OBJECTIVE To investigate the resistance gene spectrum and their relatedness in Enterobacter cloacae isolated from sputum.METHODS The 15 kinds of resistant genes were detected by polymerase chain reaction and cluster analysis was done.RESULTS The positive rates of TEM,OXA-1 group,DHA,ACT-1,aac(6′)-Ⅰ,ant(3″)-Ⅰ,qnr,dfrA17,sul1 and intⅠ1 were 78.6%,6.3%,18.8%,75.0%,78.6%,56.3%,6.3%,37.5%,87.5% and 87.5%,respectively.Clone transmission was occurred.CONCLUSIONS There are very high positive rates of TEM,DHA,ACT-1,aac(6′)-Ⅰ,ant(3″)-Ⅰ,dfrA17,sul1 and intⅠ1 genes of E.cloacae isolated from sputum.E.cloacae can be clone transmitted in the hospital.

OBJECTIVE To investigate the resistance of Enterobacter cloacae and its gene of chlorhexidine-sulfadiazine-resistance(qacE△1-sul1) isolated from two hospitals. METHODS Microdilute tests were performed to detect the susceptibility of 20 kinds of antimicrobial agents in 74 strains of E.cloacae.The qacE△1-sul1gene was detected by PCR. RESULTS There was no strain resistant to imipenem and meropenem.The resistant rates to other antimicrobial agents were between 44.6% and 94.6%.The positive rate of qacE△1-sul1gene was 74.3%. CONCLUSIONS The 74 strains were multiple-drug-resistant.There was a high positive rate of qacE△1-sul1gene in E.cloacae isolated from two hospitals.

OBJECTIVE To investigate the prevalence of three kinds of antimicrobial-resistant genes in Enterobacter cloacae. METHODS Antimicrobial susceptibility test was performed by microdiluted method and antimicrobial - resistant genes were detected by PCR methods in 20 clinical strains of E. cloacae. RESULTS Twenty strains of E. cloacae showed multiple-drug resistance.Among 20 strains, the positive rates of ACT-1, DHA and TEM genes were 70.0%, 45.0% and 55.0%, respectively. The positive rates of aac(6′) -Ⅰ , ant(3″)-Ⅰ, ant(2″)-Ⅰ, aac(6′)-Ⅱ, and aac(3)-Ⅱgenes were 50.0%, 15.0%, 10.0%, 5.0% and 5.0%, respectively . The positive rates of sul Ⅰand dfrA1 genes were 50.0%, and 5.0%, respectively. But other genes were not found. CONCLUSIONS This study shows that there are multiple-drug resistance and high positive rate of three kinds of antimicrobial- resistant genes in E. cloacae isolated from our hospital.