Objective To assess the application value of serum procalcitonin(PCT)for the differentiation between the neonates with or without septicemia.Methods PCT,C-reactive protein(CRP),blood and blood culture tests were done in 75 cases of neonatal septicemia as the therapy group,and 74 neonates without the signs of infection as the control group after the admission of our hospital One week later,the therapy group also re-examined the PTC and CRP tests.Furthermore,the results were analyzed.Results The levels of CRP(23.26±5.68)mg/L and PCT(7.61±4.53)ng/L in therapy group were dramatically reduced compared with(4.09±1.09)mg/L,(1.95±0.25)ng/L in therapy group,and(6.42±4.54)mg/L,(0.45±0.16)ng/L in the control group before therapy,the t values are 28.71,12.97 and 19.97,14.08 respectively,and p value are both less than 0.01.The positive rates of PTC,CRP and blood culture were 82.7％,48.0％ 28.0％ before the admition to hospital.The differences of PTC and CRP,PTC and blood culture had statistical significances(x2=5.64,P＜0.05;x2=10.40,P＜0.01).If 2.5 μg/L of PCT and 8mg/L of CRP would be regarded as cutoff point in our study,the sensitivity and specificity of PCT or CRP for diagnosis of neonatal septicemia was 82.7％ and 80.3％,48.7％ and 78.4％.Conclusion Our data suggested that the serum PTC is a valuable marker for the early diagnosis of neonatal septicemia.

OBJECTIVE To conduct the centralized management of the medical instrument in the supply room,and to assure the quality and validation of the clinical nursing and the patient safety. METHODS The cleaning and packing procedures of the instrument were standardized.The reusable instrument would be concentrated to clean,pack,and sterilize.To create a photograph management system,and use the photos to distinguish specialized instrument. RESULTS The instrument was reprocessed in the supply room instead of the clinical departments by the method of the centralized management of the instrument,which provided better instrument care and reprocessing. CONCLUSIONS The supply room conducts the centralized management of the medical instrument and enhances the quality of the instrument reprocessing.

Objective To investigate the predisposing alleles of HLA-DR genes in pemphigus. Methods Polymerase chain reaction specific sequence primers (PCR-SSP) method was applied to type HLA-DR subregion in the patients with pemphigus vulgaris (PV), pemphigus erythematosus (PE) and matched control subjects of Han nationality from Jiangsu and Anhui provinces. Results The results demonstrated that DR4, DRB1*14 (*1401, *1404,*1405)gene frequencies were significantly higher in both PV(Pc

Objective To explore the value of serum CA125 in prediction of the first-trimester threatened abortion.Methods The serum CA125 level of 78 patients with threatened abortionand( miscarriage success group and failure group) and 40 normal early pregnant women before treatment and after 1 ～ 4 weeks were tested by chemiluminescent immunoassay(CLIA) methods and the results were compared.Results The serum CA125 level of the failure group was significantly higher than that of the miscarriage success group[ (28.52 ± 19.12) x 103 U/L] and normal early pregnant women group [ (20.45 ± 9.55) × 103 U/L] ( t =- 1.28,- 1.24,all P ＜ 0.05 ),and it increased by degree and by time,and the sensitivity and specificity of detection was 93.1％ and 87.85％ respectively.Conclusion Monitoring of serum CA125 had highly clinical value in predicting the prognosis of threatened abortion.

A 36-year-old female presented with multiple dark brown papules on the chest and abdomen for more than 10 years,which had gradually increased in number.Physical examination revealed dozens of dark brown,flat papules measuring 1-3 mm in diameter in the chest and abdomen.Most of the lesions had a smooth surface,and some lesions gave a papilloma-like appearance,with no confluent trend.Biopsy of abdominal lesions showed mild hyperkeratosis of epidermis,acanthosis,extension of epidermal protrusions forming a reticulated appearance,horn pseudocysts in prickle cell layer,enhanced pigmentation of basal layer,and a sparse lymphocytic perivascular infiltrate in superficial dermis.A diagnosis of dermatosis papulosa nigra (DPN) was made.

The effects of Artemether on immune responses were investigated in mice. After p.o. administration of Artemether 200 mg/kg?d-1 for 4-7 days, it was found that: ( 1 ) the percentage of phagocytosis in peritoneal macrophage was reduced, but the phagocytic index was not significantly affected; ( 2 ) the level of serum IgG was markedly lowered in the mice sensitized by cock red blood cell ( CRBC ); ( 3 ) the hemolysin-forming capacity of CRBC-sensitized mice was significantly enhanced; ( 4 ) the weight of thymus was markedly decreased in the mice sensitized with CRBC; ( 5 ) no significant effect in PHA-induced lymphocyte transformation was found.

Attenuation reflectance Fourier transformation infrared spectrometry(ATR FTIRs) combined with clustering analysis was used to identify genus Zanthoxlum .The dendrogram of clustering analysis showed that there were obvious difference between genus different species of Zanthoxlum . The results are consistent with that of morphologic study and analyzing.This method is rapid, simple, effective and can be used for the identification of crude drugs.

Objective To detect the mRNA and protein expressions of desmoglein 1 (DSG1) and DSG3 in different types of keratinocytes (KCs).Methods Two keratinocyte cell lines HaCaT and A431,as well as primary keratinocytes from human abdomenal skin served as the object of this study.Direct immunofluorescence assay was performed to observe and quantify the expressions of DSG1 and DSG3,and quantitative PCR (qPCR) to determine the mRNA expressions of DSG1 and DSG3,in these cells.Results Both DSG1 and DSG3 were expressed in all the three types of keratinocytes,and the fluorescence intensity of DSG1 and DSG3 in HaCaT cells was higher than that in primary keratinocytes but lower than that in A431 cells.Similarly,all the keratinocytes expressed DSG1 and DSG3 mRNA,with the relative expression levels of DSG1 and DSG3 mRNA in primary keratinocytes being 291.7％ and 237.4％ of those in HaCaT cells respectively (both P ＜ 0.01),and those in A431 cells being 0.1％ and 18.8％ of those in HaCaT cells respectively (both P ＜ 0.05).Conclusions HaCaT cells,A431 cells and primary keratinocytes all can be used for the study of DSG1 and DSG3,of which,A431 cells show the strongest expressions of DSG1 and DSG3,and primary keratinocytes display the highest expressions of DSG1 and DSG3 mRNAs.

Objective To explore the expression of neutrophil adhesion molecule CD11b,and plasma level of elastase in patients with anaphylactoid purpura during different clinical phases and their correlation with disease activity.Methods A total of 20 patients with anaphylactoid purpura were recruited into this study,along with 20 normal human controls.Two blood samples were collected from each patients at the first visit(active phase)and after 3～5 weeks of treatment(remission phase).The expression of CD11b was measured by flow cytometry in 12 patients and normal controls,and plasma levels of elastase by ELISA in 20 patients and normal controls.Results Increased CD11b expression and elastase level were noted in patients in active phase compared with those in patients in remission phase(3367.25±434.57 vs 2569.33±411.06.13.98±2.05 vs 4.29±0.80.both P＜0.01).No significant difference was found in CD11b expression between patients in remission phase and normal controls(P＞0.05).while the elastase level was higher in patients in remission phase than in normal controls(4.29±0.80 vs 3.67±0.54.P＜0.05).In active phase of anaphylactoid purpura,the expression of CD11b was positively correlated with the plasma level of elastase(r=0.73,P＜0.01),while no correlation was noticed between them in remission phase(r=0.20,P=0.54).Conclusion Peripheral neutrophils are activated in anaphylactoid purpura,which seems to be more obvious in active phase than in remission phase.

Objective To investigate the mRNA expression of Fc gamma RⅡA(FcγR ⅡA)on polymorphonuclear neutrophils(PMN)from patients with Beh(c)et's disease(BD).Methods Twenty-five patients with active BD and 20 healthy human controls were included in this study.Blood samples were obtained from all patients with active BD before treatment,from 15 patients with inactive BD after treatment and from healthy controls.PMN were isolated.The FcγR Ⅱ A mRNA expression on PMNs was detected by RT-PCR,and plasma myeloperoxidase (MPO)activity which represented neutrophil activation,was measured spectrophotometricaily.Results The relative expression level of FcγR Ⅱ A on PMN and plasma MPO aetivity were 1.80 1±0.829 and 32±5 U/L.respectively,in patients with active BD,0.820±0.625 and 27±4 U/L,respectively,in those with inactive BD,and 0.745 ±0.931 and 29±5 U/L,respectively,in normal controls;the differences were significant in the two parameters between the patients with active and inactive BD (both P＜0.01),while no statistical difference was observed between inactive patients and normal human controls(P＞0.05).There was a positive eorrelation between the expression level of FcγR Ⅱ A on PMN and plasma MPO activity in patients with BD(r=0.39,P＜0.0 1).Conclusions The mRNA expression of FcγR ⅡA on neutrophils is up-regulated in patients with active BD.It is likely that FcγR Ⅱ A is involved in the activation of neutrophils in BD.