Objective To investigate the roles of TrkA and TrkB in radiation-induced hippocampal neurogenesis impairment.Methods Fifty-six rats were randomized into radiation group and sham control group.Radiation group received whole brain irradiation at a single dose of 10 Gy.The hippocampus were separated from rats in day 1,day 3,day 14 and 1 month after irradiation.Western blot and RT-PCR were applied to detect the protein levels and mRNA levels.Golgi staining was used to observe the dendritic spine of hippocampus.Immunofluorescence was performed to detect neural precursor's proliferation.Results Compared with control group,the numbers of dendritic spine significantly decreased after irradiation and its shape change obviously.Immunofluorescence showed a significant decrease in neural precursor's proliferation comparing with control group (t =6.49,P ＜ 0.05).Protein level of TrkA expression increased (t =2.64,3.06,4.80,2.64,P ＜ 0.05),while the levels of TrkB protein expression decreased significantly (t =4.59,3.06,2.81,2.57,P ＜ 0.05).The mRNA level of TrkA expressions increased (t =4.57,3.06,5.39,5.86,P ＜ 0.05),while the mRNA level of TrkB decreased (t =14.87,11.69,4.98,P ＜ 0.05).Conclusions As a signaling pathways downstream of NGF and BDNF,TrkA and TrkB may play an important role in radiation-induced neurogenesis impairment.

Objective To investigate the characteristics of transient remission of islet function and its influence on long-term prognosis in different onset age patients with type 1 diabetes mellitus(T1DM).Methods 156 newly diagnosed T1DM patients who received treatment and completed follow-up were retrospectively chosen from January 2014 to January 2018.All patients were grouped according to the onset age including childhood group(n=18),adolescent group(n=108),adult group(n=30).The transient remission and retention rate of islet function of three groups were compared.The relationship between onset age and c-peptide level was analyzed.Results BMI,FC-P,and 2 hC-P levels in adolescent group were higher than those in childhood group(P<0.05).The positivity rates of protein tyrosine phosphatase and zinc transporter 8 antibodies in adult group were lower than those in childhood group(P<0.05).The incidence of transient remission of pancreatic function in adolescent group was higher than that in childhood and adult groups(P<0.05).The duration of transient remission of pancreatic function in adolescent and adult groups was lower than that in childhood group(P<0.05).The islet function retention rates in adolescent group after 1 and 4 years from onset were higher than those in childhood and adult groups(P<0.05).Generalized estimation equation analysis showed that the long-term FC-P and 2 hC-P in adolescent group were higher than those in childhood and adult groups(P<0.05).The FC-P and 2 hC-P levels in adult patients with previous transient remission were higher than those in childhood group(P<0.05).Conclusions Adolescent onset T1DM patients are more likely to have transient remission of islet function,while childhood onset patients have longer transient remission of islet function.In addition,the adult T1DM patients had the best long-term islet function in transient remission population.

Objective:To compare the clinical effects of liraglutide and benaglutide in adjuvant treatment of type 2 diabetes mellitus (T2DM) patients complicated with nonalcoholic fatty liver disease (NAFLD).Methods:From January 2017 to January 2019, 110 T2DM patients complicated with NAFLD were chosen in the Fifth People's Hospital of Datong and divided into two groups according to the random digital table method, with 55 patients in each group.On the basis of metformin, the control group was given liraglutide, and the observation group was given benaglutide.The levels of BMI, waist circumference, fasting blood glucose(FPG), postprandial 2h blood glucose(2 h PG), glycosylated hemoglobin(HbA1c), fasting insulin(FINS) release level, insulin resistance index(HOMA-IR), total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), levels of glutamate aminotransferase(ALT), aminotransferase(AST), controlled attenuation parameter(CAP) and liver stiffness(LSM) before and after treatment of the two groups were compared.Results:After treatment, the levels of BMI, waist circumference, FBG, 2 h PG, HbA1c, FINS, HOMA-IR, lipid index, ALT, AST, CAP and LSM of the two groups were significantly better than those before treatment( t=3.17, 3.80; 4.95, 5.66; 3.41, 3.50; 3.85, 5.06; 4.43, 4.60; 3.55, 4.17; 3.91, 4.07; 3.26, 3.59; 4.10, 5.27; 4.01, 4.89; 3.62, 3.20; 3.97, 4.62; 4.01, 5.37; 4.66, 5.13; 4.95, 5.87; all P<0.05). After treatment, the levels of BMI, waist circumference, 2 h PG, ALT, AST, CAP and LSM of the control group were (22.01±0.98)kg/m 2, (1.63±0.38)cm, (7.04±1.71)mmol/L, (39.31±4.92)U/L, (31.40±4.94)U/L, (197.48±36.39)dB/m, (7.42±1.34)kPa, respectively, which were significantly worse than those of the observation group [(19.87±0.76)kg/m 2, (0.89±0.21)cm, (6.29±1.10)mmol/L, (36.17±3.16)U/L, (28.63±3.59)U/L, (152.77±30.18)dB/m, (6.17±1.06)kPa] ( t=2.74, 3.55, 2.81, 3.02, 2.76, 2.89, 3.15, all P<0.05). Conclusion:Compared with liraglutide, benaglutide in adjuvant treatment of T2DM patients complicated with NAFLD can efficiently decrease body weight, improve postprandial blood sugar levels, protect liver function and is helpful to inhibit liver fat deposition.