Objective To analyze the relationship between hepatitis B virus gene mutation at site 1896 in precore region and genotypes as well as liver function parameters. Methods The fluorescent quantitative PCR and sequencing method were applied to measuring the relevant indicators in 50 patients with chronic hepatitis B. Results There was significant difference in ALT level between hepatitis B patients with site 1896 mutation and ones with wild-type; and HBV mutation at site 1896 in precore region was unrelated to the genotypes. Conclusion HBV mutation at site 1896 in precore region may be associated with continous viral invasion invasion into hepatocytes.

Object To determine the content of glycyrrihizic acid obtained when each individual ingredient in MAHUANG DECOCTION was decocted separately and then mixing the extracts with boiling water in comparison with that obtained by decocting the total composition together as a whole in the traditional way. Methods The contents of glycyrrhizic acid was determined by HPLC. Hypersil C 18 column was used, with acetonitrile∶0.1% acetic acid (33∶67) as the mobile phase and detected at the wavelength of 254 nm. Results The average recovery of glycyrrhizic acid when separately decocted was 102.43%, RSD=2.65%, while that of decoction in whole was 99.41%, RSD=3.11%. Conclusion The method was simple and accurate and was not interfered by other constituents in the prescription.

Objective: To compare the contents of ferulic acid in single medicine decoction (preparing the refined granules of single traditional Chinese herb from the different kinds of medicinal materials separately then mixing the granules with boiling water) and mixed medicine decoction (decocting the all kinds of the medicinal materials together as the traditional method) of Tangkuei Blood Supplementing Decoction (composed of Radix Angelicae Sinensis and Radix Astragali). Methods: HPLC conditions: Hypersil C 18 column methanol -0.05% acetic acid solution (45∶55) as mobile phases, detection wavelength at 324 nm. Results: The average recovery of single medicine decoction was 100.86% with RSD =2.33%( n =6), and that of mixed medicine decoction was 101.37% with RSD =1.52%( n =6).Conclusion: The content of ferulic acid mixed medicine decoction was higher than that in single medicine decoction.

Object To compare the contents of chlorogenic acid in the different processed crude drugs of Lonicera fulvotomentosa Hsu et S.C. Cheng. Methods The contents of chlorogenic acid were deter mined by HPLC. Chromatographic conditions included Hypersil C 18 column and the mobile phase consisting of a mixture of acetonitrile 0 4% phosphoric acid (13∶87). Chlorogenic acid was detected at 327 nm wavelength. Results The contents of chlorogenic acid were 0 54%, 0 81%, 0 57% in the sun cured flower bud, white bloom, gold flower in L. fulvotomentosa and 2 14%, 1 65%, 2 04% in the steaming sun cured crude drugs, respectively. Conclusion The contents of chlorogenic acid in the steaming sun cured crude drugs are higher than those in the sun cured crude drugs.

From June 2015 to June 2020, 52 patients who underwent emergency gastroscopy and treatment for upper gastrointestinal bleeding after percutaneous coronary intervention (PCI) at the Renmin Hospital of Wuhan University were included and retrospectively analyzed. The etiologic diagnosis of bleeding, results of hemostatic treatment and occurrence of complications during the emergency gastroscopy were observed. Among the 52 patients, 47 cases (90.4%) were found to have lesions that could explain upper gastrointestinal bleeding under emergency gastroscopy, and the remaining 5 cases (9.6%) could not clearly diagnose the cause of bleeding. Twenty-six cases (50.0%) were treated with emergency endoscopic hemostasis, of which 25 cases (96.2%) were successful. The median time for emergency endoscopy was 18 min. Seven cases (13.5%) had transient slowing of heart rate and lowering of blood pressure without serious complications. This shows that emergency gastroscopy is safe and effective in the diagnosis and treatment of upper gastrointestinal bleeding combined with post-PCI.