Objective To observe the change of glucocxnticoid(GC) and the messenger RNA expression of its receptor(GRmRNA) in MRL/lpr mice with the intervention of nourishing yin and clearing heat decoction.Methods Forty MRL/lpr mice were randomly divided into four groups:model control group,TCM group,west medicine group,combined treatment of traditional chinese medicine and west medicine group,10 mice in each group.The group was intragastrically administrated normal sodium,nourishing yin and clearing heat decoction,prednisone suspension,nourishing yin and clearing heat decoction plus prednisone suspension,0.5 mL every time,once daily for 10 weeks respectively.Meanwhile,10 Kungming mice were selected as normal group,administrated normal sodium,like the model group.The expression of glucocxnticoid receptor-alpha and beta(GR?mRNA,GR?mRNA) were detected by reverse transcription polymerase chain reaction(RT-PCR).Results Compared with normal,the expression of GRmRNA was increased in model groups,but the level of GC was not different.Compared with model group,GR?mRNA and GR?mRNA of MRL/lpr mice were improved in all treatment groups,especially in combined treatment of traditional Chinese medicine and western medicine group.Conclusion Nourishing yin and clearing heat decoction has effect of upregulating the expression of GRmRNA in MRL/lpr mice,so as to enhance the effect of glucocorticoid and reduce its side effects.

OBJECTIVE:To evaluate the effect of CD44 on drug resistance of breast cancer patients. METHODS:62 breast cancer patients were selected,and the expression of CD44 was detected by immunohistochemical method. Drug resistance was eval-uated by WHO criteria,and the expression of CD44 in breast tissue was observed after observation. RESULTS:There were 48 pa-tients sensitive to CD44 and 14 patients with drug resistance,with statistical significance(P<0.05). After chemotherapy,the ex-pression of CD44 in caner tissue was higher than before chemotherapy,that of patients with drug resistance was higher than pa-tients sensitive to CD44,with statistical significance(P<0.01). CONCLUSIONS:CD44 antigen is closely related to chemothera-py resistance of breast cancer,and high expression of CD44 can be used as an important indicator for breast cancer chemotherapy.

Objective To investigate anti-ulcerative drug use in patients undergoing arthroscopic operation against stress ulcer. Methosds A total of 297 patients undergoing arthroscopic operation during Jan. to Aug. 2013 in our hospital were recruited and retrospectively analyzed with Excellspreadsheet. Results Among 297 patients in the study, ninety-four patients (31.6%) used AUD before operation, most (94.9%) administered by intravenous, and drug utilization index was 1.35. Ninety-eight point nine patients used proton pump inhibitors (PPIs) in which Lansoprazole was prescribed most. Conclusion This study highlights the overuse of PPIs in patients undergoing arthroscopic operation.