1.Effects of four novel root-end filling materials on the viability of periodontal ligament fibroblasts.
Makbule Bilge AKBULUT ; Pembegul Uyar ARPACI ; Ayce Unverdi ELDENIZ
Restorative Dentistry & Endodontics 2018;43(3):e24-
OBJECTIVES: The aim of this in vitro study was to evaluate the biocompatibility of newly proposed root-end filling materials, Biodentine, Micro-Mega mineral trioxide aggregate (MM-MTA), polymethylmethacrylate (PMMA) bone cement, and Smart Dentin Replacement (SDR), in comparison with contemporary root-end filling materials, intermediate restorative material (IRM), Dyract compomer, ProRoot MTA (PMTA), and Vitrebond, using human periodontal ligament (hPDL) fibroblasts. MATERIALS AND METHODS: Ten discs from each material were fabricated in sterile Teflon molds and 24-hour eluates were obtained from each root-end filling material in cell culture media after 1- or 3-day setting. hPDL fibroblasts were plated at a density of 5 × 103/well, and were incubated for 24 hours with 1:1, 1:2, 1:4, and 1:8 dilutions of eluates. Cell viability was evaluated by XTT assay. Data was statistically analysed. Apoptotic/necrotic activity of PDL cells exposed to material eluates was established by flow cytometry. RESULTS: The Vitrebond and IRM were significantly more cytotoxic than the other root-end filling materials (p < 0.05). Those cells exposed to the Biodentine and Dyract compomer eluates showed the highest survival rates (p < 0.05), while the PMTA, MM-MTA, SDR, and PMMA groups exhibited similar cell viabilities. Three-day samples were more cytotoxic than 1-day samples (p < 0.05). Eluates from the cements at 1:1 dilution were significantly more cytotoxic (p < 0.05). Vitrebond induced cell necrosis as indicated by flow cytometry. CONCLUSIONS: This in vitro study demonstrated that Biodentine and Compomer were more biocompatible than the other root-end filling materials. Vitrebond eluate caused necrotic cell death.
Apicoectomy
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Apoptosis
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Cell Culture Techniques
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Cell Death
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Cell Survival
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Dentin
;
Endodontics
;
Fibroblasts*
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Flow Cytometry
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Fungi
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Humans
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In Vitro Techniques
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Miners
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Necrosis
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Pemetrexed
;
Periodontal Ligament*
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Polymethyl Methacrylate
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Polytetrafluoroethylene
;
Survival Rate