Objective To investigate the changes of serum high sensitivity C-reactive protein level (hs CRP)and albuminuria (ALB) level in patients with prehypertension and to investigate the relationship between hs CRP and ALB. Methods Total FBG,TC,TG,BMI and LDL were measured in 110 patients with prehypertension and 87 normal controls. Serum CRP and ALB concentration was measured by ELISA. Results Serum hs-CRP,urinary albumin concentration in patients with prehypertension was significantly high and had difference between male and female (P

Measles surveillance in Jiangsu Province started in 1999 by dividing the province into two sorts of areas. The first sort of area is economic well-developed area where EPI is carried out well and measles incidence is lower. The cities of this area investigated included Suzhou, Wuxi, Changzhou and Zhenjiang. The second sort of area included 9 cities (Nanjing, Xuzhou, Liangyungang, Nantong, Huaiyin, Suqian, Yangcheng, Yangzhou and Taizhou) where all the conditions are not as good as that mentioned in the first sort of area. The surveillance system of the province reported in 1999 a total of 938 suspected measles cases in 13 cities, of which 684 were confirmed as measles. The notifiable reporting system reported 846 suspected measles cases and 684 cases were confirmed. This indicated that the surveillance system operated well. The measles epidemiological features in two sorts of areas were compared. In first sort of area, including Nanjing, the measles cases of ≥15 years old accounted for 66.3% of the total cases. In second sort of area, not including Nanjing, the measles cases of <15 years old accounted for 90.8% of the total cases, and 73.2% of them were patients <10 years old. In 1999, there were 10 measles outbreaks occurred in the province and 218 measles cases were reported which accounted for 31.7% of the confirmed measles cases reported by the provincial measles surveillance system. 9% the 10 outbreaks occurred in the second sort of area, the cases were preschool and primary school children. The results showed that progression of EPI work was uneven in two sorts of areas of Jiangsu Province.

Objective To establish the HPLC fingerprint of Herba Selaginellae moellendorfii for identification and comparison with other species of the same genus and to determine the content of amentoflavone.Method Separation was performed on ZORBAX SB-C18 chromatographic column,acetonitrile(A)-0.5 %solution of acetic acid in water(B)as mobile phase with gradient elution and the flow rate was 1.0 mL?min-1.The detection wavelength was at 270 nm.The content determination of amentoflavone carried out synchronously.Results The characteristic eighteen peaks in the chromatogram consisted of the common pattern of Herba Selaginellae moellendorfii.The fingerprint coupling with similarity and Principal Component Analysis differentiated and classified the various species of selaginella.Nine species could be divided into 3 classes.The content of amentoflavone in Herba Selaginellae moellendorfii was 0.8～1.0 %.Conclusions The weighted similarity coefficient was calculated from 13 batches of Herba Selaginellae moellendorfii samples as high as more than 0.98.Among 9 species of Selaginella,5 species(S.biformis,S.involvens,S.doederileinii,S.trachyphylla,S.tamariscina)were sorted out by means of Principal Component Analysis in the same class with S.moellendorfii.Which indicated the possibility of bio-equivalence among the herbs of these six species,while S.picta,S.uncinata and S.delicatula are considered as adulterants of S.moellendorfii as their dissimilar fingerprints.

AIM: Terminalia chebula contained hydrolysable tannins up to about 35%. It was necessary to establish a chromatographic fingerprint to meet the quality control need effectively. METHODS: HPLC method was carried out with 3 kinds of the mobile phase , namely, A∶ 0.05mol?L -1 Phosphoric acid/ 0.05mol?L -1 Potassium dihydrogen phosphorate aqueous solution, B: methanol and C: Ethyl acetate, running in gradient mode based on the previous experiment. RESULTS: A marked peaks of HPLC fingerprint of the raw material, the extracts and its final product consisted of gallic acid, terchebulin, chebulamin, chebulagic acid and chebulinic acid. CONCLUSION: The fact has depicted that chromatographic fingerprint is a powerful tool for in-process-quality supervisory control and dynamic analysis of the active constituents during manufacture procedure of immature fruit products of Terminalia chebula.

Objective: To investigate the immunity to mumps after administrating measles-mumps-rubella vaccine (MMR) among children aged 2-7 years old in Jiangsu province in 2015. Methods: A total of 4 190 healthy children aged 2-7 years old, living in local places for at least 3 months, and having been vaccinated at least 1 dose MMR were recruited to the study from Wujin district of Changzhou city, Gaogang district of Taizhou city and Ganyu district of Lianyungang city by using stratified cluster random sampling method between September and November, 2015. Those who did not accept MMR vaccination, who refused venous blood collection, who had affected mumps according to the memory of parents or teachers and who were diagnosed serious disease by clinical doctors were excluded from study. The self-designed questionnaire was used to collect the general information of the subjects and their MMR immunization history; and 0.5-2.0 ml of venous blood was collected from each subject. ELISA was used to detect the mumps antibody level in the serum of patients. Positive was defined as the antibody level ≥108 mU/ml, and negative as <108 mU/ml. χ² test was used to compare the difference in positive rates among subjects; and analysis of variance was used to compare the GMC changes in different time points after MMR vaccination. Results: Among 4 190 children, 2 280 were males (54.42%) and 1 910 were females(45.58%), and the positive rate of IgG antibody was 81.38% (3 344). There were 3 156 (95.18%) children vaccinated with one dose MMR, 187 (4.80%) children with two dose MMR, and 1 (0.02%) child with three dose MMR. The difference in positive rate of IgG antibody among different aged subjects showed statistical significance (χ²=58.61, P<0.001), the highest positive rate was in group of subjects aged 4-5 years old, at 89.43% (406/454), while the lowest positive rate was found among subjects aged 6-7 years old, at 75.63% (1 648/2 179). The positive rate after one dose of MMR vaccination was 79.14% (3 156/3 988), significantly less than it after two doses (93.03%, 187/201) (χ²=22.93, P<0.001). The GMC level at years<1, 1-<2, 2-<3, 3-<4, ≥4 following one dose MMR in the 3 988 children was 152.47, 227.78, 167.08, 126.91, 79.43 mU/ml, whose difference was statistically significant (F=51.29, P<0.001). Conclusion The sero-prevalence of IgG antibody in the children aged 2-7 years old in Jiangsu province was high. The positive rate among who received two doses MMR was significantly higher than it among who received just one dose, and the GMC level waned with times.

Objective To study the effects of different pellet feed hardness on the growth and reproduction, feed utilization rate, and environmental dust in laboratory mice.Methods One hundred of fifty 50 3-week-old SPF-grade C57BL/6JGpt and 150 ICR laboratory mice were randomly divided into three groups, with an equal number of males and females. They were fed diets with different hardness of 18.62 kg, 23.15 kg, and 27.89 kg. Body weight, feed utilization rate, and dust levels in cages were recorded and calculated for mice aged 3-10 weeks. Forty-five 6-week-old male mice and ninety 4-week-old female mice from each strain were randomly divided into three groups and fed pellet feeds with three different hardness levels. After 2 weeks of adaptation to the same hardness feed, the mice were paired at a 1∶2 male-to-female ratio and monitored for reproductive data for 3 months.Results At the age of 4 weeks, the body weight of male C57BL/6JGpt mice in 23.15 kg group was significantly higher than that in the 18.62 kg and 27.89 kg groups (P<0.01), and the body weight of females in the 18.62 kg group was significantly higher than that in the 27.89 kg group (P<0.05). There was no significant difference in body weight among ICR mice aged 3-10 weeks across different feed hardness groups (P>0.05). For both strains, feed utilization rate for males was higher than that for females across different feed hardness groups at all weeks of age (P<0.01). Compared to the 27.89 kg group, both the 18.62 kg and 23.15 kg groups showed a significant increase in the 50-mesh dust levels in cages for both strains aged 4-8 weeks (except for 7-week-old C57BL/6JGpt mice) (P<0.05). For both C57BL/6JGpt and ICR mice, there was no significant difference in basic reproductive performance such as interval between the first litter and the monthly production index among the three feed hardness groups during the experimental period (P>0.05). However, the monthly production index of C57BL/6JGpt mice first increased and then decreased with the increase of feed hardness, while that of ICR mice increased with increasing feed hardness, though these differences were not statistically significant (P>0.05).Conclusion Different strains and genders had different tolerance to feed hardness. C57BL/6JGpt mice are more adapted to lower hardness feeds, while ICR mice are better suited to slightly higher hardness feeds.