Malignant lymphoma comes from the lymphoid tissues, which is closely related to immune. The tumor occurs mainly in lymph nodes, but also can occur in the lymph nodes and non-lymphoid tissues. Western medicine treatment for lymphoma is often recurrence after chemotherapy and radiotherapy. TCM not only can play a role in reducing toxicity and increasing the effect of chemotherapy, but also has a good effect on the prevention and treatment of recurrence and metastasis. Professor ZHANG Pei-tong in tumor department of Guang’anmen Hospital of China Academy of Chinese Medicine Sciences usesLiujun Ermu Decotion, which has very good efficacy for malignant lymphoma. This article concluded the clinical experience of Professor ZHANG Pei-tong in usingLiujun ErmuDecotion.

Objective To investigate the Effect of Tetramethylpyrazine on adhesion and invasion ability of Cell PG in Hypoxic Environment. Methods The revival cell PG was divided into three dosages(high, medium and low)group of Tetramethylpyrazine, DDP group and negative control group. All of five groups were cultured separately in the normal and hypoxic environment. The adhesion ability of Cell PG were detected by MTT. The invasion ability of Cell PG were detected by Transwell chamber. Results Observed on the reconstruction of the basement membrane adhesion in PG cells by MTT, in normoxic environment the mean absorbance of the TMP high, medium and low concentrations, cisplatin group, control group were (0.556±0.040),(0.519±0.056), (0.517±0.061),(0.333±0.057),(0.300±0.066), P<0.01. In hypoxic environment the mean absorbance of the TMP high , medium and low concentrations, cisplatin group, control group were (0.48±0.032),(0.452±0.059),(0.274±0.093),(0.464±0.099),(0.155±0.042), P<0.01. Promotion of cell adhesion with Tetramethylpyrazine of high, medium and low concentration group and DDP group in hypoxic environment is greater than in normoxic environment(P<0.01). Observed the reconstruction of the invasion of basement membrane of PG cells by Transwell chamber, in normoxic environment the invasive cells of the TMP high, medium and low concentrations, cisplatin group, control group were :(28.00±17.00),(91.67±30.37),(105.67±39.58),(85.00±8.19),(152.33±24.71), P<0.05. In hypoxic environment the invasive cells of the TMP high , medium and low concentrations , cisplatin group, control group were (20.33±15.31), (22.33±17.62),(56.00±11.53),(57.67±35.95), (76.33±18.01), P<0.05. The invasive cells of normoxic control group was significantly more than hypoxic control group(P<0.05). Conclusion In hypoxic environment , adhesion and invasion ability of Cell PG significantly decreased compared with normoxic environment ; In normoxia and hypoxia environment, Tetramethylpyrazine and cisplatin could promote the adhesion ability and inhibit the invasion ability of PG cells. Role in promoting of Tetramethylpyrazine on cell adhesion of PG cells was stronger in hypoxic environment.

The invasion and metastasis of tumor as the primary cause of death in cancer increases not only the patient's suffering, but also the difficulty of clinical treatment. It is an important guiding to find the molecular biological tumor markers and gene targets of antirecurrence and antimetastasis for clinical treatment. The invasion and metastasis of tumor is a complex process including the biological characteristics of the cancer, interaction among host cells, extracellular matrix and cancer cells, etc. Recently, epithelial mesenchymal transition (EMT) is becoming a hot spot in oncology research by virtue of its effect on genesis and development of tumor. Currently, the known markers of EMT include epithelial marker proteins mainly containing E-cadherin and interstitial marker proteins mainly containing N-cadherin and vimentin. It is necessary to make a summary among the studies on three proteins.

Objective To analyze the contemporary TCM academic team of lung cancer, summarizes its main academic thoughts. Methods To represent characters, important documents, and the main academic ideas as the basis of a team. From the perspective of the literature, literature of Chinese medicine against lung cancer, adopt the method of literature metrology, and social network analysis the candidate teams were found;the method of literature investigation evaluation candidate team heritage was adopted, the main academic thoughts from each team representative was summarized. Results A total of 8 441 researchers published 5 030 papers about treatment of lung cancer with TCM. 91 researchers with large amount papers and seven academic team were found. Conclusion Chinese medicine treatment of lung cancer made a great progress in the contemporary, team enriched the TCM system of principle, method, square, medicine.

0.05). Conclusion Deficiency of Qi, Blood stasis, Damp-phlegm and Deficiency of Yin are main syndromes of TCM in adenocarcinoma and squamous carcinoma. In Deficiency of Qi, lung-deficiency of Qi and spleen-deficiency of Qi are common syndromes of TCM. Appearing frequency of syndromes of TCM between adenocarcinoma and squamous carcinoma is similar.

Objective To investigate the distributing characteristics of blood stasis syndrome on 300 cases of advanced stage lung cancer patiens. Method Through filling in the table-syndrome of distributing characteristics of primary pulmonary carcinoma for stage Ⅲ and Ⅳ patients,the distributing characteristics of blood stasis syndrome in advanced lung cancer patiens were observed. Result The constituent ratio of blood stasis syndrome increased significantly following the worse patho-typing degree,the cell differentiation degree,the occurrence of hepatic metastasis,opposite pulmonary metastasis,osseous metastasis and troisier sign. Conclusion The syndrome of blood stasis of lung cancer has the distinct feature. Accordingly,the diagnostic standard which can be qualified of syndrome of blood stasis should be established. Only in that way can we guide the clinical diagnosis,the evaluation of therapeutic effect and the clinical trial of new drug of TCM preferably.

Objective To investigate the effect of edaravone on the JAK2/STAT3 signaling pathway after ischemia-reperfusion injury in donor rat liver under different cold ischemia times. Methods A total of 102 SD rats were randomly divided into sham operation group,control group and experimental group. Six rats were in sham operation group with free liver operation and no transplantation. Forty-eight rats were in control group and experimental group respectively, and divided into subgroups according to the different cold ischemia times (30 min, 6 h, 12 h and 18 h). There were 6 donors and 6 recipients in each group. The rat model of orthotopic liver transplantation was established by modifiedtwo cuff method. All the donors were perfused by abdominal aorta and the warm ischemia time was 3-5 min. After different cold ischemia times, the experimental group was treated with edaravone (3 mg/kg) at 5 min before the opening of the new hepatic artery, and control group was injected with 3 mg/kg saline. Recipients of each group were sacrificed after 6 h. Finally, real-time fluorescence quantitative PCR was used to analyze the relative expression of JAK2/STAT3 mRNA of donor liver. Results The GAPDH gene and JAK2/STAT3 were well amplified. Under the same cold ischemia time, compared with the control group, the relative expression of JAK2/STAT3 was significantly decreased in the experimental group (P<0.05). With the prolongation of cold ischemia time, the relative expressions of JAK2 and STAT3 mRNA showed a decreasing trend in control group and experimental group, while the relative expression of JAK2 mRNA increased first and then decreased in the experimental group (P<0.05). Conclusion Edaravone has a protective effect on transplanted donor liver during different cold ischemia times, and extends the cold ischemia time for 18 h, which may be related to the inhibition of JAK2/STAT3 signal transduction pathway.

Objective To observe the effects of different doses of Sappan Lignum and Chuanxiong Rhizoma on tumor stem cells marker ABCG2 in vivo. Methods Sphere cells obtained from serum culture were inoculated in nude mouse armpit, which were randomly divided into 5 groups: control group, Sappan Lignum high- and low-dose groups, and Chuanxiong Rhizoma high- and low-dose groups. Each medication group was given relevant medicine for gavage. 21 days later, inhibition tumor rate and ABCG2 protein and mRNA expression were detected with confocal microscope, Western blot, and RT-PCR. Results The sphere cells obtained from serum free culture had the abilities of cancer stem cells, such as proliferation, anti-aptosis and high expression of cancer stem cells markers. Chuanxiong Rhizoma high- and low-dose groups could inhibit tumor growth (P<0.05), and the inhibitory rate of Chuanxiong Rhizoma low-dose group was higher than the Chuanxiong Rhizoma high-dose group. Sappan Lignum high- and low-dose groups inhibited tumor growth without statistical significiance (P>0.05). Compared with the control group, Chuanxiong Rhizoma low-dose group could significantly inhibit the expression of resistant protein of ABCG2. Sappan Lignum high- and low-dose groups could not inhibit the protein expression of ABCG2. Each medication group up-regulated the mRNA expression of ABCG2 except for Chuanxiong Rhizoma low-dose group. Conclusion Low dose of Chuanxiong Rhizoma can inhibit the expression of ABCG2 protein levels, which can be the targeting killer for cancer stem cells.

Objective Observing the effect of Shuganyishen formula on hormone-dependant metastatic breast cancer and the mechanisms of anti-resistance and endocrine therapy. Methods 226 ITT cases were divided into two cohorts by treatment. 126 cases were treated with the combination of Shuganyishen formula and standard western medicine including endocrine therapy, and 100 cases were treated with western medicine alone. The end points were time to progress (TTP) and overall survival and quality of life (QOF). The effect of Shuganyishen formula on growth of TAM-resistance cell line in vitro was observed. Results There were statistical significances of median TTP between combination and western medicine in Luminal A (ER+ /PR+, HER2-) as well as Luminal B (ER+/PR+, HER2+), but no significances of OS; The extent and incidence of hot flush, sweat and syndromes related to bone were decreased significantly in combination treated group. Shuganyishen formula could reverse resistance to TAM. Conclusion Patients with LuminalA and LuminalB metastatic breast cancer benefit from Shuganyishen formula, which maybe related with its reverse resistance to endocrine therapy.

Objective To observe the effects of dredging collaterals and activating blood worm Chinese materia medica on angiogenesis related factors of lung cancer in hypoxic environment. Methods The lung cancer A549 cells were cultured in vitro to simulate tumor hypoxia microenvironment by the hypoxia workstation, and different concentrations of Scorpio, Scolopendra and Gecko medicated serum were added. MTT method was used to detect cell proliferation and screen the best medicine concentration and duration of action. Lung cancer A549 cells were administrated by the three kinds of medicated serum, and cells were collected and supernatant was cultured. Contents of VEGF, TGF-β1, and bFGF were detected by ELISA. Results Three kinds of medicated serum had the inhibitory effect on both added normoxia and hypoxia in cultured A549 lung cancer cells. 7.5% concentration of medicated serum was selected, and 24 h later were used in later experiments. Scorpio, Scolopendra and Gecko medicated serum can more reduce the contents of VEGF, TGF-β1 and bFGF in the supernatant of A549 cell compared with the control group (P<0.05, P<0.01). Conclusion Dredging collaterals and activating blood worm Chinese materia medica had inhibitory effect on cancer cells and the regulation of angiogenesis related cytokines in the condition of normoxia and hypoxia.