cases (StA subgroup),unstable angina 30 cases (UA subgroup) and acute myocardial infarction 32 cases (AMI subgroup).The serum level of MFG-E8 was detected by ELISA method,the serum level of SA,hs-CRP and lipid index were detected by biochemical analysis.Gensini score showed the severity of coronary stenosis.The correlation analysis between detecting parameters was carried out by Spearman method.Results Compared with the control group,the patients with CHD have a higher level of serum SA and a lower level of MFG-E8 (both P<0.05).The AMI subgroup has a lower level of serum MFG-E8 than StA and UA subgroups,and the AMI subgroup has a higher level of serum SA than StA and UA subgroups (all P<0.05).The correlation analysis showed that the serum SA has a positively correlation with the level of TG,hs-CRP,and Gensini score,and has a negatively correlated with the level of MFG-E8 (all P<0.05).The MFG-E8 has a negatively correlated with the level of TG,hs-CRP,and Gensini score (all P<0.05).Conclusion MFG-E8 and SA play an important role in the development of CHD,and combined detection of serum MFG-E8 and SA may be helpful in the clinical diagnosis and risk assessment of patients with CHD.

Objective:To explore the relationship between coronary slow flow (CSF) and vascular inflammatory fac-tors .Methods :A total of 229 inpatients with normal coronary angiography (CAG) results were selected as research object .According to CAG TIMI flow and corrected TIMI frame count ,patients were divided into CSF group (n=102 ,no obvious lesion in epicardial coronary artery but coronary flow was slow ) and normal coronary group (n=127 ,completely normal epicardial coronary artery and blood flow ) .General clinical data were collected ,vascular inflammatory factors were measured and their relationship with CSF was analyzed .Results:Compared with normal coronary group ,there were significant rise in levels of high sensitive C reactive protein [ (1.41+0.72 ) mg/L vs . (2.82+0.58) mg/L] ,interleukin 1 [ (0.18 ± 0.05)μg/L vs .(0.26 ± 0.06)μg/L] and interleukin 6 [ (0.363 ± 0.016) ng/L vs .(0.465 ± 0.015) ng/L] in CSF group , P<0.05 all .Logistic regression analysis indicated that vas-cular inflammatory factors were risk factor for CSF occurrence (OR= 1.008 , P= 0.015 ) .Conclusion:Coronary flow velocity is closely related to vascular inflammatory factors .

Objective:To explore the therapeutic effect of nicorandil based on routine medication on stable coronary heart disease (CHD) .Methods :A total of 100 inpatients diagnosed as stable CHD were enrolled ,randomly and e-qually divided into nicorandil group (received nicorandil 5mg based on routine medication ,three times/d) and rou-tine treatment group .After discharge ,patients were followed up for six months .Angina pectoris frequency ,ECG , serum level of high sensitive C reactive protein (hsCRP) and 6min walking distance (6MWD) were compared be-tween two groups before and after follow up .Results:After six-month follow up ,compared with routine treatment group ,there were significant improvements in clinic therapeutic effect (48% vs .74% ) and ECG therapeutic effect (78% vs .96% ) in nicorandil group , P<0.05 all;compared with before follow-up ,there were significant reduc-tions in angina pectoris frequency and serum hsCRP level ;and significant rise in 6MWD in both groups , P<0.05 all;compared with routine treatment group ,there were significant reductions in angina pectoris frequency [ (10.35 ± 1.51) times/week vs .(9.95 ± 1.65) times/week] and hsCRP level [ (1.12 ± 0.51) mg/L vs .(0.95 ± 0.43) mg/L];and significant increase in 6MWD [ (342.38 ± 35.64) m vs .(388.64 ± 32.43) m] in nicorandil group , P<0.05 all .Conclusion:Nicorandil can effectively reduce the attack number of angina pectoris of stable coronary heart disease and serum hsCRP level ,increase exercise tolerance and improve clinical therapeutic effect .