Objective To establish the standard of quality control for Pericarpium Citri Reticulatae Viride (PCRV) and to identify two kinds of PCRV which are collected in different times. Methods In this article,volatile components and flavonoid in 10 batches of samples which were collected from eight provinces were determined by GC and HPLC-DAD,respectively. Their fingerprints were handled by weighted similarity of range and by principal component analysis (PCA). Results According to the result of similarity and classification,there were obvious differences between the two kinds of PCRV.To know whether the differences have great influences on the pharmacological actions of PCRV, pharmacological studies will be needed. Weighted similarity is suitable for this system which has a high content of similar components. Conclusion These methods are effective and will help us to establish the criterion of quality control for PCRV.

Objective:To explore the effects of Zusanli acupoint massage combined with abdominal touching on feeding intolerance (FI) of premature infants. Methods:A total of 100 premature infants meeting the inclusion criteria in neonatal ward of the hospital from January 2018 to June 2020 were divided into control group (50 cases) and observation group (50 cases) by random number table method. The control group was given routine treatment and feeding, while observation group was given Zusanli acupoint massage and abdominal touching on the basis of control group. The feeding observation indexes, three immunoglobulin indexes, FI and complications were compared between the two groups. Results:The feeding transition time (8.57 ± 2.35 d vs. 11.60 ± 4.38 d, t=4.310) and the time of returning to birth weight (9.11 ± 1.79 d vs. 10.48 ± 2.34 d, t=3.289) in observation group were significantly shorter than those in control group ( P<0.05), and feeding efficiency (9.48 ± 2.07 ml/min vs. 7.16 ± 2.30 ml/min, t=5.302) was significantly higher than that of control group ( P<0.05). The incidence rates of FI [14.0% (7/50) vs. 42.0% (21/50); χ2=9.722, P<0.01] and apnea [12.0% (6/50) vs. 32.0% (16/51); χ2=5.828, P<0.01]. Conclusion:Zusanli acupoint massage combined with abdominal touching is beneficial to improve feeding performance of premature infants, recover birth weight as soon as possible, achieve total intestinal feeding and improve early feeding tolerance.

The oral cavity of each person is home to hundreds of bacterial species.While taxa for oral diseases have been studied using culture-based characterization as well as amplicon sequencing,metagenomic and genomic information remains scarce compared to the fecal microbiome.Here,using metagenomic shotgun data for 3346 oral metagenomic samples together with 808 published samples,we obtain 56,213 metagenome-assembled genomes(MAGs),and more than 64％of the 3589 species-level genome bins(SGBs)contain no publicly available genomes.The resulting genome collection is representative of samples around the world and contains many genomes from candi-date phyla radiation(CPR)that lack monoculture.Also,it enables the discovery of new taxa such as a genus Candidatus Bgiplasma within the family Acholeplasmataceae.Large-scale metagenomic data from massive samples also allow the assembly of strains from important oral taxa such as Por-phyromonas and Neisseria.The oral microbes encode genes that could potentially metabolize drugs.Apart from these findings,a strongly male-enriched Campylobacter species was identified.Oral sam-ples would be more user-friendly collected than fecal samples and have the potential for disease diagnosis.Thus,these data lay down a genomic framework for future inquiries of the human oral microbiome.