Objective:To evaluate the quantity and function changes of dendritic cells ( DC) in peripheral blood of patients with repeated implantation failure ( RIF).Methods:30 patients with RIF and 15 normal controls were enrolled in this study,and the peripheral blood was collected during the mid-luteal phase.The percentage of DC subsets and the expression levels of functional molecules were assessed by flow cytometric analysis.Results:Compared with normal controls,the percentage of lin-HLA-DR+DC cells accounting for leukocytes in patients with RIF was not significantly different ( P>0.05).There were also no significant differences in the expression levels of co-stimulatory molecules ( CD80 and CD86) and immune tolerant molecules CD200 on DC cells surfaces between patients with RIF and normal controls ( all P>0.05).In addition,the percentage of CD11c+CD123-mDC accounting for DC cells was significantly increased in patients with RIF (P<0.05),however,the percentage of CD11c-CD123+pDC was similar (P>0.05). Conclusion:The percentage of mDC accounting for DC cells was significantly increased in patients with RIF, which may be one of factors affecting pregnancy outcomes.

Objective To assess the influence of overall nursing intervention and lumbar and abdominal muscle strength training on the lumbar vertebral function of patients with lumbar intervertebral disc protrusion. Methods Seventy-six patients with lumbar intervertebral disc protrusion treated in our hospital from May 2013 to December 2014 were ran-domly divided into the control group and the observation group. The control group was given simple lumbar and ab-dominal muscle strength training, and the observation group was given additional overall nursing intervention on the ba-sis of lumbar and abdominal muscle strength training. The changes of lumbar vertebral function scores and the patient satisfaction rates of the two groups were compared. Results Before nursing training, the two groups were not signifi-cantly different in the lumbar vertebral function score (P>0.05); after nursing training, the observation group was sig-nificantly higher than the control group in the lumbar vertebral function score, with statistically significant difference(P<0.05). The observation group was significantly higher than the control group in the patient satisfaction rate, with statistically significant difference between the two groups (P<0.05). Conclusion Overall nursing intervention and lum-bar and abdominal muscle strength training can effectively improve the patients' awareness of disease,gain their treat-ment compliance and show remarkable effects and high patient satisfaction rate,thereby worthy of clinical promotion.