Objective To investigate the effect of Lecarnidipine on hypertension and circulation hematopoietic progenitor cells (HPCs) count in elderly patients.Methods A total of 61 elderly patients with hypertension were selected in Renji Hospital geriatric hypertension clinic from January 2014 to August 2014.Patients were randomly divided into two groups:Lercanidipine treatment group (n=32,Lercanidipine hydrochloride 5-10 mg/day),the control group (n=29,thiazide diuretics and / or beta blockers according to patient's condition).Patients were observed for 12 weeks,and the target blood pressure level was ＜140/90 mmHg (1 mmHg=0.133 kPa).Blood lipids and glucose levels,liver and kidney function and erythrocyte sedimentation rate (ESR) were determined before and after treatment.The quantity of circulation HPCs (the percentage of CD34+ CD45dim in peripheral blood in 100000 mononuclear cells) was detected by flow cytometry analysis.Results Blood pressure in Lercanidipine treatment group was decreased after the treatment (P＜0.01),and patients reached the target blood pressure.There was no difference in the decrease of blood pressure level between the two groups (P＞0.05).The number of CD34+ CD45dim cells was increased after 12 weeks of Lecarnidipine treatment [(0.022 + 0.003)％ vs.(0.034 + 0.028)％,P＜0.05].Compared with the control group,the number of CD34+ CD45dim cells in lercanidipine group had a rising trend,but it had no statistical difference (P＞0.05).Taking the number of CD34+ CD45dim as the independent variables,the multiple stepwise linear regression analysis showed that the number of CD34+ CD45dim cells was negatively correlated to systolic pressure.Conclusions The number of circulation HPCs is negatively correlated with systolic pressure in the elderly.Lercanidipine can increase the number of circulation HPCs,and does not rely on its antihypertensive effect.

Objective To investigate influential factors and incidence of postpartum urinary retention after forceps delivery. Method The clinical data of 216 cases of puerperas undergoing forceps delivery in our hospital from January 2012 and July 2015 were collected and investigated in terms of postpartum urinary retention as well as its influencing factors. Results The incidence rate of postpartum urinary retention was 24.07%(52/216).Logistic multivariate regression analysis showed the significant positive variables for the outcomes included:the first stage of labor and postpartum 2h bleeding.Conclusion The first stage of labor time and postpartum 2h bleeding are the high risk factors of uroschesis after forceps delivery.

Objective To explore the prognostic role of laminin (LN ) as a tumor biological marker in predicting the recurrence of hepatocellular carcinoma (HCC) related with HBV infection after liver transplantation (LT ) .Methods Tissue samples from 251 HBV-related HCC patients undergoing LT were immunohistochemically stained with anti-LN antibody .The relevant prognostic factors were analyzed using Spearman's rank test , Kaplan-Meier method , log-rank test and multivariate step-wise Cox regression analysis .Results The expressions of LN in tumor tissues were significantly positively correlated with tumor number (P=0 .00) ,microsatellite (P=0 .02) ,venous invasion (P=0 .048) ,pTNM tumor stage (P=0 .00) ,pre-LT serum α-fetoprotein (AFP) level (P=0 .00) ,HBV DNA level (P= 0 .02) ,HBeAg level (P= 0 .02) and tumor recurrence (P= 0 .00) respectively .Significant differences existed in 1/3/5-year overall survival or tumor recurrence-free survival rate post-LT among LN different expression (-,+ ,≥ + + ) in HBV-related HCC patients (P< 0 .05 ) . Multivariate analysis indicated that LN was a significantly independent predictor in predicting poor tumor recurrence-free survival post-LT (P=0 .01) .Conclusions LN may be a feasible marker in predicting HCC recurrence post-LT for HBV-related HCC patients .

Objective: To study the overexpression of protein phosphatase 2 regulatory subunit B''α gene effects on the proliferation and invasion of hepatoma cells. Methods: Immunohistochemistry method was used to analyze the expression of PPP2R3A in cancerous and paracancerous tissues. Hepatocellular carcinoma cell lines (Huh-7 and HepG2) with stably overexpressing PPP2R3A were constructed by lentiviral vector. Biological behavioral transition in hepatocellular carcinoma cell proliferation, cell cycle, apoptosis, invasion and metastasis were detected by cell counting kit-8 assay (CCK-8), flow cytometry, and transwell assay. A subcutaneous nude tumor mice model was constructed to validate the growth of hepatoma cells. Two independent sample t-tests were used to compare the groups. Results: The expression of PPP2R3A gene in human hepatocarcinoma tissues was higher than paracancerous tissues. The absorbance (A value) of hepatoma cells was increased (P < 0.05) after overexpression of PPP2R3A gene. The transition from G1-to-S phase was significantly increased i.e., the G1 phase of the cell cycle was reduced (Huh-7: t = 3.04, P = 0.0384; HepG2: t = 4.06, P = 0.0153), while the S phase was increased (Huh-7: t = 3.47, P = 0.0255; HepG2: t = 4.46, P = 0.0112). Early apoptotic rate was decreased (Huh-7: t = 7.34, P = 0.0018; HepG2: t = 4.06, P = 0.0153). The number of Huh-7 cells migrating to the lower chamber was increased (t = 3.18, P = 0.0334), and after the use of matrigel the number of cells reaching to the lower chamber was also increased (t = 2.84, P = 0.0464). The results of animal experiments showed that the subcutaneous tumor growth (t = 4.31, P = 0.0035) was significantly overexpressed in nude mice group. The results of Western blot showed that the expression of PARP and P53 protein in the spliced ​​forms decreased, while the accumulation of β-catenin protein in the liver cancer cells was increased. Conclusion Overexpressed PPP2R3A gene may promote proliferation, migration and invasion ability, inhibit apoptosis, induce G1/S phase transition, and participate in the biological behavior of hepatoma cells.